Mechanisms of Ubiquitin-Nucleosome Recognition and Regulation of 53BP1 Chromatin Recruitment by RNF168/169 and RAD18.

abstract：:The RNA exosome complex associates with nuclear and cytoplasmic cofactors to mediate the decay, surveillance, or processing of a wide variety of transcripts. In the cytoplasm, the conserved core of the exosome (Exo10) functions together with the conserved Ski complex. The interaction of S. cerevisiae Exo10 and Ski is ...

journal_title：Molecular cell

authors： Kowalinski E,Kögel A,Ebert J,Reichelt P,Stegmann E,Habermann B,Conti E

更新日期：2016-07-07 00:00:00

abstract：:The diversity of mRNA lifetimes in bacterial cells is difficult to reconcile with the relaxed cleavage site specificity of RNase E, the endonuclease most important for governing mRNA degradation. This enzyme has generally been thought to locate cleavage sites by searching freely in three dimensions. However, our resul...

journal_title：Molecular cell

authors： Richards J,Belasco JG

更新日期：2019-04-18 00:00:00

abstract：:The structural diversity of glycans on cells-the glycome-is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates. We used maps of g...

journal_title：Molecular cell

authors： Narimatsu Y,Joshi HJ,Nason R,Van Coillie J,Karlsson R,Sun L,Ye Z,Chen YH,Schjoldager KT,Steentoft C,Furukawa S,Bensing BA,Sullam PM,Thompson AJ,Paulson JC,Büll C,Adema GJ,Mandel U,Hansen L,Bennett EP,Varki A,Vak

更新日期：2019-07-25 00:00:00

abstract：:Various experiments have demonstrated a collaborative action of Myc and Ras, both in normal cell growth control as well as during oncogenesis. We now show that Ras enhances the accumulation of Myc activity by stabilizing the Myc protein. Whereas Myc has a very short half-life when produced in the absence of mitogenic ...

journal_title：Molecular cell

authors： Sears R,Leone G,DeGregori J,Nevins JR

更新日期：1999-02-01 00:00:00

abstract：:B and T cell receptor gene assembly by V(D)J recombination is tightly regulated during lymphoid development. The mechanisms involved in this regulation are poorly understood. Here we show that nucleosomal DNA is refractory to V(D)J cleavage. However, the presence of HMG1, a chromatin-associated nonhistone DNA-binding ...

journal_title：Molecular cell

authors： Kwon J,Imbalzano AN,Matthews A,Oettinger MA

更新日期：1998-12-01 00:00:00

abstract：:Members of the ISWI family of chromatin remodeling factors exhibit ATP-dependent nucleosome sliding, loading, and spacing activities in vitro. However, it is unclear which of these activities are utilized by ISWI complexes to remodel chromatin in vivo. We therefore sought to identify the mechanisms of chromatin remode...

journal_title：Molecular cell

authors： Fazzio TG,Tsukiyama T

更新日期：2003-11-01 00:00:00

abstract：:In this issue of Molecular Cell, Zhang et al. and Møller et al. independently report studies of the E. coli Hfq protein, revealing significant sequence similarities with human Sm proteins. Their findings suggest that Hfq, and the Sm and Sm-like (Lsm) proteins, may function in stabilizing interactions between RNA molec...

journal_title：Molecular cell

authors： Donahue WF,Jarrell KA

更新日期：2002-01-01 00:00:00

abstract：:In mammalian cells, histone deacetylase (HDAC) and Sirtuin (SIRT) are two families responsible for removing acetyl groups from acetylated proteins. Here, we describe protein deacetylation coupled with deacetylimination as a function of lysyl oxidase (LOX) family members. LOX-like 3 (Loxl3) associates with Stat3 in the...

journal_title：Molecular cell

authors： Ma L,Huang C,Wang XJ,Xin DE,Wang LS,Zou QC,Zhang YS,Tan MD,Wang YM,Zhao TC,Chatterjee D,Altura RA,Wang C,Xu YS,Yang JH,Fan YS,Han BH,Si J,Zhang X,Cheng J,Chang Z,Chin YE

更新日期：2017-01-19 00:00:00

abstract：:The liver plays a central role in the control of glucose homeostasis and is subject to complex regulation by substrates, insulin, and other hormones. To investigate the effect of the loss of direct insulin action in liver, we have used the Cre-loxP system to inactivate the insulin receptor gene in hepatocytes. Liver-s...

journal_title：Molecular cell

authors： Michael MD,Kulkarni RN,Postic C,Previs SF,Shulman GI,Magnuson MA,Kahn CR

更新日期：2000-07-01 00:00:00

abstract：:Cell death, as a foil to cell expansion, has become one of the most intensively studied research areas of modern biology. Almost every aspect of life is intimately enmeshed with the proper regulation of cell death, which either contributes to or forms the basis of most human disease pathogenesis. Now, with our ever-ex...

