Abstract：
:The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II) comprises multiple heptapeptide repeats of the consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Reversible phosphorylation of Ser2, Ser5, and Ser7 during the transcription cycle mediates the sequential recruitment of transcription/RNA processing factors. Phosphorylation of Ser7 is required for recruitment of the gene type-specific Integrator complex to the Pol II-transcribed small nuclear (sn)RNA genes. Here, we show that RNA Pol II-associated protein 2 (RPAP2) specifically recognizes the phospho-Ser7 mark on the Pol II CTD and also interacts with Integrator subunits. siRNA-mediated knockdown of RPAP2 and mutation of Ser7 to alanine cause similar defects in snRNA gene expression. In addition, we show that RPAP2 is a CTD Ser5 phosphatase. Taken together, our results indicate that during transcription of snRNA genes, Ser7 phosphorylation facilitates recruitment of RPAP2, which in turn both recruits Integrator and dephosphorylates Ser5.
journal_name
Mol Celljournal_title
Molecular cellauthors
Egloff S,Zaborowska J,Laitem C,Kiss T,Murphy Sdoi
10.1016/j.molcel.2011.11.006subject
Has Abstractpub_date
2012-01-13 00:00:00pages
111-22issue
1eissn
1097-2765issn
1097-4164pii
S1097-2765(11)00856-2journal_volume
45pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract：:The structural diversity of glycans on cells-the glycome-is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates. We used maps of g...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.05.017
更新日期：2019-07-25 00:00:00
abstract：:Recent work from Kuehner and Brow (2008) and Thiebaut et al. (2008) in Molecular Cell and Jenks et al. (2008) in Molecular and Cellular Biology reveals that regulated expression of central nucleotide synthesis pathway components directs start site-dependent RNA polymerase II termination. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2008.08.014
更新日期：2008-09-05 00:00:00
abstract：:mRNAs form ribonucleoprotein complexes (mRNPs) by association with proteins that are crucial for mRNA metabolism. While the mRNP proteome has been well characterized, little is known about mRNP organization. Using a single-molecule approach, we show that mRNA conformation changes depending on its cellular localization...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.10.010
更新日期：2018-11-15 00:00:00
abstract：:Growth factors such as epidermal growth factor (EGF) and insulin regulate development and metabolism via genes containing both POU homeodomain (Pit-1) and phorbol ester (AP-1) response elements. Although CREB binding protein (CBP) functions as a coactivator on these elements, the mechanism of transactivation was previ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(01)00202-7
更新日期：2001-03-01 00:00:00
abstract：:Corticotropin-releasing factor (CRF) and the three related peptides urocortins 1-3 (UCN1-UCN3) are endocrine hormones that control the stress responses by activating CRF1R and CRF2R, two members of class B G-protein-coupled receptors (GPCRs). Here, we present two cryoelectron microscopy (cryo-EM) structures of UCN1-bo...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.01.013
更新日期：2020-02-06 00:00:00
abstract：:Brr2 is a unique DExD/H box protein required for catalytic activation and disassembly of the spliceosome. It contains two tandem helicase cassettes that both comprise dual RecA-like domains and a noncanonical Sec63 unit. The latter may bestow the enzyme with unique properties. We have determined crystal structures of ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.08.006
更新日期：2009-08-28 00:00:00
