Liquid Active Zones for Controlling the Phases of Synaptic Transmission.

abstract：:The transcriptional coactivators CBP and p300 are critical regulators of metazoan gene expression. They associate with many different DNA-bound transcription factors through small, conserved domains. We have identified a compactly folded 46 residue domain in CBP and p300, the IRF-3 binding domain (IBiD), and we have d...

journal_title：Molecular cell

authors： Lin CH,Hare BJ,Wagner G,Harrison SC,Maniatis T,Fraenkel E

更新日期：2001-09-01 00:00:00

abstract：:Autophagy constitutes a prominent mechanism through which eukaryotic cells preserve homeostasis in baseline conditions and in response to perturbations of the intracellular or extracellular microenvironment. Autophagic responses can be relatively non-selective or target a specific subcellular compartment. At least in ...

journal_title：Molecular cell

authors： Sica V,Galluzzi L,Bravo-San Pedro JM,Izzo V,Maiuri MC,Kroemer G

更新日期：2015-08-20 00:00:00

abstract：:In this issue of Molecular Cell, Ginno et al. (2012) describe unusual sequence features at promoter CpG islands that can lead to formation of persistent RNA-DNA hybrids (R loops), which are proposed to prevent genomic DNA methylation. ...

journal_title：Molecular cell

authors： Vertino PM,Wade PA

更新日期：2012-03-30 00:00:00

abstract：:The presence of microbial or self DNA in the cytoplasm of mammalian cells is a danger signal detected by the DNA sensor cyclic-GMP-AMP (cGAMP) synthase (cGAS), which catalyzes the production of cGAMP that in turn serves as a second messenger to activate innate immune responses. Here we show that endogenous cGAMP in ma...

journal_title：Molecular cell

authors： Zhang X,Shi H,Wu J,Zhang X,Sun L,Chen C,Chen ZJ

更新日期：2013-07-25 00:00:00

abstract：:Ribosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering poorly understood large-scale structural transitions that we analyzed by cryo-electron microscopy. Two nuclear pre-60S inte...

journal_title：Molecular cell

authors： Kater L,Mitterer V,Thoms M,Cheng J,Berninghausen O,Beckmann R,Hurt E

更新日期：2020-08-20 00:00:00

abstract：:Faithful propagation of functionally distinct chromatin states is crucial for maintaining cellular identity, and its breakdown can lead to diseases such as cancer. Whereas mechanisms that sustain repressed states have been intensely studied, regulatory circuits that protect active chromatin from inactivating signals a...

journal_title：Molecular cell

authors： Flury V,Georgescu PR,Iesmantavicius V,Shimada Y,Kuzdere T,Braun S,Bühler M

更新日期：2017-07-20 00:00:00

abstract：:The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA). The transcriptional coactivator CPB/p300 associates with NF-kappa B p65 through two sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, an...

journal_title：Molecular cell

authors： Zhong H,Voll RE,Ghosh S

更新日期：1998-04-01 00:00:00

abstract：:The RSC chromatin remodeler slides and ejects nucleosomes, utilizing a catalytic subunit (Sth1) with DNA translocation activity, which can pump DNA around the nucleosome. A central question is whether and how DNA translocation is regulated to achieve sliding versus ejection. Here, we report the regulation of DNA trans...

journal_title：Molecular cell

authors： Clapier CR,Kasten MM,Parnell TJ,Viswanathan R,Szerlong H,Sirinakis G,Zhang Y,Cairns BR

更新日期：2016-05-05 00:00:00

abstract：:Dong et al. (2017) establish how the mitochondrial Ca2+ uniporter (MCU) integrates Ca2+ and oxidative stress signals by identifying a cysteine residue that controls MCU channel activity, a mechanism causing mitochondrial Ca2+ overload and cell death during oxidative stress. ...

journal_title：Molecular cell

authors： Demaurex N,Rosselin M

更新日期：2017-03-16 00:00:00

abstract：:One fundamental function of telomeres is to prevent the ends of chromosomes from being sensed and treated as DNA damage. Here we present evidence for additional roles of telomeres in promoting proper chromosome segregation and DNA repair. We find that the fission yeast telomere protein Taz1p is required for cell cycle...

journal_title：Molecular cell

authors： Miller KM,Cooper JP

更新日期：2003-02-01 00:00:00

abstract：:Related coactivators p300 and CBP affect the transcriptional activities of many transcription factors (TF), producing multiple downstream effects. Here we show that immediate early response TF, Egr1, acts upstream of p300/CBP to induce or to repress transcription, depending on the stimulus. Cells induced with serum to...

