Abstract:
:In this study, we used Saccharomyces cerevisiae to identify a biological network that prevents the deleterious effects of endogenous reactive oxygen species. The absence of Tsa1, a key peroxiredoxin, caused increased rates of mutations, chromosomal rearrangements, and recombination. Defects in recombinational DNA double strand break repair, Rad6-mediated postreplicative repair, and DNA damage and replication checkpoints caused growth defects or lethality in the absence of Tsa1. In addition, the mutator phenotypes caused by a tsa1 mutation were significantly aggravated by defects in Ogg1, mismatch repair, or checkpoints. These results indicate that increased endogenous oxidative stress has broad effects on genome stability and is highly sensitive to the functional state of DNA repair and checkpoints. These findings may provide insight in understanding the consequences of various pathophysiological processes in regard to genomic instability.
journal_name
Mol Celljournal_title
Molecular cellauthors
Huang ME,Kolodner RDdoi
10.1016/j.molcel.2005.02.008subject
Has Abstractpub_date
2005-03-04 00:00:00pages
709-20issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(05)01085-3journal_volume
17pub_type
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