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The Histone Chaperone FACT Induces Cas9 Multi-turnover Behavior and Modifies Genome Manipulation in Human Cells.

Abstract：

:Cas9 is a prokaryotic RNA-guided DNA endonuclease that binds substrates tightly in vitro but turns over rapidly when used to manipulate genomes in eukaryotic cells. Little is known about the factors responsible for dislodging Cas9 or how they influence genome engineering. Unbiased detection through proximity labeling of transient protein interactions in cell-free Xenopus laevis egg extract identified the dimeric histone chaperone facilitates chromatin transcription (FACT) as an interactor of substrate-bound Cas9. FACT is both necessary and sufficient to displace dCas9, and FACT immunodepletion converts Cas9's activity from multi-turnover to single turnover. In human cells, FACT depletion extends dCas9 residence times, delays genome editing, and alters the balance between indel formation and homology-directed repair. FACT knockdown also increases epigenetic marking by dCas9-based transcriptional effectors with a concomitant enhancement of transcriptional modulation. FACT thus shapes the intrinsic cellular response to Cas9-based genome manipulation most likely by determining Cas9 residence times.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Wang AS,Chen LC,Wu RA,Hao Y,McSwiggen DT,Heckert AB,Richardson CD,Gowen BG,Kazane KR,Vu JT,Wyman SK,Shin JJ,Darzacq X,Walter JC,Corn JE

doi

10.1016/j.molcel.2020.06.014

subject

Has Abstract

pub_date

2020-07-16 00:00:00

pages

221-233.e5

issue

2

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(20)30399-3

journal_volume

79

pub_type

杂志文章

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