Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.

abstract：:The C-terminal fragment, Bb, of factor B combines with C3b to form the pivotal C3-convertase, C3bBb, of alternative complement pathway. Bb consists of a von Willebrand factor type A (vWFA) domain that is structurally similar to the I domains of integrins and a serine protease (SP) domain that is in inactive conformati...

journal_title：Molecular cell

authors： Ponnuraj K,Xu Y,Macon K,Moore D,Volanakis JE,Narayana SV

更新日期：2004-04-09 00:00:00

abstract：:The methylcytosine oxidase TET proteins play important roles in DNA demethylation and development. However, it remains elusive how exactly they target substrates and execute oxidation. Interestingly, we found that, in mice, the full-length TET1 isoform (TET1e) is restricted to early embryos, embryonic stem cells (ESCs...

journal_title：Molecular cell

authors： Zhang W,Xia W,Wang Q,Towers AJ,Chen J,Gao R,Zhang Y,Yen CA,Lee AY,Li Y,Zhou C,Liu K,Zhang J,Gu TP,Chen X,Chang Z,Leung D,Gao S,Jiang YH,Xie W

更新日期：2016-12-15 00:00:00

abstract：:All nuclear transcription is interrupted during mitosis. We examined the role of human TTF2, an RNA polymerase (Pol) I and II termination factor, in mitotic repression of transcription elongation. We find that TTF2 levels rise in the cytoplasm in S and G2 and at the onset of mitosis TTF2 translocates into the nucleus....

journal_title：Molecular cell

authors： Jiang Y,Liu M,Spencer CA,Price DH

更新日期：2004-05-07 00:00:00

abstract：:The interaction of transcription factors with target genes is highly dynamic. Whether the dynamic nature of these interactions is merely an intrinsic property of transcription factors or serves a regulatory role is unknown. Here we have used single-cell fluorescence imaging combined with computational modeling and chr...

journal_title：Molecular cell

authors： Gorski SA,Snyder SK,John S,Grummt I,Misteli T

更新日期：2008-05-23 00:00:00

abstract：:BRAF is an oncogenic protein kinase that drives cell growth and proliferation through the MEK-ERK signaling pathway. BRAF inhibitors have demonstrated antitumor efficacy in melanoma therapy but have also been found to be associated with the development of cutaneous squamous cell carcinomas (cSCCs) in certain patients....

journal_title：Molecular cell

authors： Shen CH,Yuan P,Perez-Lorenzo R,Zhang Y,Lee SX,Ou Y,Asara JM,Cantley LC,Zheng B

更新日期：2013-10-24 00:00:00

abstract：:The protective "cap" that assembles at chromosome ends recruits and controls an intricate network of biochemical activities, each one critical for telomere structure and the maintenance of genomic stability. Recent studies have uncovered the components of telomere caps and have started to define the pathways that lead...

journal_title：Molecular cell

authors： Ferreira MG,Miller KM,Cooper JP

更新日期：2004-01-16 00:00:00

abstract：:Most colorectal cancers (CRCs) containing activated BRAF (BRAF[V600E]) have a CpG island methylator phenotype (CIMP) characterized by aberrant hypermethylation of many genes, including the mismatch repair gene MLH1. MLH1 silencing results in microsatellite instability and a hypermutable phenotype. Through an RNAi scre...

journal_title：Molecular cell

authors： Fang M,Ou J,Hutchinson L,Green MR

更新日期：2014-09-18 00:00:00

abstract：:The Holliday junction is a central intermediate in homologous recombination. It consists of a four-way structure that can be resolved by cleavage to give either the crossover or noncrossover products observed. We show here that the formation of these products is controlled by the E. coli resolvasome (RuvABC) in such w...

journal_title：Molecular cell

authors： Cromie GA,Leach DR

更新日期：2000-10-01 00:00:00

abstract：:Previous studies suggested that expression of most yeast mRNAs is dominated by either transcription factor TFIID or SAGA. We re-examined the role of TFIID by rapid depletion of S. cerevisiae TFIID subunits and measurement of changes in nascent transcription. We find that transcription of nearly all mRNAs is strongly d...

journal_title：Molecular cell

authors： Warfield L,Ramachandran S,Baptista T,Devys D,Tora L,Hahn S

更新日期：2017-10-05 00:00:00

abstract：:The disulfide relay system in the intermembrane space of mitochondria is of crucial importance for mitochondrial biogenesis. Major players in this pathway are the oxidoreductase Mia40 that oxidizes substrates and the sulfhydryl oxidase Erv1 that reoxidizes Mia40. To analyze in detail the mechanism of this oxidative pa...

journal_title：Molecular cell

authors： Bien M,Longen S,Wagener N,Chwalla I,Herrmann JM,Riemer J

更新日期：2010-02-26 00:00:00

abstract：:Crossover recombination is essential for accurate chromosome segregation during meiosis. The MutSγ complex, Msh4-Msh5, facilitates crossing over by binding and stabilizing nascent recombination intermediates. We show that these activities are governed by regulated proteolysis. MutSγ is initially inactive for crossing ...

