Spoon-Feeding Ribosomes to Autophagy.

abstract：:The papillomavirus major late protein, L1, forms the pentameric assembly unit of the viral shell. Recombinant HPV16 L1 pentamers assemble in vitro into capsid-like structures, and truncation of ten N-terminal residues leads to a homogeneous preparation of 12-pentamer, icosahedral particles. X-ray crystallographic anal...

journal_title：Molecular cell

authors： Chen XS,Garcea RL,Goldberg I,Casini G,Harrison SC

更新日期：2000-03-01 00:00:00

abstract：:Nuclear import of proteins containing a classical nuclear localization signal (NLS) involves NLS recognition by importin alpha, which associates with importin beta via the IBB domain. Other proteins, including parathyroid hormone-related protein (PTHrP), are imported into the nucleus by direct interaction with importi...

journal_title：Molecular cell

authors： Cingolani G,Bednenko J,Gillespie MT,Gerace L

更新日期：2002-12-01 00:00:00

abstract：:Cse4 is a variant of histone H3 that is incorporated into a single nucleosome at each centromere in budding yeast. We have discovered an E3 ubiquitin ligase, called Psh1, which controls the cellular level of Cse4 via ubiquitylation and proteolysis. The activity of Psh1 is dependent on both its RING and zinc finger dom...

journal_title：Molecular cell

authors： Hewawasam G,Shivaraju M,Mattingly M,Venkatesh S,Martin-Brown S,Florens L,Workman JL,Gerton JL

更新日期：2010-11-12 00:00:00

abstract：:Alternative lengthening of telomeres (ALT) is a homology-directed repair (HDR) mechanism of telomere elongation that controls proliferation in aggressive cancers. We show that the disruption of RAD51-associated protein 1 (RAD51AP1) in ALT+ cancer cells leads to generational telomere shortening. This is due to RAD51AP1...

journal_title：Molecular cell

authors： Barroso-González J,García-Expósito L,Hoang SM,Lynskey ML,Roncaioli JL,Ghosh A,Wallace CT,de Vitis M,Modesti M,Bernstein KA,Sarkar SN,Watkins SC,O'Sullivan RJ

更新日期：2019-10-03 00:00:00

abstract：:In this issue, Song et al. (2014) describe how microtubules and the RAN GTPase/importin-β system collaborate to control timing of HURP action and its turnover via the anaphase promoting complex (APC)-proteasome system, thereby ensuring temporal control of late mitotic events. ...

journal_title：Molecular cell

authors： Vaites LL,Harper JW

更新日期：2014-01-23 00:00:00

abstract：:Metazoan spliceosomes exhibit an elaborate protein composition required for canonical and alternative splicing. Thus, the minimal set of proteins essential for activation and catalysis remains elusive. We therefore purified in vitro assembled, precatalytic spliceosomal complex B, activated B(act), and step 1 complex C...

journal_title：Molecular cell

authors： Fabrizio P,Dannenberg J,Dube P,Kastner B,Stark H,Urlaub H,Lührmann R

更新日期：2009-11-25 00:00:00

abstract：:In this issue of Molecular Cell, Pont et al. (2012) show that AUF1/hnRNP D promotes TERT transcription, which is required for telomere maintenance in mice. ...

journal_title：Molecular cell

authors： Chen LY,Lingner J

更新日期：2012-07-13 00:00:00

abstract：:Spt6 is an essential histone chaperone that mediates nucleosome reassembly during gene transcription. Spt6 also associates with RNA polymerase II (RNAPII) via a tandem Src2 homology domain. However, the significance of Spt6-RNAPII interaction is not well understood. Here, we show that Spt6 recruitment to genes and the...

journal_title：Molecular cell

authors： Dronamraju R,Hepperla AJ,Shibata Y,Adams AT,Magnuson T,Davis IJ,Strahl BD

更新日期：2018-06-21 00:00:00

abstract：:The Mre11-Rad50-Xrs2 (MRX) complex is related to SMC complexes that form rings capable of holding two distinct DNA strands together. MRX functions at stalled replication forks and double-strand breaks (DSBs). A mutation in the N-terminal OB fold of the 70 kDa subunit of yeast replication protein A, rfa1-t11, abrogates...

journal_title：Molecular cell

authors： Seeber A,Hegnauer AM,Hustedt N,Deshpande I,Poli J,Eglinger J,Pasero P,Gut H,Shinohara M,Hopfner KP,Shimada K,Gasser SM

更新日期：2016-12-01 00:00:00

abstract：:Cardiac disease remains the leading cause of morbidity and mortality worldwide. The β1-adrenergic receptor (β1-AR) is a major regulator of cardiac functions and is downregulated in the majority of heart failure cases. A key physiological process is the activation of heterotrimeric G-protein Gs by β1-ARs, leading to in...

