Abstract：
:In mammals, inactivation of one X chromosome in the female equalizes gene dosages between XX females and XY males. Two noncoding loci, Tsix and Xite, together regulate X chromosome fate by controlling homologous chromosome pairing, counting, and mutually exclusive choice. Following choice, the asymmetry of Xite and Tsix expression drives divergent chromosome fates, but how this pattern becomes established is currently unknown. Although no proven trans-acting factors have been identified, a likely candidate is Ctcf, a chromatin insulator with essential function in autosomal imprinting. Here, we search for trans-factors and identify Yy1 as a required cofactor for Ctcf. Paired Ctcf-Yy1 elements are highly clustered within the counting/choice and imprinting domain of Tsix. A deficiency of Yy1 leads to aberrant Tsix and Xist expression, resulting in a deficit of male and female embryos. Yy1 and Ctcf associate through specific protein-protein interactions and together transactivate Tsix. We propose that the Ctcf-Yy1-Tsix complex functions as a key component of the X chromosome binary switch.
journal_name
Mol Celljournal_title
Molecular cellauthors
Donohoe ME,Zhang LF,Xu N,Shi Y,Lee JTdoi
10.1016/j.molcel.2006.11.017subject
Has Abstractpub_date
2007-01-12 00:00:00pages
43-56issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(06)00788-Xjournal_volume
25pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract：:One of the earliest steps in metazoan pre-mRNA splicing involves binding of U2 snRNP auxiliary factor (U2AF) 65 KDa subunit to the polypyrimidine (Py) tract and of the 35 KDa subunit to the invariant AG dinucleotide at the intron 3' end. Here we use in vitro and in vivo depletion, as well as reconstitution assays usin...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.11.033
更新日期：2012-02-10 00:00:00
abstract：:Faithful transmission of genomic information requires tight spatiotemporal regulation of DNA replication factors. In the licensing step of DNA replication, CDT-1 is loaded onto chromatin to subsequently promote the recruitment of additional replication factors, including CDC-45 and GINS. During the elongation step, th...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.08.028
更新日期：2011-10-07 00:00:00
abstract：:EGFR activates phosphatidylinositide 3-kinase (PI3K), but the mechanism underlying this activation is not completely understood. We demonstrated here that EGFR activation resulted in lysine acetyltransferase 5 (KAT5)-mediated K395 acetylation of the platelet isoform of phosphofructokinase 1 (PFKP) and subsequent trans...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.03.018
更新日期：2018-04-19 00:00:00
abstract：:The eukaryotic RNA polymerases Pol I, II, and III use different promoters to transcribe different classes of genes. Promoter usage relies on initiation factors, including TFIIF and TFIIE, in the case of Pol II. Here, we show that the Pol I-specific subunits A49 and A34.5 form a subcomplex that binds DNA and is related...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.07.028
更新日期：2010-08-27 00:00:00
abstract：:Integration of the mycobacteriophage Bxb1 genome into its host chromosome is catalyzed by a serine-integrase, a member of the transposon-resolvase family of site-specific recombinases. These enzymes use a concerted mechanism of strand exchange involving double-stranded cleavages with two-base extensions, and covalent ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00444-1
更新日期：2003-11-01 00:00:00
abstract：:tRNAs reading four-codon families often have a modified uridine, cmo(5)U(34), at the wobble position of the anticodon. Here, we examine the effects on the decoding mechanism of a cmo(5)U modification in tRNA(1B)(Ala), anticodon C(36)G(35)cmo(5)U(34). tRNA(1B)(Ala) reads its cognate codons in a manner that is very simi...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.11.014
更新日期：2007-01-12 00:00:00
abstract：:Autophagy constitutes a prominent mechanism through which eukaryotic cells preserve homeostasis in baseline conditions and in response to perturbations of the intracellular or extracellular microenvironment. Autophagic responses can be relatively non-selective or target a specific subcellular compartment. At least in ...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2015.07.021
更新日期：2015-08-20 00:00:00
abstract：:Histone H2B monoubiquitination (H2Bub1) is centrally involved in gene regulation. The deubiquitination module (DUBm) of the SAGA complex is a major regulator of global H2Bub1 levels, and components of this DUBm are linked to both neurodegenerative diseases and cancer. Unexpectedly, we find that ablation of USP22, the ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.03.030