journal_title：Molecular cell

authors： Yu J,de Belle I,Liang H,Adamson ED

更新日期：2004-07-02 00:00:00

abstract：:Ubiquitin-mediated proteolysis regulates the activity of diverse receptor systems. Here, we identify Smurf2, a C2-WW-HECT domain ubiquitin ligase and show that Smurf2 associates constitutively with Smad7. Smurf2 is nuclear, but binding to Smad7 induces export and recruitment to the activated TGF beta receptor, where i...

journal_title：Molecular cell

authors： Kavsak P,Rasmussen RK,Causing CG,Bonni S,Zhu H,Thomsen GH,Wrana JL

更新日期：2000-12-01 00:00:00

abstract：:RNA molecules play diverse functional roles in natural biological systems. There has been growing interest in designing synthetic RNA counterparts for programming biological function. The design of synthetic RNA molecules that exhibit diverse activities, including sensing, regulatory, information processing, and scaff...

journal_title：Molecular cell

authors： Liang JC,Bloom RJ,Smolke CD

更新日期：2011-09-16 00:00:00

abstract：:MazF and MazE are components of a chromosomal toxin-antitoxin system of Escherichia coli. In this issue of Molecular Cell, Kamada et al. describe the crystal structure of a MazE/MazF heterohexamer and propose that the mechanism of toxin-antidote recognition is common to other homologous chromosomal and plasmid-borne s...

journal_title：Molecular cell

authors： de la Cueva-Méndez G

更新日期：2003-04-01 00:00:00

abstract：:In this issue of Molecular Cell, Lu et al. (2019) analyze the role of the length and sequence complexity of the RNA polymerase II unstructured C-terminal domain in animal viability, development, and the dynamics of RNA polymerase II in vivo. ...

journal_title：Molecular cell

authors： Isaac RS,Churchman LS

更新日期：2019-03-21 00:00:00

abstract：:ATP is required for nucleation of actin filament branches by Arp2/3 complex, but the influence of ATP binding and hydrolysis are poorly understood. We determined crystal structures of bovine Arp2/3 complex cocrystallized with various bound adenine nucleotides and cations. Nucleotide binding favors closure of the nucle...

journal_title：Molecular cell

authors： Nolen BJ,Pollard TD

更新日期：2007-05-11 00:00:00

abstract：:Upon heterologous overexpression, many proteins misfold or aggregate, thus resulting in low functional yields. Human acetylcholinesterase (hAChE), an enzyme mediating synaptic transmission, is a typical case of a human protein that necessitates mammalian systems to obtain functional expression. We developed a computat...

journal_title：Molecular cell

authors： Goldenzweig A,Goldsmith M,Hill SE,Gertman O,Laurino P,Ashani Y,Dym O,Unger T,Albeck S,Prilusky J,Lieberman RL,Aharoni A,Silman I,Sussman JL,Tawfik DS,Fleishman SJ

更新日期：2016-07-21 00:00:00

abstract：:The essential histone H3 lysine 79 methyltransferase Dot1L regulates transcription and genomic stability and is deregulated in leukemia. The activity of Dot1L is stimulated by mono-ubiquitination of histone H2B on lysine 120 (H2BK120Ub); however, the detailed mechanism is not understood. We report cryo-EM structures o...

journal_title：Molecular cell

authors： Valencia-Sánchez MI,De Ioannes P,Wang M,Vasilyev N,Chen R,Nudler E,Armache JP,Armache KJ

更新日期：2019-06-06 00:00:00

abstract：:The small nuclear RNA (snRNA) genes have been widely used as a model system for understanding transcriptional regulation due to the unique aspects of their promoter structure, selectivity for either RNA polymerase (Pol) II or III, and because of their unique mechanism of termination that is tightly linked with the pro...

journal_title：Molecular cell

authors： Hu D,Smith ER,Garruss AS,Mohaghegh N,Varberg JM,Lin C,Jackson J,Gao X,Saraf A,Florens L,Washburn MP,Eissenberg JC,Shilatifard A

更新日期：2013-08-22 00:00:00

abstract：:In the death receptor induced apoptotic pathway, caspase-8 autocatalytically cleaves itself at specific cleavage sites. To better understand the regulatory mechanisms behind caspase-8 activation, we compared active wild-type caspase-8 (wtC8) and an uncleavable form of procaspase-8 (uncleavable C8). We demonstrate that...

journal_title：Molecular cell

authors： Donepudi M,Mac Sweeney A,Briand C,Grütter MG

更新日期：2003-02-01 00:00:00

abstract：:Heterochromatin is required to restrict aberrant expression of retrotransposons, but it remains poorly defined due to the underlying repeat-rich sequences. We dissected Suv39h-dependent histone H3 lysine 9 trimethylation (H3K9me3) by genome-wide ChIP sequencing in mouse embryonic stem cells (ESCs). Refined bioinformat...

journal_title：Molecular cell

authors： Bulut-Karslioglu A,De La Rosa-Velázquez IA,Ramirez F,Barenboim M,Onishi-Seebacher M,Arand J,Galán C,Winter GE,Engist B,Gerle B,O'Sullivan RJ,Martens JH,Walter J,Manke T,Lachner M,Jenuwein T

