Abstract：
:In the Neurospora circadian system, the transcription factors White Collar-1 (WC-1) and White Collar-2 (WC-2) activate expression of frq, whose gene product inhibits its own expression. The WC proteins are thought to form an obligate complex; however, chromatin immunoprecipitation (ChIP) indicates that WC-2 binds to the frq promoter in a rhythmic fashion, whereas WC-1 is bound continuously. Small oscillations in histone acetylation are detected over the circadian cycle with a marked reduction upon light-induced activation. Nuclease accessibility experiments indicate chromatin rearrangement at the frq promoter; therefore, 19 genes with homology to ATP-dependent chromatin-remodeling enzymes were deleted and the strains examined for clock phenotypes. One gene, designated clockswitch (csw-1), is required for clock function; its product localizes to the frq promoter, is required for proper frq expression, and has an impact on chromatin structure. The data suggest that CSW-1 regulates accessibility of promoter DNA, thus generating the sharp transition from the transcriptionally active to the repressed state.
journal_name
Mol Celljournal_title
Molecular cellauthors
Belden WJ,Loros JJ,Dunlap JCdoi
10.1016/j.molcel.2007.01.010subject
Has Abstractpub_date
2007-02-23 00:00:00pages
587-600issue
4eissn
1097-2765issn
1097-4164pii
S1097-2765(07)00015-9journal_volume
25pub_type
杂志文章相关文献
MOLECULAR CELL文献大全abstract：:Translational control is frequently exerted at the stage of mRNA recruitment to the initiating ribosome. We have reconstituted mRNA recruitment to the 43S preinitiation complex (PIC) using purified S. cerevisiae components. We show that eIF3 and the eIF4 factors not only stabilize binding of mRNA to the PIC, they also...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.08.021
更新日期：2010-09-24 00:00:00
abstract：:The inner nuclear membrane (INM) is continuous with the endoplasmic reticulum (ER) but harbors a distinctive proteome essential for nuclear functions. In yeast, the Asi1/Asi2/Asi3 ubiquitin ligase complex safeguards the INM proteome through the clearance of mislocalized ER membrane proteins. How the Asi complex select...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2019.10.003
更新日期：2020-01-02 00:00:00
abstract：:It has been known for some time that stimulation of heterotrimeric G protein-coupled receptors can cause cytoskeletal reorganization. A recent report in Developmental Cell demonstrates that the activation of a tyrosine kinase, C-terminal Src kinase, by heterotrimeric G protein subunits provides the trigger for Rho-dep...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00551-8
更新日期：2002-06-01 00:00:00
abstract：:The PTEN tumor suppressor controls cell death and survival by regulating functions of various molecular targets. While the role of PTEN lipid-phosphatase activity on PtdIns(3,4,5)P3 and inhibition of PI3K pathway is well characterized, the biological relevance of PTEN protein-phosphatase activity remains undefined. He...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2020.09.027
更新日期：2020-10-15 00:00:00
abstract：:Proteins that recognize specific DNA sequences play a central role in the regulation of transcription. The tremendous increase in structural information on protein-DNA complexes has uncovered a remarkable structural diversity in DNA binding folds, while at the same time revealing common themes in binding to target sit...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/s1097-2765(01)00392-6
更新日期：2001-11-01 00:00:00
abstract：:Bcr-Abl is a dysregulated tyrosine kinase whose mechanism of activation is unclear. Here, we demonstrate that, like c-Abl, Bcr-Abl is negatively regulated through its SH3 domain. Kinase activity, transformation, and leukemogenesis by Bcr-Abl are greatly impaired by mutations of the Bcr coiled-coil domain that disrupt ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00274-0
更新日期：2003-07-01 00:00:00
abstract：:DNA breakage is intimately associated with meiotic recombination in the fission yeast Schizosaccharomyces pombe. Sites of prominent DNA breakage were found approximately 25 to approximately 200 kb apart in the genomic regions surveyed. We examined in detail a 501 kb region of chromosome I and found six sites, or tight...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(02)00452-5
更新日期：2002-02-01 00:00:00
abstract：:The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas nuclease system is a powerful tool for genome editing, and its simple programmability has enabled high-throughput genetic and epigenetic studies. These high-throughput approaches offer investigators a toolkit for functional interrogation of not...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2017.09.017
