Abstract：
:tRNAs reading four-codon families often have a modified uridine, cmo(5)U(34), at the wobble position of the anticodon. Here, we examine the effects on the decoding mechanism of a cmo(5)U modification in tRNA(1B)(Ala), anticodon C(36)G(35)cmo(5)U(34). tRNA(1B)(Ala) reads its cognate codons in a manner that is very similar to that of tRNA(Phe). As Ala codons are GC rich and Phe codons AU rich, this similarity suggests a uniform decoding mechanism that is independent of the GC content of the codon-anticodon duplex or the identity of the tRNA. The presence of cmo(5)U at the wobble position of tRNA(1B)(Ala) permits fairly efficient reading of non-Watson-Crick and nonwobble bases in the third codon position, e.g., the GCC codon. The ribosome accepts the C-cmo(5)U pair as an almost-correct base pair, unlike third-position mismatches, which lead to the incorporation of incorrect amino acids and are efficiently rejected.
journal_name
Mol Celljournal_title
Molecular cellauthors
Kothe U,Rodnina MVdoi
10.1016/j.molcel.2006.11.014subject
Has Abstractpub_date
2007-01-12 00:00:00pages
167-74issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(06)00784-2journal_volume
25pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract：:R loop, a transcription intermediate containing RNA:DNA hybrids and displaced single-stranded DNA (ssDNA), has emerged as a major source of genomic instability. RNaseH1, which cleaves the RNA in RNA:DNA hybrids, plays an important role in R loop suppression. Here we show that replication protein A (RPA), an ssDNA-bind...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2017.01.029
更新日期：2017-03-02 00:00:00
abstract：:The PB1-domain-containing proteins p62, aPKC, MEKK2/MEKK3, MEK5, and Par-6 play roles in critical cell processes like osteoclastogenesis, angiogenesis, and early cardiovascular development or cell polarity. PB1 domains are scaffold modules that adopt the topology of ubiquitin-like beta-grasp folds that interact with e...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2006.08.002
更新日期：2006-09-01 00:00:00
abstract：:Examination of the binding of FeBABE-conjugated BvgA to the fha promoter of Bordetella pertussis has revealed that three dimers, formed by head-to-head association of monomers, bind one face of the DNA helix from the inverted-heptad primary binding site to the -35 region. The orientation of BvgA monomers within the di...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00007-8
更新日期：2003-01-01 00:00:00
abstract：:Cas9 is a prokaryotic RNA-guided DNA endonuclease that binds substrates tightly in vitro but turns over rapidly when used to manipulate genomes in eukaryotic cells. Little is known about the factors responsible for dislodging Cas9 or how they influence genome engineering. Unbiased detection through proximity labeling ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.06.014
更新日期：2020-07-16 00:00:00
abstract：:PARP enzymes are increasingly taking on important roles beyond DNA repair. Huang et al. (2020b) report how the NAD+-dependent ADP-ribosylation of histone H2B by PARP-1 in complex with a metabolic enzyme suppresses the phosphorylation of an adjacent residue, impacting adipogenesis. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2020.08.019
更新日期：2020-09-17 00:00:00
abstract：:HIV-1 Vpu interacts with CD4 in the endoplasmic reticulum and triggers CD4 degradation, presumably by proteasomes. Human beta TrCP identified by interaction with Vpu connects CD4 to this proteolytic machinery, and CD4-Vpu-beta TrCP ternary complexes have been detected by coimmunoprecipitation. beta TrCP binding to Vpu...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80056-8
更新日期：1998-03-01 00:00:00
abstract：:Pre-mRNA 3' end formation is an essential step in eukaryotic gene expression. Over half of human genes produce alternatively polyadenylated mRNAs, suggesting that regulated polyadenylation is an important mechanism for posttranscriptional gene control. Although a number of mammalian mRNA 3' processing factors have bee...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2008.12.028
更新日期：2009-02-13 00:00:00
abstract：:Metformin has been reported to possess antitumor activity and maintain high cytotoxic T lymphocyte (CTL) immune surveillance. However, the functions and detailed mechanisms of metformin's role in cancer immunity are not fully understood. Here, we show that metformin increases CTL activity by reducing the stability and...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.07.030
更新日期：2018-08-16 00:00:00
abstract：:Posttranslational histone modifications play important roles in transcription and other chromatin-based processes. Compared to acetylation, methylation, and phosphorylation, very little is known about the function of histone ubiquitylation. Here, we report the purification and functional characterization of a histone ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.03.035
更新日期：2006-05-05 00:00:00
