Abstract:
:There are few tools available for dissecting and elucidating the functions of DNA satellites and other nongenic DNA. To address this, we have explored the experimental potential of DNA sequence-specific drugs containing pyrrole and imidazole amino acids (polyamides). Compounds were synthesized that target different Drosophila melanogaster satellites. Dimeric oligopyrroles were shown to target the AT-rich satellites I, III, and SARs (scaffold associated regions). One polyamide (P31) specifically binds the GAGAA satellite V. Specificity of targeting was established by footprinting, epifluorescence of nuclei, and polytene chromosomes stained with fluorescent derivatives. These polyamides were shown to mediate satellite-specific chromatin opening of the chromatin fiber. Remarkably, certain polyamides induced defined gain or loss-of-function phenotypes when fed to Drosophila melanogaster.
journal_name
Mol Celljournal_title
Molecular cellauthors
Janssen S,Durussel T,Laemmli UKdoi
10.1016/s1097-2765(00)00099-xsubject
Has Abstractpub_date
2000-11-01 00:00:00pages
999-1011issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(00)00099-Xjournal_volume
6pub_type
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