abstract：:Mirtrons are alternative precursors for microRNA biogenesis that were recently described in invertebrates. These short hairpin introns use splicing to bypass Drosha cleavage, which is otherwise essential for the generation of canonical animal microRNAs. Using computational and experimental strategies, we now establish...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.09.028
更新日期：2007-10-26 00:00:00
abstract：:Transcription of telomeric DNA has long been thought to inhibit telomerase. Using live cell imaging, in this issue, Cusanelli et al. (2013) now reveal a different scenario: telomeric RNA is preferentially generated at short telomeres and delivers telomerase to the chromosome end from which the transcript originated. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2013.09.011
更新日期：2013-09-26 00:00:00
abstract：:Stress granules are mRNA-protein assemblies formed from nontranslating mRNAs. Stress granules are important in the stress response and may contribute to some degenerative diseases. Here, we describe the stress granule transcriptome of yeast and mammalian cells through RNA-sequencing (RNA-seq) analysis of purified stre...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2017.10.015
更新日期：2017-11-16 00:00:00
abstract：:Mismatch repair (MMR) is initiated by MutS family proteins (MSH) that recognize DNA mismatches and recruit downstream repair factors. We used a single-molecule DNA-unzipping assay to probe interactions between S. cerevisiae MSH2-MSH6 and a variety of DNA mismatch substrates. This work revealed a high-specificity bindi...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.10.014
更新日期：2005-12-09 00:00:00
abstract：:CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreign-derived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. M...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.12.015
更新日期：2019-02-21 00:00:00
abstract：:The histone methyltransferase PR-Set7/Set8 is the sole enzyme that catalyzes monomethylation of histone H4 at K20 (H4K20me1). Previous reports document disparate evidence regarding PR-Set7 expression during the cell cycle, the biological relevance of PR-Set7 interaction with PCNA, and its role in the cell. We find tha...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.10.011
更新日期：2010-11-12 00:00:00
abstract：:HAT1 is an essential gene in mammals that acetylates newly synthesized histone H4 and is involved in chromatin assembly and genome maintenance. In this issue of Molecular Cell, Gruber et al. (2019) show that HAT1 binds to and stimulates H4 gene expression in a growth-factor- and glucose-dependent manner. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2019.08.004
更新日期：2019-08-22 00:00:00
abstract：:Polycomb repressive complex 2 (PRC2) places H3K27me3 at developmental genes and is causally implicated in keeping bivalent genes silent. It is unclear if that silence requires minimum H3K27me3 levels and how the mark transmits faithfully across mammalian somatic cell generations. Mouse intestinal cells lacking EZH2 me...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.01.017
更新日期：2020-04-02 00:00:00
abstract：:The ring-shaped hetero-oligomeric chaperonin TRiC/CCT uses ATP to fold a diverse subset of eukaryotic proteins. To define the basis of TRiC/CCT substrate recognition, we mapped the chaperonin interactions with the VHL tumor suppressor. VHL has two well-defined TRiC binding determinants. Each determinant contacts a spe...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.09.003
更新日期：2006-10-06 00:00:00
abstract：:BRAF is an oncogenic protein kinase that drives cell growth and proliferation through the MEK-ERK signaling pathway. BRAF inhibitors have demonstrated antitumor efficacy in melanoma therapy but have also been found to be associated with the development of cutaneous squamous cell carcinomas (cSCCs) in certain patients....
