Abstract：
:HIV-1 Nef plays important roles in HIV-1 replication and pathogenesis. It is translated from completely spliced HIV-1 RNA, and its expression is inherently regulated at the levels of viral DNA transcription and RNA splicing. Here we show that Sam68 cytoplasmic mutants potently suppress Nef expression. The suppression requires Sam68 domain aa 269-321 and is correlated with its ability to induce stress granules. In addition, the suppression is specific to Nef, and direct binding to nef mRNA 3'UTR confers the suppression specificity. Furthermore, nef mRNA is targeted to and enriched in these induced stress granules. Importantly, Nef suppression occurs in the context of HIV-1 infection of CD4+ T lymphocytes with little MHC I and CD4 downregulation. Taken together, these results demonstrate that stress granule induction and nef mRNA sequestration account for this translational suppression of Nef expression and offer a strategy for development of anti-HIV therapeutics to buttress our fight against HIV/AIDS.
journal_name
Mol Celljournal_title
Molecular cellauthors
Henao-Mejia J,Liu Y,Park IW,Zhang J,Sanford J,He JJdoi
10.1016/j.molcel.2008.11.024subject
Has Abstractpub_date
2009-01-16 00:00:00pages
87-96issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(08)00862-9journal_volume
33pub_type
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