当前位置： SCI文献检索 > MOLECULAR CELL期刊下所有文献 > A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi.

A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi.

Abstract：

:Nuclear RNA interference (RNAi) pathways work together with histone modifications to regulate gene expression and enact an adaptive response to transposable RNA elements. In the germline, nuclear RNAi can lead to trans-generational epigenetic inheritance (TEI) of gene silencing. We identified and characterized a family of nuclear Argonaute-interacting proteins (ENRIs) that control the strength and target specificity of nuclear RNAi in C. elegans, ensuring faithful inheritance of epigenetic memories. ENRI-1/2 prevent misloading of the nuclear Argonaute NRDE-3 with small RNAs that normally effect maternal piRNAs, which prevents precocious nuclear translocation of NRDE-3 in the early embryo. Additionally, they are negative regulators of nuclear RNAi triggered from exogenous sources. Loss of ENRI-3, an unstable protein expressed mostly in the male germline, misdirects the RNAi response to transposable elements and impairs TEI. The ENRIs determine the potency and specificity of nuclear RNAi responses by gating small RNAs into specific nuclear Argonautes.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Lewis A,Berkyurek AC,Greiner A,Sawh AN,Vashisht A,Merrett S,Flamand MN,Wohlschlegel J,Sarov M,Miska EA,Duchaine TF

doi

10.1016/j.molcel.2020.04.007

subject

Has Abstract

pub_date

2020-06-04 00:00:00

pages

862-875.e8

issue

5

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(20)30233-1

journal_volume

78

pub_type

杂志文章

相关文献

MOLECULAR CELL文献大全
  • Nuclear myosin VI enhances RNA polymerase II-dependent transcription.

    abstract：:Myosin VI is the only myosin that moves toward the minus end of actin filaments, suggesting a unique biological function. Here, we show that myosin VI is present in the nucleus of mammalian cells where it colocalizes with newly transcribed mRNA and with RNA polymerase II (RNAPII) and is detected in the RNAPII complex....

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2006.07.005

    authors： Vreugde S,Ferrai C,Miluzio A,Hauben E,Marchisio PC,Crippa MP,Bussi M,Biffo S

    更新日期：2006-09-01 00:00:00

  • Proteomic discovery of cellular substrates of the ClpXP protease reveals five classes of ClpX-recognition signals.

    abstract：:ClpXP is a protease involved in DNA damage repair, stationary-phase gene expression, and ssrA-mediated protein quality control. To date, however, only a handful of ClpXP substrates have been identified. Using a tagged and inactive variant of ClpP, substrates of E. coli ClpXP were trapped in vivo, purified, and identif...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(03)00060-1

    authors： Flynn JM,Neher SB,Kim YI,Sauer RT,Baker TA

    更新日期：2003-03-01 00:00:00

  • Mind the Gapmer: Implications of Co-transcriptional Cleavage by Antisense Oligonucleotides.

    abstract：:While gapmers efficiently knock down as well as terminate transcription of nascent lncRNAs and mRNAs, Lee and Mendell (2020) and Lai et al. (2020) also demonstrate that Pol II termination is not observed with gapmers targeting the 3' terminal portions of the transcript. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2020.02.010

    authors： Maranon DG,Wilusz J

    更新日期：2020-03-05 00:00:00

  • Srs2 disassembles Rad51 filaments by a protein-protein interaction triggering ATP turnover and dissociation of Rad51 from DNA.

    abstract：:Rad51 is a DNA recombinase functioning in the repair of DNA double-strand breaks and the generation of genetic diversity by homologous recombination (HR). In the presence of ATP, Rad51 self-assembles into an extended polymer on single-stranded DNA to catalyze strand exchange. Inappropriate HR causes genomic instabilit...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.05.026

    authors： Antony E,Tomko EJ,Xiao Q,Krejci L,Lohman TM,Ellenberger T

    更新日期：2009-07-10 00:00:00

  • TORC2 signaling pathway guarantees genome stability in the face of DNA strand breaks.

    abstract：:A chemicogenetic screen was performed in budding yeast mutants that have a weakened replication stress response. This identified an inhibitor of target of rapamycin (TOR) complexes 1 and 2 that selectively enhances the sensitivity of sgs1Δ cells to hydroxyurea and camptothecin. More importantly, the inhibitor has stro...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2013.08.019

    authors： Shimada K,Filipuzzi I,Stahl M,Helliwell SB,Studer C,Hoepfner D,Seeber A,Loewith R,Movva NR,Gasser SM

    更新日期：2013-09-26 00:00:00

  • Structure of the papillomavirus DNA-tethering complex E2:Brd4 and a peptide that ablates HPV chromosomal association.

