PARP-1 Flips the Epigenetic Switch on Obesity.

abstract：:Zinc is an essential trace element, and impaired zinc homeostasis is implicated in the pathogenesis of various human diseases. However, the mechanisms cells use to respond to zinc deficiency are poorly understood. We previously reported that amyotrophic lateral sclerosis (ALS)-linked pathogenic mutants of SOD1 cause c...

journal_title：Molecular cell

authors： Homma K,Fujisawa T,Tsuburaya N,Yamaguchi N,Kadowaki H,Takeda K,Nishitoh H,Matsuzawa A,Naguro I,Ichijo H

更新日期：2013-10-10 00:00:00

abstract：:Posttranslational modification of proliferating cell nuclear antigen (PCNA), an essential processivity clamp for DNA polymerases, by ubiquitin and SUMO contributes to the coordination of DNA replication, damage tolerance, and mutagenesis. Whereas ubiquitination in response to DNA damage promotes the bypass of replicat...

journal_title：Molecular cell

authors： Papouli E,Chen S,Davies AA,Huttner D,Krejci L,Sung P,Ulrich HD

更新日期：2005-07-01 00:00:00

abstract：:Innate immune responses are critical for the immediate protection against microbial infection. In Drosophila, infection leads to the rapid and robust production of antimicrobial peptides through two NF-kappaB signaling pathways-IMD and Toll. The IMD pathway is triggered by DAP-type peptidoglycan, common to most Gram-n...

journal_title：Molecular cell

authors： Paquette N,Broemer M,Aggarwal K,Chen L,Husson M,Ertürk-Hasdemir D,Reichhart JM,Meier P,Silverman N

更新日期：2010-01-29 00:00:00

abstract：:The Fanconi anemia (FA) pathway is implicated in DNA repair and cancer predisposition. Central to this pathway is the FA core complex, which is targeted to chromatin by FANCM and FAAP24 following replication stress. Here we show that FANCM and FAAP24 interact with the checkpoint protein HCLK2 independently of the FA c...

journal_title：Molecular cell

authors： Collis SJ,Ciccia A,Deans AJ,Horejsí Z,Martin JS,Maslen SL,Skehel JM,Elledge SJ,West SC,Boulton SJ

更新日期：2008-11-07 00:00:00

abstract：:Regulatory sequences or erroneous incorporations during DNA transcription cause RNA polymerase backtracking and inactivation in all kingdoms of life. Reactivation requires RNA transcript cleavage. Essential transcription factors (GreA and GreB, or TFIIS) accelerate this reaction. We report four cryo-EM reconstructions...

journal_title：Molecular cell

authors： Abdelkareem M,Saint-André C,Takacs M,Papai G,Crucifix C,Guo X,Ortiz J,Weixlbaumer A

更新日期：2019-07-25 00:00:00

abstract：:Cas9 is a prokaryotic RNA-guided DNA endonuclease that binds substrates tightly in vitro but turns over rapidly when used to manipulate genomes in eukaryotic cells. Little is known about the factors responsible for dislodging Cas9 or how they influence genome engineering. Unbiased detection through proximity labeling ...

journal_title：Molecular cell

authors： Wang AS,Chen LC,Wu RA,Hao Y,McSwiggen DT,Heckert AB,Richardson CD,Gowen BG,Kazane KR,Vu JT,Wyman SK,Shin JJ,Darzacq X,Walter JC,Corn JE

更新日期：2020-07-16 00:00:00

abstract：:In solid tissues of vertebrates, initiation of mitochondrial DNA replication encompasses a broad zone downstream of the major noncoding region (NCR). In contrast, analysis with two-dimensional agarose gel electrophoresis of mitochondrial DNA replication intermediates in cultured human cells revealed initiation concent...

journal_title：Molecular cell

authors： Yasukawa T,Yang MY,Jacobs HT,Holt IJ

更新日期：2005-06-10 00:00:00

abstract：:The selective degradation of intracellular components by autophagy involves sequential interactions of the cargo with a receptor, which also binds the autophagosomal protein Atg8 and a scaffold protein. Here, we demonstrated that mutations in C. elegans epg-11, which encodes an arginine methyltransferase homologous to...

journal_title：Molecular cell

authors： Li S,Yang P,Tian E,Zhang H

更新日期：2013-11-07 00:00:00

abstract：:During hematopoiesis, cells originating from the same stem cell reservoir differentiate into distinct cell types. The mechanisms enabling common progenitors to differentiate into alternative cell fates are not fully understood. Here, we identify cell-fate-determining transcription factors (TFs) governing dendritic cel...

