Essential role for the mammalian ATG8 isoform LC3C in xenophagy.

abstract：:Multi-subunit SMC ATPases control chromosome superstructure and DNA topology, presumably by DNA translocation and loop extrusion. Chromosomal DNA is entrapped within the tripartite SMCkleisin ring. Juxtaposed SMC heads ("J heads") or engaged SMC heads ("E heads") split the SMCkleisin ring into "S" and "K" sub-compartm...

journal_title：Molecular cell

authors： Vazquez Nunez R,Ruiz Avila LB,Gruber S

更新日期：2019-07-25 00:00:00

abstract：:Organisms must survive a variety of stressful conditions, including sudden temperature increases that damage important cellular structures and interfere with essential functions. In response to heat stress, cells activate an ancient signaling pathway leading to the transient expression of heat shock or heat stress pro...

journal_title：Molecular cell

authors： Richter K,Haslbeck M,Buchner J

更新日期：2010-10-22 00:00:00

abstract：:Baculoviral IAP repeat proteins (BIRPs) may affect cell death, cell division, and tumorigenesis. The C. elegans BIRP BIR-1 was localized to chromosomes and to the spindle midzone. Embryos and fertilized oocytes lacking BIR-1 had defects in chromosome behavior, spindle midzone formation, and cytokinesis. We observed in...

journal_title：Molecular cell

authors： Speliotes EK,Uren A,Vaux D,Horvitz HR

更新日期：2000-08-01 00:00:00

abstract：:In yeast Saccharomyces cerevisiae the G protein betagamma subunits (Ste4/Ste18) have long been known to transmit the signal required for mating. Here we demonstrate that GTPase-deficient mutants of Galpha (Gpa1) directly activate the mating response pathway. We also show that signaling by activated Gpa1 requires direc...

journal_title：Molecular cell

authors： Guo M,Aston C,Burchett SA,Dyke C,Fields S,Rajarao SJ,Uetz P,Wang Y,Young K,Dohlman HG

更新日期：2003-08-01 00:00:00

abstract：:Heterotrimeric G proteins communicate signals from activated G protein-coupled receptors to downstream effector proteins. In the phototransduction pathway responsible for vertebrate vision, the G protein-effector complex is composed of the GTP-bound transducin α subunit (GαT·GTP) and the cyclic GMP (cGMP) phosphodiest...

journal_title：Molecular cell

authors： Gao Y,Eskici G,Ramachandran S,Poitevin F,Seven AB,Panova O,Skiniotis G,Cerione RA

更新日期：2020-10-15 00:00:00

abstract：:Forkhead box O (FOXO) transcription factors, the key regulators of cell survival, are negatively controlled through the PI3K-Akt signaling pathway. Phosphorylation of FOXO by Akt leads to cytoplasmic localization and subsequent degradation via the ubiquitin-proteasome system. Here we show a paradigm of FOXO1 regulatio...

journal_title：Molecular cell

authors： Yamagata K,Daitoku H,Takahashi Y,Namiki K,Hisatake K,Kako K,Mukai H,Kasuya Y,Fukamizu A

更新日期：2008-10-24 00:00:00

abstract：:In this issue of Molecular Cell,Sharma et al. (2019) show that normal cell growth requires conversion of an arginine residue in the RNA polymerase II C-terminal domain (CTD) to citrulline, uncovering a potential regulatory pathway involving opposing arginine modifications. ...

journal_title：Molecular cell

authors： Corden JL

更新日期：2019-01-03 00:00:00

abstract：:Type II topoisomerases are essential for resolving topologically entwined double-stranded DNA. Although anti-topoisomerase 2 (Top2) drugs are clinically important antibiotics and chemotherapies, to our knowledge, the mechanisms of cell killing by Top2 depletion and inactivation have never been directly compared. We sh...

journal_title：Molecular cell

authors： Baxter J,Diffley JF

更新日期：2008-06-20 00:00:00

abstract：:Nuclear accumulation of β-catenin, a widely recognized marker of poor cancer prognosis, drives cancer cell proliferation and senescence bypass and regulates incretins, critical regulators of fat and glucose metabolism. Diabetes, characterized by elevated blood glucose levels, is associated with increased cancer risk, ...

journal_title：Molecular cell

authors： Chocarro-Calvo A,García-Martínez JM,Ardila-González S,De la Vieja A,García-Jiménez C

更新日期：2013-02-07 00:00:00

abstract：:Glaucoma, a blinding neurodegenerative disease, whose risk factors include elevated intraocular pressure (IOP), age, and genetics, is characterized by accelerated and progressive retinal ganglion cell (RGC) death. Despite decades of research, the mechanism of RGC death in glaucoma is still unknown. Here, we demonstrat...

journal_title：Molecular cell

authors： Skowronska-Krawczyk D,Zhao L,Zhu J,Weinreb RN,Cao G,Luo J,Flagg K,Patel S,Wen C,Krupa M,Luo H,Ouyang H,Lin D,Wang W,Li G,Xu Y,Li O,Chung C,Yeh E,Jafari M,Ai M,Zhong Z,Shi W,Zheng L,Krawczyk M,Chen D,Sh

