Abstract:
:In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic macromolecular machine. U4/U6.U5 tri-snRNP is the largest and most conserved building block of the spliceosome. By 3D electron cryomicroscopy and labeling, the exon-aligning U5 snRNA loop I is localized at the center of the tetrahedrally shaped tri-snRNP reconstructed to approximately 2.1 nm resolution in vitrified ice. Independent 3D reconstructions of its subunits, U4/U6 and U5 snRNPs, show how U4/U6 and U5 combine to form tri-snRNP and, together with labeling experiments, indicate a close proximity of the spliceosomal core components U5 snRNA loop I and U4/U6 at the center of tri-snRNP. We suggest that this central tri-snRNP region may be the site to which the prespliceosomal U2 snRNA has to approach closely during formation of the catalytic core of the spliceosome.
journal_name
Mol Celljournal_title
Molecular cellauthors
Sander B,Golas MM,Makarov EM,Brahms H,Kastner B,Lührmann R,Stark Hdoi
10.1016/j.molcel.2006.08.021subject
Has Abstractpub_date
2006-10-20 00:00:00pages
267-78issue
2eissn
1097-2765issn
1097-4164pii
S1097-2765(06)00603-4journal_volume
24pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract::We generated knockout mice for MCM8 and MCM9 and show that deficiency for these genes impairs homologous recombination (HR)-mediated DNA repair during gametogenesis and somatic cells cycles. MCM8(-/-) mice are sterile because spermatocytes are blocked in meiotic prophase I, and females have only arrested primary folli...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2012.05.048
更新日期:2012-08-24 00:00:00
abstract::The ribosome, a ribonucleoprotein machine for protein synthesis, can also serve as an abundant nutrient source under starvation conditions. In a recent issue of Science, Wyant et al. (2018) discovered a specialized "spoon" to "scoop up" more ribosomes for degradation by autophagy. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2018.07.003
更新日期:2018-07-19 00:00:00
abstract::X chromosome inactivation (XCI) depends on a noncoding sense-antisense transcript pair, Xist and Tsix. At the onset of XCI, Xist RNA accumulates on one of two Xs, coating and silencing the chromosome in cis. The molecular basis for monoallelic Xist upregulation is not known, though evidence predominantly supports a po...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.01.028
更新日期:2006-03-03 00:00:00
abstract::PRDM9 determines the localization of meiotic recombination hotspots, which are associated with histone H3 methylation. It is not known whether PRDM9's methyltransferase activity is required or how some PRDM9 alleles can dominate the distribution of hotspots over other alleles. Diagouraga, Clément, and colleagues (2018...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2018.02.021
更新日期:2018-03-01 00:00:00
abstract::Recent work from Kuehner and Brow (2008) and Thiebaut et al. (2008) in Molecular Cell and Jenks et al. (2008) in Molecular and Cellular Biology reveals that regulated expression of central nucleotide synthesis pathway components directs start site-dependent RNA polymerase II termination. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2008.08.014
更新日期:2008-09-05 00:00:00
abstract::The mechanism by which (CTG)n expansion in the 3' UTR of the DMPK gene causes myotonic dystrophy (DM) is unknown. We identified four RNA splicing factors--hnRNP C, U2AF (U2 auxiliary factor), PTB (polypyrimidine tract binding protein), and PSF (PTB associated splicing factor)--that bind to two short regions 3' of the ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80261-0
更新日期:2000-06-01 00:00:00
abstract::Mutations within BRCA1 predispose carriers to a high risk of breast and ovarian cancers. BRCA1 functions to maintain genomic stability through the assembly of multiple protein complexes involved in DNA repair, cell-cycle arrest, and transcriptional regulation. Here, we report the identification of a DNA damage-induced...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2014.03.021
更新日期:2014-05-08 00:00:00
abstract::Myosin VI is the only myosin that moves toward the minus end of actin filaments, suggesting a unique biological function. Here, we show that myosin VI is present in the nucleus of mammalian cells where it colocalizes with newly transcribed mRNA and with RNA polymerase II (RNAPII) and is detected in the RNAPII complex....