journal_title：Molecular cell

authors： Su M,Zhu L,Zhang Y,Paknejad N,Dey R,Huang J,Lee MY,Williams D,Jordan KD,Eng ET,Ernst OP,Meyerson JR,Hite RK,Walz T,Liu W,Huang XY

更新日期：2020-10-01 00:00:00

abstract：:The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II) comprises multiple heptapeptide repeats of the consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Reversible phosphorylation of Ser2, Ser5, and Ser7 during the transcription cycle mediates the sequential recruitment of transcription/RNA pr...

journal_title：Molecular cell

authors： Egloff S,Zaborowska J,Laitem C,Kiss T,Murphy S

更新日期：2012-01-13 00:00:00

abstract：:For single-cell organisms, nutrient uptake and metabolism are central to the fundamental decision of whether to grow or divide. In metazoans, cell fate decisions are more complex: organismal homeostasis must be strictly maintained by balancing cell proliferation and death. Despite this increased complexity, cell fate ...

journal_title：Molecular cell

authors： Andersen JL,Kornbluth S

更新日期：2013-02-07 00:00:00

abstract：:Ribosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering poorly understood large-scale structural transitions that we analyzed by cryo-electron microscopy. Two nuclear pre-60S inte...

journal_title：Molecular cell

authors： Kater L,Mitterer V,Thoms M,Cheng J,Berninghausen O,Beckmann R,Hurt E

更新日期：2020-08-20 00:00:00

abstract：:Androgen receptor (AR) plays a critical role in the development and progression of prostate cancer, where it is a key therapeutic target. Here we report that, in contrast to estrogen receptor transcription complexes which form within minutes and recycle hourly, the levels of regulatory regions bound by AR complexes ri...

journal_title：Molecular cell

authors： Wang Q,Carroll JS,Brown M

更新日期：2005-09-02 00:00:00

abstract：:The Wnt3a/β-catenin and Activin/SMAD2,3 signaling pathways synergize to induce endodermal differentiation of human embryonic stem cells; however, the underlying mechanism is not well understood. Using ChIP-seq and GRO-seq analyses, we show here that Wnt3a-induced β-catenin:LEF-1 enhancers recruit cohesin to direct enh...

journal_title：Molecular cell

authors： Estarás C,Benner C,Jones KA

更新日期：2015-06-04 00:00:00

abstract：:The Raf/MEK/ERK and PI3K/Akt pathways are prominent effectors of oncogenic Ras. These pathways negatively regulate each other, but the mechanism involved is incompletely understood. We now identify MEK1 as an essential regulator of lipid/protein phosphatase PTEN, through which it controls phosphatidylinositol-3-phosph...

journal_title：Molecular cell

authors： Zmajkovicova K,Jesenberger V,Catalanotti F,Baumgartner C,Reyes G,Baccarini M

更新日期：2013-04-11 00:00:00

abstract：:Brr2 is a unique DExD/H box protein required for catalytic activation and disassembly of the spliceosome. It contains two tandem helicase cassettes that both comprise dual RecA-like domains and a noncanonical Sec63 unit. The latter may bestow the enzyme with unique properties. We have determined crystal structures of ...

journal_title：Molecular cell

authors： Pena V,Jovin SM,Fabrizio P,Orlowski J,Bujnicki JM,Lührmann R,Wahl MC

更新日期：2009-08-28 00:00:00

abstract：:We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-beta-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, inc...

journal_title：Molecular cell

authors： Navarre WW,Zou SB,Roy H,Xie JL,Savchenko A,Singer A,Edvokimova E,Prost LR,Kumar R,Ibba M,Fang FC

更新日期：2010-07-30 00:00:00

abstract：:Two publications in the current issue of Cell introduce novel methods for high-throughput single-cell transcriptomics by using droplet microfluidics and sophisticated barcoding schemes for transcriptional profiling of thousands of individual cells. ...

journal_title：Molecular cell

authors： Junker JP,van Oudenaarden A

更新日期：2015-05-21 00:00:00

abstract：:In Drosophila, specification of embryonic terminal cells is controlled by the Torso receptor tyrosine kinase. Here, we analyze the molecular basis of positive (Y630) and negative (Y918) phosphotyrosine (pY) signaling sites on Torso. We find that the Drosophila homolog of RasGAP associates with pY918 and is a negative ...

journal_title：Molecular cell

authors： Cleghon V,Feldmann P,Ghiglione C,Copeland TD,Perrimon N,Hughes DA,Morrison DK

