Transcriptional activation: mediator can act after preinitiation complex formation.

abstract：:In this issue of Molecular Cell, Huang et al. (2009) describe two heterotrimeric single-stranded DNA binding complexes, SOSS1 and SOSS2, that function downstream of the MRN complex to promote DNA repair and the G2/M checkpoint. ...

journal_title：Molecular cell

authors： Nam EA,Cortez D

更新日期：2009-08-14 00:00:00

abstract：:FMOC-L-Leucine (F-L-Leu) is a chemically distinct PPARgamma ligand. Two molecules of F-L-Leu bind to the ligand binding domain of a single PPARgamma molecule, making its mode of receptor interaction distinct from that of other nuclear receptor ligands. F-L-Leu induces a particular allosteric configuration of PPARgamma...

journal_title：Molecular cell

authors： Rocchi S,Picard F,Vamecq J,Gelman L,Potier N,Zeyer D,Dubuquoy L,Bac P,Champy MF,Plunket KD,Leesnitzer LM,Blanchard SG,Desreumaux P,Moras D,Renaud JP,Auwerx J

更新日期：2001-10-01 00:00:00

abstract：:In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic macromolecular machine. U4/U6.U5 tri-snRNP is the largest and most conserved building block of the spliceosome. By 3D elect...

journal_title：Molecular cell

authors： Sander B,Golas MM,Makarov EM,Brahms H,Kastner B,Lührmann R,Stark H

更新日期：2006-10-20 00:00:00

abstract：:Cohesion between sister chromatids is mediated by the chromosomal cohesin complex. In budding yeast, cohesin is loaded onto chromosomes during the G1 phase of the cell cycle. During S phase, the replication fork-associated acetyltransferase Eco1 acetylates the cohesin subunit Smc3 to promote the establishment of siste...

journal_title：Molecular cell

authors： Borges V,Lehane C,Lopez-Serra L,Flynn H,Skehel M,Rolef Ben-Shahar T,Uhlmann F

更新日期：2010-09-10 00:00:00

abstract：:Budding yeast Cdc13, Stn1, and Ten1 form the CST complex to protect telomeres from lethal DNA degradation. It remains unknown whether similar complexes are conserved in higher eukaryotes or not. Here we isolated mammalian STN1 and TEN1 homologs and CTC1 (conserved telomere maintenance component 1). The three proteins ...

journal_title：Molecular cell

authors： Miyake Y,Nakamura M,Nabetani A,Shimamura S,Tamura M,Yonehara S,Saito M,Ishikawa F

更新日期：2009-10-23 00:00:00

abstract：:The abundant chromatin-associated human factor HCF-1 is a heterodimeric complex of HCF-1N and HCF-1C subunits that are essential for two stages of the cell cycle. The HCF-1N subunit promotes G1 phase progression, whereas the HCF-1C subunit ensures proper cytokinesis at completion of M phase. How the HCF-1C subunit fun...

journal_title：Molecular cell

authors： Julien E,Herr W

更新日期：2004-06-18 00:00:00

abstract：:Activating mutations in NOTCH1, an essential regulator of T cell development, are frequently found in human T cell acute lymphoblastic leukemia (T-ALL). Despite important advances in our understanding of Notch signal transduction, the regulation of Notch functions in the nucleus remains unclear. Using immunoaffinity p...

journal_title：Molecular cell

authors： Yatim A,Benne C,Sobhian B,Laurent-Chabalier S,Deas O,Judde JG,Lelievre JD,Levy Y,Benkirane M

更新日期：2012-11-09 00:00:00

abstract：:The Cdc28p cyclin-dependent kinase is thought to both catalyze the onset of DNA replication and prevent rereplication by blocking the reassembly of initiation complexes at replication origins. Budding yeast with mutations in the CDC16 gene represent an exception to this model, because they rereplicate DNA despite bein...

journal_title：Molecular cell

authors： Heichman KA,Roberts JM

更新日期：1998-02-01 00:00:00

abstract：:Faithful copying of the genome is essential for life. In eukaryotes, a single archaeo-eukaryotic primase (AEP), DNA primase, is required for the initiation and progression of DNA replication. Here we have identified additional eukaryotic AEP-like proteins with DNA-dependent primase and/or polymerase activity. Uniquely...

journal_title：Molecular cell

authors： Rudd SG,Glover L,Jozwiakowski SK,Horn D,Doherty AJ

更新日期：2013-11-21 00:00:00

abstract：:In eukaryotes, the 20S proteasome contains two chymotrypsin-like, two trypsin-like, and two active sites shown here to have caspase-like specificity. We report that certain sites allosterically regulate each other's activities. Substrates of a chymotrypsin-like site stimulate dramatically the caspase-like activity and...