更新日期：2017-10-05 00:00:00
abstract：:Circadian rhythms in mammals are generated by a transcriptional negative feedback loop that is driven primarily by oscillations of PER and CRY, which inhibit their own transcriptional activators, CLOCK and BMAL1. Current models posit that CRY is the dominant repressor, while PER may play an accessory role. In this stu...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.10.012
更新日期：2009-11-13 00:00:00
abstract：:In yeast, histone H3/H4 exchange independent of replication is poorly understood. Here, we analyzed the deposition of histone H3 molecules, synthesized during G1, using a high-density microarray histone exchange assay. While we found that H3 exchange in coding regions requires high levels of transcription, promoters e...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2007.07.011
更新日期：2007-08-03 00:00:00
abstract：:The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II) comprises multiple heptapeptide repeats of the consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Reversible phosphorylation of Ser2, Ser5, and Ser7 during the transcription cycle mediates the sequential recruitment of transcription/RNA pr...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2011.11.006
更新日期：2012-01-13 00:00:00
abstract：:Dosage compensation in Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from Drosophila embryos, Schneider cells, and human HeLa cells. We ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2006.02.007
更新日期：2006-03-17 00:00:00
abstract：:Deletion of elongation factor-like 1 (Efl1p), a cytoplasmic GTPase homologous to the ribosomal translocases EF-G/EF-2, results in nucle(ol)ar pre-rRNA processing and pre-60S subunits export defects. Efl1p interacts genetically with Tif6p, a nucle(ol)ar protein stably associated with pre-60S subunits and required for t...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(01)00403-8
更新日期：2001-12-01 00:00:00
abstract：:The orphan nuclear receptors SF-1 and LRH-1 are constitutively active, but it remains uncertain whether their activation is hormone dependent. We report the crystal structure of the LRH-1 ligand binding domain to 2.4 A resolution and find the receptor to be a monomer that adopts an active conformation with a large but...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00236-3
更新日期：2003-06-01 00:00:00
abstract：:In this issue of Molecular Cell, Schmidt et al. (2009) untangle the interplay between the COP9 signalosome (CSN), cullin-associated and neddylation-dissociated 1 (CAND1) protein, and cullin-RING ubiquitin ligases (CRLs). ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2009.08.011
更新日期：2009-09-11 00:00:00
abstract：:The protein 53BP1 plays a central regulatory role in DNA double-strand break repair. 53BP1 relocates to chromatin by recognizing RNF168-mediated mono-ubiquitylation of histone H2A Lys15 in the nucleosome core particle dimethylated at histone H4 Lys20 (NCP-ubme). 53BP1 relocation is terminated by ubiquitin ligases RNF1...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2017.04.009
更新日期：2017-05-18 00:00:00
abstract：:In this issue of Molecular Cell, Ginno et al. (2012) describe unusual sequence features at promoter CpG islands that can lead to formation of persistent RNA-DNA hybrids (R loops), which are proposed to prevent genomic DNA methylation. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2012.03.014
更新日期：2012-03-30 00:00:00
abstract：:Innate immune responses are critical for the immediate protection against microbial infection. In Drosophila, infection leads to the rapid and robust production of antimicrobial peptides through two NF-kappaB signaling pathways-IMD and Toll. The IMD pathway is triggered by DAP-type peptidoglycan, common to most Gram-n...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2009.12.036
更新日期：2010-01-29 00:00:00
abstract：:Mammalian lefty and zebrafish antivin form a subgroup of the TGF beta superfamily. We report that mouse mutants for lefty2 have an expanded primitive streak and form excess mesoderm, a phenotype opposite to that of mutants for the TGF beta gene nodal. Analogously, overexpression of Antivin or Lefty2 in zebrafish embry...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80331-7
更新日期：1999-09-01 00:00:00
abstract：:Many genomic studies have revealed associations between the gut microbiota composition and host metabolism. These observations led to the idea that a causal relationship could exist between the microbiota and metabolic diseases, a concept supported by studies showing compositional changes in the microbial community in...
