Abstract:
:The phosphatidylinositol 3-kinase (PI3K) signaling pathway is frequently deregulated in cancer. Downstream of PI3K, Akt1 and Akt2 have opposing roles in breast cancer invasive migration, leading to metastatic dissemination. Here, we identify palladin, an actin-associated protein, as an Akt1-specific substrate that modulates breast cancer cell invasive migration. Akt1, but not Akt2, phosphorylates palladin at Ser507 in a domain that is critical for F-actin bundling. Downregulation of palladin enhances migration and invasion of breast cancer cells and induces abnormal branching morphogenesis in 3D cultures. Palladin phosphorylation at Ser507 is required for Akt1-mediated inhibition of breast cancer cell migration and also for F-actin bundling, leading to the maintenance of an organized actin cytoskeleton. These findings identify palladin as an Akt1-specific substrate that regulates cell motility and provide a molecular mechanism that accounts for the functional distinction between Akt isoforms in breast cancer cell signaling to cell migration.
journal_name
Mol Celljournal_title
Molecular cellauthors
Chin YR,Toker Adoi
10.1016/j.molcel.2010.02.031subject
Has Abstractpub_date
2010-05-14 00:00:00pages
333-44issue
3eissn
1097-2765issn
1097-4164pii
S1097-2765(10)00248-0journal_volume
38pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract::While much research has examined the use of glucose and glutamine by tumor cells, many cancers instead prefer to metabolize fats. Despite the pervasiveness of this phenotype, knowledge of pathways that drive fatty acid oxidation (FAO) in cancer is limited. Prolyl hydroxylase domain proteins hydroxylate substrate proli...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.08.014
更新日期:2016-09-15 00:00:00
abstract::Autophagic turnover of intracellular constituents is critical for cellular housekeeping, nutrient recycling, and various aspects of growth and development in eukaryotes. Here we show that autophagy impacts the other major degradative route involving the ubiquitin-proteasome system by eliminating 26S proteasomes, a pro...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2015.04.023
更新日期:2015-06-18 00:00:00
abstract::tRNAs reading four-codon families often have a modified uridine, cmo(5)U(34), at the wobble position of the anticodon. Here, we examine the effects on the decoding mechanism of a cmo(5)U modification in tRNA(1B)(Ala), anticodon C(36)G(35)cmo(5)U(34). tRNA(1B)(Ala) reads its cognate codons in a manner that is very simi...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.11.014
更新日期:2007-01-12 00:00:00
abstract::A truly groundbreaking paper by Pisarev et al. (2007), recently published in Cell, has solved a wide gap in our knowledge of eukaryotic mRNA translation, by elucidating how ribosomes are released from mRNA after the termination step. ...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2007.10.014
更新日期:2007-11-09 00:00:00
abstract::Anti-sense transcription originating upstream of mammalian protein-coding genes is a well-documented phenomenon, but remarkably little is known about the regulation or function of anti-sense promoters and the non-coding RNAs they generate. Here we define at nucleotide resolution the divergent transcription start sites...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2015.04.006
更新日期:2015-06-18 00:00:00
abstract::Glycogen is the major mammalian glucose storage cache and is critical for energy homeostasis. Glycogen synthesis in neurons must be tightly controlled due to neuronal sensitivity to perturbations in glycogen metabolism. Lafora disease (LD) is a fatal, congenital, neurodegenerative epilepsy. Mutations in the gene encod...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2014.11.020
更新日期:2015-01-22 00:00:00
abstract::The activation of Rab GTPases is a critical focal point of membrane trafficking events in eukaryotic cells; however, the cellular mechanisms that spatially and temporally regulate this process are poorly understood. Here, we identify a null allele of ELP1 as a suppressor of a mutant in a Rab guanine nucleotide exchang...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.02.018
更新日期:2005-03-18 00:00:00
abstract::CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreign-derived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. M...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.12.015
更新日期:2019-02-21 00:00:00
abstract::The ability to measure human aging from molecular profiles has practical implications in many fields, including disease prevention and treatment, forensics, and extension of life. Although chronological age has been linked to changes in DNA methylation, the methylome has not yet been used to measure and compare human ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2012.10.016
更新日期:2013-01-24 00:00:00
abstract::Type II topoisomerases are essential for resolving topologically entwined double-stranded DNA. Although anti-topoisomerase 2 (Top2) drugs are clinically important antibiotics and chemotherapies, to our knowledge, the mechanisms of cell killing by Top2 depletion and inactivation have never been directly compared. We sh...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2008.04.019
更新日期:2008-06-20 00:00:00
abstract::Faithful propagation of specific chromatin states requires re-establishment of epigenetic marks after every cell division. How the original epigenetic signature is inherited after disruption during DNA replication is still poorly understood. Here, we show that the poly(ADP-ribose)-polymerase-1 (PARP1/ARTD1) is implica...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2012.01.024
更新日期:2012-03-30 00:00:00
abstract::How proteasomal ATPases stimulate the gate opening of 20S proteasomes is a longstanding question. In the September 7 issue of Molecular Cell, Smith et al. (2007) describe the conserved "HbYX" motif as the key to proteasome gate opening. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2007.09.001
更新日期:2007-09-21 00:00:00
abstract::The Mre11/Rad50/Nbs1 complex initiates double-strand break repair by homologous recombination (HR). Loss of Mre11 or its nuclease activity in mouse cells is known to cause genome aberrations and cellular senescence, although the molecular basis for this phenotype is not clear. To identify the origin of these defects, ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.10.011
更新日期:2016-11-03 00:00:00
abstract::Proteins with annealing activity are newly identified ATP-dependent motors that can rewind RPA-coated complementary single-stranded DNA bubbles. AH2 (annealing helicase 2, also named as ZRANB3) is the second protein with annealing activity, the function of which is still unknown. Here, we report that AH2 is recruited ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2012.05.025
