Interleukin 2 receptors and detergent-resistant membrane domains define a clathrin-independent endocytic pathway.

abstract：:MicroRNAs (miRNAs) inhibit mRNA expression in general by base pairing to the 3'UTR of target mRNAs and consequently inhibiting translation and/or initiating poly(A) tail deadenylation and mRNA destabilization. Here we examine the mechanism and kinetics of miRNA-mediated deadenylation in mouse Krebs-2 ascites extract. ...

journal_title：Molecular cell

authors： Fabian MR,Mathonnet G,Sundermeier T,Mathys H,Zipprich JT,Svitkin YV,Rivas F,Jinek M,Wohlschlegel J,Doudna JA,Chen CY,Shyu AB,Yates JR 3rd,Hannon GJ,Filipowicz W,Duchaine TF,Sonenberg N

更新日期：2009-09-24 00:00:00

abstract：:Gene-targeted knockout mice have been generated lacking the major uracil-DNA glycosylase, UNG. In contrast to ung- mutants of bacteria and yeast, such mice do not exhibit a greatly increased spontaneous mutation frequency. However, there is only slow removal of uracil from misincorporated dUMP in isolated ung-/- nucle...

journal_title：Molecular cell

authors： Nilsen H,Rosewell I,Robins P,Skjelbred CF,Andersen S,Slupphaug G,Daly G,Krokan HE,Lindahl T,Barnes DE

更新日期：2000-06-01 00:00:00

abstract：:The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II) comprises multiple heptapeptide repeats of the consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Reversible phosphorylation of Ser2, Ser5, and Ser7 during the transcription cycle mediates the sequential recruitment of transcription/RNA pr...

journal_title：Molecular cell

authors： Egloff S,Zaborowska J,Laitem C,Kiss T,Murphy S

更新日期：2012-01-13 00:00:00

abstract：:Mutation and prevalence of pathogenic viruses prompt the development of broad-spectrum antiviral strategies. Viperin is a potent antiviral protein that inhibits a broad range of viruses. Unexpectedly, we found that Viperin protein production in epithelium is defective in response to both viruses and interferons (IFNs)...

journal_title：Molecular cell

authors： Yuan Y,Miao Y,Qian L,Zhang Y,Liu C,Liu J,Zuo Y,Feng Q,Guo T,Zhang L,Chen X,Jin L,Huang F,Zhang H,Zhang W,Li W,Xu G,Zheng H

更新日期：2020-02-20 00:00:00

abstract：:Homologous recombination is essential for preserving genome integrity. Joining of homologous DNA molecules through strand exchange, a pivotal step in recombination, is mediated by RAD51. Here, we identify RAD51AP1 as a RAD51 accessory protein that specifically stimulates joint molecule formation through the combinatio...

journal_title：Molecular cell

authors： Modesti M,Budzowska M,Baldeyron C,Demmers JA,Ghirlando R,Kanaar R

更新日期：2007-11-09 00:00:00

abstract：:Nucleosomes are surprisingly dynamic structures in vivo, showing transcription-independent exchange of histones H2A-H2B genome-wide and exchange of H3-H4 mainly within the promoters of transcribed genes. In addition, nucleosomes are disrupted in front of and reassembled behind the elongating RNA polymerase. Here we sh...

journal_title：Molecular cell

authors： Jamai A,Puglisi A,Strubin M

更新日期：2009-08-14 00:00:00

abstract：:Actin-related proteins (Arps) and conventional actin are enigmatic components of many chromatin-remodeling enzyme complexes. The yeast INO80 ATP-dependent chromatin-remodeling complex contains stoichiometric amounts of Arp4, Arp5, Arp8, and actin. Here we have revealed functions of Arp5 and Arp8 by analysis of mutants...

journal_title：Molecular cell

authors： Shen X,Ranallo R,Choi E,Wu C

更新日期：2003-07-01 00:00:00

abstract：:An exciting study in this issue reveals how binding of the microtubule associated protein TPX2 to the mitotic kinase Aurora A induces a conformational change. This moves the phosphorylated activation domain into a more compact position within the kinase core, providing a better substrate binding platform and hiding th...

journal_title：Molecular cell

authors： Glover DM

更新日期：2003-10-01 00:00:00

abstract：:HAT1 is an essential gene in mammals that acetylates newly synthesized histone H4 and is involved in chromatin assembly and genome maintenance. In this issue of Molecular Cell, Gruber et al. (2019) show that HAT1 binds to and stimulates H4 gene expression in a growth-factor- and glucose-dependent manner. ...

journal_title：Molecular cell

authors： Denu JM

更新日期：2019-08-22 00:00:00

abstract：:Dbh is a Y family translesion DNA polymerase that accurately bypasses some damaged forms of deoxyguanosine, but also generates single-base deletion errors at frequencies of up to 50%, in specific hot spot sequences. We describe preinsertion binary, insertion ternary, and postinsertion binary crystal structures of Dbh ...