更新日期：2015-09-17 00:00:00

abstract：:Innate immune responses are critical for the immediate protection against microbial infection. In Drosophila, infection leads to the rapid and robust production of antimicrobial peptides through two NF-kappaB signaling pathways-IMD and Toll. The IMD pathway is triggered by DAP-type peptidoglycan, common to most Gram-n...

journal_title：Molecular cell

authors： Paquette N,Broemer M,Aggarwal K,Chen L,Husson M,Ertürk-Hasdemir D,Reichhart JM,Meier P,Silverman N

更新日期：2010-01-29 00:00:00

abstract：:Yeast histone H2A is phosphorylated on Ser129 upon DNA damage, an event required for efficient repair. We show that phosphorylation occurs rapidly over a large region around DNA double-strand breaks (DSBs). Histone H4 acetylation is also important for DSB repair, and we found that the NuA4 HAT complex associates speci...

journal_title：Molecular cell

authors： Downs JA,Allard S,Jobin-Robitaille O,Javaheri A,Auger A,Bouchard N,Kron SJ,Jackson SP,Côté J

更新日期：2004-12-22 00:00:00

abstract：:Bcr-Abl is a dysregulated tyrosine kinase whose mechanism of activation is unclear. Here, we demonstrate that, like c-Abl, Bcr-Abl is negatively regulated through its SH3 domain. Kinase activity, transformation, and leukemogenesis by Bcr-Abl are greatly impaired by mutations of the Bcr coiled-coil domain that disrupt ...

journal_title：Molecular cell

authors： Smith KM,Yacobi R,Van Etten RA

更新日期：2003-07-01 00:00:00

abstract：:Both H4K16 acetylation and H3K4 trimethylation are required for gene activation. However, it is still largely unclear how these modifications are orchestrated by transcriptional factors. Here, we analyzed the mechanism of the transcriptional activation by FOXP3, an X-linked suppressor of autoimmune diseases and cancer...

journal_title：Molecular cell

authors： Katoh H,Qin ZS,Liu R,Wang L,Li W,Li X,Wu L,Du Z,Lyons R,Liu CG,Liu X,Dou Y,Zheng P,Liu Y

更新日期：2011-12-09 00:00:00

abstract：:Upon heterologous overexpression, many proteins misfold or aggregate, thus resulting in low functional yields. Human acetylcholinesterase (hAChE), an enzyme mediating synaptic transmission, is a typical case of a human protein that necessitates mammalian systems to obtain functional expression. We developed a computat...

journal_title：Molecular cell

authors： Goldenzweig A,Goldsmith M,Hill SE,Gertman O,Laurino P,Ashani Y,Dym O,Unger T,Albeck S,Prilusky J,Lieberman RL,Aharoni A,Silman I,Sussman JL,Tawfik DS,Fleishman SJ

更新日期：2016-07-21 00:00:00

abstract：:Downstream core promoter elements are an expanding class of regulatory sequences that add considerable diversity to the promoter architecture of RNA polymerase II-transcribed genes. We set out to determine the factors necessary for downstream promoter element (DPE)-dependent transcription and find that, against expect...

journal_title：Molecular cell

authors： Lewis BA,Sims RJ 3rd,Lane WS,Reinberg D

更新日期：2005-05-13 00:00:00

abstract：:Stress granules (SGs) are condensates of mRNPs that form in response to stress. SGs arise by multivalent protein-protein, protein-RNA, and RNA-RNA interactions. However, the role of RNA-RNA interactions in SG assembly remains understudied. Here, we describe a yeast SG reconstitution system that faithfully recapitulate...

journal_title：Molecular cell

authors： Begovich K,Wilhelm JE

更新日期：2020-09-17 00:00:00

abstract：:Telomeres protect chromosome ends from fusing to double-stranded breaks (DSBs). Using a quantitative real-time PCR assay, we show that nonhomologous end joining between a telomere and an inducible DSB was undetectable in wild-type cells, but occurred within a few hours of DSB induction in approximately 1/2000 genomes ...

journal_title：Molecular cell

authors： Chan SW,Blackburn EH

更新日期：2003-05-01 00:00:00

abstract：:S(N)1-type alkylating agents that produce cytotoxic O(6)-methyl-G (O(6)-meG) DNA adducts induce cell cycle arrest and apoptosis in a manner requiring the DNA mismatch repair (MMR) proteins MutSalpha and MutLalpha. Here, we show that checkpoint signaling in response to DNA methylation occurs during S phase and requires...

journal_title：Molecular cell

authors： Yoshioka K,Yoshioka Y,Hsieh P

更新日期：2006-05-19 00:00:00

abstract：:The essential functions of the conserved Smc5/6 complex remain elusive. To uncover its roles in genome maintenance, we established Saccharomyces cerevisiae cell-cycle-regulated alleles that enable restriction of Smc5/6 components to S or G2/M. Unexpectedly, the essential functions of Smc5/6 segregated fully and select...