    abstract：:Many DNA viruses that are latent in dividing cells are noncovalent passengers on mitotic chromosomes and require specific viral-encoded and cellular factors for this activity. The chromosomal protein Brd4 is implicated in the hitchhiking of bovine papillomavirus-1 (BPV-1), and the viral protein E2 binds to both plasmi...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2006.11.002

    authors： Abbate EA,Voitenleitner C,Botchan MR

    更新日期：2006-12-28 00:00:00

  • Monitoring homology search during DNA double-strand break repair in vivo.

    abstract：:Homologous recombination (HR) is crucial for genetic exchange and accurate repair of DNA double-strand breaks and is pivotal for genome integrity. HR uses homologous sequences for repair, but how homology search, the exploration of the genome for homologous DNA sequences, is conducted in the nucleus remains poorly und...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2013.02.020

    authors： Renkawitz J,Lademann CA,Kalocsay M,Jentsch S

    更新日期：2013-04-25 00:00:00

  • Guide RNA functional modules direct Cas9 activity and orthogonality.

    abstract：:The RNA-guided Cas9 endonuclease specifically targets and cleaves DNA in a sequence-dependent manner and has been widely used for programmable genome editing. Cas9 activity is dependent on interactions with guide RNAs, and evolutionarily divergent Cas9 nucleases have been shown to work orthogonally. However, the molec...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.09.019

    authors： Briner AE,Donohoue PD,Gomaa AA,Selle K,Slorach EM,Nye CH,Haurwitz RE,Beisel CL,May AP,Barrangou R

    更新日期：2014-10-23 00:00:00

  • Promoter Distortion and Opening in the RNA Polymerase II Cleft.

    abstract：:Transcription initiation requires opening of promoter DNA in the RNA polymerase II (Pol II) pre-initiation complex (PIC), but it remains unclear how this is achieved. Here we report the cryo-electron microscopic (cryo-EM) structure of a yeast PIC that contains underwound, distorted promoter DNA in the closed Pol II cl...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.10.014

    authors： Dienemann C,Schwalb B,Schilbach S,Cramer P

    更新日期：2019-01-03 00:00:00

  • Essential Roles of the Smc5/6 Complex in Replication through Natural Pausing Sites and Endogenous DNA Damage Tolerance.

    abstract：:The essential functions of the conserved Smc5/6 complex remain elusive. To uncover its roles in genome maintenance, we established Saccharomyces cerevisiae cell-cycle-regulated alleles that enable restriction of Smc5/6 components to S or G2/M. Unexpectedly, the essential functions of Smc5/6 segregated fully and select...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2015.10.023

    authors： Menolfi D,Delamarre A,Lengronne A,Pasero P,Branzei D

    更新日期：2015-12-17 00:00:00

  • The N-terminal domain of Nup159 forms a beta-propeller that functions in mRNA export by tethering the helicase Dbp5 to the nuclear pore.

    abstract：:Nuclear export of mRNA in eukaryotic cells is mediated by soluble transport factors and components of the nuclear pore complex (NPC). The cytoplasmically oriented nuclear pore protein Nup159 plays a critical role in mRNA export through its conserved N-terminal domain (NTD). Here, we report the crystal structure of the...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2004.10.032

    authors： Weirich CS,Erzberger JP,Berger JM,Weis K

    更新日期：2004-12-03 00:00:00

  • Autoinhibition of Bcr-Abl through its SH3 domain.

    abstract：:Bcr-Abl is a dysregulated tyrosine kinase whose mechanism of activation is unclear. Here, we demonstrate that, like c-Abl, Bcr-Abl is negatively regulated through its SH3 domain. Kinase activity, transformation, and leukemogenesis by Bcr-Abl are greatly impaired by mutations of the Bcr coiled-coil domain that disrupt ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(03)00274-0

    authors： Smith KM,Yacobi R,Van Etten RA

    更新日期：2003-07-01 00:00:00

  • Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs.

    abstract：:Upon glucose restriction, eukaryotic cells upregulate oxidative metabolism to maintain homeostasis. Using genetic screens, we find that the mitochondrial serine hydroxymethyltransferase (SHMT2) is required for robust mitochondrial oxygen consumption and low glucose proliferation. SHMT2 catalyzes the first step in mito...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.01.024

    authors： Minton DR,Nam M,McLaughlin DJ,Shin J,Bayraktar EC,Alvarez SW,Sviderskiy VO,Papagiannakopoulos T,Sabatini DM,Birsoy K,Possemato R

    更新日期：2018-02-15 00:00:00

  • Involvement of actin-related proteins in ATP-dependent chromatin remodeling.

