Abstract：
:The anaphase-promoting complex/cyclosome (APC/C) regulates sister chromatid segregation and the exit from mitosis. Selection of most APC/C substrates is controlled by coactivator subunits (either Cdc20 or Cdh1) that interact with substrate destruction motifs--predominantly the destruction (D) box and KEN box degrons. How coactivators recognize D box degrons and how this is inhibited by APC/C regulatory proteins is not defined at the atomic level. Here, from the crystal structure of S. cerevisiae Cdh1 in complex with its specific inhibitor Acm1, which incorporates D and KEN box pseudosubstrate motifs, we describe the molecular basis for D box recognition. Additional interactions between Acm1 and Cdh1 identify a third protein-binding site on Cdh1 that is likely to confer coactivator-specific protein functions including substrate association. We provide a structural rationalization for D box and KEN box recognition by coactivators and demonstrate that many noncanonical APC/C degrons bind APC/C coactivators at the D box coreceptor.
journal_name
Mol Celljournal_title
Molecular cellauthors
He J,Chao WC,Zhang Z,Yang J,Cronin N,Barford Ddoi
10.1016/j.molcel.2013.04.024subject
Has Abstractpub_date
2013-06-06 00:00:00pages
649-60issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(13)00329-8journal_volume
50pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract：:Cardiac disease remains the leading cause of morbidity and mortality worldwide. The β1-adrenergic receptor (β1-AR) is a major regulator of cardiac functions and is downregulated in the majority of heart failure cases. A key physiological process is the activation of heterotrimeric G-protein Gs by β1-ARs, leading to in...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.08.001
更新日期：2020-10-01 00:00:00
abstract：:DNA lesions caused by UV damage are thought to be repaired solely by the nucleotide excision repair (NER) pathway in human cells. Patients carrying mutations within genes functioning in this pathway display a range of pathologies, including an increased susceptibility to cancer, premature aging, and neurological defec...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2017.10.021
更新日期：2017-11-16 00:00:00
abstract：:Mutation and prevalence of pathogenic viruses prompt the development of broad-spectrum antiviral strategies. Viperin is a potent antiviral protein that inhibits a broad range of viruses. Unexpectedly, we found that Viperin protein production in epithelium is defective in response to both viruses and interferons (IFNs)...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.11.003
更新日期：2020-02-20 00:00:00
abstract：:To unravel the roles of LXRs in inflammation and immunity, we examined the function of LXRs in development of IFN-gamma-mediated inflammation using cultured rat brain astrocytes. LXR ligands inhibit neither STAT1 phosphorylation nor STAT1 translocation to the nucleus but, rather, inhibit STAT1 binding to promoters and...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.07.021
更新日期：2009-09-24 00:00:00
abstract：:Stress granules (SGs) are cytoplasmic assemblies of mRNPs stalled in translation initiation. They are induced by various stress conditions, including exposure to the environmental toxin and carcinogen arsenic. While perturbed SG turnover is linked to the pathogenesis of neurodegenerative diseases, the molecular mechan...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.04.021
更新日期：2018-06-07 00:00:00
abstract：:Ataxia Telangiectasia Mutated (ATM) signaling is essential for the repair of a subset of DNA double-strand breaks (DSBs); however, its precise role is unclear. Here, we show that < or =25% of DSBs require ATM signaling for repair, and this percentage correlates with increased chromatin but not damage complexity. Impor...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2008.05.017
更新日期：2008-07-25 00:00:00
abstract：:The ER-mitochondrial junction provides a local calcium signaling domain that is critical for both matching energy production with demand and the control of apoptosis. Here, we visualize ER-mitochondrial contact sites and monitor the localized [Ca(2+)] changes ([Ca(2+)](ER-mt)) using drug-inducible fluorescent interorg...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.06.029
更新日期：2010-07-09 00:00:00
