ARAP1: a point of convergence for Arf and Rho signaling.

Abstract:

:We have identified ARAP1 and ARAP2 and examined ARAP1 as a possible link between phosphoinositide-, Arf-, and Rho-mediated cell signaling. ARAP1 contains Arf GAP, Rho GAP, Ankyrin repeat, Ras-associating, and five PH domains. In vitro, ARAP1 had Rho GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent Arf GAP activity. ARAP1 associated with the Golgi. The Rho GAP activity mediated cell rounding and loss of stress fibers when ARAP1 was overexpressed. The Arf GAP activity mediated changes in the Golgi apparatus and the formation of filopodia, the latter a consequence of increased cellular activity of Cdc42. The Arf GAP and Rho GAP activities both contributed to inhibiting cell spreading. Thus, ARAP1 is a PIP3-dependent Arf GAP that regulates Arf-, Rho-, and Cdc42-dependent cell activities.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Miura K,Jacques KM,Stauffer S,Kubosaki A,Zhu K,Hirsch DS,Resau J,Zheng Y,Randazzo PA

doi

10.1016/s1097-2765(02)00428-8

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

109-19

issue

1

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(02)00428-8

journal_volume

9

pub_type

杂志文章
  • Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function.

    abstract::Apoptosis is initiated when Bcl-2 and its prosurvival relatives are engaged by proapoptotic BH3-only proteins via interaction of its BH3 domain with a groove on the Bcl-2-like proteins. These interactions have been considered promiscuous, but our analysis of the affinity of eight BH3 peptides for five Bcl-2-like prote...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2004.12.030

    authors: Chen L,Willis SN,Wei A,Smith BJ,Fletcher JI,Hinds MG,Colman PM,Day CL,Adams JM,Huang DC

    更新日期:2005-02-04 00:00:00

  • miR-200c regulates induction of apoptosis through CD95 by targeting FAP-1.

    abstract::Tumor progression shares many characteristics with the process of epithelial-to-mesenchymal transition (EMT). Cells that have undergone an EMT are known to have an increased resistance to apoptosis. CD95/Fas is an apoptosis-inducing receptor expressed on many tissues and tumor cells. During tumor progression CD95 is f...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2010.05.018

    authors: Schickel R,Park SM,Murmann AE,Peter ME

    更新日期:2010-06-25 00:00:00

  • Smad about E2F. TGFbeta repressionof c-Myc via a Smad3/E2F/p107 complex.

    abstract::Signaling by TGFbeta regulates the expression of hundreds of genes. The rapid repression of c-Myc stands out because of its roles in growth control and cancer. Recent work shows that c-Myc repression by TGFbeta is mediated by the nuclear translocation of a novel, preformed complex composed of Smad3, E2F4 or E2F5, and ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(02)00584-1

    authors: Kowalik TF

    更新日期:2002-07-01 00:00:00

  • Different aa-tRNAs are selected uniformly on the ribosome.

    abstract::Ten E. coli aminoacyl-tRNAs (aa-tRNAs) were assessed for their ability to decode cognate codons on E. coli ribosomes by using three assays that evaluate the key steps in the decoding pathway. Despite a wide variety of structural features, each aa-tRNA exhibited similar kinetic and thermodynamic properties in each assa...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2008.04.026

    authors: Ledoux S,Uhlenbeck OC

    更新日期:2008-07-11 00:00:00

  • A bidirectional origin of replication maps to the major noncoding region of human mitochondrial DNA.

    abstract::In solid tissues of vertebrates, initiation of mitochondrial DNA replication encompasses a broad zone downstream of the major noncoding region (NCR). In contrast, analysis with two-dimensional agarose gel electrophoresis of mitochondrial DNA replication intermediates in cultured human cells revealed initiation concent...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2005.05.002

    authors: Yasukawa T,Yang MY,Jacobs HT,Holt IJ

    更新日期:2005-06-10 00:00:00

  • The stress-activated Hog1 kinase is a selective transcriptional elongation factor for genes responding to osmotic stress.

    abstract::Regulation of gene expression by stress-activated protein kinases (SAPKs) is essential for cell adaptation to extracellular stimuli. Exposure of yeast to high osmolarity results in activation of the SAPK Hog1, which associates with transcription factors bound at target promoters and stimulates transcriptional initiati...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2006.05.031

    authors: Proft M,Mas G,de Nadal E,Vendrell A,Noriega N,Struhl K,Posas F

    更新日期:2006-07-21 00:00:00

  • Yeast unravels epigenetic apoptosis control: deadly chat within a histone tail.

