Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase.

abstract：:Transcript-selective translational regulation of epithelial-mesenchymal transition (EMT) by transforming growth factor-β (TGF-β) is directed by the hnRNP E1-containing TGF-β-activated-translational (BAT) mRNP complex. Herein, eukaryotic elongation factor-1 A1 (eEF1A1) is identified as an integral component of the BAT ...

journal_title：Molecular cell

authors： Hussey GS,Chaudhury A,Dawson AE,Lindner DJ,Knudsen CR,Wilce MC,Merrick WC,Howe PH

更新日期：2011-02-18 00:00:00

abstract：:Identifying DNA fragile sites is crucial to reveal hotspots of genomic rearrangements, yet their precise mapping has been a challenge. A new study in this issue of Molecular Cell (Canela et al., 2016) introduces a highly sensitive and accurate method to detect DNA breaks in vivo that can be adapted to various experime...

journal_title：Molecular cell

authors： Banerjee U,Soutoglou E

更新日期：2016-09-01 00:00:00

abstract：:One of the earliest steps in metazoan pre-mRNA splicing involves binding of U2 snRNP auxiliary factor (U2AF) 65 KDa subunit to the polypyrimidine (Py) tract and of the 35 KDa subunit to the invariant AG dinucleotide at the intron 3' end. Here we use in vitro and in vivo depletion, as well as reconstitution assays usin...

journal_title：Molecular cell

authors： Tavanez JP,Madl T,Kooshapur H,Sattler M,Valcárcel J

更新日期：2012-02-10 00:00:00

abstract：:The transcriptional coactivators CBP and p300 are critical regulators of metazoan gene expression. They associate with many different DNA-bound transcription factors through small, conserved domains. We have identified a compactly folded 46 residue domain in CBP and p300, the IRF-3 binding domain (IBiD), and we have d...

journal_title：Molecular cell

authors： Lin CH,Hare BJ,Wagner G,Harrison SC,Maniatis T,Fraenkel E

更新日期：2001-09-01 00:00:00

abstract：:The stimulation of fibroblast growth factor receptors (FGFRs) with distinct FGF ligands generates specific cellular responses. However, the mechanisms underlying this paradigm have remained elusive. Here, we show that FGF-7 stimulation leads to FGFR2b degradation and, ultimately, cell proliferation, whereas FGF-10 pro...

journal_title：Molecular cell

authors： Francavilla C,Rigbolt KT,Emdal KB,Carraro G,Vernet E,Bekker-Jensen DB,Streicher W,Wikström M,Sundström M,Bellusci S,Cavallaro U,Blagoev B,Olsen JV

更新日期：2013-09-26 00:00:00

abstract：:S-adenosylmethionine (SAM) is the methyl-donor substrate for DNA and histone methyltransferases that regulate epigenetic states and subsequent gene expression. This metabolism-epigenome link sensitizes chromatin methylation to altered SAM abundance, yet the mechanisms that allow organisms to adapt and protect epigenet...

journal_title：Molecular cell

authors： Haws SA,Yu D,Ye C,Wille CK,Nguyen LC,Krautkramer KA,Tomasiewicz JL,Yang SE,Miller BR,Liu WH,Igarashi K,Sridharan R,Tu BP,Cryns VL,Lamming DW,Denu JM

更新日期：2020-04-16 00:00:00

abstract：:DNA double-strand breaks (DSBs) arise during physiological transcription, DNA replication, and antigen receptor diversification. Mistargeting or misprocessing of DSBs can result in pathological structural variation and mutation. Here we describe a sensitive method (END-seq) to monitor DNA end resection and DSBs genome...

journal_title：Molecular cell

authors： Canela A,Sridharan S,Sciascia N,Tubbs A,Meltzer P,Sleckman BP,Nussenzweig A

更新日期：2016-09-01 00:00:00

abstract：:The S. cerevisiae SCFCdc4p ubiquitin-protein ligase complex promotes cell cycle transitions through degradation of cell cycle regulators. To investigate SCFCdc4p regulation in vivo, we examined the stability of individual SCFCdc4p components. Whereas Cdc53p and Skp1p were stable, Cdc4p, the F box-containing component ...

journal_title：Molecular cell

authors： Zhou P,Howley PM

更新日期：1998-11-01 00:00:00

abstract：:The sliding clamp, PCNA, of the archaeon Sulfolobus solfataricus P2 is a heterotrimer of three distinct subunits (PCNA1, 2, and 3) that assembles in a defined manner. The PCNA heterotrimer, but not individual subunits, stimulates the activities of the DNA polymerase, DNA ligase I, and the flap endonuclease (FEN1) of S...

