Structure of small virus-like particles assembled from the L1 protein of human papillomavirus 16.

abstract：:Thousands of cis-elements in genomes are predicted to have vital functions. Although conservation, activity in surrogate assays, polymorphisms, and disease mutations provide functional clues, deletion from endogenous loci constitutes the gold-standard test. A GATA-2-binding, Gata2 intronic cis-element (+9.5) required ...

journal_title：Molecular cell

authors： Hewitt KJ,Kim DH,Devadas P,Prathibha R,Zuo C,Sanalkumar R,Johnson KD,Kang YA,Kim JS,Dewey CN,Keles S,Bresnick EH

更新日期：2015-07-02 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptati...

journal_title：Molecular cell

authors： Sternberg SH,Richter H,Charpentier E,Qimron U

更新日期：2016-03-17 00:00:00

abstract：:The Mre11/Rad50/Nbs1 complex initiates double-strand break repair by homologous recombination (HR). Loss of Mre11 or its nuclease activity in mouse cells is known to cause genome aberrations and cellular senescence, although the molecular basis for this phenotype is not clear. To identify the origin of these defects, ...

journal_title：Molecular cell

authors： Hoa NN,Shimizu T,Zhou ZW,Wang ZQ,Deshpande RA,Paull TT,Akter S,Tsuda M,Furuta R,Tsutsui K,Takeda S,Sasanuma H

更新日期：2016-11-03 00:00:00

abstract：:High-resolution Cas9 structures have yet to reveal catalytic conformations due to HNH nuclease domain positioning away from the cleavage site. Nme1Cas9 and Nme2Cas9 are compact nucleases for in vivo genome editing. Here, we report structures of meningococcal Cas9 homologs in complex with sgRNA, dsDNA, or the AcrIIC3 a...

journal_title：Molecular cell

authors： Sun W,Yang J,Cheng Z,Amrani N,Liu C,Wang K,Ibraheim R,Edraki A,Huang X,Wang M,Wang J,Liu L,Sheng G,Yang Y,Lou J,Sontheimer EJ,Wang Y

更新日期：2019-12-19 00:00:00

abstract：:The proapoptotic Bcl-2 protein Bax is predominantly found in the cytosol of nonapoptotic cells and is commonly thought to translocate to mitochondria following an apoptotic stimulus. The current model for Bax activation is that BH3 proteins bind to cytosolic Bax, initiating mitochondrial targeting and outer-membrane p...

journal_title：Molecular cell

authors： Schellenberg B,Wang P,Keeble JA,Rodriguez-Enriquez R,Walker S,Owens TW,Foster F,Tanianis-Hughes J,Brennan K,Streuli CH,Gilmore AP

更新日期：2013-03-07 00:00:00

abstract：:The binding of cytoplasmic proteins, such as talin, to the cytoplasmic domains of integrin adhesion receptors mediates bidirectional signal transduction. Here we report the crystal structure of the principal integrin binding and activating fragment of talin, alone and in complex with fragments of the beta 3 integrin t...

journal_title：Molecular cell

authors： García-Alvarez B,de Pereda JM,Calderwood DA,Ulmer TS,Critchley D,Campbell ID,Ginsberg MH,Liddington RC

更新日期：2003-01-01 00:00:00

abstract：:The G protein-coupled receptor Gpr1 and associated Galpha subunit Gpa2 govern dimorphic transitions in response to extracellular nutrients by signaling coordinately with Ras to activate adenylyl cyclase in the yeast Saccharomyces cerevisiae. Gpa2 forms a protein complex with the kelch Gbeta mimic subunits Gpb1/2, and ...

journal_title：Molecular cell

authors： Harashima T,Anderson S,Yates JR 3rd,Heitman J

更新日期：2006-06-23 00:00:00

abstract：:A recent paper by Gallagher et al. (2020) demonstrates that c-di-GMP controls spore formation in Streptomyces venezuelae through sequestering the sporulation sigma factor σWhiG and presents the crystal structure of a ternary complex between c-di-GMP, σWhiG, and its anti-sigma factor, RsiG. ...

journal_title：Molecular cell

authors： Chou SH,Galperin MY

更新日期：2020-02-06 00:00:00

abstract：:Lysine acetylation of the tumor suppressor protein p53 in response to a wide variety of cellular stress signals is required for its activation as a transcription factor that regulates cell cycle arrest, senescence, or apoptosis. Here, we report that the conserved bromo-domain of the transcriptional coactivator CBP (CR...

journal_title：Molecular cell

authors： Mujtaba S,He Y,Zeng L,Yan S,Plotnikova O,Sachchidanand,Sanchez R,Zeleznik-Le NJ,Ronai Z,Zhou MM