    abstract::An important paper by David Allis and colleagues (Ahn et al., 2006 [this issue of Molecular Cell]) describes a specific deacetylation/phosphorylation crosstalk at the histone H2B tail required for apoptosis induction in yeast, thus giving first insights into the poorly understood epigenetic regulation of cell death. ...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章,评审

    doi:10.1016/j.molcel.2006.10.004

    authors: Carmona-Gutierrez D,Madeo F

    更新日期:2006-10-20 00:00:00

  • MeCP2 Represses Enhancers through Chromosome Topology-Associated DNA Methylation.

    abstract::The genomes of mammalian neurons contain uniquely high levels of non-CG DNA methylation that can be bound by the Rett syndrome protein, MeCP2, to regulate gene expression. How patterns of non-CG methylation are established in neurons and the mechanism by which this methylation works with MeCP2 to control gene expressi...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2019.10.033

    authors: Clemens AW,Wu DY,Moore JR,Christian DL,Zhao G,Gabel HW

    更新日期:2020-01-16 00:00:00

  • A hedgehog-insensitive form of patched provides evidence for direct long-range morphogen activity of sonic hedgehog in the neural tube.

    abstract::Cell pattern in the ventral neural tube is organized by Sonic hedgehog (Shh) secreted by floor plate cells. To assay the range of direct Shh action, we developed a general method for blocking transduction of Hedgehog (Hh) signals through ectopic expression of a deleted form of the Hh receptor Patched (Ptc), termed Ptc...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(01)00271-4

    authors: Briscoe J,Chen Y,Jessell TM,Struhl G

    更新日期:2001-06-01 00:00:00

  • PKC regulates a farnesyl-electrostatic switch on K-Ras that promotes its association with Bcl-XL on mitochondria and induces apoptosis.

    abstract::K-Ras associates with the plasma membrane (PM) through farnesylation that functions in conjunction with an adjacent polybasic sequence. We show that phosphorylation by protein kinase C (PKC) of S181 within the polybasic region promotes rapid dissociation of K-Ras from the PM and association with intracellular membrane...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2006.01.012

    authors: Bivona TG,Quatela SE,Bodemann BO,Ahearn IM,Soskis MJ,Mor A,Miura J,Wiener HH,Wright L,Saba SG,Yim D,Fein A,Pérez de Castro I,Li C,Thompson CB,Cox AD,Philips MR

    更新日期:2006-02-17 00:00:00

  • ChIC and ChEC; genomic mapping of chromatin proteins.

    abstract::To map the genomic interaction sites of chromatin proteins, two related methods were developed and experimentally explored in Saccharomyces cerevisiae. The ChIC method (chromatin immunocleavage) consists of tethering a fusion protein (pA-MN) consisting of micrococcal nuclease (MN) and staphylococcal protein A to speci...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2004.09.007

    authors: Schmid M,Durussel T,Laemmli UK

    更新日期:2004-10-08 00:00:00

  • Identification of a major determinant for serine-threonine kinase phosphoacceptor specificity.

    abstract::Eukaryotic protein kinases are generally classified as being either tyrosine or serine-threonine specific. Though not evident from inspection of their primary sequences, many serine-threonine kinases display a significant preference for serine or threonine as the phosphoacceptor residue. Here we show that a residue lo...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2013.11.013

    authors: Chen C,Ha BH,Thévenin AF,Lou HJ,Zhang R,Yip KY,Peterson JR,Gerstein M,Kim PM,Filippakopoulos P,Knapp S,Boggon TJ,Turk BE

    更新日期:2014-01-09 00:00:00

  • BRCA1 recruitment to transcriptional pause sites is required for R-loop-driven DNA damage repair.

    abstract::The mechanisms contributing to transcription-associated genomic instability are both complex and incompletely understood. Although R-loops are normal transcriptional intermediates, they are also associated with genomic instability. Here, we show that BRCA1 is recruited to R-loops that form normally over a subset of tr...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2015.01.011

    authors: Hatchi E,Skourti-Stathaki K,Ventz S,Pinello L,Yen A,Kamieniarz-Gdula K,Dimitrov S,Pathania S,McKinney KM,Eaton ML,Kellis M,Hill SJ,Parmigiani G,Proudfoot NJ,Livingston DM

    更新日期:2015-02-19 00:00:00

  • Linking differential chromatin loops to transcriptional decisions.

    abstract::GATA-1 and GATA-2 control proliferation and differentiation of hematopoietic progenitor cells via transcriptional regulation. In this issue of Molecular Cell, Jing et al. (2008) demonstrate that GATA factor exchange on the Kit locus directs a transcriptional switch by reconfiguring chromatin loops. ...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2008.01.008

    authors: Apostolou E,Thanos D

    更新日期:2008-02-01 00:00:00

  • RPA Mediates Recruitment of MRX to Forks and Double-Strand Breaks to Hold Sister Chromatids Together.