journal_title：Molecular cell

authors： Kisselev AF,Akopian TN,Castillo V,Goldberg AL

更新日期：1999-09-01 00:00:00

abstract：:For single-cell organisms, nutrient uptake and metabolism are central to the fundamental decision of whether to grow or divide. In metazoans, cell fate decisions are more complex: organismal homeostasis must be strictly maintained by balancing cell proliferation and death. Despite this increased complexity, cell fate ...

journal_title：Molecular cell

authors： Andersen JL,Kornbluth S

更新日期：2013-02-07 00:00:00

abstract：:R loop, a transcription intermediate containing RNA:DNA hybrids and displaced single-stranded DNA (ssDNA), has emerged as a major source of genomic instability. RNaseH1, which cleaves the RNA in RNA:DNA hybrids, plays an important role in R loop suppression. Here we show that replication protein A (RPA), an ssDNA-bind...

journal_title：Molecular cell

authors： Nguyen HD,Yadav T,Giri S,Saez B,Graubert TA,Zou L

更新日期：2017-03-02 00:00:00

abstract：:As solid tumors expand, oxygen and nutrients become limiting owing to inadequate vascularization and diffusion. How malignant cells cope with this potentially lethal metabolic stress remains poorly understood. We found that glucose shortage associated with malignant progression triggers apoptosis through the endoplasm...

journal_title：Molecular cell

authors： Huber AL,Lebeau J,Guillaumot P,Pétrilli V,Malek M,Chilloux J,Fauvet F,Payen L,Kfoury A,Renno T,Chevet E,Manié SN

更新日期：2013-03-28 00:00:00

abstract：:In this issue of Molecular Cell, Weinert et al. (2013) demonstrate that the intermediary metabolite acetyl-phosphate is an important acetyl donor that contributes to global protein acetylation in growth-arrested E. coli. ...

journal_title：Molecular cell

authors： Verdin E,Ott M

更新日期：2013-07-25 00:00:00

abstract：:The structure of an RNA polymerase II/general transcription factor TFIIF complex was determined by cryo-electron microscopy and single particle analysis. Density due to TFIIF was not concentrated in one area but rather was widely distributed across the surface of the polymerase. The largest subunit of TFIIF interacted...

journal_title：Molecular cell

authors： Chung WH,Craighead JL,Chang WH,Ezeokonkwo C,Bareket-Samish A,Kornberg RD,Asturias FJ

更新日期：2003-10-01 00:00:00

abstract：:An important paper by David Allis and colleagues (Ahn et al., 2006 [this issue of Molecular Cell]) describes a specific deacetylation/phosphorylation crosstalk at the histone H2B tail required for apoptosis induction in yeast, thus giving first insights into the poorly understood epigenetic regulation of cell death. ...

journal_title：Molecular cell

authors： Carmona-Gutierrez D,Madeo F

更新日期：2006-10-20 00:00:00

abstract：:The Drosophila gene vasa (vas) encodes an RNA-binding protein required for embryonic patterning and germ cell specification. In vas mutants, translation of several germline mRNAs is reduced. Here we show that VAS interacts directly with the Drosophila homolog of yeast translation initiation factor 2, encoded by a nove...

journal_title：Molecular cell

authors： Carrera P,Johnstone O,Nakamura A,Casanova J,Jäckle H,Lasko P

更新日期：2000-01-01 00:00:00

abstract：:S(N)1-type alkylating agents that produce cytotoxic O(6)-methyl-G (O(6)-meG) DNA adducts induce cell cycle arrest and apoptosis in a manner requiring the DNA mismatch repair (MMR) proteins MutSalpha and MutLalpha. Here, we show that checkpoint signaling in response to DNA methylation occurs during S phase and requires...

journal_title：Molecular cell

authors： Yoshioka K,Yoshioka Y,Hsieh P

更新日期：2006-05-19 00:00:00

abstract：:In this issue, He et al. (2015) show how herpes virus usurps a cellular metabolic enzyme to induce RIG-I deamidation and RNA-independent activation, likely to better prevent further innate immune responses. ...

journal_title：Molecular cell

authors： Kolakofsky D,Garcin D

更新日期：2015-04-02 00:00:00

abstract：:Mammalian target of rapamycin (mTOR) activity is regulated by assembly of two functionally distinct complexes, mTORC1 and mTORC2. In syndecan-4 (S4) null endothelial cells, mTORC2 activity is reduced, resulting in decreased Akt activation, while mTORC1 activity is increased. Levels of rictor, mLST8, and mSin-1 are unc...

