Hematopoietic Signaling Mechanism Revealed from a Stem/Progenitor Cell Cistrome.

abstract：:Heterochromatin is required to restrict aberrant expression of retrotransposons, but it remains poorly defined due to the underlying repeat-rich sequences. We dissected Suv39h-dependent histone H3 lysine 9 trimethylation (H3K9me3) by genome-wide ChIP sequencing in mouse embryonic stem cells (ESCs). Refined bioinformat...

journal_title：Molecular cell

authors： Bulut-Karslioglu A,De La Rosa-Velázquez IA,Ramirez F,Barenboim M,Onishi-Seebacher M,Arand J,Galán C,Winter GE,Engist B,Gerle B,O'Sullivan RJ,Martens JH,Walter J,Manke T,Lachner M,Jenuwein T

更新日期：2014-07-17 00:00:00

abstract：:Clathrin-dependent endocytosis has long been presented as the only efficient mechanism by which transmembrane receptors are internalized. We selectively blocked this process using dominant-negative mutants of Eps15 and showed that clathrin-mediated endocytosis of transferrin was inhibited, while endocytosis of interle...

journal_title：Molecular cell

authors： Lamaze C,Dujeancourt A,Baba T,Lo CG,Benmerah A,Dautry-Varsat A

更新日期：2001-03-01 00:00:00

abstract：:Diaphanous-related formins (DRFs) regulate dynamics of unbranched actin filaments during cell contraction and cytokinesis. DRFs are autoinhibited through intramolecular binding of a Diaphanous autoinhibitory domain (DAD) to a conserved N-terminal regulatory element. Autoinhibition is relieved through binding of the GT...

journal_title：Molecular cell

authors： Otomo T,Otomo C,Tomchick DR,Machius M,Rosen MK

更新日期：2005-04-29 00:00:00

abstract：:Both p53 and Mdmx are ubiquitinated and degraded by the same E3 ligase Mdm2; interestingly, however, while p53 is rapidly degraded by Mdm2, Mdmx is a stable protein in most cancer cells. Thus, the mechanism by which Mdmx is degraded by Mdm2 needs further elucidation. Here, we identified the noncoding 5S rRNA as a majo...

journal_title：Molecular cell

authors： Li M,Gu W

更新日期：2011-09-16 00:00:00

abstract：:Small DNA viruses force quiescent cells to reenter the cell cycle in order to replicate their DNA. We report here that the adenovirus E1A protein creates an S phase environment in quiescent cells by overcoming the nucleosomal repression of E2F-targeted genes. These genes are surrounded by Lys-9-methylated H3 histones,...

journal_title：Molecular cell

authors： Ghosh MK,Harter ML

更新日期：2003-07-01 00:00:00

abstract：:mRNAs form ribonucleoprotein complexes (mRNPs) by association with proteins that are crucial for mRNA metabolism. While the mRNP proteome has been well characterized, little is known about mRNP organization. Using a single-molecule approach, we show that mRNA conformation changes depending on its cellular localization...

journal_title：Molecular cell

authors： Adivarahan S,Livingston N,Nicholson B,Rahman S,Wu B,Rissland OS,Zenklusen D

更新日期：2018-11-15 00:00:00

abstract：:The ribosome, a ribonucleoprotein machine for protein synthesis, can also serve as an abundant nutrient source under starvation conditions. In a recent issue of Science, Wyant et al. (2018) discovered a specialized "spoon" to "scoop up" more ribosomes for degradation by autophagy. ...

journal_title：Molecular cell

authors： Nakatogawa H

更新日期：2018-07-19 00:00:00

abstract：:The disulfide relay system in the intermembrane space of mitochondria is of crucial importance for mitochondrial biogenesis. Major players in this pathway are the oxidoreductase Mia40 that oxidizes substrates and the sulfhydryl oxidase Erv1 that reoxidizes Mia40. To analyze in detail the mechanism of this oxidative pa...

journal_title：Molecular cell

authors： Bien M,Longen S,Wagener N,Chwalla I,Herrmann JM,Riemer J

更新日期：2010-02-26 00:00:00

abstract：:One of the earliest steps in metazoan pre-mRNA splicing involves binding of U2 snRNP auxiliary factor (U2AF) 65 KDa subunit to the polypyrimidine (Py) tract and of the 35 KDa subunit to the invariant AG dinucleotide at the intron 3' end. Here we use in vitro and in vivo depletion, as well as reconstitution assays usin...

journal_title：Molecular cell

authors： Tavanez JP,Madl T,Kooshapur H,Sattler M,Valcárcel J

更新日期：2012-02-10 00:00:00

abstract：:Calreticulin and calnexin are homologous lectins that serve as molecular chaperones for glycoproteins in the endoplasmic reticulum of eukaryotic cells. Here we show that calreticulin depletion specifically accelerates the maturation of cellular and viral glycoproteins with a modest decrease in folding efficiency. Caln...