journal_title：Molecular cell

authors： Dionne I,Nookala RK,Jackson SP,Doherty AJ,Bell SD

更新日期：2003-01-01 00:00:00

abstract：:Ubiquitin (UB)-driven signaling systems permeate biology, and are often integrated with other types of post-translational modifications (PTMs), including phosphorylation. Flux through such pathways is dictated by the fractional stoichiometry of distinct modifications and protein assemblies as well as the spatial organ...

journal_title：Molecular cell

authors： Ordureau A,Münch C,Harper JW

更新日期：2015-05-21 00:00:00

abstract：:The ability to measure human aging from molecular profiles has practical implications in many fields, including disease prevention and treatment, forensics, and extension of life. Although chronological age has been linked to changes in DNA methylation, the methylome has not yet been used to measure and compare human ...

journal_title：Molecular cell

authors： Hannum G,Guinney J,Zhao L,Zhang L,Hughes G,Sadda S,Klotzle B,Bibikova M,Fan JB,Gao Y,Deconde R,Chen M,Rajapakse I,Friend S,Ideker T,Zhang K

更新日期：2013-01-24 00:00:00

abstract：:The formation of mRNPs controls the interaction of the translation and degradation machinery with individual mRNAs. The yeast Scd6 protein and its orthologs regulate translation and mRNA degradation in yeast, C. elegans, D. melanogaster, and humans by an unknown mechanism. We demonstrate that Scd6 represses translatio...

journal_title：Molecular cell

authors： Rajyaguru P,She M,Parker R

更新日期：2012-01-27 00:00:00

abstract：:In this issue of Molecular Cell,Sharma et al. (2019) show that normal cell growth requires conversion of an arginine residue in the RNA polymerase II C-terminal domain (CTD) to citrulline, uncovering a potential regulatory pathway involving opposing arginine modifications. ...

journal_title：Molecular cell

authors： Corden JL

更新日期：2019-01-03 00:00:00

abstract：:Wu et al. (2019) establish that the active zone proteins RIM and RIM-BP undergo liquid-liquid phase separation to tether Ca2+ channels. This important finding sets a new framework to study assembly and function of the presynaptic nerve terminal. ...

journal_title：Molecular cell

authors： Emperador-Melero J,Kaeser PS

更新日期：2019-03-07 00:00:00

abstract：:Canonical Wnt signaling is thought to regulate cell behavior mainly by inducing β-catenin-dependent transcription of target genes. In proliferating cells Wnt signaling peaks in the G2/M phase of the cell cycle, but the significance of this "mitotic Wnt signaling" is unclear. Here we introduce Wnt-dependent stabilizati...

journal_title：Molecular cell

authors： Acebron SP,Karaulanov E,Berger BS,Huang YL,Niehrs C

更新日期：2014-05-22 00:00:00

abstract：:The RNA exosome complex associates with nuclear and cytoplasmic cofactors to mediate the decay, surveillance, or processing of a wide variety of transcripts. In the cytoplasm, the conserved core of the exosome (Exo10) functions together with the conserved Ski complex. The interaction of S. cerevisiae Exo10 and Ski is ...

journal_title：Molecular cell

authors： Kowalinski E,Kögel A,Ebert J,Reichelt P,Stegmann E,Habermann B,Conti E

更新日期：2016-07-07 00:00:00

abstract：:Mirtrons are alternative precursors for microRNA biogenesis that were recently described in invertebrates. These short hairpin introns use splicing to bypass Drosha cleavage, which is otherwise essential for the generation of canonical animal microRNAs. Using computational and experimental strategies, we now establish...

journal_title：Molecular cell

authors： Berezikov E,Chung WJ,Willis J,Cuppen E,Lai EC

更新日期：2007-10-26 00:00:00

abstract：:Epstein-Barr virus (EBV) causes infectious mononucleosis, establishes long-term latent infections, and is associated with a variety of human tumors. The EBV gp42 glycoprotein binds MHC class II molecules, playing a critical role in infection of B lymphocytes. EBV gp42 belongs to the C-type lectin superfamily, with hom...

journal_title：Molecular cell

authors： Mullen MM,Haan KM,Longnecker R,Jardetzky TS

更新日期：2002-02-01 00:00:00

abstract：:BIK protein is an initiator of mitochondrial apoptosis, and BIK expression is induced by proapoptotic signals, including DNA damage. Here, we demonstrate that 3' end processing and expression of BIK mRNA are controlled by the nuclear PI4,5P(2)-regulated poly(A) polymerase Star-PAP downstream of DNA damage. Nuclear PKC...

journal_title：Molecular cell

authors： Li W,Laishram RS,Ji Z,Barlow CA,Tian B,Anderson RA

更新日期：2012-01-13 00:00:00

abstract：:Eukaryotic protein kinases are generally classified as being either tyrosine or serine-threonine specific. Though not evident from inspection of their primary sequences, many serine-threonine kinases display a significant preference for serine or threonine as the phosphoacceptor residue. Here we show that a residue lo...