更新日期:2012-08-10 00:00:00
abstract::Activation of NF-kappaB is regulated via numerous ubiquitin- and proteasome-mediated steps; an important one is processing of the precursor p105 to the p50 active subunit. The mechanisms involved are largely unknown, because this is an exceptional case where the ubiquitin system does not destroy its substrate complete...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2009.01.023
更新日期:2009-02-27 00:00:00
abstract::Faithful transmission of genomic information requires tight spatiotemporal regulation of DNA replication factors. In the licensing step of DNA replication, CDT-1 is loaded onto chromatin to subsequently promote the recruitment of additional replication factors, including CDC-45 and GINS. During the elongation step, th...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.08.028
更新日期:2011-10-07 00:00:00
abstract::The stress response in eukaryotic cells often inhibits translation initiation and leads to the formation of cytoplasmic RNA-protein complexes referred to as stress granules. Stress granules contain nontranslating mRNAs, translation initiation components, and many additional proteins affecting mRNA function. Stress gra...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2009.11.020
更新日期:2009-12-25 00:00:00
abstract::Nucleotide-binding site leucine-rich repeat (NLR) receptors perceive pathogen effectors and trigger plant immunity. However, the mechanisms underlying NLR-triggered defense responses remain obscure. The recently discovered Pigm locus in rice encodes a cluster of NLRs, including PigmR, which confers broad-spectrum resi...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2019.03.013
更新日期:2019-06-06 00:00:00
abstract::The functional significance of mono-, di-, and trimethylation of lysine residues within histone proteins remains unclear. Antibodies developed to selectively recognize each of these methylated states at histone H3 lysine 9 (H3 Lys9) demonstrated that mono- and dimethylation localized specifically to silent domains wit...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00479-9
更新日期:2003-12-01 00:00:00
abstract::The interaction of transcription factors with target genes is highly dynamic. Whether the dynamic nature of these interactions is merely an intrinsic property of transcription factors or serves a regulatory role is unknown. Here we have used single-cell fluorescence imaging combined with computational modeling and chr...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2008.04.021
更新日期:2008-05-23 00:00:00
abstract::Nuclear RNA interference (RNAi) pathways work together with histone modifications to regulate gene expression and enact an adaptive response to transposable RNA elements. In the germline, nuclear RNAi can lead to trans-generational epigenetic inheritance (TEI) of gene silencing. We identified and characterized a famil...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2020.04.007
更新日期:2020-06-04 00:00:00
abstract::In Drosophila, specification of embryonic terminal cells is controlled by the Torso receptor tyrosine kinase. Here, we analyze the molecular basis of positive (Y630) and negative (Y918) phosphotyrosine (pY) signaling sites on Torso. We find that the Drosophila homolog of RasGAP associates with pY918 and is a negative ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80287-7
更新日期:1998-12-01 00:00:00
abstract::We demonstrate that the major in vivo targets of brefeldin A (BFA) in the secretory pathway of budding yeast are the three members of the Sec7 domain family of ARF exchange factors: Gea1p and Gea2p (functionally interchangeable) and Sec7p. Specific residues within the Sec7 domain are important for BFA inhibition of AR...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80455-4
更新日期:1999-03-01 00:00:00
abstract::The control of mRNA stability plays a central role in regulating gene expression. In metazoans, the earliest stages of development are driven by maternally supplied mRNAs. The degradation of these maternal mRNAs is critical for promoting the maternal-to-zygotic transition of developmental programs, although the underl...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2016.02.027
更新日期:2016-03-17 00:00:00
abstract::The nucleotide (p)ppGpp mediates bacterial stress responses, but its targets and underlying mechanisms of action vary among bacterial species and remain incompletely understood. Here, we characterize the molecular interaction between (p)ppGpp and guanylate kinase (GMK), revealing the importance of this interaction in ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2014.12.037
更新日期:2015-02-19 00:00:00
abstract::The Mediator complex connects transcriptional activators bound at enhancers with RNA polymerase (Pol) II. demonstrate that Mediator also has an important role in transcriptional activation after recruitment of the Pol II machinery to promoters. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.molcel.2005.03.002
更新日期:2005-03-18 00:00:00
abstract::S(N)1-type alkylating agents that produce cytotoxic O(6)-methyl-G (O(6)-meG) DNA adducts induce cell cycle arrest and apoptosis in a manner requiring the DNA mismatch repair (MMR) proteins MutSalpha and MutLalpha. Here, we show that checkpoint signaling in response to DNA methylation occurs during S phase and requires...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.04.023
更新日期:2006-05-19 00:00:00
abstract::The oncogenic process often leads to a loss of normal telomere length control, usually as a result of activation of telomerase. Nevertheless, there are also telomerase-independent events that involve a Rad50-dependent recombination mechanism to maintain telomere length. Previous work has implicated the Rb family of pr...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.02.016
更新日期:2006-04-07 00:00:00
abstract::CCR4-NOT is a major effector complex in miRNA-mediated gene silencing. It is recruited to miRNA targets through interactions with tryptophan (W)-containing motifs in TNRC6/GW182 proteins and is required for both translational repression and degradation of miRNA targets. Here, we elucidate the structural basis for the ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2014.03.034
更新日期:2014-06-05 00:00:00
abstract::In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic macromolecular machine. U4/U6.U5 tri-snRNP is the largest and most conserved building block of the spliceosome. By 3D elect...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.08.021
更新日期:2006-10-20 00:00:00