Stalled fork rescue via dormant replication origins in unchallenged S phase promotes proper chromosome segregation and tumor suppression.

abstract：:In this issue of Molecular Cell, Hogues et al. (2008) demonstrate a wholesale shift in the key regulatory protein involved in ribosomal protein (RP) synthesis during the evolution of S. cerevisiae and, en passant, raise interesting questions about the relationship between RP genes and telomeres. ...

journal_title：Molecular cell

authors： Bhattacharya A,Warner JR

更新日期：2008-03-14 00:00:00

abstract：:Clathrin-dependent endocytosis has long been presented as the only efficient mechanism by which transmembrane receptors are internalized. We selectively blocked this process using dominant-negative mutants of Eps15 and showed that clathrin-mediated endocytosis of transferrin was inhibited, while endocytosis of interle...

journal_title：Molecular cell

authors： Lamaze C,Dujeancourt A,Baba T,Lo CG,Benmerah A,Dautry-Varsat A

更新日期：2001-03-01 00:00:00

abstract：:Cardozo Gizzi et al. (2019) develop a new sequential imaging methodology (Hi-M) for observing chromosome structure in the Drosophila blastoderm and find that topological domains in single nuclei change in response to transcriptional activation. ...

journal_title：Molecular cell

authors： Larson DR

更新日期：2019-04-04 00:00:00

abstract：:Genes are transcribed in a discontinuous pattern referred to as RNA bursting, but the mechanisms regulating this process are unclear. Although many physiological signals, including glucocorticoid hormones, are pulsatile, the effects of transient stimulation on bursting are unknown. Here we characterize RNA synthesis f...

journal_title：Molecular cell

authors： Stavreva DA,Garcia DA,Fettweis G,Gudla PR,Zaki GF,Soni V,McGowan A,Williams G,Huynh A,Palangat M,Schiltz RL,Johnson TA,Presman DM,Ferguson ML,Pegoraro G,Upadhyaya A,Hager GL

更新日期：2019-09-19 00:00:00

abstract：:The splitting of chromosomes in anaphase and their delivery into the daughter cells needs to be accurately executed to maintain genome stability. Chromosome splitting requires the degradation of securin, whereas the distribution of the chromosomes into the daughter cells requires the degradation of cyclin B. We show t...

journal_title：Molecular cell

authors： Kamenz J,Mihaljev T,Kubis A,Legewie S,Hauf S

更新日期：2015-11-05 00:00:00

abstract：:Small DNA viruses force quiescent cells to reenter the cell cycle in order to replicate their DNA. We report here that the adenovirus E1A protein creates an S phase environment in quiescent cells by overcoming the nucleosomal repression of E2F-targeted genes. These genes are surrounded by Lys-9-methylated H3 histones,...

journal_title：Molecular cell

authors： Ghosh MK,Harter ML

更新日期：2003-07-01 00:00:00

abstract：:Chromatin modification by Polycomb proteins provides an essential strategy for gene silencing in higher eukaryotes. Polycomb repressive complexes (PRCs) silence key developmental regulators and are centrally integrated in the transcriptional circuitry of stem cells. PRC2 trimethylates histone H3 on lysine 27 (H3K27me3...

journal_title：Molecular cell

authors： Simon JA,Kingston RE

更新日期：2013-03-07 00:00:00

abstract：:In mammalian cells, histone deacetylase (HDAC) and Sirtuin (SIRT) are two families responsible for removing acetyl groups from acetylated proteins. Here, we describe protein deacetylation coupled with deacetylimination as a function of lysyl oxidase (LOX) family members. LOX-like 3 (Loxl3) associates with Stat3 in the...

journal_title：Molecular cell

authors： Ma L,Huang C,Wang XJ,Xin DE,Wang LS,Zou QC,Zhang YS,Tan MD,Wang YM,Zhao TC,Chatterjee D,Altura RA,Wang C,Xu YS,Yang JH,Fan YS,Han BH,Si J,Zhang X,Cheng J,Chang Z,Chin YE

更新日期：2017-01-19 00:00:00

abstract：:Mirtrons are alternative precursors for microRNA biogenesis that were recently described in invertebrates. These short hairpin introns use splicing to bypass Drosha cleavage, which is otherwise essential for the generation of canonical animal microRNAs. Using computational and experimental strategies, we now establish...

journal_title：Molecular cell

authors： Berezikov E,Chung WJ,Willis J,Cuppen E,Lai EC

更新日期：2007-10-26 00:00:00

abstract：:The interaction between pRB and E2F is critical for control of the cell cycle and apoptosis. Here we report that pRB contains two distinct E2F binding sites. The previously identified E2F binding site on pRB is necessary for stable association with E2Fs on DNA. A second E2F interaction site is located entirely within ...

