Abstract:
:The Rictor/mTOR complex (also known as mTORC2) plays a critical role in cellular homeostasis by phosphorylating AGC kinases such as Akt and SGK at their hydrophobic motifs to activate downstream signaling. However, the regulation of mTORC2 and whether it has additional function(s) remain largely unknown. Here, we report that Rictor associates with Cullin-1 to form a functional E3 ubiquitin ligase. Rictor, but not Raptor or mTOR alone, promotes SGK1 ubiquitination. Loss of Rictor/Cullin-1-mediated ubiquitination leads to increased SGK1 protein levels as detected in Rictor null cells. Moreover, as part of a feedback mechanism, phosphorylation of Rictor at T1135 by multiple AGC kinases disrupts the interaction between Rictor and Cullin-1 to impair SGK1 ubiquitination. These findings indicate that the Rictor/Cullin-1 E3 ligase activity is regulated by a specific signal relay cascade and that misregulation of this mechanism may contribute to the frequent overexpression of SGK1 in various human cancers.
journal_name
Mol Celljournal_title
Molecular cellauthors
Gao D,Wan L,Inuzuka H,Berg AH,Tseng A,Zhai B,Shaik S,Bennett E,Tron AE,Gasser JA,Lau A,Gygi SP,Harper JW,DeCaprio JA,Toker A,Wei Wdoi
10.1016/j.molcel.2010.08.016subject
Has Abstractpub_date
2010-09-10 00:00:00pages
797-808issue
5eissn
1097-2765issn
1097-4164pii
S1097-2765(10)00626-Xjournal_volume
39pub_type
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