journal_title：Molecular cell

authors： Wilson RC,Pata JD

更新日期：2008-03-28 00:00:00

abstract：:The bHLH factors HAND1 and HAND2 are required for heart, vascular, neuronal, limb, and extraembryonic development. Unlike most bHLH proteins, HAND factors exhibit promiscuous dimerization properties. We report that phosphorylation/dephosphorylation via PKA, PKC, and a specific heterotrimeric protein phosphatase 2A (PP...

journal_title：Molecular cell

authors： Firulli BA,Howard MJ,McDaid JR,McIlreavey L,Dionne KM,Centonze VE,Cserjesi P,Virshup DM,Firulli AB

更新日期：2003-11-01 00:00:00

abstract：:tRNAs reading four-codon families often have a modified uridine, cmo(5)U(34), at the wobble position of the anticodon. Here, we examine the effects on the decoding mechanism of a cmo(5)U modification in tRNA(1B)(Ala), anticodon C(36)G(35)cmo(5)U(34). tRNA(1B)(Ala) reads its cognate codons in a manner that is very simi...

journal_title：Molecular cell

authors： Kothe U,Rodnina MV

更新日期：2007-01-12 00:00:00

abstract：:The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and GSK3beta, forms a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of beta-catenin by the proteasome. Siah-1, the human homolog of Drosophila seven in absentia, is a p53-inducible mediator of cell c...

journal_title：Molecular cell

authors： Liu J,Stevens J,Rote CA,Yost HJ,Hu Y,Neufeld KL,White RL,Matsunami N

更新日期：2001-05-01 00:00:00

abstract：:Transcript-selective translational regulation of epithelial-mesenchymal transition (EMT) by transforming growth factor-β (TGF-β) is directed by the hnRNP E1-containing TGF-β-activated-translational (BAT) mRNP complex. Herein, eukaryotic elongation factor-1 A1 (eEF1A1) is identified as an integral component of the BAT ...

journal_title：Molecular cell

authors： Hussey GS,Chaudhury A,Dawson AE,Lindner DJ,Knudsen CR,Wilce MC,Merrick WC,Howe PH

更新日期：2011-02-18 00:00:00

abstract：:We used computational algorithms to find conserved sequences in the 3' untranslated region (UTR) of transcripts that exhibited rapid decay in primary human T cells and found that the consensus sequence UGUUUGUUUGU, which we have termed a GU-rich element (GRE), was enriched in short-lived transcripts. Using a tet-off r...

journal_title：Molecular cell

authors： Vlasova IA,Tahoe NM,Fan D,Larsson O,Rattenbacher B,Sternjohn JR,Vasdewani J,Karypis G,Reilly CS,Bitterman PB,Bohjanen PR

更新日期：2008-02-01 00:00:00

abstract：:While much research has examined the use of glucose and glutamine by tumor cells, many cancers instead prefer to metabolize fats. Despite the pervasiveness of this phenotype, knowledge of pathways that drive fatty acid oxidation (FAO) in cancer is limited. Prolyl hydroxylase domain proteins hydroxylate substrate proli...

journal_title：Molecular cell

authors： German NJ,Yoon H,Yusuf RZ,Murphy JP,Finley LW,Laurent G,Haas W,Satterstrom FK,Guarnerio J,Zaganjor E,Santos D,Pandolfi PP,Beck AH,Gygi SP,Scadden DT,Kaelin WG Jr,Haigis MC

更新日期：2016-09-15 00:00:00

abstract：:Programmed cell death is a gene-directed process involved in the development and homeostasis of multicellular organisms. The most common mode of programmed cell death is apoptosis, which is characterized by a stereotypical set of biochemical and morphological hallmarks. Here we report that Escherichia coli also exhibi...

journal_title：Molecular cell

authors： Dwyer DJ,Camacho DM,Kohanski MA,Callura JM,Collins JJ

更新日期：2012-06-08 00:00:00

abstract：:Prions constitute a rare class of protein, which can switch to a robust amyloid form and then propagate that form in the absence of a nucleic acid determinant, thereby creating a unique, protein-only infectious agent. Details of the mechanism that drives conversion to the prion form and then subsequent propagation of ...

journal_title：Molecular cell

authors： Tuite MF,Koloteva-Levin N

更新日期：2004-06-04 00:00:00

abstract：:Oocyte quality control culls eggs with defects in meiosis. In mouse, oocyte death can be triggered by defects in chromosome synapsis and recombination, which involve repair of DNA double-strand breaks (DSBs) between homologous chromosomes. We show that RNF212, a SUMO ligase required for crossing over, also mediates oo...

journal_title：Molecular cell

authors： Qiao H,Rao HBDP,Yun Y,Sandhu S,Fong JH,Sapre M,Nguyen M,Tham A,Van BW,Chng TYH,Lee A,Hunter N

更新日期：2018-10-18 00:00:00

abstract：:DNA replication introduces a dosage imbalance between early and late replicating genes. In budding yeast, buffering gene expression against this imbalance depends on marking replicated DNA by H3K56 acetylation (H3K56ac). Whether additional processes are required for suppressing transcription from H3K56ac-labeled DNA r...

journal_title：Molecular cell

authors： Voichek Y,Mittelman K,Gordon Y,Bar-Ziv R,Lifshitz Smit D,Shenhav R,Barkai N

