Abstract：
:Previous studies suggested that expression of most yeast mRNAs is dominated by either transcription factor TFIID or SAGA. We re-examined the role of TFIID by rapid depletion of S. cerevisiae TFIID subunits and measurement of changes in nascent transcription. We find that transcription of nearly all mRNAs is strongly dependent on TFIID function. Degron-dependent depletion of Taf1, Taf2, Taf7, Taf11, and Taf13 showed similar transcription decreases for genes in the Taf1-depleted, Taf1-enriched, TATA-containing, and TATA-less gene classes. The magnitude of TFIID dependence varies with growth conditions, although this variation is similar genome-wide. Many studies have suggested differences in gene-regulatory mechanisms between TATA and TATA-less genes, and these differences have been attributed in part to differential dependence on SAGA or TFIID. Our work indicates that TFIID participates in expression of nearly all yeast mRNAs and that differences in regulation between these two gene categories is due to other properties.
journal_name
Mol Celljournal_title
Molecular cellauthors
Warfield L,Ramachandran S,Baptista T,Devys D,Tora L,Hahn Sdoi
10.1016/j.molcel.2017.08.014subject
Has Abstractpub_date
2017-10-05 00:00:00pages
118-129.e5issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(17)30611-1journal_volume
68pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract：:Networks of protein interactions coordinate cellular functions. We describe a bimolecular fluorescence complementation (BiFC) assay for determination of the locations of protein interactions in living cells. This approach is based on complementation between two nonfluorescent fragments of the yellow fluorescent protei...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00496-3
更新日期：2002-04-01 00:00:00
abstract：:The recent paper by Stadlmann et al. (2017) provides a novel algorithm for glycoproteomics in which complex glycopeptides can be identified in complex mixtures to aid in characterizing both the site of glycosylation and the glycan structure. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2017.11.024
更新日期：2017-12-07 00:00:00
abstract：:Increasing evidence suggests that tRNA levels are dynamically and specifically regulated in response to internal and external cues to modulate the cellular translational program. However, the molecular players and the mechanisms regulating the gene-specific expression of tRNAs are still unknown. Using an inducible aux...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.03.023
更新日期：2020-05-21 00:00:00
abstract：:In this issue, Song et al. (2014) describe how microtubules and the RAN GTPase/importin-β system collaborate to control timing of HURP action and its turnover via the anaphase promoting complex (APC)-proteasome system, thereby ensuring temporal control of late mitotic events. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2014.01.007
更新日期：2014-01-23 00:00:00
abstract：:Expression of BRCA1 is commonly decreased in sporadic breast tumors, and this correlates with poor prognosis of breast cancer patients. Here we show that BRCA1 transcripts are selectively enriched in the Argonaute/miR-182 complex and miR-182 downregulates BRCA1 expression. Antagonizing miR-182 enhances BRCA1 protein l...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.12.005
更新日期：2011-01-21 00:00:00
abstract：:The anaphase-promoting complex/cyclosome (APC/C) regulates sister chromatid segregation and the exit from mitosis. Selection of most APC/C substrates is controlled by coactivator subunits (either Cdc20 or Cdh1) that interact with substrate destruction motifs--predominantly the destruction (D) box and KEN box degrons. ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2013.04.024
更新日期：2013-06-06 00:00:00
abstract：:Epstein-Barr virus (EBV) causes infectious mononucleosis, establishes long-term latent infections, and is associated with a variety of human tumors. The EBV gp42 glycoprotein binds MHC class II molecules, playing a critical role in infection of B lymphocytes. EBV gp42 belongs to the C-type lectin superfamily, with hom...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00465-3
更新日期：2002-02-01 00:00:00
abstract：:One of the prominent biological phenomena controlled by Wnt signaling is the expansion of cells with predefined fates. In the November 27, 2002, issue of Cell, Rosenfeld and colleagues describe an example of Wnt-controlled proliferation during genesis of the cardiac outflow tract and the pituitary gland. As a downstre...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00801-8
