Abstract:
:Glycogen is the major mammalian glucose storage cache and is critical for energy homeostasis. Glycogen synthesis in neurons must be tightly controlled due to neuronal sensitivity to perturbations in glycogen metabolism. Lafora disease (LD) is a fatal, congenital, neurodegenerative epilepsy. Mutations in the gene encoding the glycogen phosphatase laforin result in hyperphosphorylated glycogen that forms water-insoluble inclusions called Lafora bodies (LBs). LBs induce neuronal apoptosis and are the causative agent of LD. The mechanism of glycogen dephosphorylation by laforin and dysfunction in LD is unknown. We report the crystal structure of laforin bound to phosphoglucan product, revealing its unique integrated tertiary and quaternary structure. Structure-guided mutagenesis combined with biophysical and biochemical analyses reveal the basis for normal function of laforin in glycogen metabolism. Analyses of LD patient mutations define the mechanism by which subsets of mutations disrupt laforin function. These data provide fundamental insights connecting glycogen metabolism to neurodegenerative disease.
journal_name
Mol Celljournal_title
Molecular cellauthors
Raththagala M,Brewer MK,Parker MW,Sherwood AR,Wong BK,Hsu S,Bridges TM,Paasch BC,Hellman LM,Husodo S,Meekins DA,Taylor AO,Turner BD,Auger KD,Dukhande VV,Chakravarthy S,Sanz P,Woods VL Jr,Li S,Vander Kooi CW,Gentrydoi
10.1016/j.molcel.2014.11.020subject
Has Abstractpub_date
2015-01-22 00:00:00pages
261-72issue
2eissn
1097-2765issn
1097-4164pii
S1097-2765(14)00915-0journal_volume
57pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract::The Polycomb repressive complex 2 (PRC2) confers transcriptional repression through histone H3 lysine 27 trimethylation (H3K27me3). Here, we examined how PRC2 is modulated by histone modifications associated with transcriptionally active chromatin. We provide the molecular basis of histone H3 N terminus recognition by...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.03.025
更新日期:2011-05-06 00:00:00
abstract::Spt6 is an essential histone chaperone that mediates nucleosome reassembly during gene transcription. Spt6 also associates with RNA polymerase II (RNAPII) via a tandem Src2 homology domain. However, the significance of Spt6-RNAPII interaction is not well understood. Here, we show that Spt6 recruitment to genes and the...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.05.020
更新日期:2018-06-21 00:00:00
abstract::HIV-1 Vpu interacts with CD4 in the endoplasmic reticulum and triggers CD4 degradation, presumably by proteasomes. Human beta TrCP identified by interaction with Vpu connects CD4 to this proteolytic machinery, and CD4-Vpu-beta TrCP ternary complexes have been detected by coimmunoprecipitation. beta TrCP binding to Vpu...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80056-8
更新日期:1998-03-01 00:00:00
abstract::Prenylated Rab proteins exist in the cytosol as soluble, high-affinity complexes with GDI that need to be disrupted for membrane attachment and targeting of Rab proteins. The Legionella pneumophila protein DrrA displaces GDI from Rab1:GDI complexes, incorporating Rab1 into Legionella-containing vacuoles and activating...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2009.11.014
更新日期:2009-12-25 00:00:00
abstract::By using single-molecule fluorescence resonance energy transfer (smFRET), we observe the real-time dynamic coupling between the ribosome, labeled at the L1 stalk, and transfer RNA (tRNA). We find that an interaction between the ribosomal L1 stalk and the newly deacylated tRNA is established spontaneously upon peptide ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2008.03.012
更新日期:2008-05-09 00:00:00
abstract::Aberrant folding and fibrillar aggregation by polyglutamine (polyQ) expansion proteins are associated with cytotoxicity in Huntington's disease and other neurodegenerative disorders. Hsp70 chaperones have an inhibitory effect on fibril formation and can alleviate polyQ cytotoxicity. Here we show that the cytosolic cha...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.08.017
更新日期:2006-09-15 00:00:00
abstract::R-loop, a three-stranded RNA/DNA structure, has been linked to induced genome instability and regulated gene expression. To enable precision analysis of R-loops in vivo, we develop an RNase-H-based approach; this reveals predominant R-loop formation near gene promoters with strong G/C skew and propensity to form G-qua...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.10.008
更新日期:2017-11-16 00:00:00
