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Insights into FlaI functions in archaeal motor assembly and motility from structures, conformations, and genetics.

Abstract：

:Superfamily ATPases in type IV pili, type 2 secretion, and archaella (formerly archaeal flagella) employ similar sequences for distinct biological processes. Here, we structurally and functionally characterize prototypical superfamily ATPase FlaI in Sulfolobus acidocaldarius, showing FlaI activities in archaeal swimming-organelle assembly and movement. X-ray scattering data of FlaI in solution and crystal structures with and without nucleotide reveal a hexameric crown assembly with key cross-subunit interactions. Rigid building blocks form between N-terminal domains (points) and neighboring subunit C-terminal domains (crown ring). Upon nucleotide binding, these six cross-subunit blocks move with respect to each other and distinctly from secretion and pilus ATPases. Crown interactions and conformations regulate assembly, motility, and force direction via a basic-clamp switching mechanism driving conformational changes between stable, backbone-interconnected moving blocks. Collective structural and mutational results identify in vivo functional components for assembly and motility, phosphate-triggered rearrangements by ATP hydrolysis, and molecular predictors for distinct ATPase superfamily functions.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Reindl S,Ghosh A,Williams GJ,Lassak K,Neiner T,Henche AL,Albers SV,Tainer JA

doi

10.1016/j.molcel.2013.01.014

subject

Has Abstract

pub_date

2013-03-28 00:00:00

pages

1069-82

issue

6

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(13)00047-6

journal_volume

49

pub_type

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