journal_title：Molecular cell

authors： Menolfi D,Delamarre A,Lengronne A,Pasero P,Branzei D

更新日期：2015-12-17 00:00:00

abstract：:Budding yeast Cdc13, Stn1, and Ten1 form the CST complex to protect telomeres from lethal DNA degradation. It remains unknown whether similar complexes are conserved in higher eukaryotes or not. Here we isolated mammalian STN1 and TEN1 homologs and CTC1 (conserved telomere maintenance component 1). The three proteins ...

journal_title：Molecular cell

authors： Miyake Y,Nakamura M,Nabetani A,Shimamura S,Tamura M,Yonehara S,Saito M,Ishikawa F

更新日期：2009-10-23 00:00:00

abstract：:Cells constantly adjust their metabolism in response to environmental conditions, yet major mechanisms underlying survival remain poorly understood. We discover a posttranscriptional mechanism that integrates starvation response with GTP homeostasis to allow survival, enacted by the nucleotide (p)ppGpp, a key player i...

journal_title：Molecular cell

authors： Kriel A,Bittner AN,Kim SH,Liu K,Tehranchi AK,Zou WY,Rendon S,Chen R,Tu BP,Wang JD

更新日期：2012-10-26 00:00:00

abstract：:To determine whether replicational mutagenesis in the yeast genome is influenced by the positions of active origins, a reporter gene was placed in two orientations at multiple locations within a 39,000 bp region of chromosome III possessing two strong origins. The frequency of mutations resulting from misincorporation...

journal_title：Molecular cell

authors： Pavlov YI,Newlon CS,Kunkel TA

更新日期：2002-07-01 00:00:00

abstract：:Mammalian target of rapamycin (mTOR) activity is regulated by assembly of two functionally distinct complexes, mTORC1 and mTORC2. In syndecan-4 (S4) null endothelial cells, mTORC2 activity is reduced, resulting in decreased Akt activation, while mTORC1 activity is increased. Levels of rictor, mLST8, and mSin-1 are unc...

journal_title：Molecular cell

authors： Partovian C,Ju R,Zhuang ZW,Martin KA,Simons M

更新日期：2008-10-10 00:00:00

abstract：:The glycogen synthase kinase-3 (GSK3) family kinases are central cellular regulators highly conserved in all eukaryotes. In Arabidopsis, the GSK3-like kinase BIN2 phosphorylates a range of proteins to control broad developmental processes, and BIN2 is degraded through unknown mechanism upon receptor kinase-mediated br...

journal_title：Molecular cell

authors： Zhu JY,Li Y,Cao DM,Yang H,Oh E,Bi Y,Zhu S,Wang ZY

更新日期：2017-06-01 00:00:00

abstract：:The TATA binding protein (TBP) is required for the expression of nearly all genes and is highly regulated both positively and negatively. Here, we use DNA microarrays to explore the genome-wide interplay of several TBP-interacting inhibitors in the yeast Saccharomyces cerevisiae. Our findings suggest the following: Th...

journal_title：Molecular cell

authors： Chitikila C,Huisinga KL,Irvin JD,Basehoar AD,Pugh BF

更新日期：2002-10-01 00:00:00

abstract：:Programmed cell death is a gene-directed process involved in the development and homeostasis of multicellular organisms. The most common mode of programmed cell death is apoptosis, which is characterized by a stereotypical set of biochemical and morphological hallmarks. Here we report that Escherichia coli also exhibi...

journal_title：Molecular cell

authors： Dwyer DJ,Camacho DM,Kohanski MA,Callura JM,Collins JJ

更新日期：2012-06-08 00:00:00

abstract：:Autophagic turnover of intracellular constituents is critical for cellular housekeeping, nutrient recycling, and various aspects of growth and development in eukaryotes. Here we show that autophagy impacts the other major degradative route involving the ubiquitin-proteasome system by eliminating 26S proteasomes, a pro...

journal_title：Molecular cell

authors： Marshall RS,Li F,Gemperline DC,Book AJ,Vierstra RD

更新日期：2015-06-18 00:00:00

abstract：:Study of mutant phenotypes is a fundamental method for understanding gene function. The construction of a near-complete collection of yeast knockouts (YKO) and the unique molecular barcodes (or TAGs) that identify each strain has enabled quantitative functional profiling of Saccharomyces cerevisiae. By using these TAG...

journal_title：Molecular cell

authors： Pan X,Yuan DS,Xiang D,Wang X,Sookhai-Mahadeo S,Bader JS,Hieter P,Spencer F,Boeke JD

更新日期：2004-11-05 00:00:00

abstract：:Human sister chromatids at metaphase are primarily linked by centromeric cohesion, forming the iconic X shape. Premature loss of centromeric cohesion disrupts orderly mitotic progression. Shugoshin (Sgo1) binds to and protects cohesin at inner centromeres. The kinetochore kinase Bub1 phosphorylates histone H2A at T120...

journal_title：Molecular cell

authors： Liu H,Qu Q,Warrington R,Rice A,Cheng N,Yu H

更新日期：2015-08-06 00:00:00