更新日期：2016-05-19 00:00:00
abstract：:A kinase-anchoring proteins (AKAPs) target PKA to specific microdomains by using an amphipathic helix that docks to N-terminal dimerization and docking (D/D) domains of PKA regulatory (R) subunits. To understand specificity, we solved the crystal structure of the helical motif from D-AKAP2, a dual-specific AKAP, bound...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.09.015
更新日期：2006-11-03 00:00:00
abstract：:Activating mutations in NOTCH1, an essential regulator of T cell development, are frequently found in human T cell acute lymphoblastic leukemia (T-ALL). Despite important advances in our understanding of Notch signal transduction, the regulation of Notch functions in the nucleus remains unclear. Using immunoaffinity p...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.08.022
更新日期：2012-11-09 00:00:00
abstract：:Concomitant with DNA replication, the chromosomal cohesin complex establishes cohesion between newly replicated sister chromatids. Several replication-fork-associated "cohesion establishment factors," including the multifunctional Ctf18-RFC complex, aid this process in as yet unknown ways. Here, we show that Ctf18-RFC...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.03.017
更新日期：2020-05-21 00:00:00
abstract：:In the Neurospora circadian system, the transcription factors White Collar-1 (WC-1) and White Collar-2 (WC-2) activate expression of frq, whose gene product inhibits its own expression. The WC proteins are thought to form an obligate complex; however, chromatin immunoprecipitation (ChIP) indicates that WC-2 binds to t...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.01.010
更新日期：2007-02-23 00:00:00
abstract：:Ubiquitin modification of proteins plays pivotal roles in the cellular response to DNA damage. Given the complexity of ubiquitin conjugation due to the formation of poly-conjugates of different linkages, functional roles of linkage-specific ubiquitin modification at DNA damage sites are largely unclear. We identify th...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2017.04.013
更新日期：2017-05-18 00:00:00
abstract：:In this issue of Molecular Cell, Miotto and Struhl (2010) suggest that replication licensing, the loading of Mcm2-7 onto DNA, is promoted by HBO1 acetylating histone H4 at replication origins, providing a molecular view of how chromatin status influences origin usage. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2009.12.028
更新日期：2010-01-15 00:00:00
abstract：:The proteolytic activation of the membrane-associated transcription factor pro-sigma(K) is controlled by a signal transduction pathway during sporulation in the bacterium Bacillus subtilis. The pro-sigma(K) processing enzyme SpoIVFB, a membrane-embedded metalloprotease, is held inactive by two other integral-membrane ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.05.019
更新日期：2006-07-07 00:00:00
abstract：:Interference with nucleocytoplasmic transport is a strategy employed by certain viruses to compromise host cellular function. While it has been shown that the matrix (M) protein of the vesicular stomatitis virus (VSV) inhibits nuclear export of host cell mRNAs, the underlying mechanism has not been fully established. ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2004.11.023
更新日期：2005-01-07 00:00:00
abstract：:The diversity of mRNA lifetimes in bacterial cells is difficult to reconcile with the relaxed cleavage site specificity of RNase E, the endonuclease most important for governing mRNA degradation. This enzyme has generally been thought to locate cleavage sites by searching freely in three dimensions. However, our resul...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.01.044
更新日期：2019-04-18 00:00:00
abstract：:Budding yeast Cdc13, Stn1, and Ten1 form the CST complex to protect telomeres from lethal DNA degradation. It remains unknown whether similar complexes are conserved in higher eukaryotes or not. Here we isolated mammalian STN1 and TEN1 homologs and CTC1 (conserved telomere maintenance component 1). The three proteins ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.08.009
更新日期：2009-10-23 00:00:00
abstract：:In a recent issue of Molecular Cell, Kretov et al. (2020) demonstrate that microRNA-144 targets Dicer in a negative feedback loop, affecting global canonical microRNA expression in erythrocytes. MicroRNA-451 is refractory to the loss of Dicer because of its Ago2-dependent processing. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2020.04.028
