Allosteric activation of E2-RING finger-mediated ubiquitylation by a structurally defined specific E2-binding region of gp78.

abstract：:Argonaute proteins are required for the biogenesis of some small RNAs (sRNAs), including the PIWI-interacting RNAs and some microRNAs. How Argonautes mediate maturation of sRNAs independent of their slicer activity is not clear. The maturation of the Neurospora microRNA-like sRNA, milR-1, requires the Argonaute protei...

journal_title：Molecular cell

authors： Xue Z,Yuan H,Guo J,Liu Y

更新日期：2012-05-11 00:00:00

abstract：:The ribosome, a ribonucleoprotein machine for protein synthesis, can also serve as an abundant nutrient source under starvation conditions. In a recent issue of Science, Wyant et al. (2018) discovered a specialized "spoon" to "scoop up" more ribosomes for degradation by autophagy. ...

journal_title：Molecular cell

authors： Nakatogawa H

更新日期：2018-07-19 00:00:00

abstract：:Recent studies, including two in this issue of Molecular Cell (Elbatsh et al., 2016; Beckouët et al., 2016), cast light on how cohesin regulators harness the energy of ATP hydrolysis to open the cohesin ring and enable dynamic, regulated entrapment of chromosomes. ...

journal_title：Molecular cell

authors： Yu H

更新日期：2016-02-18 00:00:00

abstract：:Rho factor in bacteria terminates transcription by using energy from ATP hydrolysis to forcefully dissociate the transcripts from RNA polymerase. used data from presteady-state ATPase kinetics to support a rational mechanistic model for Rho's action on RNA. ...

journal_title：Molecular cell

authors： Richardson JP

更新日期：2006-06-23 00:00:00

abstract：:Two cyclin-dependent kinases have been identified in yeast and mammalian RNA polymerase II transcription initiation complexes. We find that the two yeast kinases are indistinguishable in their ability to phosphorylate the RNA polymerase II CTD, and yet in living cells one kinase is a positive regulator and the other a...

journal_title：Molecular cell

authors： Hengartner CJ,Myer VE,Liao SM,Wilson CJ,Koh SS,Young RA

更新日期：1998-07-01 00:00:00

abstract：:Glycogen is the major mammalian glucose storage cache and is critical for energy homeostasis. Glycogen synthesis in neurons must be tightly controlled due to neuronal sensitivity to perturbations in glycogen metabolism. Lafora disease (LD) is a fatal, congenital, neurodegenerative epilepsy. Mutations in the gene encod...

journal_title：Molecular cell

authors： Raththagala M,Brewer MK,Parker MW,Sherwood AR,Wong BK,Hsu S,Bridges TM,Paasch BC,Hellman LM,Husodo S,Meekins DA,Taylor AO,Turner BD,Auger KD,Dukhande VV,Chakravarthy S,Sanz P,Woods VL Jr,Li S,Vander Kooi CW,Gentry

更新日期：2015-01-22 00:00:00

abstract：:MicroRNAs (miRNAs) inhibit mRNA expression in general by base pairing to the 3'UTR of target mRNAs and consequently inhibiting translation and/or initiating poly(A) tail deadenylation and mRNA destabilization. Here we examine the mechanism and kinetics of miRNA-mediated deadenylation in mouse Krebs-2 ascites extract. ...

journal_title：Molecular cell

authors： Fabian MR,Mathonnet G,Sundermeier T,Mathys H,Zipprich JT,Svitkin YV,Rivas F,Jinek M,Wohlschlegel J,Doudna JA,Chen CY,Shyu AB,Yates JR 3rd,Hannon GJ,Filipowicz W,Duchaine TF,Sonenberg N

更新日期：2009-09-24 00:00:00

abstract：:Cohesion between sister chromatids is mediated by the chromosomal cohesin complex. In budding yeast, cohesin is loaded onto chromosomes during the G1 phase of the cell cycle. During S phase, the replication fork-associated acetyltransferase Eco1 acetylates the cohesin subunit Smc3 to promote the establishment of siste...

journal_title：Molecular cell

authors： Borges V,Lehane C,Lopez-Serra L,Flynn H,Skehel M,Rolef Ben-Shahar T,Uhlmann F

更新日期：2010-09-10 00:00:00

abstract：:In the Neurospora circadian system, the transcription factors White Collar-1 (WC-1) and White Collar-2 (WC-2) activate expression of frq, whose gene product inhibits its own expression. The WC proteins are thought to form an obligate complex; however, chromatin immunoprecipitation (ChIP) indicates that WC-2 binds to t...

journal_title：Molecular cell

authors： Belden WJ,Loros JJ,Dunlap JC

更新日期：2007-02-23 00:00:00

abstract：:Downstream core promoter elements are an expanding class of regulatory sequences that add considerable diversity to the promoter architecture of RNA polymerase II-transcribed genes. We set out to determine the factors necessary for downstream promoter element (DPE)-dependent transcription and find that, against expect...