abstract：:Androgen receptor (AR) plays a critical role in the development and progression of prostate cancer, where it is a key therapeutic target. Here we report that, in contrast to estrogen receptor transcription complexes which form within minutes and recycle hourly, the levels of regulatory regions bound by AR complexes ri...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.07.018
更新日期：2005-09-02 00:00:00
abstract：:Ubiquitination typically occurs through the sequential action of three enzymes catalyzing ubiquitin activation (E1), conjugation (E2), and ligation (E3) and regulates diverse eukaryotic cellular processes. Although monoubiquitination commonly confers nondegradative activities, mechanisms underlying its temporal and sp...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.04.022
更新日期：2016-06-16 00:00:00
abstract：:Takahashi et al. (2018) report that the peptide CLE25 together with the BAM1, BAM3 LRR receptor-like kinases are involved in root-to-shoot communication during dehydration stress in Arabidopsis. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2018.06.011
更新日期：2018-06-21 00:00:00
abstract：:YAP/TEAD are nuclear effectors of the Hippo pathway, regulating organ size and tumorigenesis largely through promoter-associated function. However, their function as enhancer regulators remains poorly understood. Through an in vivo proximity-dependent labeling (BioID) technique, we identified YAP1 and TEAD4 protein as...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.06.010
更新日期：2019-08-22 00:00:00
abstract：:Prions constitute a rare class of protein, which can switch to a robust amyloid form and then propagate that form in the absence of a nucleic acid determinant, thereby creating a unique, protein-only infectious agent. Details of the mechanism that drives conversion to the prion form and then subsequent propagation of ...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2004.05.012
更新日期：2004-06-04 00:00:00
abstract：:In this issue of Molecular Cell, Guo et al. (2014) report that misfolded or aggregated nuclear proteins, such as pathogenic polyQ proteins, are cleared by a SUMO-dependent quality control pathway, which involves the E3 SUMO ligase PML and the SUMO-targeted ubiquitin ligase RNF4. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2014.06.022
更新日期：2014-07-03 00:00:00
abstract：:In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic macromolecular machine. U4/U6.U5 tri-snRNP is the largest and most conserved building block of the spliceosome. By 3D elect...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.08.021
更新日期：2006-10-20 00:00:00
abstract：:We have identified human MBT domain-containing protein L3MBTL2 as an integral component of a protein complex that we termed Polycomb repressive complex 1 (PRC1)-like 4 (PRC1L4), given the copresence of PcG proteins RING1, RING2, and PCGF6/MBLR. PRC1L4 also contained E2F6 and CBX3/HP1γ, known to function in transcripti...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.04.004
更新日期：2011-05-20 00:00:00
abstract：:Argonaute proteins are the mediators of small RNA-guided gene silencing pathways. In this issue, Couvillion and coworkers (Couvillion et al., 2012) found an unexpected function for a Tetrahymena Argonaute protein: It forms a complex with tRNA fragments and is required for nuclear Xrn2 localization and function. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2012.11.015
更新日期：2012-11-30 00:00:00
abstract：:Progressive phosphorylation of circadian clock proteins is a hallmark of time-keeping. In this issue of Molecular Cell, Querfurth et al. (2011) demonstrate that phosphorylation of Neurospora FRQ induces a conformational change, which can account for its temporally gated degradation. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2011.08.015
更新日期：2011-09-02 00:00:00
abstract：:In bacterial and archaeal CRISPR immune pathways, DNA sequences from invading bacteriophage or plasmids are integrated into CRISPR loci within the host genome, conferring immunity against subsequent infections. The ribonucleoprotein complex Cascade utilizes RNAs generated from these loci to target complementary "nonse...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2012.03.020
更新日期：2012-06-08 00:00:00
abstract：:The Chk2 Ser/Thr kinase plays crucial, evolutionarily conserved roles in cellular responses to DNA damage. Identification of two pro-oncogenic mutations within the Chk2 FHA domain has highlighted its importance for Chk2 function in checkpoint activation. The X-ray structure of the Chk2 FHA domain in complex with an in...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00527-0