    abstract：:Actin-related proteins (Arps) and conventional actin are enigmatic components of many chromatin-remodeling enzyme complexes. The yeast INO80 ATP-dependent chromatin-remodeling complex contains stoichiometric amounts of Arp4, Arp5, Arp8, and actin. Here we have revealed functions of Arp5 and Arp8 by analysis of mutants...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(03)00264-8

    authors： Shen X,Ranallo R,Choi E,Wu C

    更新日期：2003-07-01 00:00:00

  • Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity.

    abstract：:We have analyzed two residues in the helicase domain of the E1 initiator protein. These residues are part of a highly conserved structural motif, the beta-hairpin, which is present in the helicase domain of all papovavirus initiator proteins. These proteins are unique in their ability to transition from local template...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2007.02.009

    authors： Liu X,Schuck S,Stenlund A

    更新日期：2007-03-23 00:00:00

  • Phosphorylation of LSD1 by PKCα is crucial for circadian rhythmicity and phase resetting.

    abstract：:The circadian clock is a self-sustaining oscillator that controls daily rhythms. For the proper circadian gene expression, dynamic changes in chromatin structure are important. Although chromatin modifiers have been shown to play a role in circadian gene expression, the in vivo role of circadian signal-modulated chrom...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.01.028

    authors： Nam HJ,Boo K,Kim D,Han DH,Choe HK,Kim CR,Sun W,Kim H,Kim K,Lee H,Metzger E,Schuele R,Yoo SH,Takahashi JS,Cho S,Son GH,Baek SH

    更新日期：2014-03-06 00:00:00

  • Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells.

    abstract：:Eukaryotic cells compartmentalize biochemical processes in different organelles, often relying on metabolic cycles to shuttle reducing equivalents across intracellular membranes. NADPH serves as the electron carrier for the maintenance of redox homeostasis and reductive biosynthesis, with separate cytosolic and mitoch...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2014.05.008

    authors： Lewis CA,Parker SJ,Fiske BP,McCloskey D,Gui DY,Green CR,Vokes NI,Feist AM,Vander Heiden MG,Metallo CM

    更新日期：2014-07-17 00:00:00

  • The structure of a receptor with two associating transmembrane domains on the cell surface: integrin alphaIIbbeta3.

    abstract：:Structures of intact receptors with single-pass transmembrane domains are essential to understand how extracellular and cytoplasmic domains regulate association and signaling through transmembrane domains. A chemical and computational method to determine structures of the membrane regions of such receptors on the cell...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.02.022

    authors： Zhu J,Luo BH,Barth P,Schonbrun J,Baker D,Springer TA

    更新日期：2009-04-24 00:00:00

  • Caspase-2-mediated cleavage of Mdm2 creates a p53-induced positive feedback loop.

    abstract：:Caspase-2 is an evolutionarily conserved caspase, yet its biological function and cleavage targets are poorly understood. Caspase-2 is activated by the p53 target gene product PIDD (also known as LRDD) in a complex called the Caspase-2-PIDDosome. We show that PIDD expression promotes growth arrest and chemotherapy res...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2011.06.012

    authors： Oliver TG,Meylan E,Chang GP,Xue W,Burke JR,Humpton TJ,Hubbard D,Bhutkar A,Jacks T

    更新日期：2011-07-08 00:00:00

  • The three Rs of transcription: recruit, retain, and recycle.

    abstract：:The dynamic protein interactions required for transcription are functionally important yet poorly understood; in this issue of Molecular Cell, Zobeck et al. (2010) resolve the sequential recruitment and selective recycling of transcription factors at an actively transcribing locus in Drosophila. ...

    journal_title：Molecular cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.molcel.2010.12.010

    authors： Motta-Mena LB,Partch CL,Gardner KH

    更新日期：2010-12-22 00:00:00

  • ATR kinase activation mediated by MutSalpha and MutLalpha in response to cytotoxic O6-methylguanine adducts.

    abstract：:S(N)1-type alkylating agents that produce cytotoxic O(6)-methyl-G (O(6)-meG) DNA adducts induce cell cycle arrest and apoptosis in a manner requiring the DNA mismatch repair (MMR) proteins MutSalpha and MutLalpha. Here, we show that checkpoint signaling in response to DNA methylation occurs during S phase and requires...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2006.04.023

    authors： Yoshioka K,Yoshioka Y,Hsieh P

    更新日期：2006-05-19 00:00:00

  • Rad54 functions as a heteroduplex DNA pump modulated by its DNA substrates and Rad51 during D loop formation.

    abstract：:The displacement loop (D loop) is the product of homology search and DNA strand invasion, constituting a central intermediate in homologous recombination (HR). In eukaryotes, the Rad51 DNA strand exchange protein is assisted in D loop formation by the Rad54 motor protein. Curiously, Rad54 also disrupts D loops. How th...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2013.12.027

    authors： Wright WD,Heyer WD

    更新日期：2014-02-06 00:00:00

  • Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a.