journal_title：Molecular cell

authors： Partovian C,Ju R,Zhuang ZW,Martin KA,Simons M

更新日期：2008-10-10 00:00:00

abstract：:Vascular endothelial (VE)-cadherin homophilic adhesion controls endothelial barrier permeability through assembly of adherens junctions (AJs). We observed that loss of VE-cadherin-mediated adhesion induced the activation of Src and phospholipase C (PLC)γ2, which mediated Ca(2+) release from endoplasmic reticulum (ER) ...

journal_title：Molecular cell

authors： Komarova YA,Huang F,Geyer M,Daneshjou N,Garcia A,Idalino L,Kreutz B,Mehta D,Malik AB

更新日期：2012-12-28 00:00:00

abstract：:In two recent publications in Molecular Cell,Boulias et al. (2019) and Sendinc et al. (2019) use complementary approaches to map m6Am modification sites transcriptome-wide and demonstrate that m6Am can repress translation while increasing the stability of a subset of low-abundance transcripts. ...

journal_title：Molecular cell

authors： Doxtader KA,Nam Y

更新日期：2019-08-08 00:00:00

abstract：:The RNA-guided Cas9 endonuclease specifically targets and cleaves DNA in a sequence-dependent manner and has been widely used for programmable genome editing. Cas9 activity is dependent on interactions with guide RNAs, and evolutionarily divergent Cas9 nucleases have been shown to work orthogonally. However, the molec...

journal_title：Molecular cell

authors： Briner AE,Donohoue PD,Gomaa AA,Selle K,Slorach EM,Nye CH,Haurwitz RE,Beisel CL,May AP,Barrangou R

更新日期：2014-10-23 00:00:00

abstract：:Superfamily ATPases in type IV pili, type 2 secretion, and archaella (formerly archaeal flagella) employ similar sequences for distinct biological processes. Here, we structurally and functionally characterize prototypical superfamily ATPase FlaI in Sulfolobus acidocaldarius, showing FlaI activities in archaeal swimmi...

journal_title：Molecular cell

authors： Reindl S,Ghosh A,Williams GJ,Lassak K,Neiner T,Henche AL,Albers SV,Tainer JA

更新日期：2013-03-28 00:00:00

abstract：:The formation of silenced and condensed heterochromatin foci involves enrichment of heterochromatin protein 1 (HP1). HP1 can bridge chromatin segments and form liquid droplets, but the biophysical principles underlying heterochromatin compartmentalization in the cell nucleus are elusive. Here, we assess mechanisticall...

journal_title：Molecular cell

authors： Erdel F,Rademacher A,Vlijm R,Tünnermann J,Frank L,Weinmann R,Schweigert E,Yserentant K,Hummert J,Bauer C,Schumacher S,Al Alwash A,Normand C,Herten DP,Engelhardt J,Rippe K

更新日期：2020-04-16 00:00:00

abstract：:Chaperone-mediated autophagy (CMA) is involved in cellular quality control, and in the response to stress, deterioration in CMA contributes to the aging phenotype and to various disorders. The paper by Bandyopadhyay et al. in this issue of Molecular Cell demonstrates the role of GTP binding by elongation factor 1 alph...

journal_title：Molecular cell

authors： Beau I,Codogno P

更新日期：2010-08-27 00:00:00

abstract：:Nine human neurodegenerative diseases are due to expansion of a CAG repeat- encoding glutamine within the open reading frame of the respective genes. Polyglutamine (polyQ) expansion confers dominant toxicity, resulting in neuronal degeneration. MicroRNAs (miRNAs) have been shown to modulate programmed cell death durin...

journal_title：Molecular cell

authors： Bilen J,Liu N,Burnett BG,Pittman RN,Bonini NM

更新日期：2006-10-06 00:00:00

abstract：:Cellular RNAs can be chemically modified over a hundred different ways. These modifications were once thought to be static, discrete, and utilized to fine-tune RNA structure and function. However, recent studies have revealed that some modifications, like mRNA methylation, can be reversed, and these reversible modific...

journal_title：Molecular cell

authors： Wang X,He C

更新日期：2014-10-02 00:00:00

abstract：:Many genomic studies have revealed associations between the gut microbiota composition and host metabolism. These observations led to the idea that a causal relationship could exist between the microbiota and metabolic diseases, a concept supported by studies showing compositional changes in the microbial community in...

journal_title：Molecular cell

authors： Koh A,Bäckhed F

更新日期：2020-05-21 00:00:00

abstract：:X chromosome inactivation (XCI) depends on a noncoding sense-antisense transcript pair, Xist and Tsix. At the onset of XCI, Xist RNA accumulates on one of two Xs, coating and silencing the chromosome in cis. The molecular basis for monoallelic Xist upregulation is not known, though evidence predominantly supports a po...

journal_title：Molecular cell

authors： Sun BK,Deaton AM,Lee JT

更新日期：2006-03-03 00:00:00