Abstract：
:Transcription and splicing are functionally coupled, resulting in highly efficient splicing of RNA polymerase II (RNAP II) transcripts. The mechanism involved in this coupling is not known. To identify potential coupling factors, we carried out a comprehensive proteomic analysis of immunopurified human RNAP II, identifying >100 specifically associated proteins. Among these are the SR protein family of splicing factors and all of the components of U1 snRNP, but no other snRNPs or splicing factors. We show that SR proteins function in coupling transcription to splicing and provide evidence that the mechanism involves cotranscriptional recruitment of SR proteins to RNAP II transcripts. We propose that the exclusive association of U1 snRNP/SR proteins with RNAP II positions these splicing factors, which are known to function early in spliceosome assembly, close to the nascent pre-mRNA. Thus, these factors readily out-compete inhibitory hnRNP proteins, resulting in efficient spliceosome assembly on nascent RNAP II transcripts.
journal_name
Mol Celljournal_title
Molecular cellauthors
Das R,Yu J,Zhang Z,Gygi MP,Krainer AR,Gygi SP,Reed Rdoi
10.1016/j.molcel.2007.05.036subject
Has Abstractpub_date
2007-06-22 00:00:00pages
867-81issue
6eissn
1097-2765issn
1097-4164pii
S1097-2765(07)00360-7journal_volume
26pub_type
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