Frozen in Transcription: Cryo-EM Structures of Pol II Transcribing through a Nucleosome.

abstract：:To unravel the roles of LXRs in inflammation and immunity, we examined the function of LXRs in development of IFN-gamma-mediated inflammation using cultured rat brain astrocytes. LXR ligands inhibit neither STAT1 phosphorylation nor STAT1 translocation to the nucleus but, rather, inhibit STAT1 binding to promoters and...

journal_title：Molecular cell

authors： Lee JH,Park SM,Kim OS,Lee CS,Woo JH,Park SJ,Joe EH,Jou I

更新日期：2009-09-24 00:00:00

abstract：:We demonstrate that the major in vivo targets of brefeldin A (BFA) in the secretory pathway of budding yeast are the three members of the Sec7 domain family of ARF exchange factors: Gea1p and Gea2p (functionally interchangeable) and Sec7p. Specific residues within the Sec7 domain are important for BFA inhibition of AR...

journal_title：Molecular cell

authors： Peyroche A,Antonny B,Robineau S,Acker J,Cherfils J,Jackson CL

更新日期：1999-03-01 00:00:00

abstract：:The crystal structure of a heterodimer between the ligand-binding domains (LBDs) of the human RARalpha bound to a selective antagonist and the constitutively active mouse RXRalphaF318A mutant shows that, pushed by a bulky extension of the ligand, RARalpha helix H12 adopts an antagonist position. The unexpected presenc...

journal_title：Molecular cell

authors： Bourguet W,Vivat V,Wurtz JM,Chambon P,Gronemeyer H,Moras D

更新日期：2000-02-01 00:00:00

abstract：:Budding yeast Cdc13, Stn1, and Ten1 form the CST complex to protect telomeres from lethal DNA degradation. It remains unknown whether similar complexes are conserved in higher eukaryotes or not. Here we isolated mammalian STN1 and TEN1 homologs and CTC1 (conserved telomere maintenance component 1). The three proteins ...

journal_title：Molecular cell

authors： Miyake Y,Nakamura M,Nabetani A,Shimamura S,Tamura M,Yonehara S,Saito M,Ishikawa F

更新日期：2009-10-23 00:00:00

abstract：:The exosome plays key roles in RNA maturation and surveillance, but it is unclear how target RNAs are identified. We report the functional characterization of the yeast exosome component Rrp44, a member of the RNase II family. Recombinant Rrp44 and the purified TRAMP polyadenylation complex each specifically recognize...

journal_title：Molecular cell

authors： Schneider C,Anderson JT,Tollervey D

更新日期：2007-07-20 00:00:00

abstract：:Nucleosomes are barriers to transcription in vitro; however, their effects on RNA polymerase in vivo are unknown. Here we describe a simple and general strategy to comprehensively map the positions of elongating and arrested RNA polymerase II (RNAPII) at nucleotide resolution. We find that the entry site of the first ...

journal_title：Molecular cell

authors： Weber CM,Ramachandran S,Henikoff S

更新日期：2014-03-06 00:00:00

abstract：:In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied ini...

journal_title：Molecular cell

authors： Duchi D,Bauer DL,Fernandez L,Evans G,Robb N,Hwang LC,Gryte K,Tomescu A,Zawadzki P,Morichaud Z,Brodolin K,Kapanidis AN

更新日期：2016-09-15 00:00:00

abstract：:The identification of metazoan origins of DNA replication has so far been hampered by the lack of a suitable genetic screening and by the cumbersomeness of the currently available mapping procedures. Here we describe the construction of a library of nascent DNA, representative of all cellular origin sequences, and its...

journal_title：Molecular cell

authors： Todorovic V,Giadrossi S,Pelizon C,Mendoza-Maldonado R,Masai H,Giacca M

更新日期：2005-08-19 00:00:00

abstract：:How repeating striped patterns arise across cellular fields is unclear. To address this we examined the repeating pattern of Stripe (Sr) expression across the parasegment (PS) in Drosophila. This pattern is generated in two steps. First, the ligands Hedgehog (Hh) and Wingless (Wg) subdivide the PS into smaller territo...

journal_title：Molecular cell

authors： Hatini V,DiNardo S

更新日期：2001-01-01 00:00:00

abstract：:Nonsense-mediated decay (NMD) is a surveillance system that degrades mRNAs containing a premature termination codon (PTC) and plays important roles in protein homeostasis and disease. The efficiency of NMD is variable, impacting the clinical outcome of genetic mutations. However, limited resolution of bulk analyses ha...

journal_title：Molecular cell

authors： Hoek TA,Khuperkar D,Lindeboom RGH,Sonneveld S,Verhagen BMP,Boersma S,Vermeulen M,Tanenbaum ME