journal_title：Molecular cell

authors： Lewis BA,Sims RJ 3rd,Lane WS,Reinberg D

更新日期：2005-05-13 00:00:00

abstract：:SOCS3 is essential for regulating the extent, duration, and specificity of cellular responses to cytokines such as G-CSF and IL-6. Here we describe the solution structure of SOCS3, the first structure determined for any SOCS protein, in complex with a phosphotyrosine-containing peptide from the IL-6 receptor signaling...

journal_title：Molecular cell

authors： Babon JJ,McManus EJ,Yao S,DeSouza DP,Mielke LA,Sprigg NS,Willson TA,Hilton DJ,Nicola NA,Baca M,Nicholson SE,Norton RS

更新日期：2006-04-21 00:00:00

abstract：:Heterochromatin is refractory to DNA transactions, including repair. In a recent issue of Molecular Cell, Goodarzi et al. (2008) reveal how the central transducing kinase of the DNA damage response relieves this natural barrier by increasing heterochromatic DNA accessibility. ...

journal_title：Molecular cell

authors： Fernandez-Capetillo O,Nussenzweig A

更新日期：2008-08-08 00:00:00

abstract：:Telomeres protect chromosome ends from fusing to double-stranded breaks (DSBs). Using a quantitative real-time PCR assay, we show that nonhomologous end joining between a telomere and an inducible DSB was undetectable in wild-type cells, but occurred within a few hours of DSB induction in approximately 1/2000 genomes ...

journal_title：Molecular cell

authors： Chan SW,Blackburn EH

更新日期：2003-05-01 00:00:00

abstract：:Interference with nucleocytoplasmic transport is a strategy employed by certain viruses to compromise host cellular function. While it has been shown that the matrix (M) protein of the vesicular stomatitis virus (VSV) inhibits nuclear export of host cell mRNAs, the underlying mechanism has not been fully established. ...

journal_title：Molecular cell

authors： Faria PA,Chakraborty P,Levay A,Barber GN,Ezelle HJ,Enninga J,Arana C,van Deursen J,Fontoura BM

更新日期：2005-01-07 00:00:00

abstract：:Argonaute proteins associate with small RNAs that guide mRNA degradation, translational repression, or a combination of both. The human Argonaute family has eight members, four of which (Ago1 through Ago4) are closely related and coexpressed in many cell types. To understand the biological function of the different Ag...

journal_title：Molecular cell

authors： Meister G,Landthaler M,Patkaniowska A,Dorsett Y,Teng G,Tuschl T

更新日期：2004-07-23 00:00:00

abstract：:Siz1 is a founding member of the Siz/PIAS RING family of SUMO E3 ligases. The X-ray structure of an active Siz1 ligase revealed an elongated tripartite architecture comprised of an N-terminal PINIT domain, a central zinc-containing RING-like SP-RING domain, and a C-terminal domain we term the SP-CTD. Structure-based m...

journal_title：Molecular cell

authors： Yunus AA,Lima CD

更新日期：2009-09-11 00:00:00

abstract：:The C-terminal fragment, Bb, of factor B combines with C3b to form the pivotal C3-convertase, C3bBb, of alternative complement pathway. Bb consists of a von Willebrand factor type A (vWFA) domain that is structurally similar to the I domains of integrins and a serine protease (SP) domain that is in inactive conformati...

journal_title：Molecular cell

authors： Ponnuraj K,Xu Y,Macon K,Moore D,Volanakis JE,Narayana SV

更新日期：2004-04-09 00:00:00

abstract：:Activation of NF-kappaB is regulated via numerous ubiquitin- and proteasome-mediated steps; an important one is processing of the precursor p105 to the p50 active subunit. The mechanisms involved are largely unknown, because this is an exceptional case where the ubiquitin system does not destroy its substrate complete...

journal_title：Molecular cell

authors： Kravtsova-Ivantsiv Y,Cohen S,Ciechanover A

更新日期：2009-02-27 00:00:00

abstract：:Kong et al. (2020) present the low-resolution structure of the ATPɣS-bound human condensin I and II complexes and demonstrate that human condensins can extrude DNA loops in a symmetric and asymmetric fashion and compact nucleosome-bound DNA. ...

journal_title：Molecular cell

authors： Goloborodko A

更新日期：2020-07-02 00:00:00

abstract：:In addition to its role as an electron transporter, mitochondrial nicotinamide adenine dinucleotide (NAD+) is an important co-factor for enzymatic reactions, including ADP-ribosylation. Although mitochondria harbor the most intra-cellular NAD+, mitochondrial ADP-ribosylation remains poorly understood. Here we provide ...

