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Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer.

Abstract：

:Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can also control EDI splicing, arguing for coupling between the transcription and splicing machineries. We now report that the SR proteins SF2/ASF and 9G8 stimulate EDI splicing in vivo and that their effect requires an intact EDI ESE. Most importantly, we show that sensitivity to these SR proteins critically depends on the promoter structure, suggesting that the transcription machinery modulates their recruitment to the ESE.

journal_name

Mol Cell

journal_title

Molecular cell

authors

Cramer P,Cáceres JF,Cazalla D,Kadener S,Muro AF,Baralle FE,Kornblihtt AR

doi

10.1016/s1097-2765(00)80372-x

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

251-8

issue

2

eissn

1097-2765

issn

1097-4164

pii

S1097-2765(00)80372-X

journal_volume

4

pub_type

杂志文章

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