Abstract：
:Recycling of RNA polymerase II (Pol II) after transcription requires dephosphorylation of the polymerase C-terminal domain (CTD) by the phosphatase Fcp1. We report the X-ray structure of the small CTD phosphatase Scp1, which is homologous to the Fcp1 catalytic domain. The structure shows a core fold and an active center similar to those of phosphotransferases and phosphohydrolases that solely share a DXDX(V/T) signature motif with Fcp1/Scp1. We demonstrate that the first aspartate in the signature motif undergoes metal-assisted phosphorylation during catalysis, resulting in a phosphoaspartate intermediate that was structurally mimicked with the inhibitor beryllofluoride. Specificity may result from CTD binding to a conserved hydrophobic pocket between the active site and an insertion domain that is unique to Fcp1/Scp1. Fcp1 specificity may additionally arise from phosphatase recruitment near the CTD via the Pol II subcomplex Rpb4/7, which is shown to be required for binding of Fcp1 to the polymerase in vitro.
journal_name
Mol Celljournal_title
Molecular cellauthors
Kamenski T,Heilmeier S,Meinhart A,Cramer Pdoi
10.1016/j.molcel.2004.06.035subject
Has Abstractpub_date
2004-08-13 00:00:00pages
399-407issue
3eissn
1097-2765issn
1097-4164pii
S1097-2765(04)00380-6journal_volume
15pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract：:While much research has examined the use of glucose and glutamine by tumor cells, many cancers instead prefer to metabolize fats. Despite the pervasiveness of this phenotype, knowledge of pathways that drive fatty acid oxidation (FAO) in cancer is limited. Prolyl hydroxylase domain proteins hydroxylate substrate proli...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.08.014
更新日期：2016-09-15 00:00:00
abstract：:APOBEC1 is the catalytic component of an RNA editing complex but shows homology to activation-induced cytidine deaminase (AID), a protein whose function is to potentiate diversification of immunoglobulin gene DNA. Here, we show that APOBEC1 and its homologs APOBEC3C and APOBEC3G exhibit potent DNA mutator activity in ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00742-6
更新日期：2002-11-01 00:00:00
abstract：:A defining feature of centromeres is the presence of the histone H3 variant CENP-A(Cnp1). It is not known how CENP-A(Cnp1) is specifically delivered to, and assembled into, centromeric chromatin. Through a screen for factors involved in kinetochore integrity in fission yeast, we identified Sim3. Sim3 is homologous to ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.10.010
更新日期：2007-12-28 00:00:00
abstract：:In addition to its role as an electron transporter, mitochondrial nicotinamide adenine dinucleotide (NAD+) is an important co-factor for enzymatic reactions, including ADP-ribosylation. Although mitochondria harbor the most intra-cellular NAD+, mitochondrial ADP-ribosylation remains poorly understood. Here we provide ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.12.034
更新日期：2021-01-21 00:00:00
abstract：:In this issue of Molecular Cell, Chen et al. (2014) provide evidence that FMRP represses translation by binding the ribosome, suggesting a novel form of translational control. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2014.04.027
更新日期：2014-05-08 00:00:00
abstract：:In this issue of Molecular Cell, Chereji et al. (2017) present new data on MNase-sensitive particles previously identified upstream of transcription start sites at many promoters in budding yeast, and they argue, based upon negative histone-ChIP results, that they are non-nucleosomal signals generated by transcription...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2017.01.010
更新日期：2017-02-02 00:00:00
abstract：:Mammalian lefty and zebrafish antivin form a subgroup of the TGF beta superfamily. We report that mouse mutants for lefty2 have an expanded primitive streak and form excess mesoderm, a phenotype opposite to that of mutants for the TGF beta gene nodal. Analogously, overexpression of Antivin or Lefty2 in zebrafish embry...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80331-7
更新日期：1999-09-01 00:00:00
abstract：:Prenylated Rab proteins exist in the cytosol as soluble, high-affinity complexes with GDI that need to be disrupted for membrane attachment and targeting of Rab proteins. The Legionella pneumophila protein DrrA displaces GDI from Rab1:GDI complexes, incorporating Rab1 into Legionella-containing vacuoles and activating...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.11.014
更新日期：2009-12-25 00:00:00
abstract：:Ubiquitin (UB)-driven signaling systems permeate biology, and are often integrated with other types of post-translational modifications (PTMs), including phosphorylation. Flux through such pathways is dictated by the fractional stoichiometry of distinct modifications and protein assemblies as well as the spatial organ...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2015.02.020
