Abstract：
:The interaction of transcription factors with target genes is highly dynamic. Whether the dynamic nature of these interactions is merely an intrinsic property of transcription factors or serves a regulatory role is unknown. Here we have used single-cell fluorescence imaging combined with computational modeling and chromatin immunoprecipitation to analyze transcription complex dynamics in gene regulation during the cell cycle in living cells. We demonstrate a link between the dynamics of RNA polymerase I (RNA Pol I) assembly and transcriptional output. We show that transcriptional upregulation is accompanied by prolonged retention of RNA Pol I components at the promoter, resulting in longer promoter dwell time, and an increase in the steady-state population of assembling polymerase. As a consequence, polymerase assembly efficiency and, ultimately, the rate of entry into processive elongation are elevated. Our results show that regulation of rDNA transcription in vivo occurs via modulation of the efficiency of transcription complex subunit capture and assembly.
journal_name
Mol Celljournal_title
Molecular cellauthors
Gorski SA,Snyder SK,John S,Grummt I,Misteli Tdoi
10.1016/j.molcel.2008.04.021subject
Has Abstractpub_date
2008-05-23 00:00:00pages
486-97issue
4eissn
1097-2765issn
1097-4164pii
S1097-2765(08)00323-7journal_volume
30pub_type
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