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Identification of bacteriophage-encoded anti-sRNAs in pathogenic Escherichia coli.

Abstract：

:In bacteria, Hfq is a core RNA chaperone that catalyzes the interaction of mRNAs with regulatory small RNAs (sRNAs). To determine in vivo RNA sequence requirements for Hfq interactions, and to study riboregulation in a bacterial pathogen, Hfq was UV crosslinked to RNAs in enterohemorrhagic Escherichia coli (EHEC). Hfq bound repeated trinucleotide motifs of A-R-N (A-A/G-any nucleotide) often associated with the Shine-Dalgarno translation initiation sequence in mRNAs. These motifs overlapped or were adjacent to the mRNA sequences bound by sRNAs. In consequence, sRNA-mRNA duplex formation will displace Hfq, promoting recycling. Fifty-five sRNAs were identified within bacteriophage-derived regions of the EHEC genome, including some of the most abundant Hfq-interacting sRNAs. One of these (AgvB) antagonized the function of the core genome regulatory sRNA, GcvB, by mimicking its mRNA substrate sequence. This bacteriophage-encoded "anti-sRNA" provided EHEC with a growth advantage specifically in bovine rectal mucus recovered from its primary colonization site in cattle.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Tree JJ,Granneman S,McAteer SP,Tollervey D,Gally DL

doi

10.1016/j.molcel.2014.05.006

subject

Has Abstract

pub_date

2014-07-17 00:00:00

pages

199-213

issue

2

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(14)00400-6

journal_volume

55

pub_type

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