journal_title：Molecular cell

authors： Bornstein C,Winter D,Barnett-Itzhaki Z,David E,Kadri S,Garber M,Amit I

更新日期：2014-12-18 00:00:00

abstract：:Transcription is a source of genetic instability that can notably result from the formation of genotoxic DNA:RNA hybrids, or R-loops, between the nascent mRNA and its template. Here we report an unexpected function for introns in counteracting R-loop accumulation in eukaryotic genomes. Deletion of endogenous introns i...

journal_title：Molecular cell

authors： Bonnet A,Grosso AR,Elkaoutari A,Coleno E,Presle A,Sridhara SC,Janbon G,Géli V,de Almeida SF,Palancade B

更新日期：2017-08-17 00:00:00

abstract：:Although DNA looping between the initiator binding sites (iterons) of the replication origin (ori) of a plasmid and the iterons located in a cis-acting control sequence called inc has been postulated to promote negative control of plasmid DNA replication, not only was definitive evidence for such looping lacking, but ...

journal_title：Molecular cell

authors： Zzaman S,Bastia D

更新日期：2005-12-22 00:00:00

abstract：:While gapmers efficiently knock down as well as terminate transcription of nascent lncRNAs and mRNAs, Lee and Mendell (2020) and Lai et al. (2020) also demonstrate that Pol II termination is not observed with gapmers targeting the 3' terminal portions of the transcript. ...

journal_title：Molecular cell

authors： Maranon DG,Wilusz J

更新日期：2020-03-05 00:00:00

abstract：:A decline in mitochondrial quality and activity has been associated with normal aging and correlated with the development of a wide range of age-related diseases. Here, we review the evidence that a decline in mitochondria function contributes to aging. In particular, we discuss how mitochondria contribute to specific...

journal_title：Molecular cell

authors： Sun N,Youle RJ,Finkel T

更新日期：2016-03-03 00:00:00

abstract：:RNA editing in the mitochondria of kinetoplastids involves the addition and deletion of uridines at specific sites as directed by guide RNAs (gRNAs). Ample evidence shows that ribonucleoprotein (RNP) complexes carry out this posttranscriptional processing. One component of RNA editing complexes is REAP-1, a protein of...

journal_title：Molecular cell

authors： Madison-Antenucci S,Hajduk SL

更新日期：2001-04-01 00:00:00

abstract：:Microtubule plus end binding proteins (+TIPs) localize to the dynamic plus ends of microtubules, where they stimulate microtubule growth and recruit signaling molecules. Three main +TIP classes have been identified (XMAP215, EB1, and CLIP-170), but whether they act upon microtubule plus ends through a similar mechanis...

journal_title：Molecular cell

authors： Slep KC,Vale RD

更新日期：2007-09-21 00:00:00

abstract：:The early detection by the host of invading microorganisms, including viruses, depends on a limited number of specific receptors that recognize pathogen-associated molecular patterns (PAMPs). A few of these PAMPs, including ssRNA and dsRNA, are recognized by Toll-like receptors (TLR)-7/8 and TLR3, respectively. Activa...

journal_title：Molecular cell

authors： Meylan E,Tschopp J

更新日期：2006-06-09 00:00:00

abstract：:The intracellular regulation of cell death pathways by cIAPs has been enigmatic. Here we show that loss of cIAPs promotes the spontaneous formation of an intracellular platform that activates either apoptosis or necroptosis. This 2 MDa intracellular complex that we designate "Ripoptosome" is necessary but not sufficie...

journal_title：Molecular cell

authors： Feoktistova M,Geserick P,Kellert B,Dimitrova DP,Langlais C,Hupe M,Cain K,MacFarlane M,Häcker G,Leverkus M

更新日期：2011-08-05 00:00:00

abstract：:Our previous study showed that ubiquitination of p53 is reversible and that the ubiquitin hydrolase HAUSP can stabilize p53 by deubiquitination. Here, we found that partial reduction of endogenous HAUSP levels by RNAi indeed destabilizes endogenous p53; surprisingly, however, nearly complete ablation of HAUSP stabiliz...

journal_title：Molecular cell

authors： Li M,Brooks CL,Kon N,Gu W

更新日期：2004-03-26 00:00:00

abstract：:In Saccharomyces cerevisiae, methylation of histone H3 at active genes is an epigenetic mark that distinguishes active from silent chromatin and functions as a short-term "memory" of recent transcription. Methylation of H3 at lysine residues K4 and K79 depends on ubiquitylation of histone H2B, but the mechanisms linki...

journal_title：Molecular cell

authors： Ezhkova E,Tansey WP

更新日期：2004-02-13 00:00:00

abstract：:Mammalian target of rapamycin (mTOR) activity is regulated by assembly of two functionally distinct complexes, mTORC1 and mTORC2. In syndecan-4 (S4) null endothelial cells, mTORC2 activity is reduced, resulting in decreased Akt activation, while mTORC1 activity is increased. Levels of rictor, mLST8, and mSin-1 are unc...