更新日期：2019-07-25 00:00:00

abstract：:In Drosophila, specification of embryonic terminal cells is controlled by the Torso receptor tyrosine kinase. Here, we analyze the molecular basis of positive (Y630) and negative (Y918) phosphotyrosine (pY) signaling sites on Torso. We find that the Drosophila homolog of RasGAP associates with pY918 and is a negative ...

journal_title：Molecular cell

authors： Cleghon V,Feldmann P,Ghiglione C,Copeland TD,Perrimon N,Hughes DA,Morrison DK

更新日期：1998-12-01 00:00:00

abstract：:It has been suspected that cell-cycle progression might be functionally coupled with RNA processing. However, little is known about the role of the precise splicing control in cell-cycle progression. Here, we report that SON, a large Ser/Arg (SR)-related protein, is a splicing cofactor contributing to efficient splici...

journal_title：Molecular cell

authors： Ahn EY,DeKelver RC,Lo MC,Nguyen TA,Matsuura S,Boyapati A,Pandit S,Fu XD,Zhang DE

更新日期：2011-04-22 00:00:00

abstract：:Alternative splicing (AS) is a widespread process underlying the generation of transcriptomic and proteomic diversity and is frequently misregulated in human disease. Accordingly, an important goal of biomedical research is the development of tools capable of comprehensively, accurately, and efficiently profiling AS. ...

journal_title：Molecular cell

authors： Sterne-Weiler T,Weatheritt RJ,Best AJ,Ha KCH,Blencowe BJ

更新日期：2018-10-04 00:00:00

abstract：:The Rictor/mTOR complex (also known as mTORC2) plays a critical role in cellular homeostasis by phosphorylating AGC kinases such as Akt and SGK at their hydrophobic motifs to activate downstream signaling. However, the regulation of mTORC2 and whether it has additional function(s) remain largely unknown. Here, we repo...

journal_title：Molecular cell

authors： Gao D,Wan L,Inuzuka H,Berg AH,Tseng A,Zhai B,Shaik S,Bennett E,Tron AE,Gasser JA,Lau A,Gygi SP,Harper JW,DeCaprio JA,Toker A,Wei W

更新日期：2010-09-10 00:00:00

abstract：:In this issue of Molecular Cell, Huang et al. (2009) describe two heterotrimeric single-stranded DNA binding complexes, SOSS1 and SOSS2, that function downstream of the MRN complex to promote DNA repair and the G2/M checkpoint. ...

journal_title：Molecular cell

authors： Nam EA,Cortez D

更新日期：2009-08-14 00:00:00

abstract：:Distinctive from their normal counterparts, cancer cells exhibit unique metabolic dependencies on glutamine to fuel anabolic processes. Specifically, pancreatic ductal adenocarcinoma (PDAC) cells rely on an unconventional metabolic pathway catalyzed by aspartate aminotransferase, malate dehydrogenase 1 (MDH1), and mal...

journal_title：Molecular cell

authors： Wang YP,Zhou W,Wang J,Huang X,Zuo Y,Wang TS,Gao X,Xu YY,Zou SW,Liu YB,Cheng JK,Lei QY

更新日期：2016-11-17 00:00:00

abstract：:Recent work from Kuehner and Brow (2008) and Thiebaut et al. (2008) in Molecular Cell and Jenks et al. (2008) in Molecular and Cellular Biology reveals that regulated expression of central nucleotide synthesis pathway components directs start site-dependent RNA polymerase II termination. ...

journal_title：Molecular cell

authors： Dichtl B

更新日期：2008-09-05 00:00:00

abstract：:In eukaryotes, a crucial step of translation initiation is the binding of the multifactor complex eIF4F to the 5' end of the mRNA, a prerequisite to recruitment of the activated small ribosomal 43S particle. Histone H4 mRNAs have short 5'UTRs, which do not conform to the conventional scanning-initiation model. Here we...

journal_title：Molecular cell

authors： Martin F,Barends S,Jaeger S,Schaeffer L,Prongidi-Fix L,Eriani G

更新日期：2011-01-21 00:00:00

abstract：:To determine whether replicational mutagenesis in the yeast genome is influenced by the positions of active origins, a reporter gene was placed in two orientations at multiple locations within a 39,000 bp region of chromosome III possessing two strong origins. The frequency of mutations resulting from misincorporation...

journal_title：Molecular cell

authors： Pavlov YI,Newlon CS,Kunkel TA

更新日期：2002-07-01 00:00:00

abstract：:Prions constitute a rare class of protein, which can switch to a robust amyloid form and then propagate that form in the absence of a nucleic acid determinant, thereby creating a unique, protein-only infectious agent. Details of the mechanism that drives conversion to the prion form and then subsequent propagation of ...

