Abstract：
:Downstream core promoter elements are an expanding class of regulatory sequences that add considerable diversity to the promoter architecture of RNA polymerase II-transcribed genes. We set out to determine the factors necessary for downstream promoter element (DPE)-dependent transcription and find that, against expectations, TFIID and the GTFs are not sufficient. Instead, the protein kinase CK2 and the coactivator PC4 establish DPE-specific transcription in an in vitro transcription system containing TFIID, Mediator, and the GTFs. Chromatin immunoprecipitation analyses using the DPE-dependent IRF-1 and TAF7 promoters demonstrated that CK2, and PC4 are present on these promoters in vivo. In contrast, neither PC4 nor CK2 were detected on the TAF1-dependent cyclin D promoter, which contains a DCE type of downstream element. Our findings also demonstrate that CK2 activity alters TFIID-dependent recognition of DCE sequences. These data establish that CK2 acts as a switch, converting the transcriptional machinery from functioning on one type of downstream element to another.
journal_name
Mol Celljournal_title
Molecular cellauthors
Lewis BA,Sims RJ 3rd,Lane WS,Reinberg Ddoi
10.1016/j.molcel.2005.04.005subject
Has Abstractpub_date
2005-05-13 00:00:00pages
471-81issue
4eissn
1097-2765issn
1097-4164pii
S1097-2765(05)01249-9journal_volume
18pub_type
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