    abstract::The Mre11-Rad50-Xrs2 (MRX) complex is related to SMC complexes that form rings capable of holding two distinct DNA strands together. MRX functions at stalled replication forks and double-strand breaks (DSBs). A mutation in the N-terminal OB fold of the 70 kDa subunit of yeast replication protein A, rfa1-t11, abrogates...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2016.10.032

    authors: Seeber A,Hegnauer AM,Hustedt N,Deshpande I,Poli J,Eglinger J,Pasero P,Gut H,Shinohara M,Hopfner KP,Shimada K,Gasser SM

    更新日期:2016-12-01 00:00:00

  • RNA polymerase I contains a TFIIF-related DNA-binding subcomplex.

    abstract::The eukaryotic RNA polymerases Pol I, II, and III use different promoters to transcribe different classes of genes. Promoter usage relies on initiation factors, including TFIIF and TFIIE, in the case of Pol II. Here, we show that the Pol I-specific subunits A49 and A34.5 form a subcomplex that binds DNA and is related...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2010.07.028

    authors: Geiger SR,Lorenzen K,Schreieck A,Hanecker P,Kostrewa D,Heck AJ,Cramer P

    更新日期:2010-08-27 00:00:00

  • Mitochondrial disulfide bond formation is driven by intersubunit electron transfer in Erv1 and proofread by glutathione.

    abstract::The disulfide relay system in the intermembrane space of mitochondria is of crucial importance for mitochondrial biogenesis. Major players in this pathway are the oxidoreductase Mia40 that oxidizes substrates and the sulfhydryl oxidase Erv1 that reoxidizes Mia40. To analyze in detail the mechanism of this oxidative pa...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2010.01.017

    authors: Bien M,Longen S,Wagener N,Chwalla I,Herrmann JM,Riemer J

    更新日期:2010-02-26 00:00:00

  • ATXN7L3 and ENY2 Coordinate Activity of Multiple H2B Deubiquitinases Important for Cellular Proliferation and Tumor Growth.

    abstract::Histone H2B monoubiquitination (H2Bub1) is centrally involved in gene regulation. The deubiquitination module (DUBm) of the SAGA complex is a major regulator of global H2Bub1 levels, and components of this DUBm are linked to both neurodegenerative diseases and cancer. Unexpectedly, we find that ablation of USP22, the ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2016.03.030

    authors: Atanassov BS,Mohan RD,Lan X,Kuang X,Lu Y,Lin K,McIvor E,Li W,Zhang Y,Florens L,Byrum SD,Mackintosh SG,Calhoun-Davis T,Koutelou E,Wang L,Tang DG,Tackett AJ,Washburn MP,Workman JL,Dent SY

    更新日期:2016-05-19 00:00:00

  • MEK1 is required for PTEN membrane recruitment, AKT regulation, and the maintenance of peripheral tolerance.

    abstract::The Raf/MEK/ERK and PI3K/Akt pathways are prominent effectors of oncogenic Ras. These pathways negatively regulate each other, but the mechanism involved is incompletely understood. We now identify MEK1 as an essential regulator of lipid/protein phosphatase PTEN, through which it controls phosphatidylinositol-3-phosph...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2013.01.037

    authors: Zmajkovicova K,Jesenberger V,Catalanotti F,Baumgartner C,Reyes G,Baccarini M

    更新日期:2013-04-11 00:00:00

  • An orphan receptor tyrosine kinase family whose members serve as nonintegrin collagen receptors.

    abstract::Mammalian cells constantly monitor and respond to a myriad of extracellular signals, often by using cell surface receptors. Two important classes of cell surface receptors include the receptor tyrosine kinases, which recognize peptide growth factors such as insulin, and the integrins, which most often mediate binding ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(00)80004-0

    authors: Shrivastava A,Radziejewski C,Campbell E,Kovac L,McGlynn M,Ryan TE,Davis S,Goldfarb MP,Glass DJ,Lemke G,Yancopoulos GD

    更新日期:1997-12-01 00:00:00

  • Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli.

    abstract::In Escherichia coli, topoisomerase IV, a type II topoisomerase, mediates the resolution of topological linkages between replicated daughter chromosomes and is essential for chromosome segregation. Topo IV activity is restricted to only a short interval late in the cell cycle. However, the mechanism that confers this t...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2009.01.001

    authors: Madabhushi R,Marians KJ

    更新日期:2009-01-30 00:00:00

  • Achieving fidelity in homologous recombination despite extreme complexity: informed decisions by molecular profiling.