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2013.08.044
更新日期：2013-10-24 00:00:00
abstract：:Messenger RNAs with premature translation termination codons (PTCs) are degraded by nonsense-mediated mRNA decay (NMD). In mammals, PTCs are discriminated from physiological stop codons by a process thought to involve the splicing-dependent deposition of an exon junction complex (EJC), EJC-mediated recruitment of Upf3...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00142-4
更新日期：2003-04-01 00:00:00
abstract：:Eukaryotic cells license far more origins than are actually used for DNA replication, thereby generating a large number of dormant origins. Accumulating evidence suggests that such origins play a role in chromosome stability and tumor suppression, though the underlying mechanism is largely unknown. Here, we show that ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.02.006
更新日期：2011-03-04 00:00:00
abstract：:One of the prominent biological phenomena controlled by Wnt signaling is the expansion of cells with predefined fates. In the November 27, 2002, issue of Cell, Rosenfeld and colleagues describe an example of Wnt-controlled proliferation during genesis of the cardiac outflow tract and the pituitary gland. As a downstre...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00801-8
更新日期：2002-12-01 00:00:00
abstract：:Productive splicing of human precursor messenger RNAs (pre-mRNAs) requires the correct selection of authentic splice sites (SS) from the large pool of potential SS. Although SS consensus sequence and splicing regulatory proteins are known to influence SS usage, the mechanisms ensuring the effective suppression of cryp...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2018.08.030
更新日期：2018-11-01 00:00:00
abstract：:Allosteric communication between interacting molecules is fundamental to signal transduction and many other cellular processes. To better understand the relationship between nuclear receptor (NR) ligand positioning and the formation of the coactivator binding pocket, we investigated the determinants of ligand selectiv...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(04)00054-1
更新日期：2004-02-13 00:00:00
abstract：:In this issue of Molecular Cell, Basak et al. (2008) identify Prl-3 as a p53-inducible gene acting in DNA damage-induced cell-cycle arrest. Both deletion and overexpression of Prl-3 can be cytostatic, adding to a growing list of genes whose expression must be carefully titrated for proper cell proliferation. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2008.04.006
更新日期：2008-05-09 00:00:00
abstract：:Despite the relevance of Argonaute proteins in RNA silencing, little is known about the structural steps of small RNA loading to form RNA-induced silencing complexes (RISCs). We report the 1.9 Å crystal structure of human Argonaute4 with guide RNA. Comparison with the previously determined apo structure of Neurospora ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.06.011
更新日期：2019-08-22 00:00:00
abstract：:The L. monocytogenes protein lnlB activates phosphoinositide 3-kinase and induces phagocytosis in several mammalian cell types. The 1.86 A resolution X-ray crystal structure of the leucine-rich repeat domain of lnlB that is both necessary and sufficient to induce phagocytosis is presented here. The structure supports ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80234-8
更新日期：1999-12-01 00:00:00
abstract：:The small nuclear RNA (snRNA) genes have been widely used as a model system for understanding transcriptional regulation due to the unique aspects of their promoter structure, selectivity for either RNA polymerase (Pol) II or III, and because of their unique mechanism of termination that is tightly linked with the pro...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2013.07.003
更新日期：2013-08-22 00:00:00
abstract：:tRNAs reading four-codon families often have a modified uridine, cmo(5)U(34), at the wobble position of the anticodon. Here, we examine the effects on the decoding mechanism of a cmo(5)U modification in tRNA(1B)(Ala), anticodon C(36)G(35)cmo(5)U(34). tRNA(1B)(Ala) reads its cognate codons in a manner that is very simi...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.11.014
更新日期：2007-01-12 00:00:00
abstract：:Eukaryotic gene expression regulation involves thousands of distal regulatory elements. Understanding the quantitative contribution of individual enhancers to gene expression is critical for assessing the role of disease-associated genetic risk variants. Yet, we lack the ability to accurately link genes with their dis...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.09.005
更新日期：2020-10-15 00:00:00
abstract：:The Polycomb repressive system is an essential chromatin-based regulator of gene expression. Despite being extensively studied, how the Polycomb system selects its target genes is poorly understood, and whether its histone-modifying activities are required for transcriptional repression remains controversial. Here, we...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.12.001
更新日期：2020-02-20 00:00:00
abstract：:In budding yeast, the nuclear RNA surveillance system is active on all pre-mRNA transcripts and modulated by nutrient availability. To test the role of nuclear surveillance in reprogramming gene expression, we identified transcriptome-wide binding sites for RNA polymerase II and the exosome cofactors Mtr4 (TRAMP compl...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2017.01.005
更新日期：2017-03-02 00:00:00
abstract：:Yeast histone H2A is phosphorylated on Ser129 upon DNA damage, an event required for efficient repair. We show that phosphorylation occurs rapidly over a large region around DNA double-strand breaks (DSBs). Histone H4 acetylation is also important for DSB repair, and we found that the NuA4 HAT complex associates speci...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2004.12.003
更新日期：2004-12-22 00:00:00