更新日期：2004-01-30 00:00:00

abstract：:Oncogene-induced senescence (OIS) is a tumor-suppressive mechanism typified by stable proliferative arrest, a persistent DNA damage response, and the senescence-associated secretory phenotype (SASP), which helps to maintain the senescent state and triggers bystander senescence in a paracrine fashion. Here, we demonstr...

journal_title：Molecular cell

authors： Chen H,Ruiz PD,McKimpson WM,Novikov L,Kitsis RN,Gamble MJ

更新日期：2015-09-03 00:00:00

abstract：:In this issue of Molecular Cell, Savir and Tlusty (2010) apply signal detection theory to show that homologous recombination machinery is optimally tuned to find homologous DNA sequences within an exceptionally high background of heterologous sequences. ...

journal_title：Molecular cell

authors： Rambo RP,Williams GJ,Tainer JA

更新日期：2010-11-12 00:00:00

abstract：:Loss of endogenous tumor suppression is a critical step on the road to cancer. In a recent paper in Molecular Cell, Wang and colleagues provide evidence that inactivation of the pivotal BRCA1 tumor suppressor disrupts a safeguard gene network that opposes cell proliferation and cell survival. ...

journal_title：Molecular cell

authors： Luo J,Altieri DC

更新日期：2008-10-24 00:00:00

abstract：:A study in this issue of Molecular Cell (Elcheva et al., 2009) shows the inherent instability of the betaTrCP1 mRNA to be caused by microRNA-183 targeting the coding sequence; interestingly, this action is directly opposed by the RNA-binding protein CRD-BP. ...

journal_title：Molecular cell

authors： Nielsen AF,Gloggnitzer J,Martinez J

更新日期：2009-07-31 00:00:00

abstract：:Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engageme...

journal_title：Molecular cell

authors： Diebold-Durand ML,Lee H,Ruiz Avila LB,Noh H,Shin HC,Im H,Bock FP,Bürmann F,Durand A,Basfeld A,Ham S,Basquin J,Oh BH,Gruber S

更新日期：2017-07-20 00:00:00

abstract：:RING ubiquitin ligases (E3) recruit ubiquitin-conjugate enzymes (E2) charged with ubiquitin (Ub) to catalyze ubiquitination. Non-covalent Ub binding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains elusive. Here, we show that backside bound Ub (Ub(B)) enhances both RING-in...

journal_title：Molecular cell

authors： Buetow L,Gabrielsen M,Anthony NG,Dou H,Patel A,Aitkenhead H,Sibbet GJ,Smith BO,Huang DT

更新日期：2015-04-16 00:00:00

abstract：:X chromosome inactivation (XCI) depends on a noncoding sense-antisense transcript pair, Xist and Tsix. At the onset of XCI, Xist RNA accumulates on one of two Xs, coating and silencing the chromosome in cis. The molecular basis for monoallelic Xist upregulation is not known, though evidence predominantly supports a po...

journal_title：Molecular cell

authors： Sun BK,Deaton AM,Lee JT

更新日期：2006-03-03 00:00:00

abstract：:Macroautophagy (autophagy) is a lysosome-dependent degradation process that has been implicated in age-associated diseases. Autophagy is involved in both cell survival and cell death, but little is known about the mechanisms that distinguish its use during these distinct cell fates. Here, we identify the microRNA miR-...

journal_title：Molecular cell

authors： Nelson C,Ambros V,Baehrecke EH

更新日期：2014-11-06 00:00:00

abstract：:The Polycomb repressive complex 2 (PRC2) confers transcriptional repression through histone H3 lysine 27 trimethylation (H3K27me3). Here, we examined how PRC2 is modulated by histone modifications associated with transcriptionally active chromatin. We provide the molecular basis of histone H3 N terminus recognition by...

journal_title：Molecular cell

authors： Schmitges FW,Prusty AB,Faty M,Stützer A,Lingaraju GM,Aiwazian J,Sack R,Hess D,Li L,Zhou S,Bunker RD,Wirth U,Bouwmeester T,Bauer A,Ly-Hartig N,Zhao K,Chan H,Gu J,Gut H,Fischle W,Müller J,Thomä NH

更新日期：2011-05-06 00:00:00

abstract：:Faithful duplication of the genome in S phase followed by its accurate segregation in mitosis is essential to maintain genomic integrity. Recent studies have suggested that proteins involved in DNA transactions are also required for whole-chromosome stability. Here we demonstrate that the MRN (Mre11, Rad50, and Nbs1) ...

journal_title：Molecular cell

authors： Rozier L,Guo Y,Peterson S,Sato M,Baer R,Gautier J,Mao Y

更新日期：2013-03-28 00:00:00

abstract：:tRNAs reading four-codon families often have a modified uridine, cmo(5)U(34), at the wobble position of the anticodon. Here, we examine the effects on the decoding mechanism of a cmo(5)U modification in tRNA(1B)(Ala), anticodon C(36)G(35)cmo(5)U(34). tRNA(1B)(Ala) reads its cognate codons in a manner that is very simi...