abstract::Epstein-Barr virus (EBV) causes infectious mononucleosis, establishes long-term latent infections, and is associated with a variety of human tumors. The EBV gp42 glycoprotein binds MHC class II molecules, playing a critical role in infection of B lymphocytes. EBV gp42 belongs to the C-type lectin superfamily, with hom...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00465-3
更新日期:2002-02-01 00:00:00
abstract::During X-inactivation, Xist RNA spreads along an entire chromosome to establish silencing. However, the mechanism and functional RNA elements involved in spreading remain undefined. By performing a comprehensive endogenous Xist deletion screen, we identify Repeat B as crucial for spreading Xist and maintaining Polycom...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2019.01.015
更新日期:2019-04-04 00:00:00
abstract::Anti-sense transcription originating upstream of mammalian protein-coding genes is a well-documented phenomenon, but remarkably little is known about the regulation or function of anti-sense promoters and the non-coding RNAs they generate. Here we define at nucleotide resolution the divergent transcription start sites...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2015.04.006
更新日期:2015-06-18 00:00:00
abstract::Transcriptional and posttranslational signals are known mechanisms that promote efficient responses to DNA damage. We have identified Saccharomyces cerevisiae tRNA methyltransferase 9 (Trm9) as an enzyme that prevents cell death via translational enhancement of DNA damage response proteins. Trm9 methylates the uridine...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.09.021
更新日期:2007-12-14 00:00:00
abstract::Progressive phosphorylation of circadian clock proteins is a hallmark of time-keeping. In this issue of Molecular Cell, Querfurth et al. (2011) demonstrate that phosphorylation of Neurospora FRQ induces a conformational change, which can account for its temporally gated degradation. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2011.08.015
更新日期:2011-09-02 00:00:00
abstract::The heat shock protein 90 (Hsp90) family of heat shock proteins is an abundantly expressed and highly conserved family of ATP-dependent molecular chaperones. Hsp90 facilitates remodeling and activation of hundreds of proteins. In this study, we developed a screen to identify Hsp90-defective mutants in E. coli. The mut...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2012.11.017
更新日期:2013-02-07 00:00:00
abstract::Mirtrons are alternative precursors for microRNA biogenesis that were recently described in invertebrates. These short hairpin introns use splicing to bypass Drosha cleavage, which is otherwise essential for the generation of canonical animal microRNAs. Using computational and experimental strategies, we now establish...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.09.028
更新日期:2007-10-26 00:00:00
abstract::In this issue, Randow and coworkers (von Muhlinen et al., 2012) report that LC3C plays an essential role in Salmonella clearance by interacting with the autophagic receptor NDP52, with consequent recruitment of all other ATG8 proteins to assist in the buildup of the autophagic membrane. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2012.10.020
更新日期:2012-11-09 00:00:00
abstract::Target site choice is a complex and poorly understood aspect of DNA transposition despite its importance in rational transposon-mediated gene delivery. Though most transposons choose target sites essentially randomly or with some slight sequence or structural preferences, insertion sequence IS608 from Helicobacter pyl...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2009.05.017
更新日期:2009-06-12 00:00:00
abstract::Polyubiquitin chains of different topologies regulate diverse cellular processes. K48- and K63-linked chains, the two most abundant chain types, regulate proteolytic and signaling pathways, respectively. Although recent studies reported important roles for heterogeneous chains, the functions of branched ubiquitin chai...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.09.014
更新日期:2016-10-20 00:00:00
abstract::In order to determine the global effects of oncogenic Ras and Akt signaling pathways on translational efficiencies, we compared the gene expression profiles of total cellular mRNA and mRNA associated with polysomes. We found that the immediate effect of Ras and Akt signaling blockade on transcription was relatively mo...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00395-2
更新日期:2003-10-01 00:00:00
abstract::Messenger RNAs with premature translation termination codons (PTCs) are degraded by nonsense-mediated mRNA decay (NMD). In mammals, PTCs are discriminated from physiological stop codons by a process thought to involve the splicing-dependent deposition of an exon junction complex (EJC), EJC-mediated recruitment of Upf3...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00142-4
