Abstract:
:Proteins are translocated across membranes through a channel that is formed by the prokaryotic SecY or eukaryotic Sec61 complex. The crystal structure of the SecY channel from M. jannaschii revealed a plug domain that appears to seal the channel in its closed state. However, the role of the plug remains unclear, particularly because plug deletion mutants in S. cerevisiae are functional. Here, we demonstrate that plug deletion mutants in E. coli SecY are also functional and even efficiently translocate proteins with defective or missing signal sequences. The crystal structures of equivalent plug deletions in SecY of M. jannaschii show that, although the overall structures are maintained, new plugs are formed. These lack many interactions that normally stabilize the closed channel, explaining why the channels can open for proteins with signal-sequence mutations. Our data show that the plug domain is required to maintain a closed state of the channel and suggest a mechanism for channel gating.
journal_name
Mol Celljournal_title
Molecular cellauthors
Li W,Schulman S,Boyd D,Erlandson K,Beckwith J,Rapoport TAdoi
10.1016/j.molcel.2007.05.002subject
Has Abstractpub_date
2007-05-25 00:00:00pages
511-21issue
4eissn
1097-2765issn
1097-4164pii
S1097-2765(07)00260-2journal_volume
26pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract::RNA-binding proteins coordinate the fates of multiple RNAs, but the principles underlying these global interactions remain poorly understood. We elucidated regulatory mechanisms of the RNA-binding protein HuR, by integrating data from diverse high-throughput targeting technologies, specifically PAR-CLIP, RIP-chip, and...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.06.007
更新日期:2011-08-05 00:00:00
abstract::Faithful transmission of genomic information requires tight spatiotemporal regulation of DNA replication factors. In the licensing step of DNA replication, CDT-1 is loaded onto chromatin to subsequently promote the recruitment of additional replication factors, including CDC-45 and GINS. During the elongation step, th...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.08.028
更新日期:2011-10-07 00:00:00
abstract::The anaphase-promoting complex or cyclosome (APC/C) is a ubiquitin ligase that polyubiquitinates specific substrates at precise times in the cell cycle, thereby triggering the events of late mitosis in a strict order. The robust substrate specificity of the APC/C prevents the potentially deleterious degradation of non...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2016.09.006
更新日期:2016-10-06 00:00:00
abstract::Homologous recombination is essential for preserving genome integrity. Joining of homologous DNA molecules through strand exchange, a pivotal step in recombination, is mediated by RAD51. Here, we identify RAD51AP1 as a RAD51 accessory protein that specifically stimulates joint molecule formation through the combinatio...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.08.025
更新日期:2007-11-09 00:00:00
abstract::Copy-number changes generate phenotypic variability in health and disease. Whether organisms protect against copy-number changes is largely unknown. Here, we show that Saccharomyces cerevisiae monitors the copy number of its ribosomal DNA (rDNA) and rapidly responds to copy-number loss with the clonal amplification of...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.08.036
更新日期:2018-11-01 00:00:00
abstract::The phosphoinositide (PI)-3-kinase-related kinase (PIKK) family proteins Tel1p and Mec1p have been implicated in the telomere integrity of Saccharomyces cerevisiae. However, the mechanism of PIKK-mediated telomere length control remains unclear. Here, we show that Tel1p and Mec1p are recruited to the telomeres at spec...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00262-x
更新日期:2004-05-21 00:00:00
abstract::A chemicogenetic screen was performed in budding yeast mutants that have a weakened replication stress response. This identified an inhibitor of target of rapamycin (TOR) complexes 1 and 2 that selectively enhances the sensitivity of sgs1Δ cells to hydroxyurea and camptothecin. More importantly, the inhibitor has stro...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2013.08.019
更新日期:2013-09-26 00:00:00
abstract::Kong et al. (2020) present the low-resolution structure of the ATPɣS-bound human condensin I and II complexes and demonstrate that human condensins can extrude DNA loops in a symmetric and asymmetric fashion and compact nucleosome-bound DNA. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2020.06.025