更新日期：2015-05-21 00:00:00
abstract：:The fidelity of chromosome segregation depends on the spindle assembly checkpoint (SAC). In the presence of unattached kinetochores, anaphase is delayed when three SAC components (Mad2, Mad3/BubR1, and Bub3) inhibit Cdc20, the activating subunit of the anaphase-promoting complex (APC/C). We analyzed the role of Cdc20 ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.07.031
更新日期：2012-09-28 00:00:00
abstract：:Multisite phosphorylation of kinases can induce on-off or graded regulation of catalytic activity; however, its influence on substrate specificity remains unclear. Here, we show that multisite phosphorylation of ribosomal protein S6 kinase 1 (S6K1) alters target selection. Agonist-inducible phosphorylation of glutamyl...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.11.017
更新日期：2019-02-07 00:00:00
abstract：:Perfringolysin O, a bacterial cytolytic toxin, forms unusually large pores in cholesterol-containing membranes by the spontaneous insertion of two of its four domains into the bilayer. By monitoring the kinetics of domain-specific conformational changes and pore formation using fluorescence spectroscopy, the temporal ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)00119-2
更新日期：2000-11-01 00:00:00
abstract：:The eukaryotic RNA exosome is an essential and conserved 3'-to-5' exoribonuclease complex that degrades or processes nearly every class of cellular RNA. The nuclear RNA exosome includes a 9-subunit non-catalytic core that binds Rrp44 (Dis3) and Rrp6 subunits to modulate their processive and distributive 3'-to-5' exori...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.09.038
更新日期：2016-11-17 00:00:00
abstract：:In this issue of Molecular Cell, Hogues et al. (2008) demonstrate a wholesale shift in the key regulatory protein involved in ribosomal protein (RP) synthesis during the evolution of S. cerevisiae and, en passant, raise interesting questions about the relationship between RP genes and telomeres. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2008.02.008
更新日期：2008-03-14 00:00:00
abstract：:DNA double-strand breaks (DSBs) arise during physiological transcription, DNA replication, and antigen receptor diversification. Mistargeting or misprocessing of DSBs can result in pathological structural variation and mutation. Here we describe a sensitive method (END-seq) to monitor DNA end resection and DSBs genome...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.06.034
更新日期：2016-09-01 00:00:00
abstract：:Many genomic studies have revealed associations between the gut microbiota composition and host metabolism. These observations led to the idea that a causal relationship could exist between the microbiota and metabolic diseases, a concept supported by studies showing compositional changes in the microbial community in...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2020.03.005
更新日期：2020-05-21 00:00:00
abstract：:Transcriptional coregulators, rather than ligand signals, are suspected to confer context and pathway specificity to nuclear receptor signaling, but the identity of such specifying coregulators and the underlying molecular mechanisms remain largely enigmatic. Here we address this issue in metabolic oxysterol receptor ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.05.006
更新日期：2009-05-14 00:00:00
abstract：:We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-beta-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, inc...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.06.021
更新日期：2010-07-30 00:00:00
abstract：:Nucleosomes are surprisingly dynamic structures in vivo, showing transcription-independent exchange of histones H2A-H2B genome-wide and exchange of H3-H4 mainly within the promoters of transcribed genes. In addition, nucleosomes are disrupted in front of and reassembled behind the elongating RNA polymerase. Here we sh...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.07.001
更新日期：2009-08-14 00:00:00
abstract：:In eukaryotes, a crucial step of translation initiation is the binding of the multifactor complex eIF4F to the 5' end of the mRNA, a prerequisite to recruitment of the activated small ribosomal 43S particle. Histone H4 mRNAs have short 5'UTRs, which do not conform to the conventional scanning-initiation model. Here we...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.12.019
更新日期：2011-01-21 00:00:00
abstract：:All nuclear transcription is interrupted during mitosis. We examined the role of human TTF2, an RNA polymerase (Pol) I and II termination factor, in mitotic repression of transcription elongation. We find that TTF2 levels rise in the cytoplasm in S and G2 and at the onset of mitosis TTF2 translocates into the nucleus....