abstract：:Previous studies suggested that expression of most yeast mRNAs is dominated by either transcription factor TFIID or SAGA. We re-examined the role of TFIID by rapid depletion of S. cerevisiae TFIID subunits and measurement of changes in nascent transcription. We find that transcription of nearly all mRNAs is strongly d...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2017.08.014
更新日期：2017-10-05 00:00:00
abstract：:The positive transcription elongation factor b (P-TEFb) is a cyclin-dependent kinase that controls the elongation phase of transcription by RNA polymerase II (RNAPII). This process is made possible by the reversal of effects of negative elongation factors that include NELF and DSIF. In complex organisms, elongation co...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2006.06.014
更新日期：2006-08-04 00:00:00
abstract：:We have identified ARAP1 and ARAP2 and examined ARAP1 as a possible link between phosphoinositide-, Arf-, and Rho-mediated cell signaling. ARAP1 contains Arf GAP, Rho GAP, Ankyrin repeat, Ras-associating, and five PH domains. In vitro, ARAP1 had Rho GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent A...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00428-8
更新日期：2002-01-01 00:00:00
abstract：:Autophagy constitutes a prominent mechanism through which eukaryotic cells preserve homeostasis in baseline conditions and in response to perturbations of the intracellular or extracellular microenvironment. Autophagic responses can be relatively non-selective or target a specific subcellular compartment. At least in ...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2015.07.021
更新日期：2015-08-20 00:00:00
abstract：:Mitochondrial diseases and aging are associated with defects in the oxidative phosphorylation machinery (OXPHOS), which are the only complexes composed of proteins encoded by separate genomes. To better understand genome coordination and OXPHOS recovery during mitochondrial dysfunction, we examined ATFS-1, a transcrip...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2015.02.008
更新日期：2015-04-02 00:00:00
abstract：:The ER-associated degradation (ERAD) pathway serves as an important cellular safeguard by directing incorrectly folded and unassembled proteins from the ER to the proteasome. Still, however, little is known about the components mediating ERAD of membrane proteins. Here we show that the evolutionary conserved rhomboid ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.06.008
更新日期：2012-08-24 00:00:00
abstract：:Triplex structure-forming GAA/TTC repeats pose a dual threat to the eukaryotic genome integrity. Their potential to expand can lead to gene inactivation, the cause of Friedreich's ataxia disease in humans. In model systems, long GAA/TTC tracts also act as chromosomal fragile sites that can trigger gross chromosomal re...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.08.002
更新日期：2012-10-26 00:00:00
abstract：:Related coactivators p300 and CBP affect the transcriptional activities of many transcription factors (TF), producing multiple downstream effects. Here we show that immediate early response TF, Egr1, acts upstream of p300/CBP to induce or to repress transcription, depending on the stimulus. Cells induced with serum to...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2004.06.030
更新日期：2004-07-02 00:00:00
abstract：:Trimethylation of histone H3 on lysine 4 (H3K4me3) localizes near the 5' region of genes and is tightly associated with active loci. Several proteins, such as CHD1, BPTF, JMJD2A, and the ING tumor suppressor family, directly recognize this lysine methyl mark. However, how H3K4me3 recognition participates in active tra...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.11.010
更新日期：2007-11-30 00:00:00
abstract：:Argonaute proteins are the mediators of small RNA-guided gene silencing pathways. In this issue, Couvillion and coworkers (Couvillion et al., 2012) found an unexpected function for a Tetrahymena Argonaute protein: It forms a complex with tRNA fragments and is required for nuclear Xrn2 localization and function. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2012.11.015
更新日期：2012-11-30 00:00:00
abstract：:Key to the pathogenicity of several viruses is activation of the canonical nuclear factor-kappaB (NF-kappaB) transcriptional pathway. Subversion of this tightly regulated mechanism is achieved through the production of host mimetic viral proteins that deregulate the transcription process. One such protein is ks-vFLIP ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2008.04.029
更新日期：2008-06-06 00:00:00
abstract：:Stress granules and P-bodies are cytosolic biomolecular condensates that dynamically form by the phase separation of RNAs and proteins. They participate in translational control and buffer the proteome. Upon stress, global translation halts and mRNAs bound to the translational machinery and other proteins coalesce to ...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2019.09.014
更新日期：2019-10-17 00:00:00
abstract：:The mechanisms controlling growth and patterning along the proximal-distal axis of the vertebrate limb are yet to be understood. We show that restriction of expression of the homeobox gene Meis2 to proximal regions of the limb bud is essential for limb development, since ectopic Meis2 severely disrupts limb outgrowth....