journal_title：Molecular cell

authors： Dick FA,Dyson N

更新日期：2003-09-01 00:00:00

abstract：:In this issue, Takahara and Maeda (2012) discover that together, Pbp1 and sequestration of the TORC1 complex in cytoplasmic mRNP stress granules provides a negative regulatory mechanism for TORC1 signaling during stress. ...

journal_title：Molecular cell

authors： Buchan JR,Capaldi AP,Parker R

更新日期：2012-07-27 00:00:00

abstract：:Poly-ubiquitin chains direct protein substrates to the 26S proteasome, where they are removed by the deubiquitinase Rpn11 during ATP-dependent substrate degradation. Rapid deubiquitination is required for efficient degradation but must be restricted to committed substrates that are engaged with the ATPase motor to pre...

journal_title：Molecular cell

authors： Worden EJ,Dong KC,Martin A

更新日期：2017-09-07 00:00:00

abstract：:One of the earliest steps in metazoan pre-mRNA splicing involves binding of U2 snRNP auxiliary factor (U2AF) 65 KDa subunit to the polypyrimidine (Py) tract and of the 35 KDa subunit to the invariant AG dinucleotide at the intron 3' end. Here we use in vitro and in vivo depletion, as well as reconstitution assays usin...

journal_title：Molecular cell

authors： Tavanez JP,Madl T,Kooshapur H,Sattler M,Valcárcel J

更新日期：2012-02-10 00:00:00

abstract：:Oocyte quality control culls eggs with defects in meiosis. In mouse, oocyte death can be triggered by defects in chromosome synapsis and recombination, which involve repair of DNA double-strand breaks (DSBs) between homologous chromosomes. We show that RNF212, a SUMO ligase required for crossing over, also mediates oo...

journal_title：Molecular cell

authors： Qiao H,Rao HBDP,Yun Y,Sandhu S,Fong JH,Sapre M,Nguyen M,Tham A,Van BW,Chng TYH,Lee A,Hunter N

更新日期：2018-10-18 00:00:00

abstract：:In this issue of Molecular Cell, Huang et al. (2009) describe two heterotrimeric single-stranded DNA binding complexes, SOSS1 and SOSS2, that function downstream of the MRN complex to promote DNA repair and the G2/M checkpoint. ...

journal_title：Molecular cell

authors： Nam EA,Cortez D

更新日期：2009-08-14 00:00:00

abstract：:Among the five KCNQ channels, also known as the Kv7 voltage-gated potassium (Kv) channels, KCNQ2-KCNQ5 control neuronal excitability. Dysfunctions of KCNQ2-KCNQ5 are associated with neurological disorders such as epilepsy, deafness, and neuropathic pain. Here, we report the cryoelectron microscopy (cryo-EM) structures...

journal_title：Molecular cell

authors： Li T,Wu K,Yue Z,Wang Y,Zhang F,Shen H

更新日期：2021-01-07 00:00:00

abstract：:A recent paper by Gallagher et al. (2020) demonstrates that c-di-GMP controls spore formation in Streptomyces venezuelae through sequestering the sporulation sigma factor σWhiG and presents the crystal structure of a ternary complex between c-di-GMP, σWhiG, and its anti-sigma factor, RsiG. ...

journal_title：Molecular cell

authors： Chou SH,Galperin MY

更新日期：2020-02-06 00:00:00

abstract：:The activation of NF-kappaB and IKK requires an upstream kinase complex consisting of TAK1 and adaptor proteins such as TAB1, TAB2, or TAB3. TAK1 is in turn activated by TRAF6, a RING domain ubiquitin ligase that facilitates the synthesis of lysine 63-linked polyubiquitin chains. Here we present evidence that TAB2 and...

journal_title：Molecular cell

authors： Kanayama A,Seth RB,Sun L,Ea CK,Hong M,Shaito A,Chiu YH,Deng L,Chen ZJ

更新日期：2004-08-27 00:00:00

abstract：:Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engageme...

journal_title：Molecular cell

authors： Diebold-Durand ML,Lee H,Ruiz Avila LB,Noh H,Shin HC,Im H,Bock FP,Bürmann F,Durand A,Basfeld A,Ham S,Basquin J,Oh BH,Gruber S

更新日期：2017-07-20 00:00:00

abstract：:The Mre11/Rad50/Nbs1 complex initiates double-strand break repair by homologous recombination (HR). Loss of Mre11 or its nuclease activity in mouse cells is known to cause genome aberrations and cellular senescence, although the molecular basis for this phenotype is not clear. To identify the origin of these defects, ...

journal_title：Molecular cell

authors： Hoa NN,Shimizu T,Zhou ZW,Wang ZQ,Deshpande RA,Paull TT,Akter S,Tsuda M,Furuta R,Tsutsui K,Takeda S,Sasanuma H

更新日期：2016-11-03 00:00:00

abstract：:Prokaryotes use a mechanism called priming to update their CRISPR immunological memory to rapidly counter revisiting, mutated viruses, and plasmids. Here we have determined how new spacers are produced and selected for integration into the CRISPR array during priming. We show that Cas3 couples CRISPR interference to a...

