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Structural basis of Rho GTPase-mediated activation of the formin mDia1.

Abstract：

:Diaphanous-related formins (DRFs) regulate dynamics of unbranched actin filaments during cell contraction and cytokinesis. DRFs are autoinhibited through intramolecular binding of a Diaphanous autoinhibitory domain (DAD) to a conserved N-terminal regulatory element. Autoinhibition is relieved through binding of the GTPase RhoA to the N-terminal element. We report the crystal structure of the dimeric regulatory domain of the DRF, mDia1. Dimerization is mediated by an intertwined six-helix bundle, from which extend two Diaphanous inhibitory domains (DIDs) composed of five armadillo repeats. NMR and biochemical mapping indicate the RhoA and DAD binding sites on the DID partially overlap, explaining activation of mDia1 by the GTPase. RhoA binding also requires an additional structurally independent segment adjacent to the DID. This regulatory construction, involving a GTPase binding site spanning a flexibly tethered arm and the inhibitory module, is observed in many autoinhibited effectors of Ras superfamily GTPases, suggesting evolutionary pressure for this design.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Otomo T,Otomo C,Tomchick DR,Machius M,Rosen MK

doi

10.1016/j.molcel.2005.04.002

subject

Has Abstract

pub_date

2005-04-29 00:00:00

pages

273-81

issue

3

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(05)01226-8

journal_volume

18

pub_type

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