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2006.07.005
更新日期:2006-09-01 00:00:00
abstract::The disulfide relay system in the intermembrane space of mitochondria is of crucial importance for mitochondrial biogenesis. Major players in this pathway are the oxidoreductase Mia40 that oxidizes substrates and the sulfhydryl oxidase Erv1 that reoxidizes Mia40. To analyze in detail the mechanism of this oxidative pa...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2010.01.017
更新日期:2010-02-26 00:00:00
abstract::BIK protein is an initiator of mitochondrial apoptosis, and BIK expression is induced by proapoptotic signals, including DNA damage. Here, we demonstrate that 3' end processing and expression of BIK mRNA are controlled by the nuclear PI4,5P(2)-regulated poly(A) polymerase Star-PAP downstream of DNA damage. Nuclear PKC...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2011.11.017
更新日期:2012-01-13 00:00:00
abstract::In addition to its role as an electron transporter, mitochondrial nicotinamide adenine dinucleotide (NAD+) is an important co-factor for enzymatic reactions, including ADP-ribosylation. Although mitochondria harbor the most intra-cellular NAD+, mitochondrial ADP-ribosylation remains poorly understood. Here we provide ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2020.12.034
更新日期:2021-01-21 00:00:00
abstract::SNAP proteins play an essential role in membrane trafficking in eukaryotic cells. They activate and recycle SNARE proteins by serving as adaptors between SNAREs and the cytosolic chaperone NSF. We have determined the crystal structure of Sec17, the yeast homolog of alpha-SNAP, to 2.9 A resolution. Sec17 is composed of...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80190-2
更新日期:1999-07-01 00:00:00
abstract::One of the prominent biological phenomena controlled by Wnt signaling is the expansion of cells with predefined fates. In the November 27, 2002, issue of Cell, Rosenfeld and colleagues describe an example of Wnt-controlled proliferation during genesis of the cardiac outflow tract and the pituitary gland. As a downstre...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(02)00801-8
更新日期:2002-12-01 00:00:00
abstract::When faced with proteotoxic stress, cells mount adaptive responses to eliminate aberrant proteins. Adaptive responses increase the expression of protein folding and degradation factors to enhance the cellular quality control machinery. However, it is unclear whether and how this augmented machinery acquires new activi...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2018.04.027
更新日期:2018-06-21 00:00:00
abstract::A defining feature of centromeres is the presence of the histone H3 variant CENP-A(Cnp1). It is not known how CENP-A(Cnp1) is specifically delivered to, and assembled into, centromeric chromatin. Through a screen for factors involved in kinetochore integrity in fission yeast, we identified Sim3. Sim3 is homologous to ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.10.010
更新日期:2007-12-28 00:00:00
abstract::Metazoan histone mRNAs are unique: their pre-mRNAs contain no introns, and the mRNAs are not polyadenylated, ending instead in a conserved stem-loop structure. In Drosophila, canonical poly(A) signals are located downstream of the normal cleavage site of each histone gene and are utilized when histone 3' end formation...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2009.04.024
更新日期:2009-05-15 00:00:00
abstract::Streptolydigin (Stl) is a potent inhibitor of bacterial RNA polymerases (RNAPs). The 2.4 A resolution structure of the Thermus thermophilus RNAP-Stl complex showed that, in full agreement with the available genetic data, the inhibitor binding site is located 20 A away from the RNAP active site and encompasses the brid...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2005.07.020
更新日期:2005-09-02 00:00:00
abstract::Bicoid (BCD), the anterior determinant of Drosophila, controls embryonic gene expression by transcriptional activation and translational repression. Both functions require the homeodomain (HD), which recognizes DNA motifs at target gene enhancers and a specific sequence interval in the 3' untranslated region of caudal...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80434-7
更新日期:2000-02-01 00:00:00
