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Diverse pathways generate microRNA-like RNAs and Dicer-independent small interfering RNAs in fungi.

Abstract：

:A variety of small RNAs, including the Dicer-dependent miRNAs and the Dicer-independent Piwi-interacting RNAs, associate with Argonaute family proteins to regulate gene expression in diverse cellular processes. These two species of small RNA have not been found in fungi. Here, by analyzing small RNAs associated with the Neurospora Argonaute protein QDE-2, we show that diverse pathways generate miRNA-like small RNAs (milRNAs) and Dicer-independent small interfering RNAs (disiRNAs) in this filamentous fungus. Surprisingly, milRNAs are produced by at least four different mechanisms that use a distinct combination of factors, including Dicers, QDE-2, the exonuclease QIP, and an RNase III domain-containing protein, MRPL3. In contrast, disiRNAs originate from loci producing overlapping sense and antisense transcripts, and do not require the known RNAi components for their production. Taken together, these results uncover several pathways for small RNA production in filamentous fungi, shedding light on the diversity and evolutionary origins of eukaryotic small RNAs.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Lee HC,Li L,Gu W,Xue Z,Crosthwaite SK,Pertsemlidis A,Lewis ZA,Freitag M,Selker EU,Mello CC,Liu Y

doi

10.1016/j.molcel.2010.04.005

subject

Has Abstract

pub_date

2010-06-25 00:00:00

pages

803-14

issue

6

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(10)00282-0

journal_volume

38

pub_type

杂志文章

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