Bax exists in a dynamic equilibrium between the cytosol and mitochondria to control apoptotic priming.

abstract：:Faithful propagation of functionally distinct chromatin states is crucial for maintaining cellular identity, and its breakdown can lead to diseases such as cancer. Whereas mechanisms that sustain repressed states have been intensely studied, regulatory circuits that protect active chromatin from inactivating signals a...

journal_title：Molecular cell

authors： Flury V,Georgescu PR,Iesmantavicius V,Shimada Y,Kuzdere T,Braun S,Bühler M

更新日期：2017-07-20 00:00:00

abstract：:Metazoan histone mRNAs end in a highly conserved stem-loop structure followed by ACCCA. Previous studies have suggested that the stem-loop binding protein (SLBP) is the only protein binding this region. Using RNA affinity purification, we identified a second protein, designated 3'hExo, that contains a SAP and a 3' exo...

journal_title：Molecular cell

authors： Dominski Z,Yang XC,Kaygun H,Dadlez M,Marzluff WF

更新日期：2003-08-01 00:00:00

abstract：:PTPA, an essential and specific activator of protein phosphatase 2A (PP2A), functions as a peptidyl prolyl isomerase (PPIase). We present here the crystal structures of human PTPA and of the two yeast orthologs (Ypa1 and Ypa2), revealing an all alpha-helical protein fold that is radically different from other PPIases....

journal_title：Molecular cell

authors： Leulliot N,Vicentini G,Jordens J,Quevillon-Cheruel S,Schiltz M,Barford D,van Tilbeurgh H,Goris J

更新日期：2006-08-04 00:00:00

abstract：:Eukaryotic gene expression regulation involves thousands of distal regulatory elements. Understanding the quantitative contribution of individual enhancers to gene expression is critical for assessing the role of disease-associated genetic risk variants. Yet, we lack the ability to accurately link genes with their dis...

journal_title：Molecular cell

authors： Vangala P,Murphy R,Quinodoz SA,Gellatly K,McDonel P,Guttman M,Garber M

更新日期：2020-10-15 00:00:00

abstract：:Signal-transduction cascades are usually studied on cell averages, masking variability between individual cells. To address this, we studied in individual cells the dynamic response of ERK2, a well-characterized MAPK signaling protein, which enters the nucleus upon stimulation. Using fluorescent tagging at the endogen...

journal_title：Molecular cell

authors： Cohen-Saidon C,Cohen AA,Sigal A,Liron Y,Alon U

更新日期：2009-12-11 00:00:00

abstract：:FUS is a nuclear RNA-binding protein, and its cytoplasmic aggregation is a pathogenic signature of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). It remains unknown how the FUS-RNA interactions contribute to phase separation and whether its phase behavior is affected by ALS-linked mutations. He...

journal_title：Molecular cell

authors： Niaki AG,Sarkar J,Cai X,Rhine K,Vidaurre V,Guy B,Hurst M,Lee JC,Koh HR,Guo L,Fare CM,Shorter J,Myong S

更新日期：2020-01-02 00:00:00

abstract：:The ER-mitochondrial junction provides a local calcium signaling domain that is critical for both matching energy production with demand and the control of apoptosis. Here, we visualize ER-mitochondrial contact sites and monitor the localized [Ca(2+)] changes ([Ca(2+)](ER-mt)) using drug-inducible fluorescent interorg...

journal_title：Molecular cell

authors： Csordás G,Várnai P,Golenár T,Roy S,Purkins G,Schneider TG,Balla T,Hajnóczky G

更新日期：2010-07-09 00:00:00

abstract：:The structural diversity of glycans on cells-the glycome-is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates. We used maps of g...

journal_title：Molecular cell

authors： Narimatsu Y,Joshi HJ,Nason R,Van Coillie J,Karlsson R,Sun L,Ye Z,Chen YH,Schjoldager KT,Steentoft C,Furukawa S,Bensing BA,Sullam PM,Thompson AJ,Paulson JC,Büll C,Adema GJ,Mandel U,Hansen L,Bennett EP,Varki A,Vak

更新日期：2019-07-25 00:00:00

abstract：:MRE11 within the MRE11-RAD50-NBS1 (MRN) complex acts in DNA double-strand break repair (DSBR), detection, and signaling; yet, how its endo- and exonuclease activities regulate DSBR by nonhomologous end-joining (NHEJ) versus homologous recombination (HR) remains enigmatic. Here, we employed structure-based design with ...

journal_title：Molecular cell

authors： Shibata A,Moiani D,Arvai AS,Perry J,Harding SM,Genois MM,Maity R,van Rossum-Fikkert S,Kertokalio A,Romoli F,Ismail A,Ismalaj E,Petricci E,Neale MJ,Bristow RG,Masson JY,Wyman C,Jeggo PA,Tainer JA