journal_title：Molecular cell

authors： Molinari M,Eriksson KK,Calanca V,Galli C,Cresswell P,Michalak M,Helenius A

更新日期：2004-01-16 00:00:00

abstract：:BIK protein is an initiator of mitochondrial apoptosis, and BIK expression is induced by proapoptotic signals, including DNA damage. Here, we demonstrate that 3' end processing and expression of BIK mRNA are controlled by the nuclear PI4,5P(2)-regulated poly(A) polymerase Star-PAP downstream of DNA damage. Nuclear PKC...

journal_title：Molecular cell

authors： Li W,Laishram RS,Ji Z,Barlow CA,Tian B,Anderson RA

更新日期：2012-01-13 00:00:00

abstract：:Metazoan histone mRNAs are unique: their pre-mRNAs contain no introns, and the mRNAs are not polyadenylated, ending instead in a conserved stem-loop structure. In Drosophila, canonical poly(A) signals are located downstream of the normal cleavage site of each histone gene and are utilized when histone 3' end formation...

journal_title：Molecular cell

authors： Sullivan KD,Steiniger M,Marzluff WF

更新日期：2009-05-15 00:00:00

abstract：:The steroid hormone ecdysone signals the stage-specific programmed cell death of the larval salivary glands during Drosophila metamorphosis. This response is preceded by an ecdysone-triggered switch in gene expression in which the diap2 death inhibitor is repressed and the reaper (rpr) and head involution defective (h...

journal_title：Molecular cell

authors： Jiang C,Lamblin AF,Steller H,Thummel CS

更新日期：2000-03-01 00:00:00

abstract：:Zinc is an essential trace element, and impaired zinc homeostasis is implicated in the pathogenesis of various human diseases. However, the mechanisms cells use to respond to zinc deficiency are poorly understood. We previously reported that amyotrophic lateral sclerosis (ALS)-linked pathogenic mutants of SOD1 cause c...

journal_title：Molecular cell

authors： Homma K,Fujisawa T,Tsuburaya N,Yamaguchi N,Kadowaki H,Takeda K,Nishitoh H,Matsuzawa A,Naguro I,Ichijo H

更新日期：2013-10-10 00:00:00

abstract：:The Y family of DNA polymerases plays crucial roles in carrying out translesion synthesis past damaged bases in DNA. Several recent papers suggest that they might have other roles as well in gene conversion, in nucleotide excision repair (NER), and in DNA replication under stressed conditions. ...

journal_title：Molecular cell

authors： Lehmann AR

更新日期：2006-11-17 00:00:00

abstract：:Telomeres protect chromosome ends from fusing to double-stranded breaks (DSBs). Using a quantitative real-time PCR assay, we show that nonhomologous end joining between a telomere and an inducible DSB was undetectable in wild-type cells, but occurred within a few hours of DSB induction in approximately 1/2000 genomes ...

journal_title：Molecular cell

authors： Chan SW,Blackburn EH

更新日期：2003-05-01 00:00:00

abstract：:Polymerase gamma, which replicates and repairs mitochondrial DNA, requires the Pol gamma B subunit for processivity. We determined the crystal structure of mouse Pol gamma B, a core component of the mitochondrial replication machinery. Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through a...

journal_title：Molecular cell

authors： Carrodeguas JA,Theis K,Bogenhagen DF,Kisker C

更新日期：2001-01-01 00:00:00

abstract：:In this issue of Molecular Cell, Guo et al. (2014) report that misfolded or aggregated nuclear proteins, such as pathogenic polyQ proteins, are cleared by a SUMO-dependent quality control pathway, which involves the E3 SUMO ligase PML and the SUMO-targeted ubiquitin ligase RNF4. ...

journal_title：Molecular cell

authors： Gärtner A,Muller S

更新日期：2014-07-03 00:00:00

abstract：:When inappropriate DNA structures arise, they are sensed by DNA structure-dependent checkpoint pathways and subsequently repaired. Recruitment of checkpoint proteins to such structures precedes recruitment of proteins involved in DNA metabolism. Thus, checkpoints can regulate DNA metabolism. We show that fission yeast...

journal_title：Molecular cell

authors： Furuya K,Miyabe I,Tsutsui Y,Paderi F,Kakusho N,Masai H,Niki H,Carr AM

更新日期：2010-11-24 00:00:00

abstract：:SOCS1 is lost in many human tumors, but its tumor suppression activities are not well understood. We report that SOCS1 is required for transcriptional activity, DNA binding, and serine 15 phosphorylation of p53 in the context of STAT5 signaling. In agreement, inactivation of SOCS1 disabled p53-dependent senescence in ...

journal_title：Molecular cell

authors： Calabrese V,Mallette FA,Deschênes-Simard X,Ramanathan S,Gagnon J,Moores A,Ilangumaran S,Ferbeyre G

更新日期：2009-12-11 00:00:00

abstract：:In budding yeast, the nuclear RNA surveillance system is active on all pre-mRNA transcripts and modulated by nutrient availability. To test the role of nuclear surveillance in reprogramming gene expression, we identified transcriptome-wide binding sites for RNA polymerase II and the exosome cofactors Mtr4 (TRAMP compl...