更新日期：2018-06-21 00:00:00

abstract：:DNA methylation directed by 24-nucleotide (nt) small interfering RNAs (siRNAs) plays critical roles in gene regulation and transposon silencing in Arabidopsis. 24-nt siRNAs are known to be processed from double-stranded RNAs by Dicer-like 3 (DCL3) and loaded into the effector Argonaute 4 (AGO4). Here we report a disti...

journal_title：Molecular cell

authors： Ye R,Chen Z,Lian B,Rowley MJ,Xia N,Chai J,Li Y,He XJ,Wierzbicki AT,Qi Y

更新日期：2016-01-21 00:00:00

abstract：:Autophagy constitutes a prominent mechanism through which eukaryotic cells preserve homeostasis in baseline conditions and in response to perturbations of the intracellular or extracellular microenvironment. Autophagic responses can be relatively non-selective or target a specific subcellular compartment. At least in ...

journal_title：Molecular cell

authors： Sica V,Galluzzi L,Bravo-San Pedro JM,Izzo V,Maiuri MC,Kroemer G

更新日期：2015-08-20 00:00:00

abstract：:The protein 53BP1 plays a central regulatory role in DNA double-strand break repair. 53BP1 relocates to chromatin by recognizing RNF168-mediated mono-ubiquitylation of histone H2A Lys15 in the nucleosome core particle dimethylated at histone H4 Lys20 (NCP-ubme). 53BP1 relocation is terminated by ubiquitin ligases RNF1...

journal_title：Molecular cell

authors： Hu Q,Botuyan MV,Cui G,Zhao D,Mer G

更新日期：2017-05-18 00:00:00

abstract：:By using single-molecule fluorescence resonance energy transfer (smFRET), we observe the real-time dynamic coupling between the ribosome, labeled at the L1 stalk, and transfer RNA (tRNA). We find that an interaction between the ribosomal L1 stalk and the newly deacylated tRNA is established spontaneously upon peptide ...

journal_title：Molecular cell

authors： Fei J,Kosuri P,MacDougall DD,Gonzalez RL Jr

更新日期：2008-05-09 00:00:00

abstract：:Reactive oxygen species (ROS)-induced activation of Apoptosis signal-regulating kinase 1 (ASK1) plays crucial roles in oxidative stress-mediated cell death through the activation of the JNK and p38 MAPK pathways. However, the regulatory mechanism of ASK1 in the oxidative stress response remains to be elucidated. Here,...

journal_title：Molecular cell

authors： Sekine Y,Hatanaka R,Watanabe T,Sono N,Iemura S,Natsume T,Kuranaga E,Miura M,Takeda K,Ichijo H

更新日期：2012-12-14 00:00:00

abstract：:Thousands of cis-elements in genomes are predicted to have vital functions. Although conservation, activity in surrogate assays, polymorphisms, and disease mutations provide functional clues, deletion from endogenous loci constitutes the gold-standard test. A GATA-2-binding, Gata2 intronic cis-element (+9.5) required ...

journal_title：Molecular cell

authors： Hewitt KJ,Kim DH,Devadas P,Prathibha R,Zuo C,Sanalkumar R,Johnson KD,Kang YA,Kim JS,Dewey CN,Keles S,Bresnick EH

更新日期：2015-07-02 00:00:00

abstract：:The 3D structures of all 12 mispairs formed in the active site of a DNA polymerase help explain their differential effects on polymerase stalling and on translocation of the primer terminus to the enzyme's proofreading site. ...

journal_title：Molecular cell

authors： Jiricny J

更新日期：2004-03-26 00:00:00

abstract：:DNA lesions caused by UV damage are thought to be repaired solely by the nucleotide excision repair (NER) pathway in human cells. Patients carrying mutations within genes functioning in this pathway display a range of pathologies, including an increased susceptibility to cancer, premature aging, and neurological defec...

journal_title：Molecular cell

authors： Mazouzi A,Battistini F,Moser SC,Ferreira da Silva J,Wiedner M,Owusu M,Lardeau CH,Ringler A,Weil B,Neesen J,Orozco M,Kubicek S,Loizou JI

更新日期：2017-11-16 00:00:00

abstract：:Cardozo Gizzi et al. (2019) develop a new sequential imaging methodology (Hi-M) for observing chromosome structure in the Drosophila blastoderm and find that topological domains in single nuclei change in response to transcriptional activation. ...

journal_title：Molecular cell

authors： Larson DR

更新日期：2019-04-04 00:00:00

abstract：:Sphingosine 1-phosphate (S1P) functions not only as a bioactive lipid molecule, but also as an important intermediate of the sole sphingolipid-to-glycerolipid metabolic pathway. However, the precise reactions and the enzymes involved in this pathway remain unresolved. We report here that yeast HFD1 and the Sjögren-Lar...

journal_title：Molecular cell

authors： Nakahara K,Ohkuni A,Kitamura T,Abe K,Naganuma T,Ohno Y,Zoeller RA,Kihara A

更新日期：2012-05-25 00:00:00