Construction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis.

Abstract:

:Ribosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering poorly understood large-scale structural transitions that we analyzed by cryo-electron microscopy. Two nuclear pre-60S intermediates were discovered that represent previously unknown states after Rea1-mediated removal of the Ytm1-Erb1 complex and reveal how the L1 stalk develops from a pre-mature nucleolar to a mature-like nucleoplasmic state. A later pre-60S intermediate shows how the central protuberance arises, assisted by the nearby Rix1-Rea1 machinery, which was solved in its pre-ribosomal context to molecular resolution. This revealed a Rix12-Ipi32 tetramer anchored to the pre-60S via Ipi1, strategically positioned to monitor this decisive remodeling. These results are consistent with a general underlying principle that temporarily stabilized immature RNA domains are successively remodeled by assembly factors, thereby ensuring failsafe assembly progression.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Kater L,Mitterer V,Thoms M,Cheng J,Berninghausen O,Beckmann R,Hurt E

doi

10.1016/j.molcel.2020.06.032

subject

Has Abstract

pub_date

2020-08-20 00:00:00

pages

615-628.e5

issue

4

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(20)30434-2

journal_volume

79

pub_type

杂志文章
  • Methylation of Histone H3K79 by Dot1L Requires Multiple Contacts with the Ubiquitinated Nucleosome.

    abstract::Cryo-EM structures of Dot1L in complex with a ubiquitinated nucleosome provide the long-sought-after molecular mechanism of Dot1L-mediated methylation of lysine 79 in histone H3 and explain crosstalk with histone H2B ubiquitination. ...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2019.05.013

    authors: Zhang Y,Kutateladze TG

    更新日期:2019-06-06 00:00:00

  • Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.

    abstract::The RNA modification N6-methyladenosine (m6A) modulates mRNA fate and thus affects many biological processes. We analyzed m6A across the transcriptome following infection by dengue virus (DENV), Zika virus (ZIKV), West Nile virus (WNV), and hepatitis C virus (HCV). We found that infection by these viruses in the Flavi...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2019.11.007

    authors: Gokhale NS,McIntyre ABR,Mattocks MD,Holley CL,Lazear HM,Mason CE,Horner SM

    更新日期:2020-02-06 00:00:00

  • Stay close to your sister.

    abstract::Yeast Sir2 protein regulates chromatin structure and suppresses recombination at the multiple tandem rDNA array. In the current issue of Cell, Kobayashi and colleagues reinvestigate rDNA dynamics in sir2 strains and find that Sir2 is necessary to recruit cohesins to the array, which influences the nature of rDNA recom...

    journal_title:Molecular cell

    pub_type: 杂志文章,评审

    doi:10.1016/s1097-2765(04)00266-7

    authors: Reid RJ,Rothstein R

    更新日期:2004-05-21 00:00:00

  • The Stress Granule Transcriptome Reveals Principles of mRNA Accumulation in Stress Granules.

    abstract::Stress granules are mRNA-protein assemblies formed from nontranslating mRNAs. Stress granules are important in the stress response and may contribute to some degenerative diseases. Here, we describe the stress granule transcriptome of yeast and mammalian cells through RNA-sequencing (RNA-seq) analysis of purified stre...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2017.10.015

    authors: Khong A,Matheny T,Jain S,Mitchell SF,Wheeler JR,Parker R

    更新日期:2017-11-16 00:00:00

  • Identification of a Ctcf cofactor, Yy1, for the X chromosome binary switch.

    abstract::In mammals, inactivation of one X chromosome in the female equalizes gene dosages between XX females and XY males. Two noncoding loci, Tsix and Xite, together regulate X chromosome fate by controlling homologous chromosome pairing, counting, and mutually exclusive choice. Following choice, the asymmetry of Xite and Ts...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2006.11.017

    authors: Donohoe ME,Zhang LF,Xu N,Shi Y,Lee JT

    更新日期:2007-01-12 00:00:00

  • Regulation of the histone H4 monomethylase PR-Set7 by CRL4(Cdt2)-mediated PCNA-dependent degradation during DNA damage.

    abstract::The histone methyltransferase PR-Set7/Set8 is the sole enzyme that catalyzes monomethylation of histone H4 at K20 (H4K20me1). Previous reports document disparate evidence regarding PR-Set7 expression during the cell cycle, the biological relevance of PR-Set7 interaction with PCNA, and its role in the cell. We find tha...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2010.10.011

    authors: Oda H,Hübner MR,Beck DB,Vermeulen M,Hurwitz J,Spector DL,Reinberg D

    更新日期:2010-11-12 00:00:00

  • Y14 and hUpf3b form an NMD-activating complex.