更新日期：2014-01-09 00:00:00

abstract：:Serine/arginine (SR)-rich splicing factors contain an RNA binding domain and an arginine/serine (RS)-rich domain required for protein-protein interactions. In addition to their roles in the basic splicing reaction, SR proteins function as components of splicing enhancer complexes. Here, we investigate the role of RS d...

journal_title：Molecular cell

authors： Graveley BR,Maniatis T

更新日期：1998-04-01 00:00:00

abstract：:In this study, we used Saccharomyces cerevisiae to identify a biological network that prevents the deleterious effects of endogenous reactive oxygen species. The absence of Tsa1, a key peroxiredoxin, caused increased rates of mutations, chromosomal rearrangements, and recombination. Defects in recombinational DNA doub...

journal_title：Molecular cell

authors： Huang ME,Kolodner RD

更新日期：2005-03-04 00:00:00

abstract：:Many DNA viruses that are latent in dividing cells are noncovalent passengers on mitotic chromosomes and require specific viral-encoded and cellular factors for this activity. The chromosomal protein Brd4 is implicated in the hitchhiking of bovine papillomavirus-1 (BPV-1), and the viral protein E2 binds to both plasmi...

journal_title：Molecular cell

authors： Abbate EA,Voitenleitner C,Botchan MR

更新日期：2006-12-28 00:00:00

abstract：:The sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner. The PCNA heterotrimer, but not individual subunits, stimulates the activities of the DNA polymerase, DNA ligase I, and the flap endonuclease (FEN1) of S...

journal_title：Molecular cell

authors： Dionne I,Nookala RK,Jackson SP,Doherty AJ,Bell SD

更新日期：2003-01-01 00:00:00

abstract：:Faithful transmission of genomic information requires tight spatiotemporal regulation of DNA replication factors. In the licensing step of DNA replication, CDT-1 is loaded onto chromatin to subsequently promote the recruitment of additional replication factors, including CDC-45 and GINS. During the elongation step, th...

journal_title：Molecular cell

authors： Franz A,Orth M,Pirson PA,Sonneville R,Blow JJ,Gartner A,Stemmann O,Hoppe T

更新日期：2011-10-07 00:00:00

abstract：:The early detection by the host of invading microorganisms, including viruses, depends on a limited number of specific receptors that recognize pathogen-associated molecular patterns (PAMPs). A few of these PAMPs, including ssRNA and dsRNA, are recognized by Toll-like receptors (TLR)-7/8 and TLR3, respectively. Activa...

journal_title：Molecular cell

authors： Meylan E,Tschopp J

更新日期：2006-06-09 00:00:00

abstract：:In yeast, histone H3/H4 exchange independent of replication is poorly understood. Here, we analyzed the deposition of histone H3 molecules, synthesized during G1, using a high-density microarray histone exchange assay. While we found that H3 exchange in coding regions requires high levels of transcription, promoters e...

journal_title：Molecular cell

authors： Rufiange A,Jacques PE,Bhat W,Robert F,Nourani A

更新日期：2007-08-03 00:00:00

abstract：:Tet(O) belongs to a class of ribosomal protection proteins that mediate tetracycline resistance. It is a G protein that shows significant sequence similarity to elongation factor EF-G. Here we present a cryo-electron microscopic reconstruction, at 16 A resolution, of its complex with the E. coli 70S ribosome. Tet(O) w...

journal_title：Molecular cell

authors： Spahn CM,Blaha G,Agrawal RK,Penczek P,Grassucci RA,Trieber CA,Connell SR,Taylor DE,Nierhaus KH,Frank J

更新日期：2001-05-01 00:00:00

abstract：:FMOC-L-Leucine (F-L-Leu) is a chemically distinct PPARgamma ligand. Two molecules of F-L-Leu bind to the ligand binding domain of a single PPARgamma molecule, making its mode of receptor interaction distinct from that of other nuclear receptor ligands. F-L-Leu induces a particular allosteric configuration of PPARgamma...

journal_title：Molecular cell

authors： Rocchi S,Picard F,Vamecq J,Gelman L,Potier N,Zeyer D,Dubuquoy L,Bac P,Champy MF,Plunket KD,Leesnitzer LM,Blanchard SG,Desreumaux P,Moras D,Renaud JP,Auwerx J

更新日期：2001-10-01 00:00:00

abstract：:Nutrients are not only organic compounds fueling bioenergetics and biosynthesis, but also key chemical signals controlling growth and metabolism. Nutrients enormously impact the production of reactive oxygen species (ROS), which play essential roles in normal physiology and diseases. How nutrient signaling is integrat...

journal_title：Molecular cell

authors： Tsang CK,Chen M,Cheng X,Qi Y,Chen Y,Das I,Li X,Vallat B,Fu LW,Qian CN,Wang HY,White E,Burley SK,Zheng XFS