更新日期：2002-12-01 00:00:00
abstract：:Productive splicing of human precursor messenger RNAs (pre-mRNAs) requires the correct selection of authentic splice sites (SS) from the large pool of potential SS. Although SS consensus sequence and splicing regulatory proteins are known to influence SS usage, the mechanisms ensuring the effective suppression of cryp...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.08.030
更新日期：2018-11-01 00:00:00
abstract：:Upon glucose restriction, eukaryotic cells upregulate oxidative metabolism to maintain homeostasis. Using genetic screens, we find that the mitochondrial serine hydroxymethyltransferase (SHMT2) is required for robust mitochondrial oxygen consumption and low glucose proliferation. SHMT2 catalyzes the first step in mito...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.01.024
更新日期：2018-02-15 00:00:00
abstract：:Molecular motors are the long-haul carriers of eukaryotic cells, moving cargos bidirectionally along microtubule tracks. In the December 12th issue of Cell, report that HDAC6, a tubulin deacetylase, functions as an adaptor that links cargos of aggregated protein to the minus end-directed motor, cytoplasmic dynein. ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00498-2
更新日期：2003-12-01 00:00:00
abstract：:The Latin word "facultas" literally means "opportunity." Facultative heterochromatin (fHC) then designates genomic regions in the nucleus of a eukaryotic cell that have the opportunity to adopt open or compact conformations within temporal and spatial contexts. This review focuses on the molecular and functional aspec...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2007.09.011
更新日期：2007-10-12 00:00:00
abstract：:The MHC class I antigen presentation system enables T cell immunosurveillance of cancers and viruses. A substantial fraction of the immunopeptidome derives from rapidly degraded nascent polypeptides (DRiPs). By knocking down each of the 80 ribosomal proteins, we identified proteins that modulate peptide generation wit...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.12.020
更新日期：2019-03-21 00:00:00
abstract：:Epigenetic silencing of transposons by Piwi-interacting RNAs (piRNAs) constitutes an RNA-based genome defense mechanism. Piwi endonuclease action amplifies the piRNA pool by generating new piRNAs from target transcripts by a poorly understood mechanism. Here, we identified mouse Fkbp6 as a factor in this biogenesis pa...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.07.019
更新日期：2012-09-28 00:00:00
abstract：:Recent studies, including two in this issue of Molecular Cell (Elbatsh et al., 2016; Beckouët et al., 2016), cast light on how cohesin regulators harness the energy of ATP hydrolysis to open the cohesin ring and enable dynamic, regulated entrapment of chromosomes. ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.02.003
更新日期：2016-02-18 00:00:00
abstract：:The sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner. The PCNA heterotrimer, but not individual subunits, stimulates the activities of the DNA polymerase, DNA ligase I, and the flap endonuclease (FEN1) of S...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00824-9
更新日期：2003-01-01 00:00:00
abstract：:Recycling of RNA polymerase II (Pol II) after transcription requires dephosphorylation of the polymerase C-terminal domain (CTD) by the phosphatase Fcp1. We report the X-ray structure of the small CTD phosphatase Scp1, which is homologous to the Fcp1 catalytic domain. The structure shows a core fold and an active cent...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2004.06.035
更新日期：2004-08-13 00:00:00
abstract：:How proteasomal ATPases stimulate the gate opening of 20S proteasomes is a longstanding question. In the September 7 issue of Molecular Cell, Smith et al. (2007) describe the conserved "HbYX" motif as the key to proteasome gate opening. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2007.09.001
更新日期：2007-09-21 00:00:00
abstract：:Metformin has been reported to possess antitumor activity and maintain high cytotoxic T lymphocyte (CTL) immune surveillance. However, the functions and detailed mechanisms of metformin's role in cancer immunity are not fully understood. Here, we show that metformin increases CTL activity by reducing the stability and...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.07.030
