Division of Labor between PCNA Loaders in DNA Replication and Sister Chromatid Cohesion Establishment.

Abstract:

:Concomitant with DNA replication, the chromosomal cohesin complex establishes cohesion between newly replicated sister chromatids. Several replication-fork-associated "cohesion establishment factors," including the multifunctional Ctf18-RFC complex, aid this process in as yet unknown ways. Here, we show that Ctf18-RFC's role in sister chromatid cohesion correlates with PCNA loading but is separable from its role in the replication checkpoint. Ctf18-RFC loads PCNA with a slight preference for the leading strand, which is dispensable for DNA replication. Conversely, the canonical Rfc1-RFC complex preferentially loads PCNA onto the lagging strand, which is crucial for DNA replication but dispensable for sister chromatid cohesion. The downstream effector of Ctf18-RFC is cohesin acetylation, which we place toward a late step during replication maturation. Our results suggest that Ctf18-RFC enriches and balances PCNA levels at the replication fork, beyond the needs of DNA replication, to promote establishment of sister chromatid cohesion and possibly other post-replicative processes.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Liu HW,Bouchoux C,Panarotto M,Kakui Y,Patel H,Uhlmann F

doi

10.1016/j.molcel.2020.03.017

subject

Has Abstract

pub_date

2020-05-21 00:00:00

pages

725-738.e4

issue

4

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(20)30165-9

journal_volume

78

pub_type

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