更新日期：2018-05-03 00:00:00

abstract：:Three introns whose splicing is activated during meiosis in S. cerevisiae contain a Mer1p-dependent splicing enhancer. The enhancer can impose Mer1p-activated splicing upon the constitutively spliced actin intron provided the basal splicing efficiency of actin is first reduced. Of several nonessential splicing factors...

journal_title：Molecular cell

authors： Spingola M,Ares M Jr

更新日期：2000-08-01 00:00:00

abstract：:The selective degradation of intracellular components by autophagy involves sequential interactions of the cargo with a receptor, which also binds the autophagosomal protein Atg8 and a scaffold protein. Here, we demonstrated that mutations in C. elegans epg-11, which encodes an arginine methyltransferase homologous to...

journal_title：Molecular cell

authors： Li S,Yang P,Tian E,Zhang H

更新日期：2013-11-07 00:00:00

abstract：:Centromeres play several important roles in ensuring proper chromosome segregation. Not only do they promote kinetochore assembly for microtubule attachment, but they also support robust sister chromatid cohesion at pericentromeres and facilitate replication of centromeric DNA early in S phase. However, it is still el...

journal_title：Molecular cell

authors： Natsume T,Müller CA,Katou Y,Retkute R,Gierliński M,Araki H,Blow JJ,Shirahige K,Nieduszynski CA,Tanaka TU

更新日期：2013-06-06 00:00:00

abstract：:In vitro, the anaphase-promoting complex (APC) E3 ligase functions with E2 ubiquitin-conjugating enzymes of the E2-C and Ubc4/5 families to ubiquitinate substrates. However, only the use of the E2-C family, notably UbcH10, is genetically well validated. Here, we biochemically demonstrate preferential use of UbcH10 by ...

journal_title：Molecular cell

authors： Summers MK,Pan B,Mukhyala K,Jackson PK

更新日期：2008-08-22 00:00:00

abstract：:Signaling by TGFbeta regulates the expression of hundreds of genes. The rapid repression of c-Myc stands out because of its roles in growth control and cancer. Recent work shows that c-Myc repression by TGFbeta is mediated by the nuclear translocation of a novel, preformed complex composed of Smad3, E2F4 or E2F5, and ...

journal_title：Molecular cell

authors： Kowalik TF

更新日期：2002-07-01 00:00:00

abstract：:Cryo-EM structures of Dot1L in complex with a ubiquitinated nucleosome provide the long-sought-after molecular mechanism of Dot1L-mediated methylation of lysine 79 in histone H3 and explain crosstalk with histone H2B ubiquitination. ...

journal_title：Molecular cell

authors： Zhang Y,Kutateladze TG

更新日期：2019-06-06 00:00:00

abstract：:Microtubule plus end binding proteins (+TIPs) localize to the dynamic plus ends of microtubules, where they stimulate microtubule growth and recruit signaling molecules. Three main +TIP classes have been identified (XMAP215, EB1, and CLIP-170), but whether they act upon microtubule plus ends through a similar mechanis...

journal_title：Molecular cell

authors： Slep KC,Vale RD

更新日期：2007-09-21 00:00:00

abstract：:Aberrant folding and fibrillar aggregation by polyglutamine (polyQ) expansion proteins are associated with cytotoxicity in Huntington's disease and other neurodegenerative disorders. Hsp70 chaperones have an inhibitory effect on fibril formation and can alleviate polyQ cytotoxicity. Here we show that the cytosolic cha...

journal_title：Molecular cell

authors： Behrends C,Langer CA,Boteva R,Böttcher UM,Stemp MJ,Schaffar G,Rao BV,Giese A,Kretzschmar H,Siegers K,Hartl FU

更新日期：2006-09-15 00:00:00

abstract：:Formation and maintenance of specialized plasma membrane domains are crucial for many biological processes, such as cell polarization and signaling. During isotropic bud growth, the yeast cell periphery is divided into two domains: the bud surface, an active site of exocytosis and growth, and the relatively quiescent ...

journal_title：Molecular cell

authors： Barral Y,Mermall V,Mooseker MS,Snyder M

更新日期：2000-05-01 00:00:00

abstract：:mTOR senses nutrient and energy status to regulate cell survival and metabolism in response to environmental changes. Surprisingly, targeted mutation of Tsc1, a negative regulator of mTORC1, caused a broad reduction in miRNAs due to Drosha degradation. Conversely, targeted mutation of Raptor, an essential component of...

journal_title：Molecular cell

authors： Ye P,Liu Y,Chen C,Tang F,Wu Q,Wang X,Liu CG,Liu X,Liu R,Liu Y,Zheng P

更新日期：2015-02-19 00:00:00

abstract：:In this issue of Molecular Cell, Lee et al. (2009) report that SUMOylation of nuclear receptors LXRalpha and LXRbeta plays a critical role in the transrepression of IFN-gamma-induced STAT1-dependent inflammatory responses in brain astrocytes. ...

journal_title：Molecular cell

authors： Liu B,Shuai K

更新日期：2009-09-24 00:00:00