journal_title：Molecular cell

authors： He W,Rao HBDP,Tang S,Bhagwat N,Kulkarni DS,Ma Y,Chang MAW,Hall C,Bragg JW,Manasca HS,Baker C,Verhees GF,Ranjha L,Chen X,Hollingsworth NM,Cejka P,Hunter N

更新日期：2020-04-02 00:00:00

abstract：:In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied ini...

journal_title：Molecular cell

authors： Duchi D,Bauer DL,Fernandez L,Evans G,Robb N,Hwang LC,Gryte K,Tomescu A,Zawadzki P,Morichaud Z,Brodolin K,Kapanidis AN

更新日期：2016-09-15 00:00:00

abstract：:Perfringolysin O, a bacterial cytolytic toxin, forms unusually large pores in cholesterol-containing membranes by the spontaneous insertion of two of its four domains into the bilayer. By monitoring the kinetics of domain-specific conformational changes and pore formation using fluorescence spectroscopy, the temporal ...

journal_title：Molecular cell

authors： Heuck AP,Hotze EM,Tweten RK,Johnson AE

更新日期：2000-11-01 00:00:00

abstract：:Cas9 is a prokaryotic RNA-guided DNA endonuclease that binds substrates tightly in vitro but turns over rapidly when used to manipulate genomes in eukaryotic cells. Little is known about the factors responsible for dislodging Cas9 or how they influence genome engineering. Unbiased detection through proximity labeling ...

journal_title：Molecular cell

authors： Wang AS,Chen LC,Wu RA,Hao Y,McSwiggen DT,Heckert AB,Richardson CD,Gowen BG,Kazane KR,Vu JT,Wyman SK,Shin JJ,Darzacq X,Walter JC,Corn JE

更新日期：2020-07-16 00:00:00

abstract：:Autophagic turnover of intracellular constituents is critical for cellular housekeeping, nutrient recycling, and various aspects of growth and development in eukaryotes. Here we show that autophagy impacts the other major degradative route involving the ubiquitin-proteasome system by eliminating 26S proteasomes, a pro...

journal_title：Molecular cell

authors： Marshall RS,Li F,Gemperline DC,Book AJ,Vierstra RD

更新日期：2015-06-18 00:00:00

abstract：:In this issue of Molecular Cell, Guo et al. (2014) report that misfolded or aggregated nuclear proteins, such as pathogenic polyQ proteins, are cleared by a SUMO-dependent quality control pathway, which involves the E3 SUMO ligase PML and the SUMO-targeted ubiquitin ligase RNF4. ...

journal_title：Molecular cell

authors： Gärtner A,Muller S

更新日期：2014-07-03 00:00:00

abstract：:Mitochondrial protein import stress compromises functioning of the organelles, due to inadequate supply of inner mitochondrial proteins. Weidberg and Amon (2018) describe a new monitoring pathway in budding yeast, which restores mitochondrial function following the clearing of accumulated unfolded pre-transported mito...

journal_title：Molecular cell

authors： Desai R,Campanella M

更新日期：2018-07-05 00:00:00

abstract：:Protein kinase RNA-activated (PKR) induces immune response by sensing viral double-stranded RNAs (dsRNAs). However, growing evidence suggests that PKR can also be activated by endogenously expressed dsRNAs. Here, we capture these dsRNAs by formaldehyde-mediated crosslinking and immunoprecipitation sequencing and find ...

journal_title：Molecular cell

authors： Kim Y,Park J,Kim S,Kim M,Kang MG,Kwak C,Kang M,Kim B,Rhee HW,Kim VN

更新日期：2018-09-20 00:00:00

abstract：:E1 enzymes activate ubiquitin or related proteins and pass them to E2 enzymes. A recent structure and associated biochemical studies (Huang et al., 2005) show how an E1 binds its cognate E2 and indicates that large, conformational changes will be an integral component of the E1 reaction cycle. ...

journal_title：Molecular cell

authors： VanDemark AP,Hill CP

更新日期：2005-02-18 00:00:00

abstract：:X chromosome inactivation (XCI) depends on a noncoding sense-antisense transcript pair, Xist and Tsix. At the onset of XCI, Xist RNA accumulates on one of two Xs, coating and silencing the chromosome in cis. The molecular basis for monoallelic Xist upregulation is not known, though evidence predominantly supports a po...

journal_title：Molecular cell

authors： Sun BK,Deaton AM,Lee JT

更新日期：2006-03-03 00:00:00

abstract：:Linkage-specific polyubiquitin recognition is thought to make possible the diverse set of functional outcomes associated with ubiquitination. Thus far, mechanistic insight into this selectivity has been largely limited to single domains that preferentially bind to lysine 48-linked polyubiquitin (K48-polyUb) in isolati...

