Spatial Organization of Single mRNPs at Different Stages of the Gene Expression Pathway.

abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptati...

journal_title：Molecular cell

authors： Sternberg SH,Richter H,Charpentier E,Qimron U

更新日期：2016-03-17 00:00:00

abstract：:The crystal structure of a heterodimer between the ligand-binding domains (LBDs) of the human RARalpha bound to a selective antagonist and the constitutively active mouse RXRalphaF318A mutant shows that, pushed by a bulky extension of the ligand, RARalpha helix H12 adopts an antagonist position. The unexpected presenc...

journal_title：Molecular cell

authors： Bourguet W,Vivat V,Wurtz JM,Chambon P,Gronemeyer H,Moras D

更新日期：2000-02-01 00:00:00

abstract：:MRE11 and RAD50 are known to be required for nonhomologous joining of DNA ends in vivo. We have investigated the enzymatic activities of the purified proteins and found that Mre11 by itself has 3' to 5' exonuclease activity that is increased when Mre11 is in a complex with Rad50. Mre11 also exhibits endonuclease activ...

journal_title：Molecular cell

authors： Paull TT,Gellert M

更新日期：1998-06-01 00:00:00

abstract：:Clathrin-dependent endocytosis has long been presented as the only efficient mechanism by which transmembrane receptors are internalized. We selectively blocked this process using dominant-negative mutants of Eps15 and showed that clathrin-mediated endocytosis of transferrin was inhibited, while endocytosis of interle...

journal_title：Molecular cell

authors： Lamaze C,Dujeancourt A,Baba T,Lo CG,Benmerah A,Dautry-Varsat A

更新日期：2001-03-01 00:00:00

abstract：:The duplication of eukaryotic genomes involves the replication of DNA from multiple origins of replication. In S phase, two sister replisomes assemble at each active origin, and they replicate DNA in opposite directions. Little is known about the functional relationship between sister replisomes. Some data imply that ...

journal_title：Molecular cell

authors： Yardimci H,Loveland AB,Habuchi S,van Oijen AM,Walter JC

更新日期：2010-12-10 00:00:00

abstract：:Ubiquitination typically occurs through the sequential action of three enzymes catalyzing ubiquitin activation (E1), conjugation (E2), and ligation (E3) and regulates diverse eukaryotic cellular processes. Although monoubiquitination commonly confers nondegradative activities, mechanisms underlying its temporal and sp...

journal_title：Molecular cell

authors： Sun L,Fang J

更新日期：2016-06-16 00:00:00

abstract：:In yeast, histone H3/H4 exchange independent of replication is poorly understood. Here, we analyzed the deposition of histone H3 molecules, synthesized during G1, using a high-density microarray histone exchange assay. While we found that H3 exchange in coding regions requires high levels of transcription, promoters e...

journal_title：Molecular cell

authors： Rufiange A,Jacques PE,Bhat W,Robert F,Nourani A

更新日期：2007-08-03 00:00:00

abstract：:The 3D structures of all 12 mispairs formed in the active site of a DNA polymerase help explain their differential effects on polymerase stalling and on translocation of the primer terminus to the enzyme's proofreading site. ...

journal_title：Molecular cell

authors： Jiricny J

更新日期：2004-03-26 00:00:00

abstract：:Using genome-wide approaches, we have elucidated the regulatory circuitry governed by the XBP1 transcription factor, a key effector of the mammalian unfolded protein response (UPR), in skeletal muscle and secretory cells. We identified a core group of genes involved in constitutive maintenance of ER function in all ce...

journal_title：Molecular cell

authors： Acosta-Alvear D,Zhou Y,Blais A,Tsikitis M,Lents NH,Arias C,Lennon CJ,Kluger Y,Dynlacht BD

更新日期：2007-07-06 00:00:00

abstract：:N6-methyladenosine (m6A), the most abundant internal mRNA modification, and N6,2'-O-dimethyladenosine (m6Am), found at the first-transcribed nucleotide, are two reversible epitranscriptomic marks. However, the profiles and distribution patterns of m6A and m6Am across human and mouse tissues are poorly characterized. H...

journal_title：Molecular cell

authors： Liu J,Li K,Cai J,Zhang M,Zhang X,Xiong X,Meng H,Xu X,Huang Z,Peng J,Fan J,Yi C

更新日期：2020-01-16 00:00:00

abstract：:Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engageme...

journal_title：Molecular cell

authors： Diebold-Durand ML,Lee H,Ruiz Avila LB,Noh H,Shin HC,Im H,Bock FP,Bürmann F,Durand A,Basfeld A,Ham S,Basquin J,Oh BH,Gruber S

