Reciprocal association of the budding yeast ATM-related proteins Tel1 and Mec1 with telomeres in vivo.

abstract：:The phosphatidylinositol 3-kinase (PI3K) signaling pathway is frequently deregulated in cancer. Downstream of PI3K, Akt1 and Akt2 have opposing roles in breast cancer invasive migration, leading to metastatic dissemination. Here, we identify palladin, an actin-associated protein, as an Akt1-specific substrate that mod...

journal_title：Molecular cell

authors： Chin YR,Toker A

更新日期：2010-05-14 00:00:00

abstract：:DNA replication forks pause in front of lesions on the template, eventually leading to cytotoxic chromosomal rearrangements. The in vivo structure of damaged eukaryotic replication intermediates has been so far elusive. Combining electron microscopy (EM) and two-dimensional (2D) gel electrophoresis, we found that UV-i...

journal_title：Molecular cell

authors： Lopes M,Foiani M,Sogo JM

更新日期：2006-01-06 00:00:00

abstract：:Nucleosomes are barriers to transcription in vitro; however, their effects on RNA polymerase in vivo are unknown. Here we describe a simple and general strategy to comprehensively map the positions of elongating and arrested RNA polymerase II (RNAPII) at nucleotide resolution. We find that the entry site of the first ...

journal_title：Molecular cell

authors： Weber CM,Ramachandran S,Henikoff S

更新日期：2014-03-06 00:00:00

abstract：:Heterotrimeric G proteins communicate signals from activated G protein-coupled receptors to downstream effector proteins. In the phototransduction pathway responsible for vertebrate vision, the G protein-effector complex is composed of the GTP-bound transducin α subunit (GαT·GTP) and the cyclic GMP (cGMP) phosphodiest...

journal_title：Molecular cell

authors： Gao Y,Eskici G,Ramachandran S,Poitevin F,Seven AB,Panova O,Skiniotis G,Cerione RA

更新日期：2020-10-15 00:00:00

abstract：:BRCA1 and BRCA2 account for most cases of familial, early onset breast and/or ovarian cancer and encode products that each interact with hRAD51. Results presented here show that BRCA1 and BRCA2 coexist in a biochemical complex and colocalize in subnuclear foci in somatic cells and on the axial elements of developing s...

journal_title：Molecular cell

authors： Chen J,Silver DP,Walpita D,Cantor SB,Gazdar AF,Tomlinson G,Couch FJ,Weber BL,Ashley T,Livingston DM,Scully R

更新日期：1998-09-01 00:00:00

abstract：:There are few tools available for dissecting and elucidating the functions of DNA satellites and other nongenic DNA. To address this, we have explored the experimental potential of DNA sequence-specific drugs containing pyrrole and imidazole amino acids (polyamides). Compounds were synthesized that target different Dr...

journal_title：Molecular cell

authors： Janssen S,Durussel T,Laemmli UK

更新日期：2000-11-01 00:00:00

abstract：:Interference with nucleocytoplasmic transport is a strategy employed by certain viruses to compromise host cellular function. While it has been shown that the matrix (M) protein of the vesicular stomatitis virus (VSV) inhibits nuclear export of host cell mRNAs, the underlying mechanism has not been fully established. ...

journal_title：Molecular cell

authors： Faria PA,Chakraborty P,Levay A,Barber GN,Ezelle HJ,Enninga J,Arana C,van Deursen J,Fontoura BM

更新日期：2005-01-07 00:00:00

abstract：:The separation of sister chromatids in anaphase depends on the dissociation of cohesin from chromosomes. In vertebrates, some cohesin is removed from chromosomes at the onset of anaphase by proteolytic cleavage. In contrast, the bulk of cohesin is removed from chromosomes already in prophase and prometaphase by an unk...

journal_title：Molecular cell

authors： Sumara I,Vorlaufer E,Stukenberg PT,Kelm O,Redemann N,Nigg EA,Peters JM

更新日期：2002-03-01 00:00:00

abstract：:Group II intron RNPs are mobile genetic elements that attack and invade duplex DNA. In this work, we monitor the invasion reaction in vitro and establish a quantitative kinetic framework for the steps of this complex cascade. We find that target site specificity is achieved after DNA binding, which occurs nonspecifica...

journal_title：Molecular cell

authors： Aizawa Y,Xiang Q,Lambowitz AM,Pyle AM

更新日期：2003-03-01 00:00:00

abstract：:Errors in translation cause cytotoxic protein misfolding and aggregation. In this issue, Ling et al. (2012) show that scavenging or suppression of reactive oxygen species in E. coli reduces the cellular costs of error-induced aggregation. ...

journal_title：Molecular cell

authors： Drummond DA

更新日期：2012-12-14 00:00:00

abstract：:Multisubunit protein complexes pose a challenge to the coordinated regulation of individual components. We show how the yeast transactivating factor Met4 functions as a component of the SCF(Met30) ubiquitin ligase to synchronize its own activity with cofactor assembly. Cells maintain Met4 in a dormant state by a regul...