更新日期：2014-07-17 00:00:00

abstract：:c-Jun, a major transcription factor in the activating protein 1 (AP-1) family of regulatory proteins, is activated by many physiologic and pathologic stimuli. However, whether c-jun is regulated by epigenetic modification of chromatin structure is not clear. We showed here that c-jun was transcriptionally repressed in...

journal_title：Molecular cell

authors： Xia Y,Wang J,Liu TJ,Yung WK,Hunter T,Lu Z

更新日期：2007-01-26 00:00:00

abstract：:Steroid hormones have rapid nongenomic effects on cell-signaling pathways, but the receptor mechanisms responsible for this are not understood. We have identified a specific polyproline motif in the amino-terminal domain of conventional progesterone receptor (PR) that mediates direct progestin-dependent interaction of...

journal_title：Molecular cell

authors： Boonyaratanakornkit V,Scott MP,Ribon V,Sherman L,Anderson SM,Maller JL,Miller WT,Edwards DP

更新日期：2001-08-01 00:00:00

abstract：:Signal-induced alternative splicing of the CD45 gene in human T cells is essential for proper immune function. Skipping of the CD45 variable exons is controlled, in large part, by the recruitment of PSF to the pre-mRNA substrate upon T cell activation; however, the signaling cascade leading to exon exclusion has remai...

journal_title：Molecular cell

authors： Heyd F,Lynch KW

更新日期：2010-10-08 00:00:00

abstract：:Both splicing factors and microRNAs are important regulatory molecules that play key roles in posttranscriptional gene regulation. By miRNA deep sequencing, we identified 40 miRNAs that are differentially expressed upon ectopic overexpression of the splicing factor SF2/ASF. Here we show that SF2/ASF and one of its upr...

journal_title：Molecular cell

authors： Wu H,Sun S,Tu K,Gao Y,Xie B,Krainer AR,Zhu J

更新日期：2010-04-09 00:00:00

abstract：:Mutations within BRCA1 predispose carriers to a high risk of breast and ovarian cancers. BRCA1 functions to maintain genomic stability through the assembly of multiple protein complexes involved in DNA repair, cell-cycle arrest, and transcriptional regulation. Here, we report the identification of a DNA damage-induced...

journal_title：Molecular cell

authors： Savage KI,Gorski JJ,Barros EM,Irwin GW,Manti L,Powell AJ,Pellagatti A,Lukashchuk N,McCance DJ,McCluggage WG,Schettino G,Salto-Tellez M,Boultwood J,Richard DJ,McDade SS,Harkin DP

更新日期：2014-05-08 00:00:00

abstract：:Multicellular organisms have multiple homologs of the yeast ATG8 gene, but the differential roles of these homologs in autophagy during development remain largely unknown. Here we investigated structure/function relationships in the two C. elegans Atg8 homologs, LGG-1 and LGG-2. lgg-1 is essential for degradation of p...

journal_title：Molecular cell

authors： Wu F,Watanabe Y,Guo XY,Qi X,Wang P,Zhao HY,Wang Z,Fujioka Y,Zhang H,Ren JQ,Fang TC,Shen YX,Feng W,Hu JJ,Noda NN,Zhang H

更新日期：2015-12-17 00:00:00

abstract：:Increasing evidence suggests that tRNA levels are dynamically and specifically regulated in response to internal and external cues to modulate the cellular translational program. However, the molecular players and the mechanisms regulating the gene-specific expression of tRNAs are still unknown. Using an inducible aux...

journal_title：Molecular cell

authors： Gerber A,Ito K,Chu CS,Roeder RG

更新日期：2020-05-21 00:00:00

abstract：:Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts. We now show that Sen1 helicase possesses a wider function by restricting the occurrence of RNA:DNA hybrids that may naturally form dur...

journal_title：Molecular cell

authors： Mischo HE,Gómez-González B,Grzechnik P,Rondón AG,Wei W,Steinmetz L,Aguilera A,Proudfoot NJ

更新日期：2011-01-07 00:00:00

abstract：:Atg7 is a noncanonical, homodimeric E1 enzyme that interacts with the noncanonical E2 enzyme, Atg3, to mediate conjugation of the ubiquitin-like protein (UBL) Atg8 during autophagy. Here we report that the unique N-terminal domain of Atg7 (Atg7(NTD)) recruits a unique "flexible region" from Atg3 (Atg3(FR)). The struct...

journal_title：Molecular cell

authors： Taherbhoy AM,Tait SW,Kaiser SE,Williams AH,Deng A,Nourse A,Hammel M,Kurinov I,Rock CO,Green DR,Schulman BA

更新日期：2011-11-04 00:00:00