Global Mapping of Human RNA-RNA Interactions.


:The majority of the human genome is transcribed into non-coding (nc)RNAs that lack known biological functions or else are only partially characterized. Numerous characterized ncRNAs function via base pairing with target RNA sequences to direct their biological activities, which include critical roles in RNA processing, modification, turnover, and translation. To define roles for ncRNAs, we have developed a method enabling the global-scale mapping of RNA-RNA duplexes crosslinked in vivo, "LIGation of interacting RNA followed by high-throughput sequencing" (LIGR-seq). Applying this method in human cells reveals a remarkable landscape of RNA-RNA interactions involving all major classes of ncRNA and mRNA. LIGR-seq data reveal unexpected interactions between small nucleolar (sno)RNAs and mRNAs, including those involving the orphan C/D box snoRNA, SNORD83B, that control steady-state levels of its target mRNAs. LIGR-seq thus represents a powerful approach for illuminating the functions of the myriad of uncharacterized RNAs that act via base-pairing interactions.


Mol Cell


Molecular cell


Sharma E,Sterne-Weiler T,O'Hanlon D,Blencowe BJ




Has Abstract


2016-05-19 00:00:00














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    journal_title:Molecular cell

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    authors: Joly N,Zhang N,Buck M

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Lee ST,Li Z,Wu Z,Aau M,Guan P,Karuturi RK,Liou YC,Yu Q

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    pub_type: 杂志文章


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    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Papouli E,Chen S,Davies AA,Huttner D,Krejci L,Sung P,Ulrich HD

    更新日期:2005-07-01 00:00:00

  • A Secreted RNA Binding Protein Forms RNA-Stabilizing Granules in the Honeybee Royal Jelly.

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    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Maori E,Navarro IC,Boncristiani H,Seilly DJ,Rudolph KLM,Sapetschnig A,Lin CC,Ladbury JE,Evans JD,Heeney JL,Miska EA

    更新日期:2019-05-02 00:00:00

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    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Sartorelli V,Puri PL,Hamamori Y,Ogryzko V,Chung G,Nakatani Y,Wang JY,Kedes L

    更新日期:1999-11-01 00:00:00

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    journal_title:Molecular cell

    pub_type: 杂志文章


    authors: Chi Z,Chen S,Xu T,Zhen W,Yu W,Jiang D,Guo X,Wang Z,Zhang K,Li M,Zhang J,Fang H,Yang D,Ye Q,Yang X,Lin H,Yang F,Zhang X,Wang D

    更新日期:2020-10-01 00:00:00