更新日期:2020-07-02 00:00:00
abstract::The Ca2+ -activated K+ channel KCa3.1 is required for Ca2+ influx and the subsequent activation of B and T cells. Inhibitors of KCa3.1 are in development to treat autoimmune diseases and transplant rejection, underscoring the importance in understanding how these channels are regulated. We show that nucleoside diphosp...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.11.012
更新日期:2006-12-08 00:00:00
abstract::In this issue of Molecular Cell, Chereji et al. (2017) present new data on MNase-sensitive particles previously identified upstream of transcription start sites at many promoters in budding yeast, and they argue, based upon negative histone-ChIP results, that they are non-nucleosomal signals generated by transcription...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.01.010
更新日期:2017-02-02 00:00:00
abstract::Argonaute proteins are the mediators of small RNA-guided gene silencing pathways. In this issue, Couvillion and coworkers (Couvillion et al., 2012) found an unexpected function for a Tetrahymena Argonaute protein: It forms a complex with tRNA fragments and is required for nuclear Xrn2 localization and function. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2012.11.015
更新日期:2012-11-30 00:00:00
abstract::The RNA-binding protein HuR, while known to stabilize cytoplasmic mRNAs, is largely nuclear. In this issue of Molecular Cell, Mukherjee et al. (2011) and Lebedeva et al. (2011) identify transcriptome-wide HuR-RNA interactions using PAR-CLIP, unveiling HuR's nuclear role in pre-mRNA processing. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2011.07.016
更新日期:2011-08-05 00:00:00
abstract::EGFR activates phosphatidylinositide 3-kinase (PI3K), but the mechanism underlying this activation is not completely understood. We demonstrated here that EGFR activation resulted in lysine acetyltransferase 5 (KAT5)-mediated K395 acetylation of the platelet isoform of phosphofructokinase 1 (PFKP) and subsequent trans...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.03.018
更新日期:2018-04-19 00:00:00
abstract::Bacterial actin MreB is one of the key components of the bacterial cytoskeleton. It assembles into short filaments that lie just underneath the membrane and organize the cell wall synthesis machinery. Here we show that MreB from both T. maritima and E. coli binds directly to cell membranes. This function is essential ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.07.008
更新日期:2011-08-05 00:00:00
abstract::Dysregulation of Wnt signaling has been implicated in tumorigenesis. The role of Transducin β-like proteins TBL1-TBLR1 in the promotion of Wnt/β-catenin-mediated oncogenesis has recently been emphasized; however, the molecular basis of activation of Wnt signaling by the corepressor TBL1-TBLR1 is incompletely understoo...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.05.027
更新日期:2011-07-22 00:00:00
abstract::YAP/TEAD are nuclear effectors of the Hippo pathway, regulating organ size and tumorigenesis largely through promoter-associated function. However, their function as enhancer regulators remains poorly understood. Through an in vivo proximity-dependent labeling (BioID) technique, we identified YAP1 and TEAD4 protein as...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2019.06.010
更新日期:2019-08-22 00:00:00
abstract::N6-methyladenosine (m6A) and adenosine-to-inosine (A-to-I) editing are two of the most abundant RNA modifications, both at adenosines. Yet, the interaction of these two types of adenosine modifications is largely unknown. Here we show a global A-to-I difference between m6A-positive and m6A-negative RNA populations. Bo...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.12.006
更新日期:2018-01-04 00:00:00
abstract::Communication between the 5' cap structure and 3' poly(A) tail of eukaryotic mRNA results in the synergistic enhancement of translation. The cap and poly(A) tail binding proteins, eIF4E and Pab1p, mediate this effect in the yeast S. cerevisiae through their interactions with different parts of the translation factor e...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80122-7
更新日期:1998-07-01 00:00:00
abstract::DNA replication forks pause in front of lesions on the template, eventually leading to cytotoxic chromosomal rearrangements. The in vivo structure of damaged eukaryotic replication intermediates has been so far elusive. Combining electron microscopy (EM) and two-dimensional (2D) gel electrophoresis, we found that UV-i...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.11.015