    abstract::Messenger RNAs with premature translation termination codons (PTCs) are degraded by nonsense-mediated mRNA decay (NMD). In mammals, PTCs are discriminated from physiological stop codons by a process thought to involve the splicing-dependent deposition of an exon junction complex (EJC), EJC-mediated recruitment of Upf3...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(03)00142-4

    authors: Gehring NH,Neu-Yilik G,Schell T,Hentze MW,Kulozik AE

    更新日期:2003-04-01 00:00:00

  • Applied force provides insight into transcriptional pausing and its modulation by transcription factor NusA.

    abstract::Transcriptional pausing by RNA polymerase (RNAP) plays an essential role in gene regulation. Pausing is modified by various elongation factors, including prokaryotic NusA, but the mechanisms underlying pausing and NusA function remain unclear. Alternative models for pausing invoke blockade events that precede transloc...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2011.09.018

    authors: Zhou J,Ha KS,La Porta A,Landick R,Block SM

    更新日期:2011-11-18 00:00:00

  • Just the FACTs: histone H2B ubiquitylation and nucleosome dynamics.

    abstract::In this issue of Molecular Cell, Fleming et al. (2008) show that histone H2B ubiquitylation and FACT function interdependently to facilitate nucleosome reassembly during transcription elongation, thereby demonstrating that histone posttranslational modifications can provide important but transient transcriptional sign...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2008.06.012

    authors: Hartzog GA,Quan TK

    更新日期:2008-07-11 00:00:00

  • PRC1 Catalytic Activity Is Central to Polycomb System Function.

    abstract::The Polycomb repressive system is an essential chromatin-based regulator of gene expression. Despite being extensively studied, how the Polycomb system selects its target genes is poorly understood, and whether its histone-modifying activities are required for transcriptional repression remains controversial. Here, we...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2019.12.001

    authors: Blackledge NP,Fursova NA,Kelley JR,Huseyin MK,Feldmann A,Klose RJ

    更新日期:2020-02-20 00:00:00

  • Mitophagy Controls the Activities of Tumor Suppressor p53 to Regulate Hepatic Cancer Stem Cells.

    abstract::Autophagy is required for benign hepatic tumors to progress into malignant hepatocellular carcinoma. However, the mechanism is unclear. Here, we report that mitophagy, the selective removal of mitochondria by autophagy, positively regulates hepatic cancer stem cells (CSCs) by suppressing the tumor suppressor p53. When...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2017.09.022

    authors: Liu K,Lee J,Kim JY,Wang L,Tian Y,Chan ST,Cho C,Machida K,Chen D,Ou JJ

    更新日期:2017-10-19 00:00:00

  • SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation.

    abstract::Activation of NF-kappaB is achieved by ubiquitination and proteasome-mediated degradation of IkappaBalpha. We have detected modified IkappaBalpha, conjugated to the small ubiquitin-like protein SUMO-1, which is resistant to signal-induced degradation. In the presence of an E1 SUMO-1-activating enzyme, Ubch9 conjugated...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(00)80133-1

    authors: Desterro JM,Rodriguez MS,Hay RT

    更新日期:1998-08-01 00:00:00

  • Smad proteins bind a conserved RNA sequence to promote microRNA maturation by Drosha.

    abstract::The signal transducers of the transforming growth factor beta (TGFbeta)/bone morphogenetic protein (BMP), the Smads, promote the expression of a subset of miRNAs by facilitating the cleavage reaction by Drosha. The mechanism that limits Smad-mediated processing to a selective group of miRNAs remained hitherto unexplor...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2010.07.011

    authors: Davis BN,Hilyard AC,Nguyen PH,Lagna G,Hata A

    更新日期:2010-08-13 00:00:00

  • Too sweet for autophagy: hexokinase inhibition of mTORC1 activates autophagy.

    abstract::In this issue, Roberts et al. (2014) describe how hexokinase and mTORC1 cooperate to sense disequilibrium between glucose uptake and utilization and direct the balance of anabolism and catabolism to ensure the appropriate use of cellular resources. ...

    journal_title:Molecular cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.molcel.2014.02.009

    authors: Kundu M

    更新日期:2014-02-20 00:00:00

  • Nuclear pore components are involved in the transcriptional regulation of dosage compensation in Drosophila.

    abstract::Dosage compensation in Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from Drosophila embryos, Schneider cells, and human HeLa cells. We ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2006.02.007

    authors: Mendjan S,Taipale M,Kind J,Holz H,Gebhardt P,Schelder M,Vermeulen M,Buscaino A,Duncan K,Mueller J,Wilm M,Stunnenberg HG,Saumweber H,Akhtar A