journal_title：Molecular cell

authors： Ouni I,Flick K,Kaiser P

更新日期：2010-12-22 00:00:00

abstract：:The mechanisms by which telomeres are distinguished from DNA double-strand breaks are poorly understood. Here we have defined the minimal requirements for the protection of telomeric DNA ends from nonhomologous end-joining (NHEJ). Neither long, single-stranded overhangs nor t loop formation is essential to prevent NHE...

journal_title：Molecular cell

authors： Bae NS,Baumann P

更新日期：2007-05-11 00:00:00

abstract：:RING ubiquitin ligases (E3) recruit ubiquitin-conjugate enzymes (E2) charged with ubiquitin (Ub) to catalyze ubiquitination. Non-covalent Ub binding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains elusive. Here, we show that backside bound Ub (Ub(B)) enhances both RING-in...

journal_title：Molecular cell

authors： Buetow L,Gabrielsen M,Anthony NG,Dou H,Patel A,Aitkenhead H,Sibbet GJ,Smith BO,Huang DT

更新日期：2015-04-16 00:00:00

abstract：:The double Holliday junction (dHJ) is generally regarded to be a key intermediate of meiotic recombination, whose resolution is critical for the formation of crossover recombinants. In fission yeast, the Mus81-Eme1 endonuclease has been implicated in resolving dHJs. Consistent with this role, we show that Mus81-Eme1 i...

journal_title：Molecular cell

authors： Osman F,Dixon J,Doe CL,Whitby MC

更新日期：2003-09-01 00:00:00

abstract：:The MHC class I antigen presentation system enables T cell immunosurveillance of cancers and viruses. A substantial fraction of the immunopeptidome derives from rapidly degraded nascent polypeptides (DRiPs). By knocking down each of the 80 ribosomal proteins, we identified proteins that modulate peptide generation wit...

journal_title：Molecular cell

authors： Wei J,Kishton RJ,Angel M,Conn CS,Dalla-Venezia N,Marcel V,Vincent A,Catez F,Ferré S,Ayadi L,Marchand V,Dersh D,Gibbs JS,Ivanov IP,Fridlyand N,Couté Y,Diaz JJ,Qian SB,Staudt LM,Restifo NP,Yewdell JW

更新日期：2019-03-21 00:00:00

abstract：:Telomeres protect chromosome ends from fusing to double-stranded breaks (DSBs). Using a quantitative real-time PCR assay, we show that nonhomologous end joining between a telomere and an inducible DSB was undetectable in wild-type cells, but occurred within a few hours of DSB induction in approximately 1/2000 genomes ...

journal_title：Molecular cell

authors： Chan SW,Blackburn EH

更新日期：2003-05-01 00:00:00

abstract：:In response to hyperosmotic environments, most eukaryotic cells activate a specialized mitogen-activated protein (MAP) kinase pathway. In S. cerevisiae, the key protein kinase, Hog1, coordinates the transcriptional induction of a variety of genes devoted to osmoadaptation and general stress protection. Depending on th...

journal_title：Molecular cell

authors： Alepuz PM,Jovanovic A,Reiser V,Ammerer G

更新日期：2001-04-01 00:00:00

abstract：:Chromatin governs gene regulation and genome maintenance, yet a substantial fraction of the chromatin proteome is still unexplored. Moreover, a global model of the chromatin protein network is lacking. By screening >100 candidates we identify 42 Drosophila proteins that were not previously associated with chromatin, w...

journal_title：Molecular cell

authors： van Bemmel JG,Filion GJ,Rosado A,Talhout W,de Haas M,van Welsem T,van Leeuwen F,van Steensel B

更新日期：2013-02-21 00:00:00

abstract：:The Raf/MEK/ERK and PI3K/Akt pathways are prominent effectors of oncogenic Ras. These pathways negatively regulate each other, but the mechanism involved is incompletely understood. We now identify MEK1 as an essential regulator of lipid/protein phosphatase PTEN, through which it controls phosphatidylinositol-3-phosph...

journal_title：Molecular cell

authors： Zmajkovicova K,Jesenberger V,Catalanotti F,Baumgartner C,Reyes G,Baccarini M

更新日期：2013-04-11 00:00:00

abstract：:We generated knockout mice for MCM8 and MCM9 and show that deficiency for these genes impairs homologous recombination (HR)-mediated DNA repair during gametogenesis and somatic cells cycles. MCM8(-/-) mice are sterile because spermatocytes are blocked in meiotic prophase I, and females have only arrested primary folli...

journal_title：Molecular cell

authors： Lutzmann M,Grey C,Traver S,Ganier O,Maya-Mendoza A,Ranisavljevic N,Bernex F,Nishiyama A,Montel N,Gavois E,Forichon L,de Massy B,Méchali M