更新日期:2006-01-06 00:00:00
abstract::While gapmers efficiently knock down as well as terminate transcription of nascent lncRNAs and mRNAs, Lee and Mendell (2020) and Lai et al. (2020) also demonstrate that Pol II termination is not observed with gapmers targeting the 3' terminal portions of the transcript. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2020.02.010
更新日期:2020-03-05 00:00:00
abstract::To map the genomic interaction sites of chromatin proteins, two related methods were developed and experimentally explored in Saccharomyces cerevisiae. The ChIC method (chromatin immunocleavage) consists of tethering a fusion protein (pA-MN) consisting of micrococcal nuclease (MN) and staphylococcal protein A to speci...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.09.007
更新日期:2004-10-08 00:00:00
abstract::In this issue of Molecular Cell, Pont et al. (2012) show that AUF1/hnRNP D promotes TERT transcription, which is required for telomere maintenance in mice. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2012.06.031
更新日期:2012-07-13 00:00:00
abstract::The activity of the tumor suppressor p53 has to be timed and balanced closely to prevent untimely induction of cell death. The stability of p53 depends on the ubiquitin ligase Mdm2 but also on Hsp70 and Hsp90 chaperones that interact with its DNA binding domain (DBD). Using hydrogen exchange mass spectrometry and bioc...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2019.03.032
更新日期:2019-05-16 00:00:00
abstract::Cardiac disease remains the leading cause of morbidity and mortality worldwide. The β1-adrenergic receptor (β1-AR) is a major regulator of cardiac functions and is downregulated in the majority of heart failure cases. A key physiological process is the activation of heterotrimeric G-protein Gs by β1-ARs, leading to in...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2020.08.001
更新日期:2020-10-01 00:00:00
abstract::Mammalian lefty and zebrafish antivin form a subgroup of the TGF beta superfamily. We report that mouse mutants for lefty2 have an expanded primitive streak and form excess mesoderm, a phenotype opposite to that of mutants for the TGF beta gene nodal. Analogously, overexpression of Antivin or Lefty2 in zebrafish embry...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80331-7
更新日期:1999-09-01 00:00:00
abstract::In this issue of Molecular Cell,Sharma et al. (2019) show that normal cell growth requires conversion of an arginine residue in the RNA polymerase II C-terminal domain (CTD) to citrulline, uncovering a potential regulatory pathway involving opposing arginine modifications. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2018.12.013
更新日期:2019-01-03 00:00:00
abstract::Angiogenin is a stress-activated ribonuclease that cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs). Transfection of natural or synthetic tiRNAs inhibits protein synthesis and triggers the phospho-eIF2α-independent assembly of stress granules (SGs), essential components of ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.06.022
更新日期:2011-08-19 00:00:00
abstract::The dynamic protein interactions required for transcription are functionally important yet poorly understood; in this issue of Molecular Cell, Zobeck et al. (2010) resolve the sequential recruitment and selective recycling of transcription factors at an actively transcribing locus in Drosophila. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2010.12.010
更新日期:2010-12-22 00:00:00
abstract::Prenylated Rab proteins exist in the cytosol as soluble, high-affinity complexes with GDI that need to be disrupted for membrane attachment and targeting of Rab proteins. The Legionella pneumophila protein DrrA displaces GDI from Rab1:GDI complexes, incorporating Rab1 into Legionella-containing vacuoles and activating...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2009.11.014
更新日期:2009-12-25 00:00:00
abstract::Concomitant with DNA replication, the chromosomal cohesin complex establishes cohesion between newly replicated sister chromatids. Several replication-fork-associated "cohesion establishment factors," including the multifunctional Ctf18-RFC complex, aid this process in as yet unknown ways. Here, we show that Ctf18-RFC...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2020.03.017
更新日期:2020-05-21 00:00:00