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Crystal structure of colicin E3: implications for cell entry and ribosome inactivation.

Abstract：

:Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3, reported here in a binary complex with its immunity protein (IP), reveals a Y-shaped molecule with the receptor binding domain forming a 100 A long stalk and the two globular heads of the translocation domain (T) and the catalytic domain (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active site, IP prevents access of the active site to the ribosome.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Soelaiman S,Jakes K,Wu N,Li C,Shoham M

doi

10.1016/s1097-2765(01)00396-3

subject

Has Abstract

pub_date

2001-11-01 00:00:00

pages

1053-62

issue

5

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(01)00396-3

journal_volume

8

pub_type

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