更新日期：2012-08-24 00:00:00

abstract：:Streptolydigin (Stl) is a potent inhibitor of bacterial RNA polymerases (RNAPs). The 2.4 A resolution structure of the Thermus thermophilus RNAP-Stl complex showed that, in full agreement with the available genetic data, the inhibitor binding site is located 20 A away from the RNAP active site and encompasses the brid...

journal_title：Molecular cell

authors： Temiakov D,Zenkin N,Vassylyeva MN,Perederina A,Tahirov TH,Kashkina E,Savkina M,Zorov S,Nikiforov V,Igarashi N,Matsugaki N,Wakatsuki S,Severinov K,Vassylyev DG

更新日期：2005-09-02 00:00:00

abstract：:Ubiquitin-dependent proteolysis is an important mechanism that suppresses the beta-catenin transcription factor in cells without Wnt stimulation. A critical step in this regulatory pathway is to create a SCF(beta-TrCP) E3 ubiquitin ligase binding site for beta-catenin. Here we show that the SCF(beta-TrCP) binding site...

journal_title：Molecular cell

authors： Su Y,Fu C,Ishikawa S,Stella A,Kojima M,Shitoh K,Schreiber EM,Day BW,Liu B

更新日期：2008-12-05 00:00:00

abstract：:HAT1 is an essential gene in mammals that acetylates newly synthesized histone H4 and is involved in chromatin assembly and genome maintenance. In this issue of Molecular Cell, Gruber et al. (2019) show that HAT1 binds to and stimulates H4 gene expression in a growth-factor- and glucose-dependent manner. ...

journal_title：Molecular cell

authors： Denu JM

更新日期：2019-08-22 00:00:00

abstract：:Bacterial actin MreB is one of the key components of the bacterial cytoskeleton. It assembles into short filaments that lie just underneath the membrane and organize the cell wall synthesis machinery. Here we show that MreB from both T. maritima and E. coli binds directly to cell membranes. This function is essential ...

journal_title：Molecular cell

authors： Salje J,van den Ent F,de Boer P,Löwe J

更新日期：2011-08-05 00:00:00

abstract：:The protective "cap" that assembles at chromosome ends recruits and controls an intricate network of biochemical activities, each one critical for telomere structure and the maintenance of genomic stability. Recent studies have uncovered the components of telomere caps and have started to define the pathways that lead...

journal_title：Molecular cell

authors： Ferreira MG,Miller KM,Cooper JP

更新日期：2004-01-16 00:00:00

abstract：:When faced with proteotoxic stress, cells mount adaptive responses to eliminate aberrant proteins. Adaptive responses increase the expression of protein folding and degradation factors to enhance the cellular quality control machinery. However, it is unclear whether and how this augmented machinery acquires new activi...

journal_title：Molecular cell

authors： Szoradi T,Schaeff K,Garcia-Rivera EM,Itzhak DN,Schmidt RM,Bircham PW,Leiss K,Diaz-Miyar J,Chen VK,Muzzey D,Borner GHH,Schuck S

更新日期：2018-06-21 00:00:00

abstract：:Faithful duplication of the genome in S phase followed by its accurate segregation in mitosis is essential to maintain genomic integrity. Recent studies have suggested that proteins involved in DNA transactions are also required for whole-chromosome stability. Here we demonstrate that the MRN (Mre11, Rad50, and Nbs1) ...

journal_title：Molecular cell

authors： Rozier L,Guo Y,Peterson S,Sato M,Baer R,Gautier J,Mao Y

更新日期：2013-03-28 00:00:00

abstract：:Recycling of RNA polymerase II (Pol II) after transcription requires dephosphorylation of the polymerase C-terminal domain (CTD) by the phosphatase Fcp1. We report the X-ray structure of the small CTD phosphatase Scp1, which is homologous to the Fcp1 catalytic domain. The structure shows a core fold and an active cent...

journal_title：Molecular cell

authors： Kamenski T,Heilmeier S,Meinhart A,Cramer P

更新日期：2004-08-13 00:00:00

abstract：:Mutations within BRCA1 predispose carriers to a high risk of breast and ovarian cancers. BRCA1 functions to maintain genomic stability through the assembly of multiple protein complexes involved in DNA repair, cell-cycle arrest, and transcriptional regulation. Here, we report the identification of a DNA damage-induced...

journal_title：Molecular cell

authors： Savage KI,Gorski JJ,Barros EM,Irwin GW,Manti L,Powell AJ,Pellagatti A,Lukashchuk N,McCance DJ,McCluggage WG,Schettino G,Salto-Tellez M,Boultwood J,Richard DJ,McDade SS,Harkin DP

更新日期：2014-05-08 00:00:00

abstract：:c-Jun, a major transcription factor in the activating protein 1 (AP-1) family of regulatory proteins, is activated by many physiologic and pathologic stimuli. However, whether c-jun is regulated by epigenetic modification of chromatin structure is not clear. We showed here that c-jun was transcriptionally repressed in...

journal_title：Molecular cell

authors： Xia Y,Wang J,Liu TJ,Yung WK,Hunter T,Lu Z

更新日期：2007-01-26 00:00:00