journal_title：Molecular cell

authors： Künne T,Kieper SN,Bannenberg JW,Vogel AI,Miellet WR,Klein M,Depken M,Suarez-Diez M,Brouns SJ

更新日期：2016-09-01 00:00:00

abstract：:Nup98 is a component of the nuclear pore that plays its primary role in the export of RNAs. Nup98 is expressed in two forms, derived from alternate mRNA splicing. Both forms are processed into two peptides through autoproteolysis mediated by the C-terminal domain of hNup98. The three-dimensional structure of the C-ter...

journal_title：Molecular cell

authors： Hodel AE,Hodel MR,Griffis ER,Hennig KA,Ratner GA,Xu S,Powers MA

更新日期：2002-08-01 00:00:00

abstract：:The double Holliday junction (dHJ) is generally regarded to be a key intermediate of meiotic recombination, whose resolution is critical for the formation of crossover recombinants. In fission yeast, the Mus81-Eme1 endonuclease has been implicated in resolving dHJs. Consistent with this role, we show that Mus81-Eme1 i...

journal_title：Molecular cell

authors： Osman F,Dixon J,Doe CL,Whitby MC

更新日期：2003-09-01 00:00:00

abstract：:The molecular structures of crossover (CO) and noncrossover (NCO) intermediates were determined by sequencing the products formed when a gapped plasmid was repaired using a diverged chromosomal template. Analyses were done in the absence of mismatch repair (MMR) to allow efficient detection of strand-transfer intermed...

journal_title：Molecular cell

authors： Mitchel K,Zhang H,Welz-Voegele C,Jinks-Robertson S

更新日期：2010-04-23 00:00:00

abstract：:2'3'-cyclic-GMP-AMP (cGAMP) is a second messenger that activates the antiviral stimulator of interferon genes (STING) pathway. We recently identified a novel role for cGAMP as a soluble, extracellular immunotransmitter that is produced and secreted by cancer cells. Secreted cGAMP is then sensed by host cells, elicitin...

journal_title：Molecular cell

authors： Ritchie C,Cordova AF,Hess GT,Bassik MC,Li L

更新日期：2019-07-25 00:00:00

abstract：:Multisubunit protein complexes pose a challenge to the coordinated regulation of individual components. We show how the yeast transactivating factor Met4 functions as a component of the SCF(Met30) ubiquitin ligase to synchronize its own activity with cofactor assembly. Cells maintain Met4 in a dormant state by a regul...

journal_title：Molecular cell

authors： Ouni I,Flick K,Kaiser P

更新日期：2010-12-22 00:00:00

abstract：:We have used gene competition to distinguish between possible mechanisms of transcriptional activation of the genes of the human beta-globin locus. The insertion of a second beta-globin gene at different points in the locus shows that the more proximal beta gene competes more effectively for activation by the locus co...

journal_title：Molecular cell

authors： Dillon N,Trimborn T,Strouboulis J,Fraser P,Grosveld F

更新日期：1997-12-01 00:00:00

abstract：:Activation of NF-kappaB is achieved by ubiquitination and proteasome-mediated degradation of IkappaBalpha. We have detected modified IkappaBalpha, conjugated to the small ubiquitin-like protein SUMO-1, which is resistant to signal-induced degradation. In the presence of an E1 SUMO-1-activating enzyme, Ubch9 conjugated...

journal_title：Molecular cell

authors： Desterro JM,Rodriguez MS,Hay RT

更新日期：1998-08-01 00:00:00

abstract：:While much research has examined the use of glucose and glutamine by tumor cells, many cancers instead prefer to metabolize fats. Despite the pervasiveness of this phenotype, knowledge of pathways that drive fatty acid oxidation (FAO) in cancer is limited. Prolyl hydroxylase domain proteins hydroxylate substrate proli...

journal_title：Molecular cell

authors： German NJ,Yoon H,Yusuf RZ,Murphy JP,Finley LW,Laurent G,Haas W,Satterstrom FK,Guarnerio J,Zaganjor E,Santos D,Pandolfi PP,Beck AH,Gygi SP,Scadden DT,Kaelin WG Jr,Haigis MC

更新日期：2016-09-15 00:00:00

abstract：:Small heat shock proteins (sHsps) are ubiquitous molecular chaperones that prevent the aggregation of unfolding proteins during proteotoxic stress. In Caenorhabditis elegans, Sip1 is the only sHsp exclusively expressed in oocytes and embryos. Here, we demonstrate that Sip1 is essential for heat shock survival of repro...

journal_title：Molecular cell

authors： Fleckenstein T,Kastenmüller A,Stein ML,Peters C,Daake M,Krause M,Weinfurtner D,Haslbeck M,Weinkauf S,Groll M,Buchner J

更新日期：2015-06-18 00:00:00