journal_title：Molecular cell

authors： Sims JJ,Cohen RE

更新日期：2009-03-27 00:00:00

abstract：:We used computational algorithms to find conserved sequences in the 3' untranslated region (UTR) of transcripts that exhibited rapid decay in primary human T cells and found that the consensus sequence UGUUUGUUUGU, which we have termed a GU-rich element (GRE), was enriched in short-lived transcripts. Using a tet-off r...

journal_title：Molecular cell

authors： Vlasova IA,Tahoe NM,Fan D,Larsson O,Rattenbacher B,Sternjohn JR,Vasdewani J,Karypis G,Reilly CS,Bitterman PB,Bohjanen PR

更新日期：2008-02-01 00:00:00

abstract：:RING ubiquitin ligases (E3) recruit ubiquitin-conjugate enzymes (E2) charged with ubiquitin (Ub) to catalyze ubiquitination. Non-covalent Ub binding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains elusive. Here, we show that backside bound Ub (Ub(B)) enhances both RING-in...

journal_title：Molecular cell

authors： Buetow L,Gabrielsen M,Anthony NG,Dou H,Patel A,Aitkenhead H,Sibbet GJ,Smith BO,Huang DT

更新日期：2015-04-16 00:00:00

abstract：:The interaction between pRB and E2F is critical for control of the cell cycle and apoptosis. Here we report that pRB contains two distinct E2F binding sites. The previously identified E2F binding site on pRB is necessary for stable association with E2Fs on DNA. A second E2F interaction site is located entirely within ...

journal_title：Molecular cell

authors： Dick FA,Dyson N

更新日期：2003-09-01 00:00:00

abstract：:RNA-binding proteins coordinate the fates of multiple RNAs, but the principles underlying these global interactions remain poorly understood. We elucidated regulatory mechanisms of the RNA-binding protein HuR, by integrating data from diverse high-throughput targeting technologies, specifically PAR-CLIP, RIP-chip, and...

journal_title：Molecular cell

authors： Mukherjee N,Corcoran DL,Nusbaum JD,Reid DW,Georgiev S,Hafner M,Ascano M Jr,Tuschl T,Ohler U,Keene JD

更新日期：2011-08-05 00:00:00

abstract：:The crystal structure of a heterodimer between the ligand-binding domains (LBDs) of the human RARalpha bound to a selective antagonist and the constitutively active mouse RXRalphaF318A mutant shows that, pushed by a bulky extension of the ligand, RARalpha helix H12 adopts an antagonist position. The unexpected presenc...

journal_title：Molecular cell

authors： Bourguet W,Vivat V,Wurtz JM,Chambon P,Gronemeyer H,Moras D

更新日期：2000-02-01 00:00:00

abstract：:Recently, a novel family of TATA binding protein (TBP)-like factors (TLFs) have been described in metazoan organisms; however, their function has not yet been elucidated. Using Caenorhabditis elegans (Ce) as a model, we demonstrate that CeTLF is required in vivo for zygotic transcription during embryogenesis. Eliminat...

journal_title：Molecular cell

authors： Dantonel JC,Quintin S,Lakatos L,Labouesse M,Tora L

更新日期：2000-09-01 00:00:00

abstract：:In Saccharomyces cerevisiae, methylation of histone H3 at active genes is an epigenetic mark that distinguishes active from silent chromatin and functions as a short-term "memory" of recent transcription. Methylation of H3 at lysine residues K4 and K79 depends on ubiquitylation of histone H2B, but the mechanisms linki...

journal_title：Molecular cell

authors： Ezhkova E,Tansey WP

更新日期：2004-02-13 00:00:00

abstract：:Using improved techniques, Baptista et al. (2017) and Warfield et al. (2017) revisit fundamental questions about SAGA and TFIID function in yeast. They conclude that each complex independently contributes to the expression of all genes transcribed by RNA polymerase II. ...

journal_title：Molecular cell

authors： Taatjes DJ

更新日期：2017-10-05 00:00:00

abstract：:In mammalian cells, histone deacetylase (HDAC) and Sirtuin (SIRT) are two families responsible for removing acetyl groups from acetylated proteins. Here, we describe protein deacetylation coupled with deacetylimination as a function of lysyl oxidase (LOX) family members. LOX-like 3 (Loxl3) associates with Stat3 in the...

journal_title：Molecular cell

authors： Ma L,Huang C,Wang XJ,Xin DE,Wang LS,Zou QC,Zhang YS,Tan MD,Wang YM,Zhao TC,Chatterjee D,Altura RA,Wang C,Xu YS,Yang JH,Fan YS,Han BH,Si J,Zhang X,Cheng J,Chang Z,Chin YE

更新日期：2017-01-19 00:00:00