更新日期：2017-07-20 00:00:00

abstract：:In this study, we used Saccharomyces cerevisiae to identify a biological network that prevents the deleterious effects of endogenous reactive oxygen species. The absence of Tsa1, a key peroxiredoxin, caused increased rates of mutations, chromosomal rearrangements, and recombination. Defects in recombinational DNA doub...

journal_title：Molecular cell

authors： Huang ME,Kolodner RD

更新日期：2005-03-04 00:00:00

abstract：:The ER-mitochondrial junction provides a local calcium signaling domain that is critical for both matching energy production with demand and the control of apoptosis. Here, we visualize ER-mitochondrial contact sites and monitor the localized [Ca(2+)] changes ([Ca(2+)](ER-mt)) using drug-inducible fluorescent interorg...

journal_title：Molecular cell

authors： Csordás G,Várnai P,Golenár T,Roy S,Purkins G,Schneider TG,Balla T,Hajnóczky G

更新日期：2010-07-09 00:00:00

abstract：:The anaphase-promoting complex (APC) is a ubiquitin ligase that promotes the degradation of cell-cycle regulators. Cdh1p is an APC coactivator that directly binds APC substrates. A genetic screen in budding yeast identified residues within Cdh1p critical for its function. Cdh1p proteins containing mutations within the...

journal_title：Molecular cell

authors： Burton JL,Tsakraklides V,Solomon MJ

更新日期：2005-05-27 00:00:00

abstract：:PRDM9 determines the localization of meiotic recombination hotspots, which are associated with histone H3 methylation. It is not known whether PRDM9's methyltransferase activity is required or how some PRDM9 alleles can dominate the distribution of hotspots over other alleles. Diagouraga, Clément, and colleagues (2018...

journal_title：Molecular cell

authors： Kang R,Zelazowski MJ,Cole F

更新日期：2018-03-01 00:00:00

abstract：:The structure of an RNA polymerase II/general transcription factor TFIIF complex was determined by cryo-electron microscopy and single particle analysis. Density due to TFIIF was not concentrated in one area but rather was widely distributed across the surface of the polymerase. The largest subunit of TFIIF interacted...

journal_title：Molecular cell

authors： Chung WH,Craighead JL,Chang WH,Ezeokonkwo C,Bareket-Samish A,Kornberg RD,Asturias FJ

更新日期：2003-10-01 00:00:00

abstract：:Progressive phosphorylation of circadian clock proteins is a hallmark of time-keeping. In this issue of Molecular Cell, Querfurth et al. (2011) demonstrate that phosphorylation of Neurospora FRQ induces a conformational change, which can account for its temporally gated degradation. ...

journal_title：Molecular cell

authors： Menet JS,Rosbash M

更新日期：2011-09-02 00:00:00

abstract：:Salmonella force their way into nonphagocytic host intestinal cells to initiate infection. Uptake is triggered by delivery into the target cell of bacterial effector proteins that stimulate cytoskeletal rearrangements and membrane ruffling. The Salmonella invasion protein A (SipA) effector is an actin binding protein ...

journal_title：Molecular cell

authors： McGhie EJ,Hayward RD,Koronakis V

更新日期：2004-02-27 00:00:00

abstract：:A chemicogenetic screen was performed in budding yeast mutants that have a weakened replication stress response. This identified an inhibitor of target of rapamycin (TOR) complexes 1 and 2 that selectively enhances the sensitivity of sgs1Δ cells to hydroxyurea and camptothecin. More importantly, the inhibitor has stro...

journal_title：Molecular cell

authors： Shimada K,Filipuzzi I,Stahl M,Helliwell SB,Studer C,Hoepfner D,Seeber A,Loewith R,Movva NR,Gasser SM

更新日期：2013-09-26 00:00:00

abstract：:Faithful propagation of specific chromatin states requires re-establishment of epigenetic marks after every cell division. How the original epigenetic signature is inherited after disruption during DNA replication is still poorly understood. Here, we show that the poly(ADP-ribose)-polymerase-1 (PARP1/ARTD1) is implica...

journal_title：Molecular cell

authors： Guetg C,Scheifele F,Rosenthal F,Hottiger MO,Santoro R

更新日期：2012-03-30 00:00:00

abstract：:Cancer cells acquire unlimited proliferative capacity by either re-expressing telomerase or inducing alternative lengthening of telomeres (ALT), which relies on telomere recombination. Here, we show that ALT recombination requires coordinate regulation of the SMX and BTR complexes to ensure the appropriate balance of ...

journal_title：Molecular cell

authors： Panier S,Maric M,Hewitt G,Mason-Osann E,Gali H,Dai A,Labadorf A,Guervilly JH,Ruis P,Segura-Bayona S,Belan O,Marzec P,Gaillard PL,Flynn RL,Boulton SJ