journal_title：Molecular cell

authors： Kothe U,Rodnina MV

更新日期：2007-01-12 00:00:00

abstract：:Trimethylation of histone H3 on lysine 4 (H3K4me3) localizes near the 5' region of genes and is tightly associated with active loci. Several proteins, such as CHD1, BPTF, JMJD2A, and the ING tumor suppressor family, directly recognize this lysine methyl mark. However, how H3K4me3 recognition participates in active tra...

journal_title：Molecular cell

authors： Sims RJ 3rd,Millhouse S,Chen CF,Lewis BA,Erdjument-Bromage H,Tempst P,Manley JL,Reinberg D

更新日期：2007-11-30 00:00:00

abstract：:Yeast Sir2 protein regulates chromatin structure and suppresses recombination at the multiple tandem rDNA array. In the current issue of Cell, Kobayashi and colleagues reinvestigate rDNA dynamics in sir2 strains and find that Sir2 is necessary to recruit cohesins to the array, which influences the nature of rDNA recom...

journal_title：Molecular cell

authors： Reid RJ,Rothstein R

更新日期：2004-05-21 00:00:00

abstract：:The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II) comprises multiple heptapeptide repeats of the consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Reversible phosphorylation of Ser2, Ser5, and Ser7 during the transcription cycle mediates the sequential recruitment of transcription/RNA pr...

journal_title：Molecular cell

authors： Egloff S,Zaborowska J,Laitem C,Kiss T,Murphy S

更新日期：2012-01-13 00:00:00

abstract：:Sterol accumulation in membranes blocks the exit of SCAP from the ER, preventing SREBP cleavage and reducing cholesterol synthesis. Sterols act through SCAP's sterol-sensing domain by an obscure mechanism. Here, we show that addition of cholesterol to ER membranes in vitro causes a conformational change in SCAP, detec...

journal_title：Molecular cell

authors： Brown AJ,Sun L,Feramisco JD,Brown MS,Goldstein JL

更新日期：2002-08-01 00:00:00

abstract：:Genome stability depends upon the RecQ helicases, which are conserved from bacteria to man, but little is known about how their myriad activities are regulated. Fission yeast lacking the telomere protein Taz1 (mammalian TRF1/TRF2 ortholog) lose many hallmarks of telomeres, including accurate replication and local prot...

journal_title：Molecular cell

authors： Rog O,Miller KM,Ferreira MG,Cooper JP

更新日期：2009-03-13 00:00:00

abstract：:Stress granules (SGs) are cytoplasmic assemblies of mRNPs stalled in translation initiation. They are induced by various stress conditions, including exposure to the environmental toxin and carcinogen arsenic. While perturbed SG turnover is linked to the pathogenesis of neurodegenerative diseases, the molecular mechan...

journal_title：Molecular cell

authors： Turakhiya A,Meyer SR,Marincola G,Böhm S,Vanselow JT,Schlosser A,Hofmann K,Buchberger A

更新日期：2018-06-07 00:00:00

abstract：:Salmonella force their way into nonphagocytic host intestinal cells to initiate infection. Uptake is triggered by delivery into the target cell of bacterial effector proteins that stimulate cytoskeletal rearrangements and membrane ruffling. The Salmonella invasion protein A (SipA) effector is an actin binding protein ...

journal_title：Molecular cell

authors： McGhie EJ,Hayward RD,Koronakis V

更新日期：2004-02-27 00:00:00

abstract：:In mammals, inactivation of one X chromosome in the female equalizes gene dosages between XX females and XY males. Two noncoding loci, Tsix and Xite, together regulate X chromosome fate by controlling homologous chromosome pairing, counting, and mutually exclusive choice. Following choice, the asymmetry of Xite and Ts...

journal_title：Molecular cell

authors： Donohoe ME,Zhang LF,Xu N,Shi Y,Lee JT

更新日期：2007-01-12 00:00:00

abstract：:AAA+ proteins (ATPases associated with various cellular activities) are oligomeric ATPases that use ATP hydrolysis to remodel their substrates. By similarity with GTPases, a dynamic organization of the nucleotide-binding pockets between ATPase protomers is proposed to regulate functionality. Using the transcription ac...

journal_title：Molecular cell

authors： Joly N,Zhang N,Buck M

更新日期：2012-08-10 00:00:00

abstract：:Protein function originates from a cooperation of structural rigidity, dynamics at different timescales, and allostery. However, how these three pillars of protein function are integrated is still only poorly understood. Here we show how these pillars are connected in Protein Tyrosine Phosphatase 1B (PTP1B), a drug ta...

journal_title：Molecular cell

authors： Choy MS,Li Y,Machado LESF,Kunze MBA,Connors CR,Wei X,Lindorff-Larsen K,Page R,Peti W

更新日期：2017-02-16 00:00:00