更新日期：2018-08-16 00:00:00
abstract：:In this issue of Molecular Cell, Hogues et al. (2008) demonstrate a wholesale shift in the key regulatory protein involved in ribosomal protein (RP) synthesis during the evolution of S. cerevisiae and, en passant, raise interesting questions about the relationship between RP genes and telomeres. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2008.02.008
更新日期：2008-03-14 00:00:00
abstract：:In this issue of Molecular Cell, Yang et al. (2011) demonstrate that Aurora B phosphorylates ATM, leading to its mitotic activation and ability to phosphorylate Bub1 and regulate the spindle checkpoint, thus maintaining genomic integrity. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2011.11.001
更新日期：2011-11-18 00:00:00
abstract：:K-Ras associates with the plasma membrane (PM) through farnesylation that functions in conjunction with an adjacent polybasic sequence. We show that phosphorylation by protein kinase C (PKC) of S181 within the polybasic region promotes rapid dissociation of K-Ras from the PM and association with intracellular membrane...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.01.012
更新日期：2006-02-17 00:00:00
abstract：:The bHLH factors HAND1 and HAND2 are required for heart, vascular, neuronal, limb, and extraembryonic development. Unlike most bHLH proteins, HAND factors exhibit promiscuous dimerization properties. We report that phosphorylation/dephosphorylation via PKA, PKC, and a specific heterotrimeric protein phosphatase 2A (PP...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00425-8
更新日期：2003-11-01 00:00:00
abstract：:Bacterial actin MreB is one of the key components of the bacterial cytoskeleton. It assembles into short filaments that lie just underneath the membrane and organize the cell wall synthesis machinery. Here we show that MreB from both T. maritima and E. coli binds directly to cell membranes. This function is essential ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.07.008
更新日期：2011-08-05 00:00:00
abstract：:RNA flow between organisms has been documented within and among different kingdoms of life. Recently, we demonstrated horizontal RNA transfer between honeybees involving secretion and ingestion of worker and royal jellies. However, how the jelly facilitates transfer of RNA is still unknown. Here, we show that worker a...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.03.010
更新日期：2019-05-02 00:00:00
abstract：:In this issue, Roberts et al. (2014) describe how hexokinase and mTORC1 cooperate to sense disequilibrium between glucose uptake and utilization and direct the balance of anabolism and catabolism to ensure the appropriate use of cellular resources. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2014.02.009
更新日期：2014-02-20 00:00:00
abstract：:Trimethylation of histone H3 on lysine 4 (H3K4me3) localizes near the 5' region of genes and is tightly associated with active loci. Several proteins, such as CHD1, BPTF, JMJD2A, and the ING tumor suppressor family, directly recognize this lysine methyl mark. However, how H3K4me3 recognition participates in active tra...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.11.010
更新日期：2007-11-30 00:00:00
abstract：:GATA-1 and GATA-2 control proliferation and differentiation of hematopoietic progenitor cells via transcriptional regulation. In this issue of Molecular Cell, Jing et al. (2008) demonstrate that GATA factor exchange on the Kit locus directs a transcriptional switch by reconfiguring chromatin loops. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2008.01.008
更新日期：2008-02-01 00:00:00
abstract：:Faithful duplication of the genome in S phase followed by its accurate segregation in mitosis is essential to maintain genomic integrity. Recent studies have suggested that proteins involved in DNA transactions are also required for whole-chromosome stability. Here we demonstrate that the MRN (Mre11, Rad50, and Nbs1) ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2013.01.023
更新日期：2013-03-28 00:00:00
abstract：:RING ubiquitin ligases (E3) recruit ubiquitin-conjugate enzymes (E2) charged with ubiquitin (Ub) to catalyze ubiquitination. Non-covalent Ub binding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains elusive. Here, we show that backside bound Ub (Ub(B)) enhances both RING-in...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2015.02.017
更新日期：2015-04-16 00:00:00