journal_title：Molecular cell

authors： Chen C,Ha BH,Thévenin AF,Lou HJ,Zhang R,Yip KY,Peterson JR,Gerstein M,Kim PM,Filippakopoulos P,Knapp S,Boggon TJ,Turk BE

更新日期：2014-01-09 00:00:00

abstract：:Ubiquitination typically occurs through the sequential action of three enzymes catalyzing ubiquitin activation (E1), conjugation (E2), and ligation (E3) and regulates diverse eukaryotic cellular processes. Although monoubiquitination commonly confers nondegradative activities, mechanisms underlying its temporal and sp...

journal_title：Molecular cell

authors： Sun L,Fang J

更新日期：2016-06-16 00:00:00

abstract：:Nucleosome loss from a promoter region has recently been described as a potential mechanism for transcriptional regulation. We investigated whether H3/H4 histone chaperones mediate the loss of nucleosomes from the promoter of the yeast PHO5 gene during transcriptional activation. We found that antisilencing function 1...

journal_title：Molecular cell

authors： Adkins MW,Howar SR,Tyler JK

更新日期：2004-06-04 00:00:00

abstract：:In yeast, histone H3/H4 exchange independent of replication is poorly understood. Here, we analyzed the deposition of histone H3 molecules, synthesized during G1, using a high-density microarray histone exchange assay. While we found that H3 exchange in coding regions requires high levels of transcription, promoters e...

journal_title：Molecular cell

authors： Rufiange A,Jacques PE,Bhat W,Robert F,Nourani A

更新日期：2007-08-03 00:00:00

abstract：:The selective degradation of intracellular components by autophagy involves sequential interactions of the cargo with a receptor, which also binds the autophagosomal protein Atg8 and a scaffold protein. Here, we demonstrated that mutations in C. elegans epg-11, which encodes an arginine methyltransferase homologous to...

journal_title：Molecular cell

authors： Li S,Yang P,Tian E,Zhang H

更新日期：2013-11-07 00:00:00

abstract：:RNA editing in the mitochondria of kinetoplastids involves the addition and deletion of uridines at specific sites as directed by guide RNAs (gRNAs). Ample evidence shows that ribonucleoprotein (RNP) complexes carry out this posttranscriptional processing. One component of RNA editing complexes is REAP-1, a protein of...

journal_title：Molecular cell

authors： Madison-Antenucci S,Hajduk SL

更新日期：2001-04-01 00:00:00

abstract：:The interaction between pRB and E2F is critical for control of the cell cycle and apoptosis. Here we report that pRB contains two distinct E2F binding sites. The previously identified E2F binding site on pRB is necessary for stable association with E2Fs on DNA. A second E2F interaction site is located entirely within ...

journal_title：Molecular cell

authors： Dick FA,Dyson N

更新日期：2003-09-01 00:00:00

abstract：:The response to endoplasmic reticulum (ER) stress relies on activation of unfolded protein response (UPR) sensors, and the outcome of the UPR depends on the duration and strength of signal. Here, we demonstrate a mechanism that attenuates the activity of the UPR sensor inositol-requiring enzyme 1α (IRE1α). A resident ...

journal_title：Molecular cell

authors： Eletto D,Eletto D,Dersh D,Gidalevitz T,Argon Y

更新日期：2014-02-20 00:00:00

abstract：:Phospholipase C (PLC) isozymes are directly activated by heterotrimeric G proteins and Ras-like GTPases to hydrolyze phosphatidylinositol 4,5-bisphosphate into the second messengers diacylglycerol and inositol 1,4,5-trisphosphate. Although PLCs play central roles in myriad signaling cascades, the molecular details of ...

journal_title：Molecular cell

authors： Hicks SN,Jezyk MR,Gershburg S,Seifert JP,Harden TK,Sondek J

更新日期：2008-08-08 00:00:00

abstract：:Peptide release on the ribosome is catalyzed in the large subunit peptidyl transferase center by release factors on recognition of stop codons in the small subunit decoding center. Here we examine the role of the decoding center in this process. Mutation of decoding center nucleotides or removal of 2'OH groups from th...

journal_title：Molecular cell

authors： Youngman EM,He SL,Nikstad LJ,Green R

更新日期：2007-11-30 00:00:00

abstract：:Stress granules (SGs) are cytoplasmic assemblies of mRNPs stalled in translation initiation. They are induced by various stress conditions, including exposure to the environmental toxin and carcinogen arsenic. While perturbed SG turnover is linked to the pathogenesis of neurodegenerative diseases, the molecular mechan...

journal_title：Molecular cell

authors： Turakhiya A,Meyer SR,Marincola G,Böhm S,Vanselow JT,Schlosser A,Hofmann K,Buchberger A

更新日期：2018-06-07 00:00:00