    更新日期:2006-03-17 00:00:00

  • Dynamics of enhancer-promoter communication during differentiation-induced gene activation.

    abstract::We analyzed the order of recruitment of factors to the HNF-4alpha regulatory regions upon the initial activation of the gene during enterocyte differentiation. An initially independent assembly of regulatory complexes at the proximal promoter and the upstream enhancer regions was followed by the tracking of the entire...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(02)00786-4

    authors: Hatzis P,Talianidis I

    更新日期:2002-12-01 00:00:00

  • Regulated Proteolysis of MutSγ Controls Meiotic Crossing Over.

    abstract::Crossover recombination is essential for accurate chromosome segregation during meiosis. The MutSγ complex, Msh4-Msh5, facilitates crossing over by binding and stabilizing nascent recombination intermediates. We show that these activities are governed by regulated proteolysis. MutSγ is initially inactive for crossing ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2020.02.001

    authors: He W,Rao HBDP,Tang S,Bhagwat N,Kulkarni DS,Ma Y,Chang MAW,Hall C,Bragg JW,Manasca HS,Baker C,Verhees GF,Ranjha L,Chen X,Hollingsworth NM,Cejka P,Hunter N

    更新日期:2020-04-02 00:00:00

  • TBP-like factor is required for embryonic RNA polymerase II transcription in C. elegans.

    abstract::Recently, a novel family of TATA binding protein (TBP)-like factors (TLFs) have been described in metazoan organisms; however, their function has not yet been elucidated. Using Caenorhabditis elegans (Ce) as a model, we demonstrate that CeTLF is required in vivo for zygotic transcription during embryogenesis. Eliminat...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(00)00069-1

    authors: Dantonel JC,Quintin S,Lakatos L,Labouesse M,Tora L

    更新日期:2000-09-01 00:00:00

  • Acetylation of MyoD directed by PCAF is necessary for the execution of the muscle program.

    abstract::p300/CBP and PCAF coactivators have acetyltransferase activities and regulate transcription, cell cycle progression, and differentiation. They are both required for MyoD activity and muscle differentiation. Nevertheless, their roles must be different since the acetyltransferase activity of PCAF but not of p300 is invo...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(00)80383-4

    authors: Sartorelli V,Puri PL,Hamamori Y,Ogryzko V,Chung G,Nakatani Y,Wang JY,Kedes L

    更新日期:1999-11-01 00:00:00

  • ZFAND1 Recruits p97 and the 26S Proteasome to Promote the Clearance of Arsenite-Induced Stress Granules.

    abstract::Stress granules (SGs) are cytoplasmic assemblies of mRNPs stalled in translation initiation. They are induced by various stress conditions, including exposure to the environmental toxin and carcinogen arsenic. While perturbed SG turnover is linked to the pathogenesis of neurodegenerative diseases, the molecular mechan...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2018.04.021

    authors: Turakhiya A,Meyer SR,Marincola G,Böhm S,Vanselow JT,Schlosser A,Hofmann K,Buchberger A

    更新日期:2018-06-07 00:00:00

  • A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif.

    abstract::HIV-1 Vpu interacts with CD4 in the endoplasmic reticulum and triggers CD4 degradation, presumably by proteasomes. Human beta TrCP identified by interaction with Vpu connects CD4 to this proteolytic machinery, and CD4-Vpu-beta TrCP ternary complexes have been detected by coimmunoprecipitation. beta TrCP binding to Vpu...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(00)80056-8

    authors: Margottin F,Bour SP,Durand H,Selig L,Benichou S,Richard V,Thomas D,Strebel K,Benarous R

    更新日期:1998-03-01 00:00:00

  • Vesicle Tethering on the Surface of Phase-Separated Active Zone Condensates.

    abstract::Tethering of synaptic vesicles (SVs) to the active zone determines synaptic strength, although the molecular basis governing SV tethering is elusive. Here, we discover that small unilamellar vesicles (SUVs) and SVs from rat brains coat on the surface of condensed liquid droplets formed by active zone proteins RIM, RIM...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2020.10.029

    authors: Wu X,Ganzella M,Zhou J,Zhu S,Jahn R,Zhang M

    更新日期:2021-01-07 00:00:00

  • High-Resolution Mapping of Multiway Enhancer-Promoter Interactions Regulating Pathogen Detection.

