Abstract:
:Concomitant with DNA replication, the chromosomal cohesin complex establishes cohesion between newly replicated sister chromatids. Several replication-fork-associated "cohesion establishment factors," including the multifunctional Ctf18-RFC complex, aid this process in as yet unknown ways. Here, we show that Ctf18-RFC's role in sister chromatid cohesion correlates with PCNA loading but is separable from its role in the replication checkpoint. Ctf18-RFC loads PCNA with a slight preference for the leading strand, which is dispensable for DNA replication. Conversely, the canonical Rfc1-RFC complex preferentially loads PCNA onto the lagging strand, which is crucial for DNA replication but dispensable for sister chromatid cohesion. The downstream effector of Ctf18-RFC is cohesin acetylation, which we place toward a late step during replication maturation. Our results suggest that Ctf18-RFC enriches and balances PCNA levels at the replication fork, beyond the needs of DNA replication, to promote establishment of sister chromatid cohesion and possibly other post-replicative processes.
journal_name
Mol Celljournal_title
Molecular cellauthors
Liu HW,Bouchoux C,Panarotto M,Kakui Y,Patel H,Uhlmann Fdoi
10.1016/j.molcel.2020.03.017subject
Has Abstractpub_date
2020-05-21 00:00:00pages
725-738.e4issue
4eissn
1097-2765issn
1097-4164pii
S1097-2765(20)30165-9journal_volume
78pub_type
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