    abstract：:CRISPR-Cas12a (Cpf1) is an RNA-guided DNA-cutting nuclease that has been repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target DNA in cis and non-target single-stranded DNAs (ssDNAs) in trans. To elucidate the molecular basis for both DNase cleavage modes, we performed structural and b...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2018.11.021

    authors： Swarts DC,Jinek M

    更新日期：2019-02-07 00:00:00

  • Mechanism of homology recognition in DNA recombination from dual-molecule experiments.

    abstract：:In E. coli homologous recombination, a filament of RecA protein formed on DNA searches and pairs a homologous sequence within a second DNA molecule with remarkable speed and fidelity. Here, we directly probe the strength of the two-molecule interactions involved in homology search and recognition using dual-molecule m...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2012.03.029

    authors： De Vlaminck I,van Loenhout MT,Zweifel L,den Blanken J,Hooning K,Hage S,Kerssemakers J,Dekker C

    更新日期：2012-06-08 00:00:00

  • GPS2 is required for cholesterol efflux by triggering histone demethylation, LXR recruitment, and coregulator assembly at the ABCG1 locus.

    abstract：:Transcriptional coregulators, rather than ligand signals, are suspected to confer context and pathway specificity to nuclear receptor signaling, but the identity of such specifying coregulators and the underlying molecular mechanisms remain largely enigmatic. Here we address this issue in metabolic oxysterol receptor ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.05.006

    authors： Jakobsson T,Venteclef N,Toresson G,Damdimopoulos AE,Ehrlund A,Lou X,Sanyal S,Steffensen KR,Gustafsson JA,Treuter E

    更新日期：2009-05-14 00:00:00

  • Type IV pilin structure and assembly: X-ray and EM analyses of Vibrio cholerae toxin-coregulated pilus and Pseudomonas aeruginosa PAK pilin.

    abstract：:Pilin assembly into type IV pili is required for virulence by bacterial pathogens that cause diseases such as cholera, pneumonia, gonorrhea, and meningitis. Crystal structures of soluble, N-terminally truncated pilin from Vibrio cholera toxin-coregulated pilus (TCP) and full-length PAK pilin from Pseudomonas aeruginos...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(03)00170-9

    authors： Craig L,Taylor RK,Pique ME,Adair BD,Arvai AS,Singh M,Lloyd SJ,Shin DS,Getzoff ED,Yeager M,Forest KT,Tainer JA

    更新日期：2003-05-01 00:00:00

  • A Conserved Kinase-Based Body-Temperature Sensor Globally Controls Alternative Splicing and Gene Expression.

    abstract：:Homeothermic organisms maintain their core body temperature in a narrow, tightly controlled range. Whether and how subtle circadian oscillations or disease-associated changes in core body temperature are sensed and integrated in gene expression programs remain elusive. Furthermore, a thermo-sensor capable of sensing t...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2020.01.028

    authors： Haltenhof T,Kotte A,De Bortoli F,Schiefer S,Meinke S,Emmerichs AK,Petermann KK,Timmermann B,Imhof P,Franz A,Loll B,Wahl MC,Preußner M,Heyd F

    更新日期：2020-04-02 00:00:00

  • Oligomeric initiator protein-mediated DNA looping negatively regulates plasmid replication in vitro by preventing origin melting.

    abstract：:Although DNA looping between the initiator binding sites (iterons) of the replication origin (ori) of a plasmid and the iterons located in a cis-acting control sequence called inc has been postulated to promote negative control of plasmid DNA replication, not only was definitive evidence for such looping lacking, but ...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2005.10.037

    authors： Zzaman S,Bastia D

    更新日期：2005-12-22 00:00:00

  • Sumoylation of RecQ helicase controls the fate of dysfunctional telomeres.

    abstract：:Genome stability depends upon the RecQ helicases, which are conserved from bacteria to man, but little is known about how their myriad activities are regulated. Fission yeast lacking the telomere protein Taz1 (mammalian TRF1/TRF2 ortholog) lose many hallmarks of telomeres, including accurate replication and local prot...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/j.molcel.2009.01.027

    authors： Rog O,Miller KM,Ferreira MG,Cooper JP

    更新日期：2009-03-13 00:00:00

  • Chromatin remodeling in vivo: evidence for a nucleosome sliding mechanism.

    abstract：:Members of the ISWI family of chromatin remodeling factors exhibit ATP-dependent nucleosome sliding, loading, and spacing activities in vitro. However, it is unclear which of these activities are utilized by ISWI complexes to remodel chromatin in vivo. We therefore sought to identify the mechanisms of chromatin remode...

    journal_title：Molecular cell

    pub_type： 杂志文章

    doi：10.1016/s1097-2765(03)00436-2

    authors： Fazzio TG,Tsukiyama T

    更新日期：2003-11-01 00:00:00