更新日期：2002-05-01 00:00:00
abstract：:The transcriptional activity of many sequence-specific DNA binding proteins is directly regulated by posttranslational covalent modification. Although this form of regulation was first described nearly two decades ago, it remains poorly understood at a mechanistic level. The prototype for a transcription factor contro...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.08.013
更新日期：2005-10-07 00:00:00
abstract：:R-loop, a three-stranded RNA/DNA structure, has been linked to induced genome instability and regulated gene expression. To enable precision analysis of R-loops in vivo, we develop an RNase-H-based approach; this reveals predominant R-loop formation near gene promoters with strong G/C skew and propensity to form G-qua...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2017.10.008
更新日期：2017-11-16 00:00:00
abstract：:Formation and maintenance of specialized plasma membrane domains are crucial for many biological processes, such as cell polarization and signaling. During isotropic bud growth, the yeast cell periphery is divided into two domains: the bud surface, an active site of exocytosis and growth, and the relatively quiescent ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80324-x
更新日期：2000-05-01 00:00:00
abstract：:Signals transmitted by ERK MAP kinases regulate the functions of multiple substrates present in the nucleus and in the cytoplasm. ERK signals are optimized by scaffold proteins that modulate their intensity and spatial fidelity. Once phosphorylated, ERKs dimerize, but how dimerization impacts on the activation of the ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2008.07.024
更新日期：2008-09-05 00:00:00
abstract：:Glycogen is the major mammalian glucose storage cache and is critical for energy homeostasis. Glycogen synthesis in neurons must be tightly controlled due to neuronal sensitivity to perturbations in glycogen metabolism. Lafora disease (LD) is a fatal, congenital, neurodegenerative epilepsy. Mutations in the gene encod...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2014.11.020
更新日期：2015-01-22 00:00:00
abstract：:DnaA occupies only the three highest-affinity binding sites in E. coli oriC throughout most of the cell cycle. Immediately prior to initiation of chromosome replication, DnaA interacts with additional recognition sites, resulting in localized DNA-strand separation. These two DnaA-oriC complexes formed during the cell ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.09.016
更新日期：2006-11-17 00:00:00
abstract：:In this issue of Molecular Cell, Lim et al. (2010) show that SIRT1 deacetylates HIF-1alpha and regulates its ability to respond to hypoxia, revealing yet another important function of SIRT1 and suggesting a connection between HIF function in aging and sirtuin enzymes. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2010.06.015
更新日期：2010-06-25 00:00:00
abstract：:In this issue of Molecular Cell, Rinaldi et al. (2021) reveal how nutrient availability in the metastatic niche of breast cancer cells shapes their growth signaling, highlighting potential avenues for therapeutic interventions. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2020.12.044
更新日期：2021-01-21 00:00:00
abstract：:SOCS3 is essential for regulating the extent, duration, and specificity of cellular responses to cytokines such as G-CSF and IL-6. Here we describe the solution structure of SOCS3, the first structure determined for any SOCS protein, in complex with a phosphotyrosine-containing peptide from the IL-6 receptor signaling...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.03.024
更新日期：2006-04-21 00:00:00
abstract：:MazF and MazE are components of a chromosomal toxin-antitoxin system of Escherichia coli. In this issue of Molecular Cell, Kamada et al. describe the crystal structure of a MazE/MazF heterohexamer and propose that the mechanism of toxin-antidote recognition is common to other homologous chromosomal and plasmid-borne s...
journal_title：Molecular cell
pub_type： 评论,杂志文章,评审
doi：10.1016/s1097-2765(03)00146-1
更新日期：2003-04-01 00:00:00
abstract：:Rho factor in bacteria terminates transcription by using energy from ATP hydrolysis to forcefully dissociate the transcripts from RNA polymerase. used data from presteady-state ATPase kinetics to support a rational mechanistic model for Rho's action on RNA. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.molcel.2006.06.002
更新日期：2006-06-23 00:00:00
