Abstract:
:Ten E. coli aminoacyl-tRNAs (aa-tRNAs) were assessed for their ability to decode cognate codons on E. coli ribosomes by using three assays that evaluate the key steps in the decoding pathway. Despite a wide variety of structural features, each aa-tRNA exhibited similar kinetic and thermodynamic properties in each assay. This surprising kinetic and thermodynamic uniformity is likely to reflect the importance of ribosome conformational changes in defining the rates and affinities of the decoding process as well as the evolutionary "tuning" of each aa-tRNA sequence to modify their individual interactions with the ribosome at each step.
journal_name
Mol Celljournal_title
Molecular cellauthors
Ledoux S,Uhlenbeck OCdoi
10.1016/j.molcel.2008.04.026subject
Has Abstractpub_date
2008-07-11 00:00:00pages
114-23issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(08)00335-3journal_volume
31pub_type
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