更新日期:2003-04-01 00:00:00
abstract::The WTX gene is frequently lost or mutated in Wilms tumor. In this issue of Molecular Cell, Kim et al. (2012) identify WTX modulation of p53 tumor-suppressor activity through regulation of p53 acetylation. Therefore, WTX differentially regulates the oncogenic β-catenin pathway and the tumor-suppressing p53 pathway. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2012.02.010
更新日期:2012-03-09 00:00:00
abstract::In this issue, Roberts et al. (2014) describe how hexokinase and mTORC1 cooperate to sense disequilibrium between glucose uptake and utilization and direct the balance of anabolism and catabolism to ensure the appropriate use of cellular resources. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2014.02.009
更新日期:2014-02-20 00:00:00
abstract::Linkage-specific polyubiquitin recognition is thought to make possible the diverse set of functional outcomes associated with ubiquitination. Thus far, mechanistic insight into this selectivity has been largely limited to single domains that preferentially bind to lysine 48-linked polyubiquitin (K48-polyUb) in isolati...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2009.02.011
更新日期:2009-03-27 00:00:00
abstract::High plasticity to utilize different nutrients to adapt metabolic stress is one of the hallmarks for cancer cells. However, the underlying mechanisms by which cancer cells reprogram metabolic machinery in response to metabolic stress remain largely unclear. In this issue of Molecular Cell, Wang et al. (2019) report th...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2019.09.002
更新日期:2019-10-03 00:00:00
abstract::Nutrients are not only organic compounds fueling bioenergetics and biosynthesis, but also key chemical signals controlling growth and metabolism. Nutrients enormously impact the production of reactive oxygen species (ROS), which play essential roles in normal physiology and diseases. How nutrient signaling is integrat...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.03.029
更新日期:2018-05-03 00:00:00
abstract::The hairpin ribozyme is a self-cleaving motif found in the negatives strand of the satellite RNA of some plant viruses. In its natural context, the ribozyme comprises four helices, two of which contain conserved formally unpaired loops, that are adjacent arms of a four-way RNA junction. We show that the arms that woul...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80086-6
更新日期:1998-05-01 00:00:00
abstract::The interaction between pRB and E2F is critical for control of the cell cycle and apoptosis. Here we report that pRB contains two distinct E2F binding sites. The previously identified E2F binding site on pRB is necessary for stable association with E2Fs on DNA. A second E2F interaction site is located entirely within ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00344-7
更新日期:2003-09-01 00:00:00
abstract::Posttranslational histone modifications play important roles in transcription and other chromatin-based processes. Compared to acetylation, methylation, and phosphorylation, very little is known about the function of histone ubiquitylation. Here, we report the purification and functional characterization of a histone ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.03.035
更新日期:2006-05-05 00:00:00
abstract::Tumor cells undergo changes in metabolism to meet their energetic and anabolic needs. It is conceivable that mechanisms exist to sense these changes and link them to pathways that eradicate cells primed for cancer development. We report that the tumor suppressor p53 activates a cell death priming mechanism that senses...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2013.03.016
更新日期:2013-05-09 00:00:00
abstract::Eukaryotic transcription and mRNA processing depend upon the coordinated interactions of many proteins, including Spn1 and Spt6, which are conserved across eukaryotes, are essential for viability, and associate with each other in some of their biologically important contexts. Here we report crystal structures of the S...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2010.11.014
更新日期:2010-12-10 00:00:00
abstract::Signaling by TGFbeta regulates the expression of hundreds of genes. The rapid repression of c-Myc stands out because of its roles in growth control and cancer. Recent work shows that c-Myc repression by TGFbeta is mediated by the nuclear translocation of a novel, preformed complex composed of Smad3, E2F4 or E2F5, and ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00584-1
更新日期:2002-07-01 00:00:00