journal_title：Molecular cell
pub_type： 杂志文章,评审
doi：10.1016/j.molcel.2020.03.005
更新日期：2020-05-21 00:00:00
abstract：:Human cleavage factor I(m) (CFI(m)) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFI(m) can stimulate both cleavage and poly(A) addition, and can act to suppress poly(A) site cleavage in a sequence-depe...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(03)00453-2
更新日期：2003-12-01 00:00:00
abstract：:The subclass of cysteine proteases termed lysosomal cathepsins has long been thought to be primarily involved in end-stage protein breakdown within lysosomal compartments. Furthermore, few specific protein substrates for these proteases have been identified. We show here that cathepsin L functions in the regulation of...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(04)00209-6
更新日期：2004-04-23 00:00:00
abstract：:Expression of BRCA1 is commonly decreased in sporadic breast tumors, and this correlates with poor prognosis of breast cancer patients. Here we show that BRCA1 transcripts are selectively enriched in the Argonaute/miR-182 complex and miR-182 downregulates BRCA1 expression. Antagonizing miR-182 enhances BRCA1 protein l...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2010.12.005
更新日期：2011-01-21 00:00:00
abstract：:In this issue of Molecular Cell, Lee et al. (2009) report that SUMOylation of nuclear receptors LXRalpha and LXRbeta plays a critical role in the transrepression of IFN-gamma-induced STAT1-dependent inflammatory responses in brain astrocytes. ...
journal_title：Molecular cell
pub_type： 评论,杂志文章
doi：10.1016/j.molcel.2009.09.002
更新日期：2009-09-24 00:00:00
abstract：:We have developed a highly parallel strategy, systematic gene-to-phenotype arrays (SGPAs), to comprehensively map the genetic landscape driving molecular phenotypes of interest. By this approach, a complete yeast genetic mutant array is crossed with fluorescent reporters and imaged on membranes at high density and con...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2017.12.016
更新日期：2018-01-18 00:00:00
abstract：:The protein kinase B-RAF is mutated in approximately 7% of human cancers. Most mutations are activating, but, surprisingly, a small number have reduced kinase activity. However, the latter can still stimulate cellular signaling through the MEK-ERK pathway because they activate the related family member C-RAF. We exami...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2005.10.022
更新日期：2005-12-22 00:00:00
abstract：:Apoptosis is initiated when Bcl-2 and its prosurvival relatives are engaged by proapoptotic BH3-only proteins via interaction of its BH3 domain with a groove on the Bcl-2-like proteins. These interactions have been considered promiscuous, but our analysis of the affinity of eight BH3 peptides for five Bcl-2-like prote...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2004.12.030
更新日期：2005-02-04 00:00:00
abstract：:Histone H2B monoubiquitination (H2Bub1) is centrally involved in gene regulation. The deubiquitination module (DUBm) of the SAGA complex is a major regulator of global H2Bub1 levels, and components of this DUBm are linked to both neurodegenerative diseases and cancer. Unexpectedly, we find that ablation of USP22, the ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2016.03.030
更新日期：2016-05-19 00:00:00
abstract：:Ataxia Telangiectasia Mutated (ATM) signaling is essential for the repair of a subset of DNA double-strand breaks (DSBs); however, its precise role is unclear. Here, we show that < or =25% of DSBs require ATM signaling for repair, and this percentage correlates with increased chromatin but not damage complexity. Impor...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/j.molcel.2008.05.017
更新日期：2008-07-25 00:00:00
abstract：:In Drosophila, specification of embryonic terminal cells is controlled by the Torso receptor tyrosine kinase. Here, we analyze the molecular basis of positive (Y630) and negative (Y918) phosphotyrosine (pY) signaling sites on Torso. We find that the Drosophila homolog of RasGAP associates with pY918 and is a negative ...
journal_title：Molecular cell
pub_type： 杂志文章
doi：10.1016/s1097-2765(00)80287-7
更新日期：1998-12-01 00:00:00
