Distressing bacteria: structure of a prokaryotic detox program.

abstract：:Alternative splicing (AS) generates isoform diversity for cellular identity and homeostasis in multicellular life. Although AS variation has been observed among single cells, little is known about the biological or evolutionary significance of such variation. We developed Expedition, a computational framework consisti...

journal_title：Molecular cell

authors： Song Y,Botvinnik OB,Lovci MT,Kakaradov B,Liu P,Xu JL,Yeo GW

更新日期：2017-07-06 00:00:00

abstract：:The circadian clock is a self-sustaining oscillator that controls daily rhythms. For the proper circadian gene expression, dynamic changes in chromatin structure are important. Although chromatin modifiers have been shown to play a role in circadian gene expression, the in vivo role of circadian signal-modulated chrom...

journal_title：Molecular cell

authors： Nam HJ,Boo K,Kim D,Han DH,Choe HK,Kim CR,Sun W,Kim H,Kim K,Lee H,Metzger E,Schuele R,Yoo SH,Takahashi JS,Cho S,Son GH,Baek SH

更新日期：2014-03-06 00:00:00

abstract：:Cdh1 is a well-established activator of APC/C, a RING-type ubiquitin ligase. In this issue of Molecular Cell, Wan et al. (2011) report an APC/C-independent role of Cdh1 during development as an activator for Smurf1, a HECT-type ubiquitin ligase. ...

journal_title：Molecular cell

authors： Jia L,Yu H

更新日期：2011-12-09 00:00:00

abstract：:There are few tools available for dissecting and elucidating the functions of DNA satellites and other nongenic DNA. To address this, we have explored the experimental potential of DNA sequence-specific drugs containing pyrrole and imidazole amino acids (polyamides). Compounds were synthesized that target different Dr...

journal_title：Molecular cell

authors： Janssen S,Durussel T,Laemmli UK

更新日期：2000-11-01 00:00:00

abstract：:Proteins are translocated across membranes through a channel that is formed by the prokaryotic SecY or eukaryotic Sec61 complex. The crystal structure of the SecY channel from M. jannaschii revealed a plug domain that appears to seal the channel in its closed state. However, the role of the plug remains unclear, parti...

journal_title：Molecular cell

authors： Li W,Schulman S,Boyd D,Erlandson K,Beckwith J,Rapoport TA

更新日期：2007-05-25 00:00:00

abstract：:Genes are transcribed in a discontinuous pattern referred to as RNA bursting, but the mechanisms regulating this process are unclear. Although many physiological signals, including glucocorticoid hormones, are pulsatile, the effects of transient stimulation on bursting are unknown. Here we characterize RNA synthesis f...

journal_title：Molecular cell

authors： Stavreva DA,Garcia DA,Fettweis G,Gudla PR,Zaki GF,Soni V,McGowan A,Williams G,Huynh A,Palangat M,Schiltz RL,Johnson TA,Presman DM,Ferguson ML,Pegoraro G,Upadhyaya A,Hager GL

更新日期：2019-09-19 00:00:00

abstract：:In this issue of Molecular Cell, Gross et al. (2016) find a CpG DNA methylation signature in blood cells of patients with chronic well-controlled HIV infection that correlates with accelerated aging. ...

journal_title：Molecular cell

authors： Boulias K,Lieberman J,Greer EL

更新日期：2016-04-21 00:00:00

abstract：:The role of the protein kinase Akt in cell migration is incompletely understood. Here we show that sphingosine-1-phosphate (S1P)-induced endothelial cell migration requires the Akt-mediated phosphorylation of the G protein-coupled receptor (GPCR) EDG-1. Activated Akt binds to EDG-1 and phosphorylates the third intrace...

journal_title：Molecular cell

authors： Lee MJ,Thangada S,Paik JH,Sapkota GP,Ancellin N,Chae SS,Wu M,Morales-Ruiz M,Sessa WC,Alessi DR,Hla T

更新日期：2001-09-01 00:00:00

abstract：:The heat shock protein 90 (Hsp90) family of heat shock proteins is an abundantly expressed and highly conserved family of ATP-dependent molecular chaperones. Hsp90 facilitates remodeling and activation of hundreds of proteins. In this study, we developed a screen to identify Hsp90-defective mutants in E. coli. The mut...

journal_title：Molecular cell

authors： Genest O,Reidy M,Street TO,Hoskins JR,Camberg JL,Agard DA,Masison DC,Wickner S

更新日期：2013-02-07 00:00:00

abstract：:In a recent issue of Molecular Cell, Pidoux et al. (2009) and Williams et al. (2009) identify S. pombe Scm3 as the proximate factor in the Cnp1/CENP-A deposition pathway, providing a direct connection to centromere-localized Mis16-Mis18. ...