更新日期：1998-12-01 00:00:00

abstract：:N6-methyladenosine (m6A), the most abundant internal mRNA modification, and N6,2'-O-dimethyladenosine (m6Am), found at the first-transcribed nucleotide, are two reversible epitranscriptomic marks. However, the profiles and distribution patterns of m6A and m6Am across human and mouse tissues are poorly characterized. H...

journal_title：Molecular cell

authors： Liu J,Li K,Cai J,Zhang M,Zhang X,Xiong X,Meng H,Xu X,Huang Z,Peng J,Fan J,Yi C

更新日期：2020-01-16 00:00:00

abstract：:Many genomic studies have revealed associations between the gut microbiota composition and host metabolism. These observations led to the idea that a causal relationship could exist between the microbiota and metabolic diseases, a concept supported by studies showing compositional changes in the microbial community in...

journal_title：Molecular cell

authors： Koh A,Bäckhed F

更新日期：2020-05-21 00:00:00

abstract：:Tumor suppressor genes evolved as negative effectors of mitogen and nutrient signaling pathways, such that mutations in these genes can lead to pathological states of growth. Tuberous sclerosis (TSC) is a potentially devastating disease associated with mutations in two tumor suppressor genes, TSC1 and 2, that function...

journal_title：Molecular cell

authors： Garami A,Zwartkruis FJ,Nobukuni T,Joaquin M,Roccio M,Stocker H,Kozma SC,Hafen E,Bos JL,Thomas G

更新日期：2003-06-01 00:00:00

abstract：:p73 has been identified as a structural and functional homolog of the tumor suppressor p53. The transcriptional coactivator Yes-associated protein (YAP) has been demonstrated to interact with and to enhance p73-dependent apoptosis in response to DNA damage. Here, we show the existence of a proapoptotic autoregulatory ...

journal_title：Molecular cell

authors： Lapi E,Di Agostino S,Donzelli S,Gal H,Domany E,Rechavi G,Pandolfi PP,Givol D,Strano S,Lu X,Blandino G

更新日期：2008-12-26 00:00:00

abstract：:We have developed a highly parallel strategy, systematic gene-to-phenotype arrays (SGPAs), to comprehensively map the genetic landscape driving molecular phenotypes of interest. By this approach, a complete yeast genetic mutant array is crossed with fluorescent reporters and imaged on membranes at high density and con...

journal_title：Molecular cell

authors： Jaeger PA,Ornelas L,McElfresh C,Wong LR,Hampton RY,Ideker T

更新日期：2018-01-18 00:00:00

abstract：:Plus-end tracking proteins, such as EB1 and the dynein/dynactin complex, regulate microtubule dynamics. These proteins are thought to stabilize microtubules by forming a plus-end complex at microtubule growing ends with ill-defined mechanisms. Here we report the crystal structure of two plus-end complex components, th...

journal_title：Molecular cell

authors： Hayashi I,Wilde A,Mal TK,Ikura M

更新日期：2005-08-19 00:00:00

abstract：:The Drosophila gene vasa (vas) encodes an RNA-binding protein required for embryonic patterning and germ cell specification. In vas mutants, translation of several germline mRNAs is reduced. Here we show that VAS interacts directly with the Drosophila homolog of yeast translation initiation factor 2, encoded by a nove...

journal_title：Molecular cell

authors： Carrera P,Johnstone O,Nakamura A,Casanova J,Jäckle H,Lasko P

更新日期：2000-01-01 00:00:00

abstract：:The autophagy cargo receptor p62 facilitates the condensation of misfolded, ubiquitin-positive proteins and their degradation by autophagy, but the molecular mechanism of p62 signaling to the core autophagy machinery is unclear. Here, we show that disordered residues 326-380 of p62 directly interact with the C-termina...

journal_title：Molecular cell

authors： Turco E,Witt M,Abert C,Bock-Bierbaum T,Su MY,Trapannone R,Sztacho M,Danieli A,Shi X,Zaffagnini G,Gamper A,Schuschnig M,Fracchiolla D,Bernklau D,Romanov J,Hartl M,Hurley JH,Daumke O,Martens S

更新日期：2019-04-18 00:00:00

abstract：:CRISPR-Cas12a (Cpf1) is an RNA-guided DNA-cutting nuclease that has been repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target DNA in cis and non-target single-stranded DNAs (ssDNAs) in trans. To elucidate the molecular basis for both DNase cleavage modes, we performed structural and b...

journal_title：Molecular cell

authors： Swarts DC,Jinek M

更新日期：2019-02-07 00:00:00

abstract：:Heparan sulfate proteoglycans (HSPGs) influence the signaling of many growth factors, and recent work shows that they can promote VEGF signaling in trans from the surface of an adjacent cell. This mode of signaling also alters the activation state and internalization of VEGF receptors. ...

journal_title：Molecular cell

authors： Selleck SB

更新日期：2006-05-19 00:00:00