    abstract::In this issue of Molecular Cell, Savir and Tlusty (2010) apply signal detection theory to show that homologous recombination machinery is optimally tuned to find homologous DNA sequences within an exceptionally high background of heterologous sequences. ...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2010.10.032

    authors: Rambo RP,Williams GJ,Tainer JA

    更新日期:2010-11-12 00:00:00

  • The heat shock response: life on the verge of death.

    abstract::Organisms must survive a variety of stressful conditions, including sudden temperature increases that damage important cellular structures and interfere with essential functions. In response to heat stress, cells activate an ancient signaling pathway leading to the transient expression of heat shock or heat stress pro...

    journal_title:Molecular cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.molcel.2010.10.006

    authors: Richter K,Haslbeck M,Buchner J

    更新日期:2010-10-22 00:00:00

  • Bax exists in a dynamic equilibrium between the cytosol and mitochondria to control apoptotic priming.

    abstract::The proapoptotic Bcl-2 protein Bax is predominantly found in the cytosol of nonapoptotic cells and is commonly thought to translocate to mitochondria following an apoptotic stimulus. The current model for Bax activation is that BH3 proteins bind to cytosolic Bax, initiating mitochondrial targeting and outer-membrane p...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2012.12.022

    authors: Schellenberg B,Wang P,Keeble JA,Rodriguez-Enriquez R,Walker S,Owens TW,Foster F,Tanianis-Hughes J,Brennan K,Streuli CH,Gilmore AP

    更新日期:2013-03-07 00:00:00

  • Fifty Shades of α-Ketoglutarate on Cellular Programming.

    abstract::High plasticity to utilize different nutrients to adapt metabolic stress is one of the hallmarks for cancer cells. However, the underlying mechanisms by which cancer cells reprogram metabolic machinery in response to metabolic stress remain largely unclear. In this issue of Molecular Cell, Wang et al. (2019) report th...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2019.09.002

    authors: Di Conza G,Tsai CH,Ho PC

    更新日期:2019-10-03 00:00:00

  • Structural basis for the activation of microtubule assembly by the EB1 and p150Glued complex.

    abstract::Plus-end tracking proteins, such as EB1 and the dynein/dynactin complex, regulate microtubule dynamics. These proteins are thought to stabilize microtubules by forming a plus-end complex at microtubule growing ends with ill-defined mechanisms. Here we report the crystal structure of two plus-end complex components, th...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2005.06.034

    authors: Hayashi I,Wilde A,Mal TK,Ikura M

    更新日期:2005-08-19 00:00:00

  • A branched pathway governing the activation of a developmental transcription factor by regulated intramembrane proteolysis.

    abstract::The proteolytic activation of the membrane-associated transcription factor pro-sigma(K) is controlled by a signal transduction pathway during sporulation in the bacterium Bacillus subtilis. The pro-sigma(K) processing enzyme SpoIVFB, a membrane-embedded metalloprotease, is held inactive by two other integral-membrane ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2006.05.019

    authors: Campo N,Rudner DZ

    更新日期:2006-07-07 00:00:00

  • Yeast Sen1 helicase protects the genome from transcription-associated instability.

    abstract::Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts. We now show that Sen1 helicase possesses a wider function by restricting the occurrence of RNA:DNA hybrids that may naturally form dur...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2010.12.007

    authors: Mischo HE,Gómez-González B,Grzechnik P,Rondón AG,Wei W,Steinmetz L,Aguilera A,Proudfoot NJ

    更新日期:2011-01-07 00:00:00

  • Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation.

    abstract::DNA methylation is an essential epigenetic mark in mammals that has to be re-established after each round of DNA replication. The protein UHRF1 is essential for this process; it has been proposed that the protein targets newly replicated DNA by cooperatively binding hemi-methylated DNA and H3K9me2/3, but this model le...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2017.07.012

    authors: Ferry L,Fournier A,Tsusaka T,Adelmant G,Shimazu T,Matano S,Kirsh O,Amouroux R,Dohmae N,Suzuki T,Filion GJ,Deng W,de Dieuleveult M,Fritsch L,Kudithipudi S,Jeltsch A,Leonhardt H,Hajkova P,Marto JA,Arita K,Shinkai Y

    更新日期:2017-08-17 00:00:00

  • Adaptation in CRISPR-Cas Systems.

    abstract::Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptati...

    journal_title:Molecular cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.molcel.2016.01.030

    authors: Sternberg SH,Richter H,Charpentier E,Qimron U

    更新日期:2016-03-17 00:00:00