    abstract::Eukaryotic gene expression regulation involves thousands of distal regulatory elements. Understanding the quantitative contribution of individual enhancers to gene expression is critical for assessing the role of disease-associated genetic risk variants. Yet, we lack the ability to accurately link genes with their dis...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2020.09.005

    authors: Vangala P,Murphy R,Quinodoz SA,Gellatly K,McDonel P,Guttman M,Garber M

    更新日期:2020-10-15 00:00:00

  • Swi6/HP1 recruits a JmjC domain protein to facilitate transcription of heterochromatic repeats.

    abstract::Heterochromatin formation is generally thought to result in transcriptional repression of target loci. However, RNAi-mediated heterochromatin assembly requires RNA polymerase II (Pol II) transcription. The mechanism facilitating Pol II accessibility to heterochromatin is unknown. We show that the fission yeast Epe1, a...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2006.05.010

    authors: Zofall M,Grewal SI

    更新日期:2006-06-09 00:00:00

  • Brefeldin A acts to stabilize an abortive ARF-GDP-Sec7 domain protein complex: involvement of specific residues of the Sec7 domain.

    abstract::We demonstrate that the major in vivo targets of brefeldin A (BFA) in the secretory pathway of budding yeast are the three members of the Sec7 domain family of ARF exchange factors: Gea1p and Gea2p (functionally interchangeable) and Sec7p. Specific residues within the Sec7 domain are important for BFA inhibition of AR...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(00)80455-4

    authors: Peyroche A,Antonny B,Robineau S,Acker J,Cherfils J,Jackson CL

    更新日期:1999-03-01 00:00:00

  • MOF-regulated acetylation of MSL-3 in the Drosophila dosage compensation complex.

    abstract::Dosage compensation ensures equal expression of X-linked genes in males and females. In Drosophila, equalization is achieved by hypertranscription of the male X chromosome. This process requires an RNA/protein containing dosage compensation complex (DCC). Here we use RNA interference of individual DCC components to de...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(03)00140-0

    authors: Buscaino A,Köcher T,Kind JH,Holz H,Taipale M,Wagner K,Wilm M,Akhtar A

    更新日期:2003-05-01 00:00:00

  • A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends.

    abstract::The mechanisms by which telomeres are distinguished from DNA double-strand breaks are poorly understood. Here we have defined the minimal requirements for the protection of telomeric DNA ends from nonhomologous end-joining (NHEJ). Neither long, single-stranded overhangs nor t loop formation is essential to prevent NHE...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2007.03.023

    authors: Bae NS,Baumann P

    更新日期:2007-05-11 00:00:00

  • Phosphorylation of Tip60 by GSK-3 determines the induction of PUMA and apoptosis by p53.

    abstract::Activation of p53 by DNA damage results in either cell-cycle arrest, allowing DNA repair and cell survival, or induction of apoptosis. As these opposite outcomes are both mediated by p53 stabilization, additional mechanisms to determine this decision must exist. Here, we show that glycogen synthase kinase-3 (GSK-3) is...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2011.03.033

    authors: Charvet C,Wissler M,Brauns-Schubert P,Wang SJ,Tang Y,Sigloch FC,Mellert H,Brandenburg M,Lindner SE,Breit B,Green DR,McMahon SB,Borner C,Gu W,Maurer U

    更新日期:2011-06-10 00:00:00

  • p58(IPK)-mediated attenuation of the proapoptotic PERK-CHOP pathway allows malignant progression upon low glucose.

    abstract::As solid tumors expand, oxygen and nutrients become limiting owing to inadequate vascularization and diffusion. How malignant cells cope with this potentially lethal metabolic stress remains poorly understood. We found that glucose shortage associated with malignant progression triggers apoptosis through the endoplasm...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/j.molcel.2013.01.009

    authors: Huber AL,Lebeau J,Guillaumot P,Pétrilli V,Malek M,Chilloux J,Fauvet F,Payen L,Kfoury A,Renno T,Chevet E,Manié SN

    更新日期:2013-03-28 00:00:00

  • CREB binding protein recruitment to the transcription complex requires growth factor-dependent phosphorylation of its GF box.

    abstract::Growth factors such as epidermal growth factor (EGF) and insulin regulate development and metabolism via genes containing both POU homeodomain (Pit-1) and phorbol ester (AP-1) response elements. Although CREB binding protein (CBP) functions as a coactivator on these elements, the mechanism of transactivation was previ...

    journal_title:Molecular cell

    pub_type: 杂志文章

    doi:10.1016/s1097-2765(01)00202-7

    authors: Zanger K,Radovick S,Wondisford FE

    更新日期:2001-03-01 00:00:00