journal_title：Molecular cell

authors： Meier P,Vousden KH

更新日期：2007-12-14 00:00:00

abstract：:A remarkable paper from the de Lange lab (Wang et al., 2004) in a recent issue of Cell reveals that homologous recombination can result in the abrupt shortening of telomeres in a process that appears to involve reciprocal crossing over within the t-loop structure that protects chromosome ends. ...

journal_title：Molecular cell

authors： Haber JE

更新日期：2004-11-19 00:00:00

abstract：:In this issue of Molecular Cell, Yang et al. (2011) demonstrate that Aurora B phosphorylates ATM, leading to its mitotic activation and ability to phosphorylate Bub1 and regulate the spindle checkpoint, thus maintaining genomic integrity. ...

journal_title：Molecular cell

authors： Bihani T,Hinds PW

更新日期：2011-11-18 00:00:00

abstract：:Mammalian target of rapamycin (mTOR) activity is regulated by assembly of two functionally distinct complexes, mTORC1 and mTORC2. In syndecan-4 (S4) null endothelial cells, mTORC2 activity is reduced, resulting in decreased Akt activation, while mTORC1 activity is increased. Levels of rictor, mLST8, and mSin-1 are unc...

journal_title：Molecular cell

authors： Partovian C,Ju R,Zhuang ZW,Martin KA,Simons M

更新日期：2008-10-10 00:00:00

abstract：:HAT1 is an essential gene in mammals that acetylates newly synthesized histone H4 and is involved in chromatin assembly and genome maintenance. In this issue of Molecular Cell, Gruber et al. (2019) show that HAT1 binds to and stimulates H4 gene expression in a growth-factor- and glucose-dependent manner. ...

journal_title：Molecular cell

authors： Denu JM

更新日期：2019-08-22 00:00:00

abstract：:It has been known for some time that stimulation of heterotrimeric G protein-coupled receptors can cause cytoskeletal reorganization. A recent report in Developmental Cell demonstrates that the activation of a tyrosine kinase, C-terminal Src kinase, by heterotrimeric G protein subunits provides the trigger for Rho-dep...

journal_title：Molecular cell

authors： Luttrell LM

更新日期：2002-06-01 00:00:00

abstract：:Apoptosis is initiated when Bcl-2 and its prosurvival relatives are engaged by proapoptotic BH3-only proteins via interaction of its BH3 domain with a groove on the Bcl-2-like proteins. These interactions have been considered promiscuous, but our analysis of the affinity of eight BH3 peptides for five Bcl-2-like prote...

journal_title：Molecular cell

authors： Chen L,Willis SN,Wei A,Smith BJ,Fletcher JI,Hinds MG,Colman PM,Day CL,Adams JM,Huang DC

更新日期：2005-02-04 00:00:00

abstract：:Using Micro-C, Hsieh et al. (2020) and Krietenstein et al. (2020) investigated 3D chromatin folding in human and mouse cells at unprecedented resolution to uncover ultra-fine-scale chromatin structures that provide direct links between genome architecture, gene expression, and gene regulation. ...

journal_title：Molecular cell

authors： Chang LH,Noordermeer D

更新日期：2020-05-07 00:00:00

abstract：:Group II intron RNPs are mobile genetic elements that attack and invade duplex DNA. In this work, we monitor the invasion reaction in vitro and establish a quantitative kinetic framework for the steps of this complex cascade. We find that target site specificity is achieved after DNA binding, which occurs nonspecifica...

journal_title：Molecular cell

authors： Aizawa Y,Xiang Q,Lambowitz AM,Pyle AM

更新日期：2003-03-01 00:00:00

abstract：:Klein et al. (2020) demonstrate for the first time that small-molecule cancer therapeutics are selectively partitioned and concentrated within phase-separated nuclear condensates, providing new insights to drug efficacy and creating the opportunity for enhanced control of therapeutic targeting. ...

journal_title：Molecular cell

authors： Howard TP,Roberts CWM

更新日期：2020-08-20 00:00:00

abstract：:Ubiquitin-dependent proteolysis is an important mechanism that suppresses the beta-catenin transcription factor in cells without Wnt stimulation. A critical step in this regulatory pathway is to create a SCF(beta-TrCP) E3 ubiquitin ligase binding site for beta-catenin. Here we show that the SCF(beta-TrCP) binding site...

journal_title：Molecular cell

authors： Su Y,Fu C,Ishikawa S,Stella A,Kojima M,Shitoh K,Schreiber EM,Day BW,Liu B

更新日期：2008-12-05 00:00:00

abstract：:TMEM39A, encoding an ER-localized transmembrane protein, is a susceptibility locus for multiple autoimmune diseases. The molecular function of TMEM39A remains completely unknown. Here we demonstrated that TMEM39A, also called SUSR2, modulates autophagy activity by regulating the spatial distribution and levels of PtdI...