journal_title：Molecular cell

authors： Baker RE

更新日期：2009-02-27 00:00:00

abstract：:The functional significance of mono-, di-, and trimethylation of lysine residues within histone proteins remains unclear. Antibodies developed to selectively recognize each of these methylated states at histone H3 lysine 9 (H3 Lys9) demonstrated that mono- and dimethylation localized specifically to silent domains wit...

journal_title：Molecular cell

authors： Rice JC,Briggs SD,Ueberheide B,Barber CM,Shabanowitz J,Hunt DF,Shinkai Y,Allis CD

更新日期：2003-12-01 00:00:00

abstract：:tRNAs reading four-codon families often have a modified uridine, cmo(5)U(34), at the wobble position of the anticodon. Here, we examine the effects on the decoding mechanism of a cmo(5)U modification in tRNA(1B)(Ala), anticodon C(36)G(35)cmo(5)U(34). tRNA(1B)(Ala) reads its cognate codons in a manner that is very simi...

journal_title：Molecular cell

authors： Kothe U,Rodnina MV

更新日期：2007-01-12 00:00:00

abstract：:The activation of Rab GTPases is a critical focal point of membrane trafficking events in eukaryotic cells; however, the cellular mechanisms that spatially and temporally regulate this process are poorly understood. Here, we identify a null allele of ELP1 as a suppressor of a mutant in a Rab guanine nucleotide exchang...

journal_title：Molecular cell

authors： Rahl PB,Chen CZ,Collins RN

更新日期：2005-03-18 00:00:00

abstract：:BRCA1 and BRCA2 account for most cases of familial, early onset breast and/or ovarian cancer and encode products that each interact with hRAD51. Results presented here show that BRCA1 and BRCA2 coexist in a biochemical complex and colocalize in subnuclear foci in somatic cells and on the axial elements of developing s...

journal_title：Molecular cell

authors： Chen J,Silver DP,Walpita D,Cantor SB,Gazdar AF,Tomlinson G,Couch FJ,Weber BL,Ashley T,Livingston DM,Scully R

更新日期：1998-09-01 00:00:00

abstract：:One of the earliest steps in metazoan pre-mRNA splicing involves binding of U2 snRNP auxiliary factor (U2AF) 65 KDa subunit to the polypyrimidine (Py) tract and of the 35 KDa subunit to the invariant AG dinucleotide at the intron 3' end. Here we use in vitro and in vivo depletion, as well as reconstitution assays usin...

journal_title：Molecular cell

authors： Tavanez JP,Madl T,Kooshapur H,Sattler M,Valcárcel J

更新日期：2012-02-10 00:00:00

abstract：:Whereas most kinesins motor along microtubules, KinI kinesins are microtubule depolymerizing machines. Surprisingly, we found that a KinI fragment consisting of only the motor core is capable of ATP-dependent depolymerization. The motor binds along microtubules in all nucleotide states, but in the presence of AMPPNP, ...

journal_title：Molecular cell

authors： Moores CA,Yu M,Guo J,Beraud C,Sakowicz R,Milligan RA

更新日期：2002-04-01 00:00:00

abstract：:In this issue of Molecular Cell, Vonk et al. (2020) and Thiruvalluvan et al. (2020) identify key chaperones that confer resistance to protein aggregation in neural stem cells and become reduced upon differentiation. ...

journal_title：Molecular cell

authors： Molzahn CM,Mayor T

更新日期：2020-04-16 00:00:00

abstract：:The translocon is the gateway to the endoplasmic reticulum (ER). In yeast this is the Sec61p complex. However, new evidence suggests that a second translocon containing the Sec61p homolog Ssh1p provides important flexibility to the ER translocation machinery. ...

journal_title：Molecular cell

authors： Robb A,Brown JD

更新日期：2001-09-01 00:00:00

abstract：:Nonsense-mediated decay (NMD) degrades mRNAs containing a premature termination codon (PTC). PTCs are a frequent cause of human genetic diseases, and the NMD pathway is known to modulate disease severity. Since partial NMD attenuation can potentially enhance nonsense suppression therapies, better definition of human-s...

journal_title：Molecular cell

authors： Alexandrov A,Shu MD,Steitz JA

更新日期：2017-01-05 00:00:00

abstract：:The 3D structures of all 12 mispairs formed in the active site of a DNA polymerase help explain their differential effects on polymerase stalling and on translocation of the primer terminus to the enzyme's proofreading site. ...