journal_title：Molecular cell

authors： Partovian C,Ju R,Zhuang ZW,Martin KA,Simons M

更新日期：2008-10-10 00:00:00

abstract：:Prenylated Rab proteins exist in the cytosol as soluble, high-affinity complexes with GDI that need to be disrupted for membrane attachment and targeting of Rab proteins. The Legionella pneumophila protein DrrA displaces GDI from Rab1:GDI complexes, incorporating Rab1 into Legionella-containing vacuoles and activating...

journal_title：Molecular cell

authors： Schoebel S,Oesterlin LK,Blankenfeldt W,Goody RS,Itzen A

更新日期：2009-12-25 00:00:00

abstract：:Networks of protein interactions coordinate cellular functions. We describe a bimolecular fluorescence complementation (BiFC) assay for determination of the locations of protein interactions in living cells. This approach is based on complementation between two nonfluorescent fragments of the yellow fluorescent protei...

journal_title：Molecular cell

authors： Hu CD,Chinenov Y,Kerppola TK

更新日期：2002-04-01 00:00:00

abstract：:In budding yeast, the nuclear RNA surveillance system is active on all pre-mRNA transcripts and modulated by nutrient availability. To test the role of nuclear surveillance in reprogramming gene expression, we identified transcriptome-wide binding sites for RNA polymerase II and the exosome cofactors Mtr4 (TRAMP compl...

journal_title：Molecular cell

authors： Bresson S,Tuck A,Staneva D,Tollervey D

更新日期：2017-03-02 00:00:00

abstract：:In response to hyperosmotic environments, most eukaryotic cells activate a specialized mitogen-activated protein (MAP) kinase pathway. In S. cerevisiae, the key protein kinase, Hog1, coordinates the transcriptional induction of a variety of genes devoted to osmoadaptation and general stress protection. Depending on th...

journal_title：Molecular cell

authors： Alepuz PM,Jovanovic A,Reiser V,Ammerer G

更新日期：2001-04-01 00:00:00

abstract：:The splitting of chromosomes in anaphase and their delivery into the daughter cells needs to be accurately executed to maintain genome stability. Chromosome splitting requires the degradation of securin, whereas the distribution of the chromosomes into the daughter cells requires the degradation of cyclin B. We show t...

journal_title：Molecular cell

authors： Kamenz J,Mihaljev T,Kubis A,Legewie S,Hauf S

更新日期：2015-11-05 00:00:00

abstract：:The heterodimeric nuclear cap binding complex (CBC) binds to 5'-capped polymerase II transcripts. It enhances the efficiency of several mRNA maturation steps and is essential for U snRNA nuclear export in multicellular eukaryotes. The 2A crystal structure of human CBC shows that the large subunit, CBP80, comprises thr...

journal_title：Molecular cell

authors： Mazza C,Ohno M,Segref A,Mattaj IW,Cusack S

更新日期：2001-08-01 00:00:00

abstract：:Protein function originates from a cooperation of structural rigidity, dynamics at different timescales, and allostery. However, how these three pillars of protein function are integrated is still only poorly understood. Here we show how these pillars are connected in Protein Tyrosine Phosphatase 1B (PTP1B), a drug ta...

journal_title：Molecular cell

authors： Choy MS,Li Y,Machado LESF,Kunze MBA,Connors CR,Wei X,Lindorff-Larsen K,Page R,Peti W

更新日期：2017-02-16 00:00:00

abstract：:Infections can cause a multitude of stresses on the host and microbe. To detect potential infections, the mammalian immune system utilizes several families of pattern recognition receptors, which survey the intracellular and extracellular environments for microbial products. Members of each receptor family induce anti...

journal_title：Molecular cell

authors： Franz KM,Kagan JC

更新日期：2017-06-15 00:00:00

abstract：:The translocon is the gateway to the endoplasmic reticulum (ER). In yeast this is the Sec61p complex. However, new evidence suggests that a second translocon containing the Sec61p homolog Ssh1p provides important flexibility to the ER translocation machinery. ...

journal_title：Molecular cell

authors： Robb A,Brown JD

更新日期：2001-09-01 00:00:00

abstract：:Recent studies, including two in this issue of Molecular Cell (Elbatsh et al., 2016; Beckouët et al., 2016), cast light on how cohesin regulators harness the energy of ATP hydrolysis to open the cohesin ring and enable dynamic, regulated entrapment of chromosomes. ...

journal_title：Molecular cell

authors： Yu H

更新日期：2016-02-18 00:00:00