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(04)00234-5
更新日期：2004-05-07 00:00:00
abstract：:Serine/arginine (SR)-rich splicing factors contain an RNA binding domain and an arginine/serine (RS)-rich domain required for protein-protein interactions. In addition to their roles in the basic splicing reaction, SR proteins function as components of splicing enhancer complexes. Here, we investigate the role of RS d...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80076-3
更新日期：1998-04-01 00:00:00
abstract：:DNA double-strand break (DSB) signaling and repair are critical for cell viability, and rely on highly coordinated pathways whose molecular organization is still incompletely understood. Here, we show that heterogeneous nuclear ribonucleoprotein U-like (hnRNPUL) proteins 1 and 2 play key roles in cellular responses to...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.12.035
更新日期：2012-02-24 00:00:00
abstract：:The eukaryotic RNA polymerases Pol I, II, and III use different promoters to transcribe different classes of genes. Promoter usage relies on initiation factors, including TFIIF and TFIIE, in the case of Pol II. Here, we show that the Pol I-specific subunits A49 and A34.5 form a subcomplex that binds DNA and is related...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.07.028
更新日期：2010-08-27 00:00:00
abstract：:Certain nascent peptide sequences, when within the ribosomal exit tunnel, can inhibit translation termination and/or peptide elongation. The 24 residue leader peptidyl-tRNA of the tna operon of E. coli, TnaC-tRNA(Pro), in the presence of excess tryptophan, resists cleavage at the tnaC stop codon. TnaC residue Trp12 is...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.06.013
更新日期：2005-08-05 00:00:00
abstract：:The splitting of chromosomes in anaphase and their delivery into the daughter cells needs to be accurately executed to maintain genome stability. Chromosome splitting requires the degradation of securin, whereas the distribution of the chromosomes into the daughter cells requires the degradation of cyclin B. We show t...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2015.09.022
更新日期：2015-11-05 00:00:00
abstract：:The intracellular regulation of cell death pathways by cIAPs has been enigmatic. Here we show that loss of cIAPs promotes the spontaneous formation of an intracellular platform that activates either apoptosis or necroptosis. This 2 MDa intracellular complex that we designate "Ripoptosome" is necessary but not sufficie...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.06.011
更新日期：2011-08-05 00:00:00
abstract：:Peptide release on the ribosome is catalyzed in the large subunit peptidyl transferase center by release factors on recognition of stop codons in the small subunit decoding center. Here we examine the role of the decoding center in this process. Mutation of decoding center nucleotides or removal of 2'OH groups from th...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.09.015
更新日期：2007-11-30 00:00:00
abstract：:Group II intron RNPs are mobile genetic elements that attack and invade duplex DNA. In this work, we monitor the invasion reaction in vitro and establish a quantitative kinetic framework for the steps of this complex cascade. We find that target site specificity is achieved after DNA binding, which occurs nonspecifica...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00069-8
更新日期：2003-03-01 00:00:00
abstract：:Nup98 is a component of the nuclear pore that plays its primary role in the export of RNAs. Nup98 is expressed in two forms, derived from alternate mRNA splicing. Both forms are processed into two peptides through autoproteolysis mediated by the C-terminal domain of hNup98. The three-dimensional structure of the C-ter...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00589-0
更新日期：2002-08-01 00:00:00
