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Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.

Abstract：

:Argonaute proteins associate with small RNAs that guide mRNA degradation, translational repression, or a combination of both. The human Argonaute family has eight members, four of which (Ago1 through Ago4) are closely related and coexpressed in many cell types. To understand the biological function of the different Ago proteins, we set out to determine if Ago1 through Ago4 are associated with miRNAs as well as RISC activity in human cell lines. Our results suggest that miRNAs are incorporated indiscriminately of their sequence into Ago1 through Ago4 containing microRNPs (miRNPs). Purification of the FLAG/HA-epitope-tagged Ago containing complexes from different human cell lines revealed that endonuclease activity is exclusively associated with Ago2. Exogenously introduced siRNAs also associate with Ago2 for guiding target RNA cleavage. The specific role of Ago2 in guiding target RNA cleavage was confirmed independently by siRNA-based depletion of individual Ago members in combination with a sensitive positive-readout reporter assay.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Meister G,Landthaler M,Patkaniowska A,Dorsett Y,Teng G,Tuschl T

doi

10.1016/j.molcel.2004.07.007

subject

Has Abstract

pub_date

2004-07-23 00:00:00

pages

185-97

issue

2

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(04)00415-0

journal_volume

15

pub_type

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