journal_title：Molecular cell

authors： Jiricny J

更新日期：2004-03-26 00:00:00

abstract：:In this issue of Molecular Cell, Lu et al. (2019) analyze the role of the length and sequence complexity of the RNA polymerase II unstructured C-terminal domain in animal viability, development, and the dynamics of RNA polymerase II in vivo. ...

journal_title：Molecular cell

authors： Isaac RS,Churchman LS

更新日期：2019-03-21 00:00:00

abstract：:The stress response in eukaryotic cells often inhibits translation initiation and leads to the formation of cytoplasmic RNA-protein complexes referred to as stress granules. Stress granules contain nontranslating mRNAs, translation initiation components, and many additional proteins affecting mRNA function. Stress gra...

journal_title：Molecular cell

authors： Buchan JR,Parker R

更新日期：2009-12-25 00:00:00

abstract：:In this issue of Molecular Cell, Hogues et al. (2008) demonstrate a wholesale shift in the key regulatory protein involved in ribosomal protein (RP) synthesis during the evolution of S. cerevisiae and, en passant, raise interesting questions about the relationship between RP genes and telomeres. ...

journal_title：Molecular cell

authors： Bhattacharya A,Warner JR

更新日期：2008-03-14 00:00:00

abstract：:Hydroxyl radical footprinting and directed probing from Fe(II)-derivatized IF3 have been used to map the interaction of IF3 relative to 16S rRNA and tRNA(Met)(f) in the 30S ribosomal subunit. Our results place the two domains of IF3 on opposite sides of the initiator tRNA, with the C domain at the platform interface a...

journal_title：Molecular cell

authors： Dallas A,Noller HF

更新日期：2001-10-01 00:00:00

abstract：:We have analyzed two residues in the helicase domain of the E1 initiator protein. These residues are part of a highly conserved structural motif, the beta-hairpin, which is present in the helicase domain of all papovavirus initiator proteins. These proteins are unique in their ability to transition from local template...

journal_title：Molecular cell

authors： Liu X,Schuck S,Stenlund A

更新日期：2007-03-23 00:00:00

abstract：:Bursts of nascent mRNA have been shown to lead to substantial cell-cell variation in unicellular organisms, facilitating diverse responses to environmental challenges. It is unknown whether similar bursts and gene-expression noise occur in mammalian tissues. To address this, we combine single molecule transcript count...

journal_title：Molecular cell

authors： Bahar Halpern K,Tanami S,Landen S,Chapal M,Szlak L,Hutzler A,Nizhberg A,Itzkovitz S

更新日期：2015-04-02 00:00:00

abstract：:Polyubiquitination is a posttranslational modification where ubiquitin chains containing isopeptide bonds linking one of seven ubiquitin lysines with the C terminus of an adjoining ubiquitin are covalently attached to proteins. While functions of K48- and K63-linked polyubiquitin are understood, the role(s) of noncano...

journal_title：Molecular cell

authors： Matsumoto ML,Wickliffe KE,Dong KC,Yu C,Bosanac I,Bustos D,Phu L,Kirkpatrick DS,Hymowitz SG,Rape M,Kelley RF,Dixit VM

更新日期：2010-08-13 00:00:00

abstract：:To date, cross-species comparisons of genetic interactomes have been restricted to small or functionally related gene sets, limiting our ability to infer evolutionary trends. To facilitate a more comprehensive analysis, we constructed a genome-scale epistasis map (E-MAP) for the fission yeast Schizosaccharomyces pombe...

journal_title：Molecular cell

authors： Ryan CJ,Roguev A,Patrick K,Xu J,Jahari H,Tong Z,Beltrao P,Shales M,Qu H,Collins SR,Kliegman JI,Jiang L,Kuo D,Tosti E,Kim HS,Edelmann W,Keogh MC,Greene D,Tang C,Cunningham P,Shokat KM,Cagney G,Svensson JP,Gut

更新日期：2012-06-08 00:00:00

abstract：:Structures of intact receptors with single-pass transmembrane domains are essential to understand how extracellular and cytoplasmic domains regulate association and signaling through transmembrane domains. A chemical and computational method to determine structures of the membrane regions of such receptors on the cell...

journal_title：Molecular cell

authors： Zhu J,Luo BH,Barth P,Schonbrun J,Baker D,Springer TA

更新日期：2009-04-24 00:00:00

abstract：:Heparan sulfate proteoglycans (HSPGs) influence the signaling of many growth factors, and recent work shows that they can promote VEGF signaling in trans from the surface of an adjacent cell. This mode of signaling also alters the activation state and internalization of VEGF receptors. ...

journal_title：Molecular cell

authors： Selleck SB

更新日期：2006-05-19 00:00:00