更新日期：2020-06-04 00:00:00
abstract：:The structure of an RNA polymerase II/general transcription factor TFIIF complex was determined by cryo-electron microscopy and single particle analysis. Density due to TFIIF was not concentrated in one area but rather was widely distributed across the surface of the polymerase. The largest subunit of TFIIF interacted...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00387-3
更新日期：2003-10-01 00:00:00
abstract：:The positive transcription elongation factor b (P-TEFb) is a cyclin-dependent kinase that controls the elongation phase of transcription by RNA polymerase II (RNAPII). This process is made possible by the reversal of effects of negative elongation factors that include NELF and DSIF. In complex organisms, elongation co...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2006.06.014
更新日期：2006-08-04 00:00:00
abstract：:To unravel the roles of LXRs in inflammation and immunity, we examined the function of LXRs in development of IFN-gamma-mediated inflammation using cultured rat brain astrocytes. LXR ligands inhibit neither STAT1 phosphorylation nor STAT1 translocation to the nucleus but, rather, inhibit STAT1 binding to promoters and...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.07.021
更新日期：2009-09-24 00:00:00
abstract：:Recent work from Kuehner and Brow (2008) and Thiebaut et al. (2008) in Molecular Cell and Jenks et al. (2008) in Molecular and Cellular Biology reveals that regulated expression of central nucleotide synthesis pathway components directs start site-dependent RNA polymerase II termination. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2008.08.014
更新日期：2008-09-05 00:00:00
abstract：:We have identified ARAP1 and ARAP2 and examined ARAP1 as a possible link between phosphoinositide-, Arf-, and Rho-mediated cell signaling. ARAP1 contains Arf GAP, Rho GAP, Ankyrin repeat, Ras-associating, and five PH domains. In vitro, ARAP1 had Rho GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent A...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00428-8
更新日期：2002-01-01 00:00:00
abstract：:Microtubule plus end binding proteins (+TIPs) localize to the dynamic plus ends of microtubules, where they stimulate microtubule growth and recruit signaling molecules. Three main +TIP classes have been identified (XMAP215, EB1, and CLIP-170), but whether they act upon microtubule plus ends through a similar mechanis...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.07.023
更新日期：2007-09-21 00:00:00
abstract：:In a recent issue of Molecular Cell, Büttner et al. (2007) described dual roles for the conserved mitochondrial protein endonuclease G in budding yeast apoptosis and proliferation that illuminate some of the contradictory and confusing findings regarding this protein in mammalian cells. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.molcel.2007.01.030
更新日期：2007-02-09 00:00:00
abstract：:SOCS3 is essential for regulating the extent, duration, and specificity of cellular responses to cytokines such as G-CSF and IL-6. Here we describe the solution structure of SOCS3, the first structure determined for any SOCS protein, in complex with a phosphotyrosine-containing peptide from the IL-6 receptor signaling...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.03.024
更新日期：2006-04-21 00:00:00
abstract：:The RNA-binding protein Sam68 is implicated in various cellular processes including RNA metabolism, apoptosis, and signal transduction. Here we identify a role of Sam68 in TNF-induced NF-κB activation and apoptosis. We found that Sam68 is recruited to the TNF receptor, and its deficiency dramatically reduces RIP recru...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.05.007
更新日期：2011-07-22 00:00:00
abstract：:Homologous recombination is essential for preserving genome integrity. Joining of homologous DNA molecules through strand exchange, a pivotal step in recombination, is mediated by RAD51. Here, we identify RAD51AP1 as a RAD51 accessory protein that specifically stimulates joint molecule formation through the combinatio...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.08.025
更新日期：2007-11-09 00:00:00
abstract：:Calreticulin and calnexin are homologous lectins that serve as molecular chaperones for glycoproteins in the endoplasmic reticulum of eukaryotic cells. Here we show that calreticulin depletion specifically accelerates the maturation of cellular and viral glycoproteins with a modest decrease in folding efficiency. Caln...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00494-5
更新日期：2004-01-16 00:00:00