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80393-7
更新日期：1999-11-01 00:00:00
abstract：:In this issue of Molecular Cell, Schmidt et al. (2009) untangle the interplay between the COP9 signalosome (CSN), cullin-associated and neddylation-dissociated 1 (CAND1) protein, and cullin-RING ubiquitin ligases (CRLs). ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2009.08.011
更新日期：2009-09-11 00:00:00
abstract：:The disulfide relay system in the intermembrane space of mitochondria is of crucial importance for mitochondrial biogenesis. Major players in this pathway are the oxidoreductase Mia40 that oxidizes substrates and the sulfhydryl oxidase Erv1 that reoxidizes Mia40. To analyze in detail the mechanism of this oxidative pa...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.01.017
更新日期：2010-02-26 00:00:00
abstract：:All nuclear transcription is interrupted during mitosis. We examined the role of human TTF2, an RNA polymerase (Pol) I and II termination factor, in mitotic repression of transcription elongation. We find that TTF2 levels rise in the cytoplasm in S and G2 and at the onset of mitosis TTF2 translocates into the nucleus....
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(04)00234-5
更新日期：2004-05-07 00:00:00
abstract：:The fidelity of chromosome segregation depends on the spindle assembly checkpoint (SAC). In the presence of unattached kinetochores, anaphase is delayed when three SAC components (Mad2, Mad3/BubR1, and Bub3) inhibit Cdc20, the activating subunit of the anaphase-promoting complex (APC/C). We analyzed the role of Cdc20 ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.07.031
更新日期：2012-09-28 00:00:00
abstract：:APOBEC1 is the catalytic component of an RNA editing complex but shows homology to activation-induced cytidine deaminase (AID), a protein whose function is to potentiate diversification of immunoglobulin gene DNA. Here, we show that APOBEC1 and its homologs APOBEC3C and APOBEC3G exhibit potent DNA mutator activity in ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00742-6
更新日期：2002-11-01 00:00:00
abstract：:To determine whether replicational mutagenesis in the yeast genome is influenced by the positions of active origins, a reporter gene was placed in two orientations at multiple locations within a 39,000 bp region of chromosome III possessing two strong origins. The frequency of mutations resulting from misincorporation...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00567-1
更新日期：2002-07-01 00:00:00
abstract：:A recent paper by Gallagher et al. (2020) demonstrates that c-di-GMP controls spore formation in Streptomyces venezuelae through sequestering the sporulation sigma factor σWhiG and presents the crystal structure of a ternary complex between c-di-GMP, σWhiG, and its anti-sigma factor, RsiG. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2020.01.018
更新日期：2020-02-06 00:00:00
abstract：:The early detection by the host of invading microorganisms, including viruses, depends on a limited number of specific receptors that recognize pathogen-associated molecular patterns (PAMPs). A few of these PAMPs, including ssRNA and dsRNA, are recognized by Toll-like receptors (TLR)-7/8 and TLR3, respectively. Activa...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2006.05.012
更新日期：2006-06-09 00:00:00