更新日期：2019-10-03 00:00:00

abstract：:The oncoprotein BCL-3 is a nuclear transcription factor that activates NF-kappaB target genes through formation of heterocomplexes with p50 or p52. BCL-3 is phosphorylated in vivo, but specific BCL-3 kinases have not been identified so far. In this report, we show that BCL-3 is a substrate for the protein kinase GSK3 ...

journal_title：Molecular cell

authors： Viatour P,Dejardin E,Warnier M,Lair F,Claudio E,Bureau F,Marine JC,Merville MP,Maurer U,Green D,Piette J,Siebenlist U,Bours V,Chariot A

更新日期：2004-10-08 00:00:00

abstract：:MeCP2 is an essential transcriptional repressor that mediates gene silencing through binding to methylated DNA. Binding specificity has been thought to depend on hydrophobic interactions between cytosine methyl groups and a hydrophobic patch within the methyl-CpG-binding domain (MBD). X-ray analysis of a methylated DN...

journal_title：Molecular cell

authors： Ho KL,McNae IW,Schmiedeberg L,Klose RJ,Bird AP,Walkinshaw MD

更新日期：2008-02-29 00:00:00

abstract：:ClpXP is a protein machine composed of the ClpX ATPase, a member of the Clp/Hsp100 family of remodeling enzymes, and the ClpP peptidase. Here, ClpX and ClpXP are shown to catalyze denaturation of GFP modified with an ssrA degradation tag. ClpX translocates this denatured protein into the proteolytic chamber of ClpP an...

journal_title：Molecular cell

authors： Kim YI,Burton RE,Burton BM,Sauer RT,Baker TA

更新日期：2000-04-01 00:00:00

abstract：:Stress granules are mRNA-protein assemblies formed from nontranslating mRNAs. Stress granules are important in the stress response and may contribute to some degenerative diseases. Here, we describe the stress granule transcriptome of yeast and mammalian cells through RNA-sequencing (RNA-seq) analysis of purified stre...

journal_title：Molecular cell

authors： Khong A,Matheny T,Jain S,Mitchell SF,Wheeler JR,Parker R

更新日期：2017-11-16 00:00:00

abstract：:Immune cell function depends on specific metabolic programs dictated by mitochondria, including nutrient oxidation, macromolecule synthesis, and post-translational modifications. Mitochondrial adaptations have been linked to acute and chronic inflammation, but the metabolic cues and precise mechanisms remain unclear. ...

journal_title：Molecular cell

authors： Chi Z,Chen S,Xu T,Zhen W,Yu W,Jiang D,Guo X,Wang Z,Zhang K,Li M,Zhang J,Fang H,Yang D,Ye Q,Yang X,Lin H,Yang F,Zhang X,Wang D

更新日期：2020-10-01 00:00:00

abstract：:SOCS3 is essential for regulating the extent, duration, and specificity of cellular responses to cytokines such as G-CSF and IL-6. Here we describe the solution structure of SOCS3, the first structure determined for any SOCS protein, in complex with a phosphotyrosine-containing peptide from the IL-6 receptor signaling...

journal_title：Molecular cell

authors： Babon JJ,McManus EJ,Yao S,DeSouza DP,Mielke LA,Sprigg NS,Willson TA,Hilton DJ,Nicola NA,Baca M,Nicholson SE,Norton RS

更新日期：2006-04-21 00:00:00

abstract：:The terminal step in cytokinesis that severs a cell in two-abscission-is poorly understood. In Developmental Cell, Fabbro et al (2005) identify a centrosome protein whose multiple phosphorylations regulate its movement from centrosome to midbody and completion of abscission. ...

journal_title：Molecular cell

authors： Doxsey SJ

更新日期：2005-10-28 00:00:00

abstract：:In Drosophila, specification of embryonic terminal cells is controlled by the Torso receptor tyrosine kinase. Here, we analyze the molecular basis of positive (Y630) and negative (Y918) phosphotyrosine (pY) signaling sites on Torso. We find that the Drosophila homolog of RasGAP associates with pY918 and is a negative ...

journal_title：Molecular cell

authors： Cleghon V,Feldmann P,Ghiglione C,Copeland TD,Perrimon N,Hughes DA,Morrison DK

更新日期：1998-12-01 00:00:00

abstract：:Neurons undertake an amazing journey from the center of the developing mammalian brain to the outer layers of the cerebral cortex. Doublecortin, a component of the microtubule cytoskeleton, is essential in postmitotic neurons and was identified because its mutation disrupts human brain development. Doublecortin stabil...

journal_title：Molecular cell

authors： Moores CA,Perderiset M,Francis F,Chelly J,Houdusse A,Milligan RA

更新日期：2004-06-18 00:00:00