journal_title：Molecular cell

authors： Tuite MF,Koloteva-Levin N

更新日期：2004-06-04 00:00:00

abstract：:The eukaryotic RNA polymerases Pol I, II, and III use different promoters to transcribe different classes of genes. Promoter usage relies on initiation factors, including TFIIF and TFIIE, in the case of Pol II. Here, we show that the Pol I-specific subunits A49 and A34.5 form a subcomplex that binds DNA and is related...

journal_title：Molecular cell

authors： Geiger SR,Lorenzen K,Schreieck A,Hanecker P,Kostrewa D,Heck AJ,Cramer P

更新日期：2010-08-27 00:00:00

abstract：:Using improved techniques, Baptista et al. (2017) and Warfield et al. (2017) revisit fundamental questions about SAGA and TFIID function in yeast. They conclude that each complex independently contributes to the expression of all genes transcribed by RNA polymerase II. ...

journal_title：Molecular cell

authors： Taatjes DJ

更新日期：2017-10-05 00:00:00

abstract：:Transcription is repressed if a DNA double-strand break (DSB) is introduced in close proximity to a transcriptional activation site at least in part by H2A-ubiquitination. While ATM signaling is involved, how it controls H2A-ubiquitination remains unclear. Here, we identify that, in response to DSBs, a transcriptional...

journal_title：Molecular cell

authors： Ui A,Nagaura Y,Yasui A

更新日期：2015-05-07 00:00:00

abstract：:Homologous recombination (HR) is crucial for genetic exchange and accurate repair of DNA double-strand breaks and is pivotal for genome integrity. HR uses homologous sequences for repair, but how homology search, the exploration of the genome for homologous DNA sequences, is conducted in the nucleus remains poorly und...

journal_title：Molecular cell

authors： Renkawitz J,Lademann CA,Kalocsay M,Jentsch S

更新日期：2013-04-25 00:00:00

abstract：:Metazoan histone mRNAs end in a highly conserved stem-loop structure followed by ACCCA. Previous studies have suggested that the stem-loop binding protein (SLBP) is the only protein binding this region. Using RNA affinity purification, we identified a second protein, designated 3'hExo, that contains a SAP and a 3' exo...

journal_title：Molecular cell

authors： Dominski Z,Yang XC,Kaygun H,Dadlez M,Marzluff WF

更新日期：2003-08-01 00:00:00

abstract：:Trans-sialidases (TS) are GPI-anchored surface enzymes expressed in specific developmental stages of trypanosome parasites like Trypanosoma cruzi, the etiologic agent of Chagas disease, and T. brucei, the causative agent of sleeping sickness. TS catalyzes the transfer of sialic acid residues from host to parasite glyc...

journal_title：Molecular cell

authors： Buschiazzo A,Amaya MF,Cremona ML,Frasch AC,Alzari PM

更新日期：2002-10-01 00:00:00

abstract：:Protein function originates from a cooperation of structural rigidity, dynamics at different timescales, and allostery. However, how these three pillars of protein function are integrated is still only poorly understood. Here we show how these pillars are connected in Protein Tyrosine Phosphatase 1B (PTP1B), a drug ta...

journal_title：Molecular cell

authors： Choy MS,Li Y,Machado LESF,Kunze MBA,Connors CR,Wei X,Lindorff-Larsen K,Page R,Peti W

更新日期：2017-02-16 00:00:00

abstract：:Zinc is an essential trace element, and impaired zinc homeostasis is implicated in the pathogenesis of various human diseases. However, the mechanisms cells use to respond to zinc deficiency are poorly understood. We previously reported that amyotrophic lateral sclerosis (ALS)-linked pathogenic mutants of SOD1 cause c...

journal_title：Molecular cell

authors： Homma K,Fujisawa T,Tsuburaya N,Yamaguchi N,Kadowaki H,Takeda K,Nishitoh H,Matsuzawa A,Naguro I,Ichijo H

更新日期：2013-10-10 00:00:00

abstract：:Human cleavage factor I(m) (CFI(m)) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFI(m) can stimulate both cleavage and poly(A) addition, and can act to suppress poly(A) site cleavage in a sequence-depe...

journal_title：Molecular cell

authors： Brown KM,Gilmartin GM

更新日期：2003-12-01 00:00:00

abstract：:Thousands of cis-elements in genomes are predicted to have vital functions. Although conservation, activity in surrogate assays, polymorphisms, and disease mutations provide functional clues, deletion from endogenous loci constitutes the gold-standard test. A GATA-2-binding, Gata2 intronic cis-element (+9.5) required ...

journal_title：Molecular cell

authors： Hewitt KJ,Kim DH,Devadas P,Prathibha R,Zuo C,Sanalkumar R,Johnson KD,Kang YA,Kim JS,Dewey CN,Keles S,Bresnick EH

更新日期：2015-07-02 00:00:00