abstract::The Bloom syndrome helicase BLM and topoisomerase-IIβ-binding protein 1 (TopBP1) are key regulators of genome stability. It was recently proposed that BLM phosphorylation on Ser338 mediates its interaction with TopBP1, to protect BLM from ubiquitylation and degradation (Wang et al., 2013). Here, we show that the BLM-T...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2015.02.012
更新日期:2015-03-19 00:00:00
abstract::Cells undergo apoptosis upon exposure to severe DNA damage stress. Under this condition, p73 is phosphorylated and activated by c-Abl. The transcription coactivator Yap1 binds p73 to generate a complex that escapes p73 proteasomal degradation and recruits p300 to support transcription of proapoptotic genes. However, t...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.12.022
更新日期:2008-02-15 00:00:00
abstract::Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3, reported here in a binary complex with its immunity protein (IP...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00396-3
更新日期:2001-11-01 00:00:00
abstract::Cardiac disease remains the leading cause of morbidity and mortality worldwide. The β1-adrenergic receptor (β1-AR) is a major regulator of cardiac functions and is downregulated in the majority of heart failure cases. A key physiological process is the activation of heterotrimeric G-protein Gs by β1-ARs, leading to in...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2020.08.001
更新日期:2020-10-01 00:00:00
abstract::The crystal structure of the autoinhibited form of Hck has been determined at 2.0 A resolution, in complex with a specific pyrazolo pyrimidine-type inhibitor, PP1. The activation segment, a key regulatory component of the catalytic domain, is unphosphorylated and is visualized in its entirety. Tyr-416, the site of act...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80357-3
更新日期:1999-05-01 00:00:00
abstract::MeCP2 is an essential transcriptional repressor that mediates gene silencing through binding to methylated DNA. Binding specificity has been thought to depend on hydrophobic interactions between cytosine methyl groups and a hydrophobic patch within the methyl-CpG-binding domain (MBD). X-ray analysis of a methylated DN...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2007.12.028
更新日期:2008-02-29 00:00:00
abstract::Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engageme...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2017.06.010
更新日期:2017-07-20 00:00:00
abstract::Heparan sulfate proteoglycans (HSPGs) influence the signaling of many growth factors, and recent work shows that they can promote VEGF signaling in trans from the surface of an adjacent cell. This mode of signaling also alters the activation state and internalization of VEGF receptors. ...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2006.05.004
更新日期:2006-05-19 00:00:00
abstract::ClpXP is a protein machine composed of the ClpX ATPase, a member of the Clp/Hsp100 family of remodeling enzymes, and the ClpP peptidase. Here, ClpX and ClpXP are shown to catalyze denaturation of GFP modified with an ssrA degradation tag. ClpX translocates this denatured protein into the proteolytic chamber of ClpP an...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80243-9
更新日期:2000-04-01 00:00:00
abstract::Tethering of synaptic vesicles (SVs) to the active zone determines synaptic strength, although the molecular basis governing SV tethering is elusive. Here, we discover that small unilamellar vesicles (SUVs) and SVs from rat brains coat on the surface of condensed liquid droplets formed by active zone proteins RIM, RIM...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2020.10.029
更新日期:2021-01-07 00:00:00
abstract::Mammalian cells constantly monitor and respond to a myriad of extracellular signals, often by using cell surface receptors. Two important classes of cell surface receptors include the receptor tyrosine kinases, which recognize peptide growth factors such as insulin, and the integrins, which most often mediate binding ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80004-0
更新日期:1997-12-01 00:00:00
abstract::The stress response in eukaryotic cells often inhibits translation initiation and leads to the formation of cytoplasmic RNA-protein complexes referred to as stress granules. Stress granules contain nontranslating mRNAs, translation initiation components, and many additional proteins affecting mRNA function. Stress gra...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2009.11.020
更新日期:2009-12-25 00:00:00