journal_title：Molecular cell

authors： Miao G,Zhang Y,Chen D,Zhang H

更新日期：2020-02-06 00:00:00

abstract：:Heterochromatin is refractory to DNA transactions, including repair. In a recent issue of Molecular Cell, Goodarzi et al. (2008) reveal how the central transducing kinase of the DNA damage response relieves this natural barrier by increasing heterochromatic DNA accessibility. ...

journal_title：Molecular cell

authors： Fernandez-Capetillo O,Nussenzweig A

更新日期：2008-08-08 00:00:00

abstract：:Elucidating the individual and collaborative functions of genome maintenance factors is critical for understanding how genome duplication is achieved. Here, we investigate a conserved scaffold in budding yeast, Rtt107, and its three partners: a SUMO E3 complex, a ubiquitin E3 complex, and Slx4. Biochemical and genetic...

journal_title：Molecular cell

authors： Hang LE,Peng J,Tan W,Szakal B,Menolfi D,Sheng Z,Lobachev K,Branzei D,Feng W,Zhao X

更新日期：2015-10-15 00:00:00

abstract：:Downstream core promoter elements are an expanding class of regulatory sequences that add considerable diversity to the promoter architecture of RNA polymerase II-transcribed genes. We set out to determine the factors necessary for downstream promoter element (DPE)-dependent transcription and find that, against expect...

journal_title：Molecular cell

authors： Lewis BA,Sims RJ 3rd,Lane WS,Reinberg D

更新日期：2005-05-13 00:00:00

abstract：:Eukaryotic cells compartmentalize biochemical processes in different organelles, often relying on metabolic cycles to shuttle reducing equivalents across intracellular membranes. NADPH serves as the electron carrier for the maintenance of redox homeostasis and reductive biosynthesis, with separate cytosolic and mitoch...

journal_title：Molecular cell

authors： Lewis CA,Parker SJ,Fiske BP,McCloskey D,Gui DY,Green CR,Vokes NI,Feist AM,Vander Heiden MG,Metallo CM

更新日期：2014-07-17 00:00:00

abstract：:RING ubiquitin ligases (E3) recruit ubiquitin-conjugate enzymes (E2) charged with ubiquitin (Ub) to catalyze ubiquitination. Non-covalent Ub binding to the backside of certain E2s promotes processive polyUb formation, but the mechanism remains elusive. Here, we show that backside bound Ub (Ub(B)) enhances both RING-in...

journal_title：Molecular cell

authors： Buetow L,Gabrielsen M,Anthony NG,Dou H,Patel A,Aitkenhead H,Sibbet GJ,Smith BO,Huang DT

更新日期：2015-04-16 00:00:00

abstract：:Posttranslational histone modifications play important roles in transcription and other chromatin-based processes. Compared to acetylation, methylation, and phosphorylation, very little is known about the function of histone ubiquitylation. Here, we report the purification and functional characterization of a histone ...

journal_title：Molecular cell

authors： Wang H,Zhai L,Xu J,Joo HY,Jackson S,Erdjument-Bromage H,Tempst P,Xiong Y,Zhang Y

更新日期：2006-05-05 00:00:00

abstract：:BIK protein is an initiator of mitochondrial apoptosis, and BIK expression is induced by proapoptotic signals, including DNA damage. Here, we demonstrate that 3' end processing and expression of BIK mRNA are controlled by the nuclear PI4,5P(2)-regulated poly(A) polymerase Star-PAP downstream of DNA damage. Nuclear PKC...

journal_title：Molecular cell

authors： Li W,Laishram RS,Ji Z,Barlow CA,Tian B,Anderson RA

更新日期：2012-01-13 00:00:00

abstract：:Increasing evidence suggests that tRNA levels are dynamically and specifically regulated in response to internal and external cues to modulate the cellular translational program. However, the molecular players and the mechanisms regulating the gene-specific expression of tRNAs are still unknown. Using an inducible aux...

journal_title：Molecular cell

authors： Gerber A,Ito K,Chu CS,Roeder RG

更新日期：2020-05-21 00:00:00

abstract：:Budding yeast Cdc13, Stn1, and Ten1 form the CST complex to protect telomeres from lethal DNA degradation. It remains unknown whether similar complexes are conserved in higher eukaryotes or not. Here we isolated mammalian STN1 and TEN1 homologs and CTC1 (conserved telomere maintenance component 1). The three proteins ...

journal_title：Molecular cell

authors： Miyake Y,Nakamura M,Nabetani A,Shimamura S,Tamura M,Yonehara S,Saito M,Ishikawa F

更新日期：2009-10-23 00:00:00