journal_title：Molecular cell

authors： Bresson S,Tuck A,Staneva D,Tollervey D

更新日期：2017-03-02 00:00:00

abstract：:The protein kinase B-RAF is mutated in approximately 7% of human cancers. Most mutations are activating, but, surprisingly, a small number have reduced kinase activity. However, the latter can still stimulate cellular signaling through the MEK-ERK pathway because they activate the related family member C-RAF. We exami...

journal_title：Molecular cell

authors： Garnett MJ,Rana S,Paterson H,Barford D,Marais R

更新日期：2005-12-22 00:00:00

abstract：:Upon recruitment to active enhancers and promoters, RNA polymerase II (Pol II) generates short non-coding transcripts of unclear function. The mechanisms that control the length and the amount of ncRNAs generated by cis-regulatory elements are largely unknown. Here, we show that the adaptor protein WDR82 and its assoc...

journal_title：Molecular cell

authors： Austenaa LM,Barozzi I,Simonatto M,Masella S,Della Chiara G,Ghisletti S,Curina A,de Wit E,Bouwman BA,de Pretis S,Piccolo V,Termanini A,Prosperini E,Pelizzola M,de Laat W,Natoli G

更新日期：2015-11-05 00:00:00

abstract：:The transition from transcription initiation to elongation at promoters of primary response genes (PRGs) in metazoan cells is controlled by inducible transcription factors, which utilize P-TEFb to phosphorylate RNA polymerase II (Pol II) in response to stimuli. Prior to stimulation, a fraction of P-TEFb is recruited t...

journal_title：Molecular cell

authors： McNamara RP,Reeder JE,McMillan EA,Bacon CW,McCann JL,D'Orso I

更新日期：2016-01-07 00:00:00

abstract：:In this issue of Molecular Cell, Rinaldi et al. (2021) reveal how nutrient availability in the metastatic niche of breast cancer cells shapes their growth signaling, highlighting potential avenues for therapeutic interventions. ...

journal_title：Molecular cell

authors： Audet-Delage Y,St-Pierre J

更新日期：2021-01-21 00:00:00

abstract：:Circadian rhythms in mammals are generated by a transcriptional negative feedback loop that is driven primarily by oscillations of PER and CRY, which inhibit their own transcriptional activators, CLOCK and BMAL1. Current models posit that CRY is the dominant repressor, while PER may play an accessory role. In this stu...

journal_title：Molecular cell

authors： Chen R,Schirmer A,Lee Y,Lee H,Kumar V,Yoo SH,Takahashi JS,Lee C

更新日期：2009-11-13 00:00:00

abstract：:HIV-1 Vpu interacts with CD4 in the endoplasmic reticulum and triggers CD4 degradation, presumably by proteasomes. Human beta TrCP identified by interaction with Vpu connects CD4 to this proteolytic machinery, and CD4-Vpu-beta TrCP ternary complexes have been detected by coimmunoprecipitation. beta TrCP binding to Vpu...

journal_title：Molecular cell

authors： Margottin F,Bour SP,Durand H,Selig L,Benichou S,Richard V,Thomas D,Strebel K,Benarous R

更新日期：1998-03-01 00:00:00

abstract：:The Ska complex is an essential mitotic component required for accurate cell division in human cells. It is composed of three subunits that function together to establish stable kinetochore-microtubule interactions in concert with the Ndc80 network. We show that the structure of the Ska core complex is a W-shaped dime...

journal_title：Molecular cell

authors： Jeyaprakash AA,Santamaria A,Jayachandran U,Chan YW,Benda C,Nigg EA,Conti E

更新日期：2012-05-11 00:00:00

abstract：:Mitochondrial crista structure partitions vital cellular reactions and is precisely regulated by diverse cellular signals. Here, we show that, in Drosophila, mitochondrial cristae undergo dynamic remodeling among distinct subcellular regions and the Parkinson's disease (PD)-linked Ser/Thr kinase PINK1 participates in ...

journal_title：Molecular cell

authors： Tsai PI,Lin CH,Hsieh CH,Papakyrikos AM,Kim MJ,Napolioni V,Schoor C,Couthouis J,Wu RM,Wszolek ZK,Winter D,Greicius MD,Ross OA,Wang X

更新日期：2018-03-01 00:00:00

abstract：:Study of mutant phenotypes is a fundamental method for understanding gene function. The construction of a near-complete collection of yeast knockouts (YKO) and the unique molecular barcodes (or TAGs) that identify each strain has enabled quantitative functional profiling of Saccharomyces cerevisiae. By using these TAG...

journal_title：Molecular cell

authors： Pan X,Yuan DS,Xiang D,Wang X,Sookhai-Mahadeo S,Bader JS,Hieter P,Spencer F,Boeke JD

更新日期：2004-11-05 00:00:00