journal_title：Molecular cell

authors： Hopp AK,Teloni F,Bisceglie L,Gondrand C,Raith F,Nowak K,Muskalla L,Howald A,Pedrioli PGA,Johnsson K,Altmeyer M,Pedrioli DML,Hottiger MO

更新日期：2021-01-21 00:00:00

abstract：:B and T cell receptor gene assembly by V(D)J recombination is tightly regulated during lymphoid development. The mechanisms involved in this regulation are poorly understood. Here we show that nucleosomal DNA is refractory to V(D)J cleavage. However, the presence of HMG1, a chromatin-associated nonhistone DNA-binding ...

journal_title：Molecular cell

authors： Kwon J,Imbalzano AN,Matthews A,Oettinger MA

更新日期：1998-12-01 00:00:00

abstract：:The IkappaB kinases IKKalpha and IKKbeta regulate distinct cytoplasmic and nuclear events that are critical for cytokine-mediated activation of the NF-kappaB pathway. Because the IKKs have previously been demonstrated to associate with the nuclear hormone receptor coactivator AIB1/SRC-3, the question of whether either...

journal_title：Molecular cell

authors： Park KJ,Krishnan V,O'Malley BW,Yamamoto Y,Gaynor RB

更新日期：2005-04-01 00:00:00

abstract：:In this issue of Molecular Cell, detail molecular events that trigger apoptosis following growth factor withdrawal, linking irreversible mitochondrial permeabilization to activation of GSK-3 and subsequent phosphorylation, ubiquitinylation, and degradation of the antiapoptotic BCL-2 family member MCL-1. ...

journal_title：Molecular cell

authors： Letai A

更新日期：2006-03-17 00:00:00

abstract：:Chromatin governs gene regulation and genome maintenance, yet a substantial fraction of the chromatin proteome is still unexplored. Moreover, a global model of the chromatin protein network is lacking. By screening >100 candidates we identify 42 Drosophila proteins that were not previously associated with chromatin, w...

journal_title：Molecular cell

authors： van Bemmel JG,Filion GJ,Rosado A,Talhout W,de Haas M,van Welsem T,van Leeuwen F,van Steensel B

更新日期：2013-02-21 00:00:00

abstract：:Cryo-EM structures of Dot1L in complex with a ubiquitinated nucleosome provide the long-sought-after molecular mechanism of Dot1L-mediated methylation of lysine 79 in histone H3 and explain crosstalk with histone H2B ubiquitination. ...

journal_title：Molecular cell

authors： Zhang Y,Kutateladze TG

更新日期：2019-06-06 00:00:00

abstract：:Bicoid (BCD), the anterior determinant of Drosophila, controls embryonic gene expression by transcriptional activation and translational repression. Both functions require the homeodomain (HD), which recognizes DNA motifs at target gene enhancers and a specific sequence interval in the 3' untranslated region of caudal...

journal_title：Molecular cell

authors： Niessing D,Driever W,Sprenger F,Taubert H,Jäckle H,Rivera-Pomar R

更新日期：2000-02-01 00:00:00

abstract：:RNA binding proteins (RBPs) regulate all aspects in the life cycle of RNA molecules. To elucidate the elements that guide RNA specificity, regulatory mechanisms, and functions of RBPs, methods that identify direct endogenous protein-RNA interactions are particularly valuable. UV crosslinking and immunoprecipitation (C...

journal_title：Molecular cell

authors： Lee FCY,Ule J

更新日期：2018-02-01 00:00:00

abstract：:The splitting of chromosomes in anaphase and their delivery into the daughter cells needs to be accurately executed to maintain genome stability. Chromosome splitting requires the degradation of securin, whereas the distribution of the chromosomes into the daughter cells requires the degradation of cyclin B. We show t...

journal_title：Molecular cell

authors： Kamenz J,Mihaljev T,Kubis A,Legewie S,Hauf S

更新日期：2015-11-05 00:00:00

abstract：:Both splicing factors and microRNAs are important regulatory molecules that play key roles in posttranscriptional gene regulation. By miRNA deep sequencing, we identified 40 miRNAs that are differentially expressed upon ectopic overexpression of the splicing factor SF2/ASF. Here we show that SF2/ASF and one of its upr...

journal_title：Molecular cell

authors： Wu H,Sun S,Tu K,Gao Y,Xie B,Krainer AR,Zhu J

更新日期：2010-04-09 00:00:00

abstract：:The positive transcription elongation factor b (P-TEFb) is a cyclin-dependent kinase that controls the elongation phase of transcription by RNA polymerase II (RNAPII). This process is made possible by the reversal of effects of negative elongation factors that include NELF and DSIF. In complex organisms, elongation co...

journal_title：Molecular cell

authors： Peterlin BM,Price DH

更新日期：2006-08-04 00:00:00